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Biomedical subjects

I Andersen

Publications and source records attributed to I Andersen.

At least 55 records · Page 3Linked to original sources
PubMed1983

Epidermal nuclear immunoglobulin deposits in some connective tissue diseases: correlation with ENA antibodies.

In-vivo nuclear deposits of IgG were demonstrated by direct immunofluorescence in epidermal cells of normal skin from 6 patients with serum antibodies to an RNase-sensitive extractable nuclear antigen (ENA). Addition of complement to the skin sections showed that C3 could bind to epidermal cells with IgG deposits. A skin biopsy from a patient with polymyositis and serum antibodies to ENA, but without nuclear IgG deposits, showed nuclear binding of C3 after addition of complement to the skin sections. The clinical diagnoses of patients with immunofluorescent staining of epidermal cells were mixed connective tissue disease (MCTD) 4 cases, systemic lupus erythematosus (SLE) 2 cases, and polymyositis 1 case. No epidermal nuclear IgG deposits could be demonstrated in 5 cases of SLE, 2 cases of MCTD, one case of polymyositis, or 15 cases of rheumatoid arthritis without antibodies to ENA.

Adult↗
PubMed1983

Human response to controlled levels of toluene in six-hour exposures.

The nasal mucus flow, lung function, subjective response, and psychometric performance of 16 young healthy subjects was studied during 6-h exposures to clean air and to 10, 40 or 100 ppm of toluene under controlled conditions. The toluene exposures did not affect nasal mucus flow or lung function. At 100 ppm irritation was experienced in the eyes and in the nose. There was a significant deterioration in the perceived air quality and a significant increased odor level during all exposures to toluene. The test battery investigated visual perception, vigilance, psychomotor functions, and higher cortical functions and comprised five-choice, rotary pursuit, screw-plate, Landolt's rings, Bourdon Wiersma, multiplication, sentence comprehension, and word memory tests. In these eight tests measuring 20 parameters, no statistically significant effects of the toluene exposure occurred. For three tests (multiplication errors, Landolt's rings, and the screw plate test) there was a borderline significance (0.05% less than p less than 0.10%). The subjects felt that the tests were more difficult and strenuous during the 100-ppm exposure, for which headache, dizziness, and feeling of intoxication were significantly more often reported. The exposures to 10 and 40 ppm did not result in any adverse effects.

Adult↗
PubMed1983

Serum antibodies to Aspergillus fumigatus in patients with rheumatic diseases.

IgG and IgA serum antibodies to Aspergillus fumigatus were determined in 47 patients with rheumatic disorders, 4 patients with pulmonary aspergillosis associated with rheumatic disease, and in 36 healthy controls. Antibody titres determined by indirect immunofluorescence were comparable in patients with rheumatic diseases and in controls, except for 2 patients with IgG antibody titres within the upper range of patients with aspergillosis. IgG antibody levels to 2 partially purified A. fumigatus antigens (I and VIII), determined by enzyme-linked immunosorbent assay (ELISA), were higher in rheumatic patients than in controls and with antigen VIII the difference reached statistical significance for all subgroups of rheumatic patients. IgA antibody levels by ELISA were also increased in rheumatic patients compared with the control group: IgA levels against antigen I fell within the range of patients with aspergillosis in 7 patients with rheumatic disorders. This suggests that some rheumatic patients are more strongly sensitized to Aspergillus antigens than normal subjects.

Adolescent↗
PubMed1982

Purity of commercial non-certified European samples of Pyronin Y.

The purity of six European non-certified samples of Pyronin Y was compared with that of two American samples certified by the Biological Stain Commission. The methods used were spectrophotometry and a Methyl Green-Pyronin staining test (both as applied by the Biological Stain Commission), thin layer chromatography, mass spectrometry, determination of pH, and content of some electrolytes. It was found that none of the European batches of Pyronin Y passed the complete test as prescribed by the Biological Stain Commission. Their dye content was uniformly low (between 5 and 19%). Furthermore, thin layer chromatography and mass spectrometry revealed that two of the dye samples contained no Pyronin Y or only traces. It is concluded that assessment of an unknown sample of a dye labelled Pyronin Y should be initiated with thin layer chromatography. The pH and content of electrolytes in an aqueous solution of the dye should also be determined in order to obtain reproducible staining results. Finally, the value of the work performed by the Biological Stain Commission is underlined, although more sophisticated methods are necessary for testing the purity of dyestuffs.

Cations↗
PubMed1982

Specificities of anti-rabbit VH antibodies in mammalian and fowl antisera.

Antibodies against rabbit VH-determinants were produced by immunizing mammals and birds with rabbit IgG, IgA or VH. Most of the anti-VH produced in mammals was specific for allotype VH-determinants, but small amounts of antibody against non-allotype VH-determinants were also produced. By contrast the immunization of chickens led to the production of predominantly non-allotype VH antibody. Further analysis of the antibody specificity revealed the production of antibody against VH-determinants available in the intact immunoglobulin (H- + L-chain) as well as antibody reacting with determinants only accessible in the absence of L-chain. Antibody of the latter specificity prevailed after immunization with VH. The purified VH was not deficient in antigen determinants when compared with VH in the intact immunoglobulin (IgA). Unexpectedly, none of our chicken anti-rabbit VH antisera showed crossreactivity with VH from other mammalian species.

Animals↗
PubMed1982

Cytomegalovirus antibodies in epileptics receiving diphenylhydantoin.

Cytomegalovirus (CMV) antibodies were determined by indirect haemagglutination in 53 epileptics receiving long-term diphenylhydantoin (DPH) therapy and in 53 matched controls. Absorption of serum IgG, IgA and IgM performed in 12 sera showed that the antibodies were of the IgG class. A decreased incidence of high CMV antibody titres (greater than or equal to 320) was found in epileptics (11.3%) compared with controls (34.0%) (0.02 greater than P greater than 0.01), whereas antibody titres greater than or equal to 40 were found in 37.7% of epileptics and in 45.3% of controls (n.s.). The CMV antibody incidence and titre range were similar in patients with symptomatic (37.9%) and idiopathic (37.5%) epilepsy, suggesting that the DPH treatment was responsible for the decreased antibody occurrence in patients. No correlation between CMV antibody titres and the serum immunoglobulin levels or the DPH concentration or clearance could be established in the epileptics.

Adolescent↗
PubMed1982

Reaction against liver cells by human antiactin antibodies purified by affinity chromatography.

Human antiactin antibodies were isolated from human smooth muscle antibodies by affinity chromatography using actin extracted from rabbit striated muscle and coupled to activated CH-Sepharose 4B. The eluate contained IgG and IgM smooth muscle antibodies reacting against muscularis mucosae of the stomach and blood vessels. Further the eluate contained IgM antibodies acting against the surface of isolated hepatocytes and the periphery of hepatocytes of sections of liver cells. These results indicate that antiactin antibodies possibly react with the hepatocyte membrane as has been described with regard to other antibodies.

Actins↗
PubMed1982

Acute and subacute symptoms among workers in the printing industry.

The study population comprised 52 male printers and 52 controls. Each person was interviewed about job history, general health, and work-related symptoms. Symptoms from eyes and airways, neurological symptoms, and general symptoms were recorded. A lung function test and a measurement of the sense of smell were also carried out. The printers had significantly more eye, airway, and neurological symptoms than the controls; the main complaints being irritation of eyes, nose, throat, and a reduced sense of taste. The neurological symptoms were disorders of vision, vertigo, feeling of intoxication, and headache. Furthermore, abdominal pain and flatulence occurred more often among the printers. The symptoms showed no relation to age or job seniority, but neurological and general symptoms were related to shift work. No difference in lung function was found between the two groups. The printers had a slightly lower threshold of smell than the controls. Although the total load due to organic solvents and dust in the air was far below legal limits, the number of magnitude of symptoms experienced by the printers exceeded what is supposed when norms for workroom exposure are set. It is suggested that either the irritative effects of solvents are underestimated or the assumption of additive effects when great numbers of solvents are found does not hold true. A reduction of the number of solvents by eliminating the most toxic solvents or by using dyes without solvents is suggested.

Adult↗
PubMed1982

Nasal histology and nickel concentration in plasma and urine after improvements in the work environment at a nickel refinery in Norway.

Nasal histology and nickel concentration in the plasma and urine of nickel workers were reexamined after 4 a for an evaluation of the effect of improvements in the refining process and work conditions. Ninety-eight (31%) nickel workers who were part of a previous study were reexamined. In addition, 33 of the original 38 workers with nasal epithelial dysplasia were studied for possible regression of the histological changes. Reduction in the atmospheric content of nickel and improved hygienic conditions lowered the concentration of nickel in plasma and urine by approximately 50%. The mean histological score and frequency of dysplasia was virtually the same in the two investigations, 12 and 13%, respectively. Nasal biopsies are of limited diagnostic value in the examination of individual cases. However, the method seems to be of value in identifying and monitoring groups of persons with increased risk of developing nasal carcinoma.

Adult↗
PubMed1982

Reduction of toxic indoor emissions.

A toxicological evaluation of the emission from building materials is given for irritants, allergens, odorous substances, carcinogens and teratogens and systemic toxic agents. The experience from a notification system within the National Danish Inventory of Toxic Substances and Products shows that it is possible to effectively survey building materials and products and their areas of application. As an example, data on nearly 1500 epoxy and polyurethane products (sealants, glues/adhesives and paints/lacquers) have been to estimate the percentage of those containing (a) substances known for danger or risk of irreversible effects, (b) organic solvents (greater than 5%) or (c) chemicals with irritant properties. A labelling of products is now prescribed.

Air Pollutants↗