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Biomedical subjects

H Wuthe

Publications and source records attributed to H Wuthe.

At least 19 recordsLinked to original sources
PubMed2001

Phenotypic and genetic characterization of NAD-dependent Pasteurellaceae from the respiratory tract of pigs and their possible pathogenetic importance.

Nicotinamide adenine dinucleotide (NAD)-dependent Pasteurellaceae other than Actinobacillus pleuropneumoniae and Haemophilus parasuis are frequently isolated from the respiratory tract of pigs. The taxonomic classification and relevance for pathogenicity of these bacteria deserves further attention. In the present study, 107 of these NAD-dependent isolates from the porcine respiratory tract, primarily from lungs with pathological changes, were investigated. On the basis of phenotypic criteria, such as haemolysis, urease, catalase, and indole formation as well as other fermentative activities, 50 of the isolates were assigned to Actinobacillus minor, 36 isolates to Actinobacillus porcinus and 21 isolates to Actinobacillus indolicus. However, many isolates among the three species showed fermentative activities differing from those of the respective type strain of the species. Serotyping on the basis of heat-stable polysaccharide antigens and 16 rDNA sequencing also revealed substantial heterogeneity within each of the three species although they clustered together in three distinct groups in the phylogenetic analysis. These three groups of NAD-dependent bacteria are different from, or in a borderline position, to the existing species or genera within the family Pasteurellaceae. A considerable number of isolates of these three groups were isolated in pure cultures from pneumonic lungs. Consequently, it will be necessary to critically review the opinion, that these NAD-dependent Pasteurellaceae are only "agents colonizing the mucosa". Further, taxonomic examinations of the strains within these three groups are indispensable to testing isolates for their virulence in gnotobiotic pigs.

Animals↗
PubMed1996

[Evaluating physical fitness after unilateral lung transplantation with ergometry and blood gas analysis].

7 patients aged 30-56 years with the perception of subjective increased fitness 4-22 months after single lung transplantation were tested for the objective benefit. Using a standardized ergometric test protocol cardiopulmonary parameters (ventilation, blood gases, gas exchange, blood pressure, ECG) were measured 5 males and 2 females. After transplantation in all patients the objective fitness was increased by 30-60 watts and the blood gases were markedly improved. The physical fitness of the subjects under study was probably limited by the reduced lung function of the native lung, the reduced mobility of the diaphragm in elderly patients and the impaired muscular strength and endurance.

Adult↗
PubMed1985

[Prognosis and indications of corticosteroid treatment of intrathoracic sarcoidosis--conclusions from a prospective study].

Preliminary results of a prospective clinical trial to evaluate the value of corticosteroid therapy of intrathoracic sarcoidosis had demonstrated that there were no differences between treated and untreated patients with regard to the clinical, radiological and functional outcome after several years. In one hundred and seventy two patients we could check the former results by identical tests after a longer follow-up period (mean 8,9 years) and by additional physiological assessments, i.e. Cstat, DLCO, DM, VC, which seem to be more significant for early detection of functional disorders in sarcoidosis. Again the findings showed no significant differences neither between patients treated with prednisolone for 12 or 6 months respectively nor when compared to the untreated group. In conclusion we do not treat patients with asymptomatic intrathoracic sarcoidosis and without extrapulmonary manifestations before the disease shows no regressive course within one year or when a progressive course is observed.

Adult↗
PubMed1985

[Factors influencing the diagnosis of lung function and our current reference values--results of the Pathophysiology of Respiration Society].

Reference values for lung function testing were determined of the spirometric volume and ventilation parameters VC, FEV1, RV (n = 3,750), bodyplethysmographic airway resistance and residual volume blood gases in rest (n = 5,779) and exercise (n = 117) considering the main influencing factors age, height, sex. The results were compared with international standards. Eleven laboratories of our country were engaged in examinations. The methods, the measuring conditions, the reference equations, and the valuation are described.

Adult↗
PubMed1984

[Lung function disorders in obesity].

For the pulmonary assessment of obesity or lung diseases in extremely obese persons the pathophysiological alterations and their consequences for assessment of pulmonary function are described. The review includes also own results.

Carbon Dioxide↗
PubMed1984

[Implementation and evaluation of the inhalation antigen test in the diagnosis of exogenous allergic alveolitis].

By the aid of antigen inhalation challenge test 16 patients with precipitating antibodies to bird antigens (pigeons, hens, budgerigars) and the supposed diagnosis of extrinsic allergic alveolitis (EAA) were examined. Criterions for the evaluation of the objective parameters (FEV1, FVC, Ros, body-temperature, number of leukocytes) were established. The combination of objective parameters and subjective symptoms leads to the evaluation of the challenge test. Different types of bronchial response (monophasic and dual-responses) were observed after inhalation of antigens. In 7 cases the diagnosis of EAA was proved to be true according to the challenge test results. If there are no typical alterations in chest-x-rays and no alterations of ventilatory function, the antigen inhalation challenge test is the method of choice in the diagnosis of EAA.

Adolescent↗
PubMed1984

[Experiences with oscilloresistometry in addition to spirometry in preoperative function diagnosis in the elderly].

A sample of 57 geriatric surgical patients were investigated with the aim of establishing which additional information the forced oscillation method yields for the assessment of an increased operation risk as compared with spirometry. In addition to the proved diagnosis of a restrictive ventilatory disturbance as causing a limitation of the maximum available respiratory volume, the measuring of the oscillatory resistance makes it possible in particular to prove and quantify the obstruction. Over and above this, the measuring of resistance fluctuation while breathing at rest makes it possible to differentiate a geronto-typical exobronchial component of the obstruction. Conclusions are drawn for the improved care of geriatric surgical patients.

Adenoma↗
PubMed1984

[Value of spirometric, pneumotachographic and oscilloresistometric studies in the functional diagnosis of obstructive respiratory tract diseases].

Tests were conducted on 90 smokers and a control group of 20 non-smokers using not only simple spirometry but also pneumotachography and the forced oscillation technique, including flow- and resistance-volume graphs. FEV1 was proved to be a sensitive screening test for early recognition of disfunction in the region of the small airways. 39,5% of the younger light smokers already showed signs of impairment to the breathing mechanics indicating exobronchial obstruction. The heavy, mostly older, smokers frequently showed complex disfunction, and in 55,3% the oscillatory resistance revealed quantifiable endobronchial obstruction. The oscillatory flow-resistance-increase-volume (as a percentage of vital capacity) proved the most sensitive small-airways-test during middle age, permitting significant distinction of smokers from non-smokers up to the age of 55.

Adult↗