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Biomedical subjects

H Yoshida

Publications and source records attributed to H Yoshida.

At least 1,279 records · Page 71Linked to original sources
PubMed1994

[A case report of aneurysm of the diverticulum of the ductus arteriosus in the elderly].

A 82-year-old female was admitted to our department with complaint of hoarseness. Retrograde aortogram revealed an aneurysm arising from distal aortic arch. The aorta including the aneurysm was resected and replaced with woven Dacron graft under partial cardiopulmonary bypass. An Aneurysm of the diverticulum of the ductus arteriosus is rare in adult and because of its high fatality it should be resected.

Aged↗
PubMed1994

[Experience with surgery for metachronous double cancer originated from thymus and lung].

A case with thymic cancer who developed pulmonary cancer ten years after the initial surgery was reported. A 50-year-old man was admitted to our hospital due to SVC syndrome. Chest X-ray examinations including venogram showed a mass lesion at the superior mediastinum. The patient underwent surgical removal and reconstruction of SVC, followed by radio-chemotherapy. The histologic diagnosis was thymic squamous cell carcinoma. He was admitted to our hospital again ten years after the initial surgery under the diagnosis of pneumonia. Bronchoscopic examination revealed nodular lesions at the right upper lobe bronchus and lower lobe bronchus, those lesions were diagnosed with squamous cell carcinomas cytologically. Right pneumonectomy was performed and the diagnosis was confirmed pathologically. Postoperative course was uneventful as of 10 months after the operation. It was concluded that this case showed metachronous double cancer of the thymus and the lung, as well as synchronous multiple bronchogenic carcinomas, which, to our knowledge, has never been reported yet.

Carcinoma, Squamous Cell↗
PubMed1994

[Evaluation of clinical usefulness of 11C-methionine positron emission tomography (11C-MET-PET) as a tool for liver functional imaging].

We studied 11C-MET-PET in 17 clinical cases, 10 patients with obstructive jaundice and 7 normal volunteers, and analyzed its efficacy for the evaluation of hepatic functional reserve in major hepatectomy candidates. Differential absorption ratio (DAR) of 11C was compared to the hepatic protein synthesis rate (HPS), which is measured as the incorporation rate of 3H-labeled leucine in protein fraction, using needle biopsied liver specimen obtained from each hepatic segment. In the cases of normal liver function, DAR was well correlated with HPS. Also in jaundice cases with two exceptions, low HPS segment was demonstrated as low DAR segment. Consequently, MET-PET images could clearly provide functional liver imaging. After injection to 11C-MET, the increase in rate of radioactivity of 11C in plasma protein fraction was higher in jaundice cases than in normal volunteers, which is in accord with the results of our former study that cholestatic liver has accelerated protein synthesis rate. In summary, since 11C-MET-PET could demonstrate liver functional imaging, it might be a possible tool for liver function assessment in major hepatectomy candidates.

Carbon Radioisotopes↗
PubMed1994

Time-varying properties of respiratory fluctuations in pupil diameter of human eyes.

The time varying properties have been investigated of respiratory components in fluctuations of the pupil diameter (PDF) of the human eye. PDF and instantaneous lung volume were simultaneously measured for 45 minutes under natural binocular viewing conditions. The short-term Fourier transform and the autoregressive signal modeling together with the recursive least squares algorithm have been applied for the estimation of nonstationary power spectra. Data analysis showed a bursting type non-stationarity in the appearance of the respiratory component in PDF.

Adult↗
PubMed1994

[Giant intrascrotal lipoma: a case report].

A case of giant intrascrotal lipoma is presented. The patient was a 72-year-old man with the chief complaint of painless swelling in the scrotum which had been noticed about 10 years previously. In the right scrotum, an elastic soft mass with negative transillumination was palpated. Ultrasonography and X-ray film demonstrated that it was solid mass. Under the diagnosis of intrascrotal tumor, an operation was performed. As the testis, epididymis, and spermatic cord was intact, the tumor was removed. The pathological diagnosis of the tumor was a well-capsulated benign lipoma. Including the present case, 29 Japanese cases of intrascrotal lipoma are reviewed.

Aged↗
PubMed1994

[A case of hypertrophic obstructive cardiomyopathy (HOCM) complicated by infective endocarditis (IE) and mitral regurgitation (MR)].

The case of a 56-year-old man who underwent mitral valve replacement due to HOCM complicated by IE and MR is reported. Preoperative 2D echocardiographic assessment of the mitral valve revealed systole anterior motion (SAM) and vegetation, and color flow Doppler echocardiographic examination revealed severe mitral regurgitation. The left ventricular out-flow tract gradient decreased from 140 mmHg preoperatively to 60 mmHg postoperatively. Mitral valve replacement should be considered in patients with associated IE and severe MR.

Cardiomyopathy, Hypertrophic↗
PubMed1994

[Treatment of unresectable non-small cell lung cancer (NSCLC) with carboplatin and chronic daily administration of oral etoposide].

CBDCA has only modest activity against NSCLC, but it is less toxic than cisplatin (CDDP). And CB DCA has a proven synergistic effect with etoposide. On the other hand, etoposide is schedule dependent and when give daily peros has activity against several tumors. So we conducted a trial to evaluate the efficacy of a combination of CBDCA and chronic daily administration of oral etoposide for previously untreated unresectable NSCLC. The treatment schedule consisted of CBDCA 400 mg/m2 on day 1 and chronic daily administration of oral etoposide of 50 mg/day/body for 21 consecutive days every 28 days. Twenty-nine of 36 enrolled patients were eligible. The response rate was 27.6% (95% confidence interval: 11.1-43.9%). The median survival time was 405 days. The primary toxicity was myelosuppression: leukopenia, neutropenia, anemia and thrombocytopenia of Grade 3 or 4 were 14.7%, 29.4%, 26.5%, and 5.9%, respectively. No bleeding episodes or toxic death were observed. Nonhematologic toxicity was slight, and there was no severe gastrointestinal toxicity. This combination was effective against NSCLC with tolerable toxicity and an "easily tolerated outpatient regimen".

Adenocarcinoma↗
PubMed1994

High-density lipoprotein inhibits the production of platelet activating factor acetylhydrolase by HepG2 cells.

We previously demonstrated that HepG2 cells secrete platelet activating factor (PAF) acetylhydrolase, which is identical to human plasma PAF acetylhydrolase. PAF acetylhydrolase has been shown to be associated with high-density lipoproteins (HDLs) and low-density lipoproteins (LDLs) in plasma, and lipoprotein environment affects the enzyme activity. We examined the effects of HDL and LDL on the production of PAF acetylhydrolase by HepG2 cells. These lipoproteins were treated with diisopropylfluorophosphate (DFP) to inhibit completely the endogenous PAF acetylhydrolase. PAF acetylhydrolase activity in the control medium conditioned for 24 hours was 37 +/- 2.6 pmol/mg cell protein/min (mean +/- SD, n = 3). DFP-treated HDL markedly inhibited the production of PAF acetylhydrolase by HepG2 cells in a concentration-dependent manner, and 100 micrograms/ml of HDL inhibited the production by 82%. DFP-treated LDL had a slight inhibitory effect. Sulfur 35-labeled methionine-labeled PAF acetylhydrolase had a molecular weight of about 43 kd. In the presence of 100 micrograms/ml DFP-treated HDL, the labeling of PAF acetylhydrolase was decreased both in conditioned medium and in cell homogenate (to 62% and 42% of control values, respectively). DFP-treated HDL did not affect PAF acetylhydrolase activity of conditioned medium when they were mixed immediately before the assay. However, when conditioned medium was incubated for 24 hours with HDL, PAF acetylhydrolase activity was inhibited to 78% of the control value. Analysis with gel filtration chromatography disclosed that incubation with HDL resulted in the transfer of the LDL-associated enzyme to HDL fraction.(ABSTRACT TRUNCATED AT 250 WORDS)

1-Alkyl-2-acetylglycerophosphocholine Esterase↗
PubMed1994

[A case of aortic regurgitation combined with obstruction of the left coronary ostium caused by adhesion of left coronary cusp].

A case, 31-year-old female with aortic regurgitation and ostial obstruction of left coronary artery, was reported. Her chief complaint was effort angina. The coronary angiography showed obstruction of left coronary ostium with intact main stem and its branches, perfused from right coronary artery and the aortogram revealed aortic regurgitation of grade 3-4. Left Valsalva sinus was obstructed by adhesion of left coronary cusp. Each coronary cusps were resected, and aortic valve replacement with 23 mm SJM valve and coronary arterial bypass by means of in-situ graft of left internal thoracic artery underwent successfully. The pathological findings of aortic cusps were healed valvulitis without specific inflammation as syphilis or aortitis syndrome.

Adult↗
PubMed1994

[A study of pacemaker implantation to cases performed valve surgery].

For 13 years between 1980 and 1992, 23 patients needed pacemaker (PM) implantation because of bradyarrhythmia, 21 of atrial fibrillation and two of complete A-V block, after valve surgery. Five of 88 survivors (5.7%) after OMC, 11 of 227 (4.8%) after MVR, one of 169 (0.6%) after AVR, five of 67 (7.4%) after MVR + AVR and one of 15 (6.7%) after TVR underwent PM implantation in postoperative period. Two cases who had heart failure was implanted PM in early postoperative period. In late period, the mean duration between previous valve surgery and PM implantation was 6.4 years in 12 cases after initial valve surgery and 2.3 years in nine after second valve surgery. Postoperative course after PM implantation was almost good, but one case was died due to critical arrhythmia. And one case underwent re-MVR because of mitral bioprosthesis dysfunction and one, without anticoagulant after OMC, was complicated cerebral infarction.

Adult↗
PubMed1993

Expression and function of the interleukin 7 receptor in murine lymphocytes.

A monoclonal antibody, A7R34, that recognizes the high-affinity interleukin 7 receptor (IL-7Ra) and blocks the binding between IL-7 and IL-7Ra has been produced. Cell surface staining with A7R34 demonstrated that IL-7Ra is expressed in both B- and T-cell lineages. In the bone marrow, immature B-lineage cells that do not express surface IgM were IL-7Ra+. In the thymus, IL-7Ra was detected in CD4-8- T cells and also in CD4 or CD8 single-positive cells but not in CD4+8+ double-positive cells. In the peripheral lymphoid tissues, both CD4 and CD8 single-positive cells were the major cell types that express IL-7Ra. Addition of A7R34 to a long-term B-precursor-cell culture inhibited proliferation of the B-lineage cells, indicating that IL-7 is an absolute requirement for in vitro B-cell genesis. Consistent with this in vitro result, continuous injection of A7R34 into an adult mouse resulted in a decrease of B-precursor cells and also of thymocytes, whereas a considerable fraction of mature B and T cells in the peripheral tissues persisted over 2 weeks of the experiment. When A7R34 injection is started from day 14 of gestation, it is possible to produce mice that lack B cells. These results indicate that IL-7 is an essential molecule for generation of both B and T cells in murine bone marrow and thymus, respectively. Moreover, IL-7Ra would be the sole receptor system regulating these processes.

Animals↗
PubMed1993

Effects of media conditioned by a non-metastasizing human salivary gland adenocarcinoma cell clone and metastasizing clones from salivary gland and various other tissues on the proliferation, migration and protease production of bovine aortic endothelial cells in vitro.

We demonstrate the role in tumor-associated angiogenesis of factors released into conditioned medium (CM) from in vitro human salivary gland cell clones with biological phenotypes ranging from non-metastasizing to metastasizing. A non-metastasizing human salivary gland adenocarcinoma cell clone HSGc and its subclone with metastatic potential (Gc2-100 cl-1) were employed. We also used metastasizing cell clones obtained by explant cultures of organs of Gc2-100 cl-1 tumor-bearing nude mouse. The proliferation and migration of bovine aortic endothelial (BAE) cells were significantly stimulated by the addition of CM obtained from Gc2-100 cl-1 and metastasizing cell clones, while CM from HSGc was ineffective. When the effect on protease secretion by BAE cells was examined, CM from Gc2-100 cl-1 and metastasizing cell clones inhibited the secretion of type IV collagenases by BAE cells much more than did CM from HSGc. These findings, therefore, may imply that Gc2-100 cl-1 and metastasizing cell clones secrete angiogenic factors that stimulate not only the proliferation and migration of endothelial cells but also the formation of basement membrane components necessary for the reconstruction of new blood vessels at migrated sites of endothelial cells by preventing the degradation of basement membrane component, type IV collagen.

Adenocarcinoma↗
PubMed1993

Cardiac hypertrophy in juvenile visceral steatosis (jvs) mice with systemic carnitine deficiency.

We have reported the clinical and biochemical findings in juvenile visceral steatosis (jvs) mice with systemic carnitine deficiency. This paper is the first report about cardiomyopathy in jvs mice. Adult jvs mice (at the age of 2-3 months) show cardiac hypertrophy which is caused by enlargement of the cardiac muscle cell associated with increases of non-collagen protein and DNA content. Carnitine administration (2 mg/head, twice a day, from 1 month of age) significantly suppresses the cardiac hypertrophy, showing that carnitine deficiency plays an important role in the development of the cardiac hypertrophy. The discovery of cardiac hypertrophy in carnitine-deficient jvs mice will lead to clarification of the pathophysiology of cardiomyopathy in systemic carnitine deficiency in human beings.

Animals↗
PubMed1993

Role of plasminogen activators, metalloproteinases and the tissue inhibitor of metalloproteinase-1 in the metastatic process of human salivary-gland adenocarcinoma cells.

An in vitro system has been established in which conversion from non-metastasizing to metastasizing adenocarcinoma cells can be induced, and subsequently subjected to analysis of the expression of proteases and tissue inhibitor of metalloproteinases-1 (TIMP-1). A human salivary-gland adenocarcinoma cell clone HSGc, with no metastatic ability, was exposed to N-methyl-N-nitrosourea (MNU). Following exposure to MNU, cells with altered morphology were cloned. Upon s.c. inoculation into nude mice, MNU-treated HSGc clones formed metastatic foci in various organs, and then 5 metastasizing clones were isolated. Evaluation of expression of tissue-type plasminogen activator (tPA), urokinase-type plasminogen activator (uPA), metalloproteinases and TIMP-1 was performed by means of enzyme immunoassay, zymogram, or immunoblot. MNU-treated HSGc and metastasizing clones were found to secrete high levels of tPA, while HSGc produced undetectable levels of this enzyme. Expression of uPA was not observed in any of the cell clones. When the secretion of gelatinolytic enzymes was examined, metastasizing clones produced higher levels of 57- and 32-kDa, but not of 92- or 72-kDa gelatinases, as compared to HSGc cells. Although TIMP-1 was detected in all cell clones, metastasizing clones secreted less TIMP-1 than HSGc cells; in addition, one metastasizing clone produced TIMP-1 with a molecular weight distinct from that of 28-kDa TIMP-1. Our results suggest that the acquisition of metastatic ability by human salivary-gland tumor cells is closely associated with increased secretion of several metalloproteinases as well as decreased or altered TIMP-1 expression.

Adenocarcinoma↗
PubMed1993

Improved enzyme immunoassay for human basic fibroblast growth factor using a new enhanced chemiluminescence system.

An enhanced chemiluminescence reaction has been incorporated into an enzyme immunoassay (EIA) for human basic fibroblast growth factor (hbFGF). We developed a new luminol derivative, designated L-012 and a new enhancer, 4-(4-hydroxyphenyl)thiazole. Using these compounds, the detection limit of hbFGF was improved to 0.1 pg/assay, which was 10-20 and 2 times better than the o-phenylenediamine colorimetric and luminol chemiluminescence assays, respectively. The average concentration of bFGF in sera from 25 normal volunteers was 5.9 pg/ml. On the other hand, serum bFGF levels were elevated in renal, lung and brain tumor patients. The data presented here indicate that the serum bFGF level could be a useful diagnostic marker for these tumors. Furthermore, these new compounds could easily be applied to any other EIA that uses horse radish peroxidase to improve sensitivity.

Antibodies, Monoclonal↗
PubMed1993

A new sensitive chemiluminescence probe, L-012, for measuring the production of superoxide anion by cells.

A sensitive chemiluminescence method for measuring the production of superoxide anion (O2-) by activated EoL-1 cells (human eosinophilic leukemia cell line) is described. Recently, we succeeded in synthesizing a new chemiluminescence probe, 8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine-1,4(2H,3H)dione (L-012). In the presence of L-012, activated EoL-1 cells which produce reactive oxygen species generated a marked chemiluminescence with negligible background. The L-012-dependent chemiluminescence was completely abolished by 100-300 U/ml superoxide dismutase, indicating that the main reactive oxygen species detected in this reaction was O2-. The light intensity and the sensitivity of L-012 to O2- were higher than those of other chemiluminescence probes such as luminol and Cypridina luciferin analog (MCLA). Thus, L-012 would provide an improved chemiluminescence method for measuring O2- from cells.

Complement C5a↗