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H Wysocki

Publications and source records attributed to H Wysocki.

At least 73 records · Page 4Linked to original sources
PubMed1986

A new micromethod for the evaluation of Fc receptor expression on polymorphonuclear neutrophils.

A simple method for the evaluation of rosetting polymorphonuclear neutrophils is described. It uses small (200 microliter) quantities of heparinized whole blood and is simpler than the micromethod proposed by Buescher and Gallin. The high concentration of neutrophils and paucity of lymphocytes considerably simplify the evaluation of specimens and make the technique particularly useful in granulopenic states. There was a close correlation between results obtained using this technique and those from a standard method requiring the isolation of polymorphonuclear neutrophils.

Bronchitis↗
PubMed1985

Phagocytosis, bactericidal capacity, and superoxide anion (O2-) production by polymorphonuclear neutrophils from patients with diabetes mellitus.

Phagocytosis, bactericidal capacity and superoxide anion production of polymorphonuclear neutrophils (PMN) were estimated in 30 patients with well-controlled insulin-dependent diabetes (IDD) and in 50 patients with non insulin-dependent diabetes (NIDD). The estimations were additionally done in 20 elderly patients without glucose intolerance. The estimations of bactericidal capacity were performed in autologous-, zymosan activated-, inactivated- and control plasma. The phagocytosis of viable staphylococci was unchanged in all evaluated groups. The bactericidal capacity in all diabetic patients was significantly reduced. It was fully correctable in patients with IDD by suspension of cells in control or zymosan activated plasma. The improvement of PMN bactericidal capacity in patients with NIDD in similar conditions was less distinct. The superoxide anion production in patients with IDD was similar to values noticed in healthy persons. Whereas, the O2- production in patients with NIDD as well as in elderly patients were significantly reduced and correlated significantly with bactericidal capacity impairment. The possible mechanism of noticed disturbances were discussed.

Adolescent↗
PubMed1985

Intravascular degranulation of polymorphonuclear neutrophils during hemodialysis. The effects of pretreatment with colchicine.

Polymorphonuclear neutrophil (PMN) counts, plasma activity inducing PMN aggregation, augmenting PMN adherence and chemotactic activity were estimated during hemodialysis (HD) with cuprophane membranes. All these plasma activities are accepted as related to the presence of activated complement (C). The same estimations were made for hemodialyzed patients pretreated with colchicine. The results, especially comparison of plasma activities in blood entering and leaving the dialyzer, indicate that significant amounts of activated C components are generated within the patients vascular bed. The PMN entrapped in the pulmonary microcirculation that undergo subsequent degranulation are most probably the source of these C components. This concept was confirmed by the results for patients pretreated with colchicine, who had significantly less of the activities generated in the patients own circulation.

Adult↗
PubMed1985

The influence of thymus extract (TFX) on the phagocytosis and the bactericidal capacity of polymorphonuclear neutrophils from patients with insulin-dependent diabetes mellitus.

The influence of thymus extract (TFX) on phagocytosis and bactericidal capacity of polymorphonuclear neutrophils (PMN) from patient with insulin-dependent diabetes was studied. In previous observations PMN from these patients revealed the serum-related defect of bacterial killing. The preincubation of cells with standard dose of TFX significantly increased ingestion rate and recovered the bactericidal capacity to normal values. The augmentation of bactericidal capacity due to preincubation with TFX appeared to be dose-dependent and not related to other blood cells participation. The increase of bacterial killing was also evident, when TFX was applied after phagosome formation. The presented studies indicate that, among numerous peptides, thymus extracts contain those exerting their hormone-like activity on polymorphonuclear neutrophils. The observations may be of clinical importance.

Adult↗
PubMed1984

Plasma activity inducing polymorphonuclear neutrophils (PMN) aggregation, chemotactic plasma activity, and plasma activity augmenting PMN adherence in untreated patients with Hodgkin's disease. Possible relationship to disseminated intravascular complement activation.

The activity inducing PMN aggregation, the chemotactic activity, and the activity augmenting PMN adherence were estimated in plasma of 40 untreated patients with Hodgkin's disease. The first two activities were evident in all patients with advanced clinical stages (III and IV). It seems to be the result of disseminated intravascular complement activation due to the circulating immune complexes. The plasma activity augmenting PMN adherence was similarly noticed, except for 1, in patients with advanced stages of the disease, but only when the general symptoms were present. Our results, if confirmed, might be helpful in improving clinical staging of patients with Hodgkin's disease.

Cell Adhesion↗
PubMed1984

Phagocytosis and neutrophil bactericidal capacity in patients with uremia.

The phagocytic activity and bactericidal capacity of polymorphonuclear neutrophils (PMN) were evaluated in patients with advanced chronic renal failure. The studies were made in patients undergoing hemodialysis, maintenance peritoneal dialysis as well as in nondialysed patients. Evaluations were carried out by using of the recently described fluorochrome microassay which enabled these parameters to be estimate independently. The phagocytic activity was seriously diminished in nondialysed patients, whereas it was similar to controls in those hemodialysed and undergoing peritoneal dialysis patients. In all evaluated groups of patients the bactericidal capacity was significantly reduced. The lowest values could always be observed in nondialysed patients. The decrease of bactericidal capacity was significantly more evident in patients undergoing peritoneal dialysis as compared with those hemodialysed. The obtained results confirm some previous reports suggesting the impairment of PMN function in uremic patients. This results in their increased susceptibility to infection. They also reveal the existence of a close relationship between the extent of observed dysfunctions and the management applied.

Bacterial Infections↗
PubMed1983

Influence of hemodialysis on plasma chemotactic activity and the chemotactic responsiveness of polymorphonuclear neutrophils.

Thirty patients with chronic renal failure on maintenance hemodialysis (HD) were studied. Plasma chemotactic activity was estimated using the "under agarose" chemotaxis assay during the first 2 h of HD. It was found that in the fifth minute of HD, patients' plasma became chemotactic, reaching the maximum activity at the tenth minute. The chemotactic activity appearance correlated significantly with the decline in the number of the peripheral neutrophils. Patients' neutrophils, after a single passage through the cellophane coil of the dialyzer, revealed significant impairment of directed migration toward both complementary and bacterial chemoattractants. Moreover, the chemotactic properties of neutrophils obtained from dialyzed patients before HD were significantly lower than had been estimated in 15 nondialyzed patients with chronic renal failure. The results confirm HD-induced complement activation and might explain the mechanisms of the increased susceptibility of dialyzed patients to bacterial infections.

Adult↗
PubMed1982

Cardiopulmonary bypass-induced complement activation. Evidence for C5a participation.

Plasma activity inducing polymorphonuclear neutrophils (PMN) aggregation, augmenting PMN adherence, plasma chemotactic activity as well as peripheral PMN count were estimated in patients during cardiopulmonary bypass. In the course of the surgical procedure the chemotactic activity and the activity inducing PMN aggregation were detected in plasma. The top of these two activities was always followed by the significant fall of the peripheral PMN count. The increase of the activity augmenting PMN adherence was observed in a part of cases, but when noticed, always preceded the maximum of the remaining evaluated activities.

Adult↗
PubMed1981

Influence of lithium on neutrophil granulocyte distribution and turnover.

In 10 psychiatric patients marrow granulocyte reserve, marginal granulocyte pool, granulocyte mobilization into skin chamber, serum lysozyme activity was estimated before and ten days after the lithium treatment. In all patients the peripheral neutrophil count rose significantly. The marrow granulocyte reserve did not change markedly. Moreover, the marginal granulocyte pool as expressed in percentages diminished. Serum lysozyme activity, total leukocyte mobilization, and blood granulocyte clearance remained unchanged. However, the serum lysozyme activity index fell significantly. The in vitro addition of lithium to the suspension of granulocytes obtained from healthy persons reduce the granulocyte adherence especially in concentrations similar to those induced in treated patients. Our findings suggest that lithium in vivo may diminish the granulocyte adherence resulting in the prolonged intravascular half-life time of the cells. Thus, the cell accumulation in the vascular bed seems to be the main reason for lithium induced granulocytosis rather than the intensification of marrow granulocyte system proliferation.

Affective Disorders, Psychotic↗