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Biomedical subjects

H Yamane

Publications and source records attributed to H Yamane.

At least 145 records · Page 8Linked to original sources
PubMed1988

Subsets of tonsillar lymphocytes and activated cells in each subset analysed by three-color flow cytometry.

Among the four subsets of tonsillar T cells, active cells were most abundant in the helper T subset, followed by the cytotoxic T, inducer T, and suppressor T subsets in order of decreasing cell count. The number of active cells was very small in the suppressor T subset. Plasma cells (PCA-1-positive cells) accounted for only about 3% of tonsillar cells. However, nearly half of the tonsillar B cells were activated (positive for Ba, being either Ba+/IgD+ or Ba+/IgD-). Ba-positive cells were hardly found in the population of peripheral-blood B cells. Ba+/IgD+ cells, which are ready to be transformed to cyclic cells or dormant cells depending upon the degree of stimulation, outnumbered cyclic cells. Many Ba+/IgD+ cells were present even in the small tonsils of adults, although these cells were very scarce in the peripheral blood. These findings show that the tonsil is prepared to perform immunologic functions in prompt response to external stimuli.

Adolescent↗
PubMed1987

Base liberation from nucleotides by superoxide and intramolecular enhancement effect of phosphate group.

The reaction of nucleotides with superoxide gave the corresponding nucleobases in good yield. The base-release reaction of nucleotides due to superoxide was examined and compared to that of nucleosides. Hence, the phosphate moiety greatly enhances the yields, in particular those of adenine from adenosine monophosphates. Superoxide in combination with the phosphate moiety has been revealed to be more active than superoxide alone. The phosphate peroxy radical, generated in situ from superoxide and phosphate, seems to be the species responsible for the formation of the free bases.

Adenine↗
PubMed1987

The occurrence of IgG in the endolymphatic sac of the guinea pig.

We investigated the occurrence of immunoglobulin G (IgG) in the endolymphatic sac (ES) of the guinea pig in order to show that the ES is an organ involved in the immunoreactivity of the inner ear. By using an immunohistological technique, we were able to show that IgG is located mainly in the subepithelial layer of the ES. We also found that IgG is present in some epithelial cells of the ES as well as in free floating cells in the ES lumen. Although plasma cells have been described previously in the subepithelial layer, none could be recognized in this layer of the ES in our specimens. Our results suggest that the ES is an immunoreactive organ of the inner ear in some pathological states, while IgG in the normal ES is primarily of systemic origin.

Animals↗
PubMed1987

Morphological changes of intercellular junctions in the rat submandibular gland treated by long-term repeated administration of isoproterenol.

Long-term repeated administration of isoproterenol (IPR) 2 mg/100 g bw, once daily for ten days, resulted in morphological changes in the intercellular junctions of rat submandibular glands, which were investigated by means of the freeze-fracture technique. A significantly increased number of tight-junctional strands was present. These junctional strands extended much deeper toward the basal membrane than those in normal acinar cells. The basal frontier strands that branched from the networks of tight junctions were elongated and had either free-endings or terminal loops, which were more frequently observed in the IPR-treated acinar cells than in untreated acinar cells. Some of the strands of tight junctions were connected to small gap junctions. The diameters of gap junctions were not significantly different from those of control acinar cells. However, smooth areas devoid of particles were found intermingling with the usual packed particles in irregularly shaped small gap junctions. There was no significant difference between the desmosomes of IPR-treated and untreated acinar cells, in terms of either morphology or distribution. These changes in junctional morphology in the IPR-treated acinar cells resemble those seen in salivary glands during development, and in some experimental conditions including tumorous changes.

Animals↗
PubMed1986

Metabolic pathway of 2-deoxy-2-fluoro-D-glucose studied by F-19 NMR.

The behavior of 2-deoxy-2-fluoro-D-glucose (FDG) in mouse has been studied by F-19 NMR method for long period. The F-19 NMR signals of FDG or its metabolites were observed in tissues without serious broadening. FDG was found to be accumulated in organs in the form of FDG or FDG-6-phosphate and 2-deoxy-2-fluoro-D-mannose (FDM) or FDM-6-phosphate, and the latter dominated the former in the heart sampled at 24 hr or later. The fluorine compounds were excreted in urine in both forms. The clearance was rapid from brain, liver, and blood, but was slow from heart.

Animals↗
PubMed1986

Enhanced production of gamma-interferon by therapy with parenteral OK-432 and alpha-interferon in patients with head and neck cancer.

Although the drug OK-432 can induce the release of gamma-interferon (IFN-gamma), the serum concentrations of IFN-gamma produced are very low. We studied the effects of combining OK-432 with alpha-interferon (IFN-alpha) on the endogenous production of IFN and the postoperative courses of patients with oral cavity cancers. Forty patients operated on for head and neck cancers were studied. Each patient was given an injection of OK-432 1 week after surgery. Between 10 and 14 days later, a combination of OK-432 and IFN-alpha was given to assess the effects of the concomitant use of IFN-alpha on IFN production. In 18 of the 30 patients given a large dose of IFN-alpha (3 or 5 X 10(6) IU/mg protein), IFN production induced by OK-432 was enhanced. A small dose of IFN-alpha (7 X 10(3) IU) did not enhance the action of OK-432. OK-432 also induced the release of both endogenous IFN-gamma and IFN-alpha, and the production of both types of IFN was enhanced by the concomitant administration of parenteral IFN-alpha. Next, 50 patients operated on for oral cavity cancers were given OK-432 or a combination of OK-432 and IFN-alpha for 4 months, and their postoperative courses were followed for 2-5 years. The clinical courses were better in the combined therapy group than in the group given OK-432 alone.

Biological Products↗
PubMed1986

Nodular fascitis of the mandible in a child.

Nodular fascitis is an unusual, tumor-like, proliferative, fibroblastic lesion with infiltrative properties. We describe a case of nodular fascitis appearing in the mandible of a 2-year-old boy. A brief review of the literature on this lesion arising from intra or perioral regions, and the importance of differential diagnosis for this lesions are discussed.

Child, Preschool↗
PubMed1986

Studies on 3-methoxy-4-hydroxyphenylglycol (MHPG) and 3,4-dihydroxyphenylglycol (DHPG) levels in human urine, plasma and cerebrospinal fluids, and their significance in studies of depression.

Both concentrations of total 3-methoxy-4-hydroxyphenylglycol (MHPG) and 3,4-dihydroxyphenylglycol (DHPG) in the human urine, plasma and CSF were determined with a high-pressure liquid chromatography with electrochemical detection in order to clarify the dynamic change in these noradrenaline metabolites. Three different biological fluids were collected simultaneously from 16 orthopedic patients who were regarded clinically as substitutes for normal subjects. In the urine, the MHPG concentrations were 1.67 +/- 0.65 micrograms/mg creatinine (mean +/- S.D.) and DHPG 0.39 microgram/mg creatinine +/- 0.21. The plasma levels were 21.16 ng/ml +/- 9.58 for MHPG, and 19.58 ng/ml +/- 8.13 for DHPG. The CSF levels of MHPG and DHPG were 24.08 ng/ml +/- 8.10 and 34.76 ng/ml +/- 11.46, respectively. The CSF levels of these metabolites were correlated significantly with those in the plasma (r = 0.852, p less than 0.001 for MHPG; r = 0.799, p less than 0.001 for DHPG), while no significant correlations were found between the urinary levels and either the plasma or CSF levels of these metabolites. In the urine, the MHPG levels were proportional to the DHPG levels, while the former were inversely proportional to the latter in the plasma or CSF. Neither the MHPG nor DHPG levels in the urine from depressed patients revealed to have any significant correlation with their clinical assessments using the Hamilton Rating Scale Score (HRS). The patients were treated with an antidepressant active selectively on the noradrenergic system, and no significant changes in urinary excretion of these metabolites were observed before and after the drug treatment. These findings suggest that in the case of psychiatric disorders such as depression, these compound levels in the plasma or CSF would provide more important information than those in the urine.

Adult↗