[Current status and prospect of the treatment of systemic lupus erythematosus].
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Cyclophosphamide↗
Biomedical subjects
H Yang
Publications and source records attributed to H Yang.
[The effect of cardiopulmonary bypass on expression of myocardial nitric oxide synthase in patients for valve replacement].
The objective of this study was to assess the effect of cardiopulmonary bypass (CPB) on expression of nitric oxide synthase (NOS) in patients for valve replacement. Ten adult patients were included in this study. CPB was initiated with a bubble oxygenator, aorta was clamped, and cold crystalliod cardioplegia was delivered into the aortic root. The myocardium of atrial appendage was obtained pre-CPB and 45 minutes post-CPB. The expression of three kinds of NOS isoenzymes was assessed by immunohistochemistry and microcomputerized image analysis. The results demonstrate that there are expression of nNOS (nervous NOS), iNOS (inducible NOS) and eNOS (endothelial NOS) in the atrial myocardium of patients for valve replacement. Compared with the expressions of NOS pre-CPB, the post-CPB expression of nNOS increased and that of eNOS decreased (P < 0.05), but no significant change in expression of iNOS was noted(P > 0.5). We belive that the decreased expression of eNOS may be related with the deficiency of nitric oxide release and may be responsible for tissue damage during CPB.
Adult↗
[Effects of YMB injection on hemorheology in rats].
This study was designed to elucidate the mechanism of YMB Injection on anti-CAD therapy. Fufang Danshen Injection was used as positive control and saline was taken as negative control to study the effects of traditional medicine YMB Injection on hemorheological parameters in normal rats and hypostasis rats. The results showed YMB Injection had no effect on hemorheological parameters of normal rats, but it could effectively decrease whole blood viscosity and whole blood reduction viscosity of hypostasis rats. The data implied that YMB Injection could decrease blood viscosity and hence improve blood supply of the graft in anti-CAD therapy.
Animals↗
[Properties and infiltration arts of machinable infiltration ceramic(MIC)].
OBJECTIVE: The purpose of this study is to explore the infiltration arts of MIC and study the effects of different packing density of Al2O3 matrix on the properties of MIC. METHODS: alpha-Al2O3 specimens were fabricated by pouring alpha-Al2O3 slip with different powder/liquid ratios(P/L = 3.5, 7.5, 10.5) into a mold, and subsequently pre-fired at 1160 degrees C for 6 hours to form Al2O3 matrix. The packing density of the matrices were measured. Infiltration concepts were introduced into this study by infiltrating molten mica micro-crystalline glass into the porous Al2O3 matrix at 1160 degrees C for 6 hours to form a continuous interpenetrating composite. The composite then underwent micro-crystallization by nucleating at 550 degrees C for 1 hour and crystallizing at 900 degrees C for 1 hour, which resulted in the MIC. Mechanical properties including three point flexural strength, elastic modulus, Vicker's hardness, indentation fracture toughness and Weibull's modulus of flexural strength were determined. Parameters of machinability(H/KIC)2 of MIC were calculated. XRD and SEM were employed to study its microstructure. RESULTS: The resulted matrices reached packing densities of 63%, 76%, 78% with P/L of 3.5, 7.5 and 10.5. The MIC attained high strength and good machinability after infiltration. Three-point flexural strength and indentation fracture toughness were 342, 431, 374 MPa and 4.05, 4.14, 5.02 MPa m1/2 for MIC with packing density of 63%, 76%, 78% separately. And parameters of machinability were 5.41, 6.84 and 7.39 respectively. Packing density of Al2O3 matrix significantly influenced the mechanical properties. Maximum properties were obtained with a matrix packing density of 75%(P/L = 7.5), with a Weibull's modulus of flexural strength of 6.8. Machinability decreased with the increase of P/L ratio. Micro-crystallizing treatment resulted in the formation of evenly distributed mica crystalline in the composite, which contributed to the high strength of this composite material. CONCLUSION: MIC is a new infiltrated ceramic with favorable strength and machinability which can satisfy the prosthodontic requirements as all ceramic crown and bridge materials, it also shows promising outlook for future developments and clinical usage.
Aluminum Oxide↗
[Clinical study of effects of submaxillary transcutaneous electrical stimulation of genioglossus on obstructive sleep apnea syndrome].
OBJECTIVE: To investigate the effect of submaxillary transcutaneous electric stimulation of genioglossus on obstructive sleep apnea syndrome (OSAS). METHOD: Nineteen patients (male) with OSAS were diagnosed by polysomnography previously. Fixed the provocative locus of genioglossus and its controlling nerve with local anatomy. Estimated obstructive level of pharyngeal cavity by observing muller's maneuver under fiberolaryngoscope. Determined the effects of electric stimulation on different levels of narrow pharyngeal cavity with control study method. RESULT: The effect of electrical stimulation was different in different narrow level. The best effect was found on the obstruction of retrotongue-base-pharynx level, data of breath disorder (P < 0.05), oxygen saturation (P < 0.05) and clinical symptoms were significantly improved. The data were partly improved on multilevel obstructions of retrotongue-base-pharynx or above, no improvement was found on the obstruction of retrotongue-base-pharynx level. Dormant index was not worse. CONCLUSION: Submaxillary electrical stimulation is effective to the treatment of OSAS and its curative effect perhaps has a close relationship with obstructive level of with upper-airway. Further research on the mechanism showed that stimulating upper-airway dilating muscle which formed mainly by genioglossus could push the tongue ahead and effectively open pharyngeal cavity. Maybe it is a reason that the best effect took place on the retrotongue-base-pharynx level. Sleep was not affected by electrical stimulation.
Adult↗
[Study on IGF-I regulation the proliferation of rat condylar chondrocytes in vitro].
This study was aimed at the mechanism for the changes in the proliferation of cultured rat mandibular condylar chondrocytes. Flow cytometry was used to explore the changes of cellular DNA content and cell cycles of passaged condylar chondrocytes. The results showed that when the mandibular condylar chondrocytes were cultured with IGF-I for 24 hours and 96 hours, the cellular proliferation in the experimental group enhanced higher than that in the control group. It was implied that IGF-I may be a mitogen on rat mandibular condylar chondrocytes. The mitogenic effect of IGF-I had a lag period.
Animals↗
[Determination of blood glucose by chemiluminescent flow-injection analysis with immobilized glucoxidase column].
Luminol-hemin system was used for chemiluminescent flow-injection analysis with immobilized glucoxidase(GOD) column to determine blood glucose. The method is fast, simple and sensitive. Blood samples were diluted to 1:1000 and pumped into the flow-injection analyzer for direct detection. The sampling frequency was 30 samples/h, with a detection limit of 7.2 x 10(-9) mol/L(1.30 micrograms/L). A column packed with 36 milligrams of CPG could be used nearly 200 times. The determination results of glucose in serum by this method were in good agreement with those obtained by the routine GOD spectrophotometric method (correlation coefficient r = 0.90, P > 0.05). Recoveries of this method were 94.0%-107.2%.
Blood Glucose↗
[Characterization and phylogenic analysis on a new isolate of genus Rhodocista].
A strain of purple non sulfur bacteria was isolated from waste water plant in Beijing. It is phototaxic, and can converted the vibrioid cells into thick-wall cyst. Internal synthetic membrane is present as lamellae. It has a low absorption maxima at 798 nm. and require thiamine and vitamin B12 as growth factors. It can use succinate as carbon and energy source, but glucose can not be used. It can not grow in 3% NaCl and had Q-9 as major quinone. The 16S rRNA gene was amplified, cloned and sequenced. A phylogenic tree was constructed on the basis of the 16S rDNA sequences. It showed that the previously known member of the genus Rhodocista, Rc. centenaria, is the nearest neighbor to strain 3-p. The level of binary sequence similarity between Rc. centenaria and strain 3-p of 95%, and their phenotypic differences and genetic DNA-DNA relatedness of 56%, shows they are different species of Rhodocista. A new species name, Rhodocista pekinense, was proposed for strain 3-p.
Base Sequence↗
[A Carnobacterium-like organism isolated form a patient with multiple bacterial synergistic gangrene].
An atypical lactic-acid producing gram positive rod Y6 strain was studied in this report, which was isolated form clinic sample of a patient with multiple bacterial synergistic gangrene, and could not be identified by routine method. A 1.5 kb of 16S rDNA of Y6 strain was synthesized and sequenced. Comparative 16S rDNA sequence analyses revealed that stain Y6 is most closely related to the genus of Carnobacterium. The overall similarity value between Y6 strain and Carnobacterium species are 93% to 97%. The signature nucleotides in 16S rDNA primary sequence of strain Y6 and that of genus of Carnobacterium were identical. The biological features of Y6 strain are very similar to that of Carnobacterium, such as lactic acid as main end product of in PYG broth, no gas produced form fermentation of glucose, catalase negative, no motility. Data suggested that Y6 strain is very similar to the genus of Carnobacterium, of which no strain has been isolated form clinical sample so far. Based on the results obtained, we names Y6 strain as Carnobacterium-like organism.
Base Sequence↗
[Studies on detection methods of two DNA probes in Mycobacterium tuberculosis].
Two different biotinylated DNA probes which are highly specific to M. tuberculosis(MT) were made and studied. One probe is a 20 bp oligonucleotide labeled with biotin at 5' end, the other is a long DNA probe produced by PCR amplification procedure allowed for the incorporation of biotin labeled UTP. The two probes were hybridized with MT genome DNA and a 317 bp PCR product amplified from IS6110 sequence of MT, and then detected by alkaline phosphatase conjugates through colorimetric reaction. The detection sensitivity and specificity of the two probes were comparatively studied. The hybridization condition including concentration of probe, temperature of hybridization and washing filter thereafter were also investigated preliminary. The detection limit of the oligonucleotide probe and the 188 bp PCR probe were 100 ng and 6 ng of DNA respectively in detection of M. T genome, and 400 pg and 50 pg of DNA respectively in detection of PCR products of MT. The two probes can be only hybridized to MT and BCG, but not with other 24 mycobacterium or non-mycobacterium tested. The optimal hybridization temperature and washing filter temperature of oligonucleotide were 42 degrees C and 60 degrees C respectively; and that of 188 bp probe, 68 degrees C and 60 degrees C-68 degrees C. Generally the specificity of two probes were all high, but the sensitivity of 188 bp DNA probe was 7-16 times that of the oligonucleotide probe. The higher sensitivity, lower hybridization background and faster revelation of the 188 bp DNA probe made it a better choice in detection of MT.
Biotin↗
[Pathogenic mechanism of entomopathogenic bacteria--Xenorabdus and Photorhabdus].
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Animals↗
[Phenotypic features and genetic diversity of strains of Rhodopseudomonas].
14 strains of purple non-sulfur bacteria and 3 type strains of Rhodopseudomonas were investigated by phenotypic characterizing, DNA-DNA hybridizing and DGGE analyzing. The characteristics of their morphology, structure and physiology show that 14 strains of isolates have the features of the genus Rhodopseudomonas: dividing asymmetrically by budding, lamellar intracytoplasmic membranes, presence of bacteriochlorophyll a and carotenoids of the normal spirilloxanthin series. On the base of their differences in morphology, growth, carbon sources 14 strains can be divided into 2 groups: g-c group, T group. Parts of 16S rRNA gene were amplified by using a pair of primers: 341f-906r. Denaturing gradient gel electrophoresis(DGGE) of PCR-amplified fragment of rDNA indicated that they have 3 genetic types: 1 of them, including 6 of 8 strains of T groups is similar to that of Rps. rutila R1, the other 2 types, represented separately by 2 of T group or all the g-c group, were different from that of either of 3 type strains The Data of the DNA-DNA hybridization of 8 representative strains indicated that the differences between different types were enough to separate them into different species including 2 possible new species.
DNA, Bacterial↗
[Studies on sanH and sanI--genes related to nikkomycin biosynthesis of Streptomyces ansochromogenes].
A 7.0 kb DNA fragment was cloned by reverse genetics, which was located in the gene cluster of nikkomycin biosynthesis of Streptomyces ansochromogenes. Besides sanF--a gene related to nikkomycin biosynthesis of S. ansochromogenes, the sequence of a 2.2 kb BglII DNA fragment upstream sanF was determined. This 2.2 kb DNA fragment cointains two complete open reading frames (ORF). ORF1 consists of 1233 base pairs, ORF2 consistis of 195 base pairs, and they encode proteins with 410 amino acids and 64 amino acids respectively, which were designated as sanH and sanI. In search of databases, the deduced products of sanH has 46% amino acid identities and 62% amino acid similarities in comparison with the cytochrome P450 of Streptomyces griseolus, and the deduced protein of sanI has 56% amino acid identities and 70% amino acid similarities with the ferredoxin of S. griseolus. The function of sanH gene was studied using strategy of gene disruption, and the result showed that the disruption of sanH didn't influence nikkomycin biological activity in S. ansochromogenes.
Amino Acid Sequence↗
[An investigation of bioartificial pancreas for treatment of diabetes mellitus].
Bioartificial pancreas(BAP) made up of rat islet, agarose and collagen was developed in this experiment to cure the diabetes mellitus and prevent immune rejection of the islet xenograft. The insulin secretion function, immunoprotection and effect of BAP on the treatment of diabetes mellitus in mice were investigated. The results showed that the BAP maintained the insulin secretion function of islet, decreased the immunogenicity of islet xenograft and normalized the metabolic disturbances of carbohydrate in diabetic mice. These indicate that the BAP composed of the islet, agarose and collagen is an alternate approach to therapy for diabetes mellitus and to remission of immune rejection of the islet xenograft.
Animals↗
[The position of submaxillary transcutaneous electrical stimulation for obstructive sleep apnea syndrome].
OBJECTIVE: To investigate the effective position of submaxillary transcutaneous electrical stimulation for obstructive sleep apnea syndrome (OSAS). METHODS: With fixed provocative locus of genioglossus and its controlling nerve, 11 normal healthy men and 9 patients with OSAS during awakening were studied to disclose the effect of submaxillary constant-current stimulation under different stimulative radius or intensity and thickness of submaxillary skin. The effects of electric stimulation in 9 OSAS patients with narrowing of the retrotongue-base-pharynx level were compared with the control during sleep. RESULTS: The submental and internal side of mandibular angle was effective loci for stimulating genioglossus. Stimulative radius and intensity of both loci were not significantly different (P < 0.05) and no influence of the skin thickness was found. During sleep, indices of breath disorder (P < 0.05), oxygen saturation (P < 0.05) and clinical symptoms had significantly improved. CONCLUSION: Submaxillary electrical stimulation with fixed provocative locus is effective for the treatment of OSAS. The mechanism is perhaps stimulation of genioglossus pushes the tongue ahead and opens the pharyngeal cavity.
Adult↗
[Long-term follow-up result of partial laryngectomy].
OBJECTIVE: To study the long-term follow-up result of partial laryngectomy and reservation of laryngeal function. METHODS: Three hundred and seventy-nine patients who underwent partial laryngectomy from 1986 to 1995 were summarized (male 290 cases, female 89 cases). Among them, 184 cases were supraglottic carcinomas (T1 8 cases, T2 115, T3 48, T4 13, according to UICC in 1992), 192 cases were glottic carcinomas (T1 115, T2 63, T3 13, T4 1), 3 cases were transglottic carcinomas (T2 1, T3 2). In common 8 kinds of operations were performed: 26 cases underwent cordectomy, 138 vertical laryngectomy, 7 frontolateral laryngectomy, 12 horizontal glottic laryngectomy (middle part of the larynx), 58 supraglottic laryngectomy, 95 horizontovertical (3/4) laryngectomy, 24 subtotal laryngectomy with cricoglossoepiglottic anastomosis, 19 near-total laryngectomy with cricoglossal anastomosis (with reservation of unilateral arytenoid cartilage). 193 cases underwent concurrent neck dissection(121 unilateral, 72 bilateral). RESULTS: All cases restored their phonation and overcame aspiration with removing nasal feeding from 7 to 23 days after operations. 362 cases were decannulated from 9 days to 3 months after operations. Another 8 cases were decannulated after a secondary plastic operation. Decannulation rate was 97.6%. The three, five and ten year survival rates were 86.8% (329/379), 81.3% (266/327) and 69.4% (120/173) respectively. CONCLUSION: Partial laryngectomy is a kind of radical operation with reservation of laryngeal function. Mastering indications strictly, correct operation choices, excellent surgical skills and perfect repairing technique are bases of improving life qualities and curative effect.
Adult↗
[Clinical characteristics of different cordectomy types with potassium titanium phosphate laser].
OBJECTIVE: To study the clinical characteristics of different potassium titanium phosphate(KTP) laser types and surgical the recuperating course of cordectomy under suspension laryngoscope. METHOD: Follow-up observation were applied on 76 patients with cordectomy. Restoration of laryngeal structure and change of the quality of voice were observed and recorded. RESULTS: Type 1, normal laryngeal structure and function were restored postoperatively after 3 months; Type 2, a fold like vocal cord (new vocal cord) appeared 3 months later. Laryngeal structure was basically restored; Type 3, a mucosal fold appeared postoperatively after 6 months in most patients, but it lost motion function. Recurrence time was mainly found 2-4 months. Recurrence rate was 6.7%. Two and 5-year survival rate were 100%. CONCLUSIONS: Cordectomy with KTP laser can result in good effect and less injury. Type 2, 3 can retain thyroarytenoid muscle as much as possible on the basis of radical cure.
Adult↗
[Use of the oblique third mandibular molar as the abutment for fixed bridges].
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Journal Article↗