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Biomedical subjects

H Young

Publications and source records attributed to H Young.

At least 199 records · Page 11Linked to original sources
PubMed1981

Reactivity of the limulus lysate assay with uterine cervical secretions. A preliminary evaluation.

A limulus lysate assay was performed on cervical secretions from 66 women. When secretions were tested at a 1/100 dilution the assay gave a positive result in 15 (62.5%) of 24 patients with gonorrhoea confirmed by Gram-stained smear or culture or both. When secretions from seven of the nine remaining patients who had gonorrhoea but negative results to the limulus lysate test were retested at 1/50 dilution, two gave a positive result, increasing the positivity rate of the test to 17 (70.8%) of 24 infected patients. Material from one patient with a history of contact with gonorrhoea and from three (7.3%) of the other 41 patients without any history of gonorrhoea gave positive reactions.

Cervix Mucus↗
PubMed1981

Genitourinary infection with Ureaplasma urealyticum in women attending a sexually transmitted diseases clinic.

Ureaplasma urealyticum was detected in the cervix of 49·9% and in the urine of 47·7% of women attending a department of genitourinary medicine. Isolation of U urealyticum was not related to diagnosis, nor was there any association between the presence of the organism and any symptoms or signs of genital tract disease. Fewer organisms were detected in the cervix of women who had a concurrent infection with Chlamydia trachomatis than in women who had not. A pathological role for U urealyticum has not been established.

Adolescent↗
PubMed1980

Identification of pathogenic neisseriae by genetic transformation.

The detection of pathogenic neisseriae by genetic transformation of a naturally occurring proline auxotroph of Neisseria gonorrhoeae strain F62 is described. Of 169 clinical isolates of N. gonorrhoeae, approximately 90% gave a positive transformation assay. Twelve clinical isolates of N. meningitidis and stock cultures of the various meningococcal serogroups also gave a positive result. However, the sensitivity of the assay was found to be approximately 1000-fold lower with N. meningitidis as test organism. Eleven other members of the family Neisseriaceae failed to transform the recipient organism. Although proline requirement did not appear to limit the value of the assay greatly, it probably was the main reason for negative results. The sensitivity of the assay and its ability to detect non-viable gonococci suggests that this method merits further investigation as a possible aid to diagnosis of gonococcal infection in special circumstances.

Bacteriological Techniques↗
PubMed1980

Studies in cutaneous immunofluorescence. I. The effect of storage time on direct immunofluorescence of skin biopsies from bullous disease and lupus erythematosus.

Forty-eight biopsy samples submitted to a diagnostic immunofluorescence service have been stored at -20 degrees C and sequentially re-tested at monthly intervals to ascertain the duration for which immunoglobulins and complement can be identified in these samples in typical and diagnostic patterns of distribution. Four biopsies from pemphigus vulgaris, 14 biopsies from bullous pemphigoid, nine biopsies from dermatitis herpetiformis and 21 from chronic discoid lupus erythematosus have been studied in this manner. In all cases diagnostic immunofluorescence patterns were present up to 4 months after biopsy and in several biopsies a diagnostic pattern was observed at 9 months. It is concluded that adequate storage facilities for a diagnostic immunfluorescence service can be achieved with a -20 degrees C storage system and that known positive material can be retrieved from this system for use as controls or in research studies for a minimum of 4 months.

Biopsy↗
PubMed1980

Detection of antigonococcal IgA in cervical secretions by indirect immunofluorescence: an evaluation as a diagnostic test.

Using an indirect immunofluorescent-antibody method, antigonococcal IgA was detected in the cervical secretions of 56 (72%) of 78 women with untreated gonorrhoea but in only 25 (5%) of 490 non-infected women. The low sensitivity--comparable to that of Gram-stain microscopy--the expense, and the laboriousness of the test militate against its use as a routine diagnostic procedure.

Antibodies, Bacterial↗
PubMed1980

Oropharyngeal flora and individual susceptibility to neisserial infection.

Beta-haemolytic streptococci were isolated from throat swabs from 49 (10.5%) of 466 patients undergoing cultural examination for gonorrhoea. Although beta-haemolytic streptococci were isolated more frequently from patients with genital or anorectal gonorrhoea (15.9%) than from those without (9.2%), the difference was not statistically significant. When groupable (A, B, C, or G) and other (non-A, -B, -C, or -G) beta-haemolytic streptococci were analysed separately, as statistically significant association between non-A, -B, -C, or -G streptococci and gonococci was observed but not between groupable beta-haemolytic streptococci and gonococci.

Female↗
PubMed1980

Isolation of Neisseria lactamica from the female genital tract. A case report.

Neisseria lactamica was isolated from the genital tract of a young patient with a persistent vaginal discharge. Although infection with N lactamica occurs very rarely, the importance of complete biochemical identification of neisseriae is emphasised in view of the serious social and medicolegal consequences which could result from a misdiagnosis of gonorrhoea.

Adolescent↗
PubMed1979

Antibodies to Neisseria gonorrhoeae: a study of the urethral exudates of 232 men.

The results of a study of the antibodies that react with Neisseria gonorrhoeae in the urethral exudates of 232 men who attended a clinic of the Department of Sexually Transmitted Diseases at Glasgow, Scotland, are presented. Of 132 men with gonorrhea, antibodies to the gonococcus were identified by use of an indirect immunofluorescent antibody test in 129 (98%): IgA antibody was found in all 129 (98%), IgG antibody in 119 (90%), and IgM antibody in 64 (49%). IgA antibody to the gonococcus was found in the secretions of only one (1%) of another 100 patients who did not have gonococcal urethritis (50 had nongonococcal urethritis and 50 had no evidence of urethritis). IgG antibody was found in 13 (26%) of the 50 men with nongonococcal urethritis and in none of the 50 men who had no evidence of urethritis. When treatment of gonorrhea was successful, the titers of IgA and IgM antibody declined rapidly and were generally undetectable within 14 days; the titers of IgG declined more slowly and could still be detected 28 days after treatment.

Antibodies, Bacterial↗
PubMed1979

Inhibition of spontaneous transformation of rat embryo cells releasing endogenous type C virus by virus-specific antiserum.

Noninbred Sprague-Dawley rat embryo cell clones predictably undergo transformation after 20-30 in vitro passages following spontaneous release of endogenous rat leukemia virus (RaLV). In the presence of RaLV-specific antiserum, virus production and infectivity were reduced and transformation was delayed from 6 to 25 weeks. Transformation was not associated with an increased expression of Kirsten murine sarcoma virus-related src gene RNA.

Animals↗
PubMed1979

Sensitivity of Neisseria gonorrhoeae to partially purified R-type pyocines and a possible approach to epidemiological typing.

Strains of Neisseria gonorrhoeae from a variety of sources were examined for sensitivity to 11 partially purified R-type pyocines from Pseudomonas aeruginosa. Selective inhibition of gonococci by pyocines of Kageyama groups R1 and R5 was observed. "Matched isolates", those from consorts or different body sites of individual patients, usually had very similar pyocine-sensitivity patterns and identical sensitivities to five antibiotics tested. This study included local isolates, strains from diverse geographic regions, and strains from disseminated gonococcal infections. It also proposed a relationship between pyocine-receptor sites in the lipopolysaccharide of Ps. aeruginosa and N. gonorrhoeae. Topics needing further evaluation are discussed.

Anti-Bacterial Agents↗
PubMed1979

Serum immunoglobulin response in uncomplicated gonorrhoea.

Sera from 225 men and 140 women were examined by an indirect immunofluorescent antibody technique for antibody reactive with Neisseria gonorrhoeae. Antigonococcal IgM was demonstrated at a titre of greater than or equal to 16 in about 45% of infected, but in only 3% of non-infected, patients. Most of this antibody occurred in sera of patients who had been infected for less than 14 days. Antibody of the IgA class was found at a titre of greater than or equal to 16 in over half the infected, but in none of the non-infected, patients. IgG antibody reactive with the gonococcus was found in each infected patient at a titre of greater than or equal to 16 but in only 8% of controls. The mean log titre of this antibody was significantly higher in patients who had been infected for more than seven days than in those whose infection was of shorter duration.

Antibodies, Bacterial↗
PubMed1979

Individual susceptibility to neisserial infection?

Specimens from genital, anorectal, and pharyngeal sites from 1671 men and 1419 women were cultured for Neisseria gonorrhoeae. Pharyngeal specimens were also cultured for Neisseria meningitidis, N. gonnorrhoeae was isolated from a genital site 2.7 times more often in men and 1.8 times more in women who also carried meningococci in their pharynx than from those who did not; the meningococcus was isolated 3.4 times more often from men and 2.0 times more often from women with genital gonorrhoea than from those without. In both men and women the association of each organism with the other was statistically significant (p less than 0.001) and may be related to sexual behaviour rather than to individual susceptibility to neisserial infection.

Cervix Uteri↗