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Biomedical subjects

I Braveny

Publications and source records attributed to I Braveny.

70 records · Page 4Linked to original sources
PubMed1978

[A method for testing susceptibility of Haemophilus influenzae to co-trimoxazole (author's transl)].

Various methods for determining the activity of co-trimoxazole against haemophilus influenzae have been compared. The results were cortically influenced by antagonistic substances in the medium. The content of p-aminobenzoeacid and thymidine in the medium should be as low as possible. The antagonistic influences are compensated by lysed horseblood, which contains thymidine-phosphorylase. Haeminchloride and nicotinamide adenine dinucleotide (NAD) are better growth-factors than other poorly defined preparations (Supplement C, Fil-des-Enrichment, Iso-VitaleX). For the determination of the minimal inhibition concentration (MIC) we propose the microtiter technique. Likewise a modified agar diffusion test can be recommended for screening purposes. Of the 143 haemophilus influenzae strains tested against co-trimoxazole 134 (94%) were sensitive.

4-Aminobenzoic Acid↗
PubMed1978

[Indirect hemagglutination test for detection of toxoplasma antibodies (author's transl)].

A micromethod with stable reagents for the simple and rapid detection of toxoplasma antibodies with the indirect hemagglutination test (IHAT) is presented. The results obtained with this method are compared with the titers in the dye test and complement-fixation test and with two IHAT-kits recently offered by commercial firms. We propose to use the IHAT as a supplementary reaction to the methods commonly in use. The relationship between the Sabin-Feldman dye test and IHAT is dependent upon the stage of infection. A very good agreement is obtained with latent toxoplasma infections. On the contrary with some new infections (up to 6 months) the IHAT titer is clearly lower than the dye test titer. This discrepancy opens the possibility of determining the infection stage with a single serum-sample.

Antibodies↗
PubMed1978

A method of mass cultivation of Toxoplasma gondii in cell culture.

Large quantities of Toxoplasma gondii were cultivated in human larynx carcinoma cells (H.Ep 2, heteroploid). Virulent Toxoplasma gondii, strain RH, which were obtained from the peritoneal exsudate of mice 2 days after infection served as inoculum. As culture medium for the cells and for growth medium, Minimum Essential Medium (MEM) supplemented by 5% foetal calf serum and 200,000 IE/1 Penicillin plus 20 mg/1 Sulmycin was used. 2--3 days after inoculation, 150--200 times the amount of the inoculated Toxoplasmas could be harvested in one or several harvests.

Cells, Cultured↗
PubMed1978

[Experimental studies on the affinity of Toxoplasma gondii to various organs of mice (author's transl)].

In response to their alleged "uterus affinity" the organotropism of various Toxoplasma strains was investigated in the female mouse. Using quantitative histological (immunofluorescence) and serological (Sabin-Feldman-dye test, complement fixation test), we examined the brain, heart, lung, spleen, striated muscle, uterus and ovaries. The following results were obtained: All four of the Toxoplasma strains (ALT, DX, WEISS, GAIL) showed a definite neurotropism. Located in the brain were one hundred times as many cysts as in the sexual organs and ten times as many as in the reamining organs. The uterus and ovary show a low susceptibility, invasion rarely occuring. An "uterus affinity" could not be confirmed. All four Toxoplasma strains showed the same affinity for the various organs. The strain ALT showed the most virulence and formed the most cysts.

Animals↗
PubMed1976

[Different antibody tests (IgG, IgM) from children with chronical recurrent urinary tract infections (author's tranls)].

Different Antibody tests (IgG, IgM) were made from children with chronical and recurrent urinary tract infections with help of the indirect fluorescent method and were compared with the indirect hemagglutination test. The indirect hemagglutination gave absolutely higher antibody rest results, but the specifity of the immune fluorescence test is just as large. You can see the acute attacks of pyelonephritis in either the antibody response test or IHA test. It is quite important, in the beginning, to repeat the controlls of the antibody test, because the changes that could occure in the titer should be seen. The IgG antibody reactions rise slightly during the acute attacks of E. coli- and Enterococci reinfections.

Adolescent↗
PubMed1975

A new method for producing a chronic E. coli pyelonephritis in rabbits.

Chronic pyelonephritis, especially that caused by the most common invader E. coli, corresponding satisfactorily to the pathophysiology of the human disease is difficult to reproduce. We have developed a pattern of infection with which we succeeded reliably in producing a chronic E. coli pyelonephritis. This was achieved by inserting a plastic catheter into the renal pelvis. A suspension of E. coli was injected via the catheter into the renal pelvis of 32 rabbits. This pattern of infection takes a chronic progressive course and, in all cases, results in typical macroscopic and histological changes in the kidney. The bacterial excretion in the urine remains unchanged over a period of months. With the experimental procedure described, the pathogenesis and pathophysiology of chronic E. coli pyelonephritis can be clearly studied. Drugs currently used on patients can be tested for their effectiveness in long-term application.

Animals↗