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Biomedical subjects

H Zhou

Publications and source records attributed to H Zhou.

At least 379 records · Page 21Linked to original sources
PubMed1999

[Significance of the changes of plasma endothelin, norepinephrine and atrial natriuretic peptide in treatment of heart failure with beta-blocker].

Thirty patients with chronic heart failure after conventional treatment for two weeks with cardiac diuretic and vasodilator were divided into two groups. There was addition of betaloc into Group A, but Group B kept the original treatment plan for 8 weeks. Group C consisted of 16 normal controls. The results showed that: 1. The level of plasma endothelin(ET), norepinephrine(NE), atrial natriuretic peptide(ANP) in Group A and B were higher than those in Group C. 2. The decreasing degrees of ET, NE, ANP, heart function score and the increasing degrees of CI and LVEF in Group A were more than those in Group B. 3. The change of ET was related to the changes of the others. Therefore, the beta-blocker may be associated with the decrease of the plasma concentration of ET, NE and ANP and improve heart function. It is considered that the degree of change of plasma ET may be the simple and reliable index which evaluate the degree of improving heart function.

Adrenergic beta-Antagonists↗
PubMed1999

[One hundred and twenty-four cases of diffuse pulmonary disease diagnosed by transbronchial lung biopsy via fiberbronchoscope].

OBJECTIVE: To understand the diagnostic value in diffuse pulmonary disease examined with transbronchial lung biopsy(TBLB) via fiber bronchoscope. METHOD: One hundred and twenty-four cases of diffuse pulmonary disease were diagnosed with TBLB via fiberbronchoscope. RESULT: Confirmed diagnosis was obtained in 91 patients; the total diagnostic rate was 73.39%. The kinds of the diseases included lung cancer, lung tuberculosis, diffuse interstitial pulmonary fibrosis, pulmonary sarcoidosis, and pulmonary lesion in collageous diseases. There were no other serious complications except for bloody or blood-streaking sputum, and pneumothorax occurred occasionally. CONCLUSION: It is suggested that TBLB is an easy, safe and useful method for diagnosing diffuse pulmonary diseases.

Adolescent↗
PubMed1999

[Preliminary study on chemical changes in the decocting process of radix et rhizoma Rhei].

OBJECTIVE: To understand the chemical changes in the decocting process of Radix et Rhizoma Rhei. METHOD: Comparing the contents of many kinds of anthraquinones in the crude drug, decoction and drug sediments of Radix et Rhizoma Rhei by HPLC. RESULT: The contents of combined and free anthraquinones changed after the crude drug was decocted. CONCLUSION: These changes may be related to the hydrolysis of combined anthraquinones, the decomposition of free anthraquinones, and the inter-transition among anthraquinones.

Anthraquinones↗
PubMed1999

[Determination of alkaloids in Coptis-Cinnamomum compatibility by capillary electrophoresis].

OBJECTIVE: To establish a HPCE method to determine the contents of alkalods in Rhizoma Coptidis. METHOD: HPCE. RESULT: The constituents thus determined have good linearity and separation. The average recovery of coptisine, berberine, palmatine and jatrorrhizine was 98.28%, 98.38%, 97.78% and 98.89% respectively and RSD 0.86%, 1.12%, 1.06% and 1.11% respectively. CONCLUSION: The method is convenient, rapid, accurate and practicable. The compatibility reduces the contents of alkaloids in Rhizoma Coptidis in different degrees.

Alkaloids↗
PubMed1999

[Changes of telomere length in human nasopharyngeal carcinoma].

Using Southern hybridization, we analysed the telomere length from thirty patients with nasopharyngeal carcinoma and demonstrated telomere reduction in these individuals when compared with their age-matched controls. Of the 30 samples, 16.67% had telomere elongation, 76.67% had telomere reduction and 6.67% exhibited equal lengths as compared with the controls. Analysis of leukocytes from 30 controls indicated that the telomere length decreased (average 39 base Pairs Per year) with aging and a statistically significant correlation between the length and age was noted (r = -0.3913, P < 0.05). These results suggest that the consequences of telomere shortening in nasopharyngeal carcinoma may lead to chromosome instability.

Adult↗
PubMed1999

[Determination of dicarboxylic acids in a new compound liquid fertilizer by gas chromatography].

A method for determination of succinic acid, glutaric acid and adipic acid in a new compound liquid fertilizer by gas chromatography is described. The sample after dried at 80 degrees C was extracted by ethyl alcohol, and then esterified in the presence of BF3. The esterified derivatives were injected into a gas chromatograph with a 5% SE-30 packed column, programmed from 140 degrees C (held for 1 min) to 160 degrees C (held for 6 min) at a rate of 6 degrees C/min. Internal standard was diethyl malonate. Calibration curves of the three diethyl esters were linear in the ranges of 2.3-9.3 micrograms, 10.8-43.2 micrograms and 2.8-11.0 micrograms respectively.

English Abstract↗
PubMed1999

[Induced-differentiation and cytotoxicity of isoverbascoside on HL-60 cells].

HL-60 cells were treated by isoverbascoside with different time and different concentrations in vitro. The differentiation of HL-60 cells was evaluated by light and electron microscopy to observe morphological changes, by chemiluminence to detect phagocytosis and by tumorigenesis in nude mice to determine malignancy. The cytotoxical effect of isoverbascoside on HL-60 cells was examined by trypan blue excluding staining and electron microscopy. The influence of isoverbascoside on cell cycle was measured by flow cytometry. Granular differentiation of HL-60 cells was induced by isoverbascoside at 20-25 mumol/L within 1-3 days as the results of morphological changes, enhancement of phagocytosis and decreasing of tumorigenesis. Strong cytotoxicity was evidenced in HL-60 cells treated by isoverbascoside at 30-35 mumol/L. HL-60 cells treated by isoverbascoside at 20 mumol/L were delayed at G1 phase at 12 hours and G2/M phase at 72 hours.

Animals↗
PubMed1999

[Gas chromatography/mass spectrometry analysis for determination of methyl alpha-(6-methoxyl-2-naphthyl) propionate from carbonylation].

alpha-(6-Methoxyl-2-naphthyl) ethanol can be carbonylated to form methyl-alpha-(6-methoxyl-2-naphthyl)propionate catalyzed by a catalyst in the pressnce of methanol. High efficiency capillary gas chromatography and GC/MS technique were used for determining products which were obtained from the carbonylation. An OV-101 fused silica capillary column (25 m x 0.2 mm i.d.) and a flame ionization detector (FID) were employed in the GC analysis. The column temperature was kept at 250 degrees C. Mass spectra were obtained by electron impact at 70 eV. The structures of four compounds were identified. The major product is methyl-alpha-(6-methoxy-2-naphthyl) propionate, and the two by-products are 2-vinyl-6-methoxynaphthalene and 1-methoxy-(6'-methoxy-2'-naphthyl)ethanol.

Anti-Inflammatory Agents, Non-Steroidal↗
PubMed1999

[An experimental study on the change of adhesion between leukocyte and endothelium in acute cerebral ischemia].

The objective of this experiment was to investigate the change of leukocyte adhesion in the focus of cerebral ischemia-reperfusion injury. Immune fluorescent microscope equipped with image tube camera and high speed recording TV sets was used to study the leukocyte adhesion in the focus of cerebral ischemia-reperfusion injury. The result showed that the density index of adherent leukocyte was significantly elevated, the fracture stress between leukocyte and endothelium dropped, and the adhesion increased in the focus of cerebral ischmic-reperfusion injury. These findings indicated that leukocyte adhesion was increased significantly and leukocytes played an important role in the cerebral ischemia-reperfusion injury.

Acute Disease↗
PubMed1999

[Computer-assisted identification of fundus lesions and a study of its reliability].

This study aimed at the use of computer for identifying fundus lesions quickly and reliably. We adopted the 'area filling' method to program in Visula C++, used the program to mesure the retina cotton-wool patches in diabetics (15 eyes), and then calculated the coefficient of variation to evaluate its reproducibility. The results showed that the coefficient of variation of the measurements ranged from 3.22% to 9.46%. In conclusion, this method is convient, quick and reliable.

Algorithms↗
PubMed1999

[Separation and analysis of amino acids in secretion of psylla chinesis yang et li].

We used ion-exchange resin column chromatography to separate amino acids in secretion of Psylla Chinese Yang et Li and used capillary gas chromatography to analysis n-butyl-N-trifluoracetyl derivatives of the amino acids. Qualitative analysis was done by comparing with the standard amino acids and quantitative analysis by internal standard method. We determined thirteen amino acids in the secretion.

English Abstract↗
PubMed1999

[Result of phacoemulsification and intraocular lens implantation combined with trabeculectomy in the treatment of glaucoma with cataract].

OBJECTIVE: The study was designed to investigate the application of Phacoemulsification and Intraocular Lens Implantation combined with Trabeculectomy (Phaco & IOL & T) for glaucoma with cataract. METHODS: "Phaco & IOL & T" was performed on 45 eyes of 45 patients in comparing with the treatment of Extra Capsule Cataract Extration and Intraocular Lens Implantation combined with Trabeculectomy (ECCE & IOL & T) on 40 eyes of 40 patients. The average period of follow-up was six months. RESULTS: The average Intra Ocular Pressure (IOP) in "Phaco & IOL & T" group was 2.08 kPa, astigmatism was 0.84 +/- 0.63 D and 84% of patients had the visual acuity > or = 1.0. The average IOP in "ECCE & IOL & T" group was 2.78 kPa, astigmatism was 1.54 +/- 0.67 D and 67.5% of patients had the visual acuity > or = 1.0 after three months of the operations. The difference was significant (P < 0.05). There was no severe complications in "Phaco & IOL & T" group while there was 9 cases with flat filtration pad in "ECCE & IOL & T" group. CONCLUSION: The method of "Phaco & IOL & T" is more efficient and safe than that of "ECCE & IOL & T". It should be the first consideration in the management of glaucoma with cataract.

Aged↗
PubMed1999

[The design of a multi-parameter patient monitor].

This paper describes the software and hardware design of a multi-parameter patient monitor which is able to measure ECG, heart rate, blood pressure, breath rate, body temperature and oxygen saturation non-invasively. The instrument uses embedded industry control module as its main controller and 9" CRT as its display. Several OEM modules are utilized in the design, such as ECG module, blood pressure module and oximeter module. This design strategy has the features of reliable performance, easy maintenance, and short developing period.

Diagnostic Equipment↗
PubMed1998

Molecular cloning and expression of human grap-2, a novel leukocyte-specific SH2- and SH3-containing adaptor-like protein that binds to gab-1.

The SH2- and SH3-containing adaptor molecules serve to recruit cytosolic signal-transducing molecules to the activated receptor tyrosine kinases. In this study, we report the molecular cloning of a novel adaptor-like protein, Grap-2 (Grb-2 related adaptor protein 2), using the multisubstrate docking protein Gab-1 as bait in the yeast two-hybrid system. Sequence analysis revealed that Grap-2 contains a SH3-SH2-SH3 structure that has a high degree of sequence homology to those of the Grb-2 and Grap adaptor molecules. However, unlike in Grap and Grb-2, the SH2 and the C-terminal SH3 domains of Grap-2 are separated by a 120-amino-acid glutamine-rich sequence that shows no apparent homology to any known molecule or structural motif. The C-terminal SH3 domain of Grap-2 alone is sufficient to bind to Gab-1. Furthermore, Northern blot analysis demonstrated that Grap-2 has two major transcripts of 1.4 and 4.0 kb that can only be detected in tissues rich in leukocytes and in two leukemia cell lines. This highly restricted pattern of expression suggests that Grap-2 may participate in leukocyte-specific protein tyrosine kinase signaling.

Adaptor Proteins, Signal Transducing↗
PubMed1998

High doses of a helper-dependent adenoviral vector yield supraphysiological levels of alpha1-antitrypsin with negligible toxicity.

Optimal gene therapy for many disorders will require efficient transfer to cells in vivo, high-level and long-term expression, and tissue-specific regulation, all in the absence of significant toxicity or inflammatory responses. While recombinant adenoviral vectors are efficient for gene transfer to hepatocytes, their usefulness is limited by short duration of expression related, at least in part, to immune responses to viral proteins and by a low capacity for foreign DNA. A number of systems have been developed for producing adenoviral vectors devoid of all viral coding sequences. Using AdSTK109, a vector lacking all viral coding sequences and carrying the complete human alpha1-antitrypsin (hAAT) genomic DNA locus, we have demonstrated sustained expression for longer than 10 months in mice. Utilizing high doses of this vector for hepatic gene transfer in mice, we find that supraphysiological levels of hAAT can be achieved without hepatotoxicity.

Adenoviridae↗
PubMed1998

Increased vascularization in mice overexpressing angiopoietin-1.

The angiopoietins and members of the vascular endothelial growth factor (VEGF) family are the only growth factors thought to be largely specific for vascular endothelial cells. Targeted gene inactivation studies in mice have shown that VEGF is necessary for the early stages of vascular development and that angiopoietin-1 is required for the later stages of vascular remodeling. Here it is shown that transgenic overexpression of angiopoietin-1 in the skin of mice produces larger, more numerous, and more highly branched vessels. These results raise the possibility that angiopoietins can be used, alone or in combination with VEGF, to promote therapeutic angiogenesis.

Angiopoietin-1↗
PubMed1998

Evaluating effects of exposures on embryo viability and uterine receptivity in in vitro fertilization.

We consider models for the occurrence of pregnancy following in vitro fertilization. In this clinical protocol, implantation depends on two factors: the receptivity of the uterus and the viability of at least one of the embryos transferred to the uterus. This work is motivated by the need to identify reliable bio-markers for these two factors, in order to enhance the success rate for couples undergoing this procedure. We present a general latent variable structure model for outcomes that take either one of two possible forms: as summed Bernoullis, based on an ultrasound count of gestational sacs, or as aggregated Bernoullis, based only on the outcome of a biochemical pregnancy test. We allow both uterine receptivity and embryo viability to be influenced by covariates. The proposed latent variable structure allows us to utilize the existing statistical packages to maximize an otherwise intractable likelihood function. The method is sufficiently flexible to permit any valid choice of link function. We illustrate by applying the method to a recent study of in vitro fertilization carried out in North Carolina. The number of cells at transfer is evidently a marker for embryo viability.

Adult↗