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Biomedical subjects

H Wu

Publications and source records attributed to H Wu.

At least 505 records · Page 28Linked to original sources
PubMed1998

[Primary posterior chamber intraocular lens implantation of posterior capsular ruptur with cataract extracted by phacoemulsification].

OBJECTIVE: To evaluate the effect of primary posterior chamber intraocular lens (IOL) implantation of posterior capsular rupture (PCR) with cataract extracted by phacoemulsification. METHOD: Primary posterior chamber IOL implantation was performed on 29 cases (29 eyes) with PCR occurring in 184 cases (200 eyes) during cataract extraction by phacoemulsification. RESULTS: In-the-bag implantation was performed on 10 cases, and sulcus-fixed lens implantation on 19 cases. The post-operative uncorrected visual acuities in 17 cases (58.62%) were 1.0 or better and 0.5 - 0.9 in 10 cases (34.48%). The corrected visual acuities in 22 cases (75.86%) were 1.0 or better and 0.5 - 0.9 in 5 cases (17.24%) after 3 months of operation. The complications included corneal edema and damage of pupillary margin in the operation, and anterior vitreous inflammatory reaction after the operation. CONCLUSION: The posterior chamber IOL can be primarily implanted in cases with PCR and functional anterior or posterior capsular support after the anterior segment vitreous was cleaned.

Adult↗
PubMed1998

[The application of solid phase extraction for systematic toxicological analysis of abuse drugs].

A new column was developed in this research. It contains a proprietary bonded silica sorbent that exhibits mixed extraction mechanism. A single-column solid-phase extraction procedure was also developed for the screening of acidic, neutral and basic abuse drugs. The recovery of all 7 tested drugs exceeded 60%. The extraction mechanism for different abuse drugs on the new column was explored and was compared with on other columns. It is suggested that this column be effective in systematic toxicological analysis and better than other columns.

Antipsychotic Agents↗
PubMed1998

[Determination of chloroquine in human urine].

A GC method was established for qualitative and quantitative determination of chloroquine in human urine. The chloroquine is extracted from 2 ml urine with 2 x 2 ml cyclohexame: chloroform (8:2). The retention time of chlorquine is 9.44 min. The linear of quantitation is 0-50 micrograms/ml. The recovery of the procedure in 25 micrograms g/ml is 87.0%, CV = 7. 9% (n = 5). The limit of the determination is 200 ng/ml. The method can also be used in blood. A case is reported here. In that, we detected chloroquine in urine and blood. The quantitation of chloroquine in urine and blood is 0.475 mg/ml and 3.68 micrograms/ml, respectively. A metabolite of chloroquine-desethylchloroquine is also found in the urine.

Chloroquine↗
PubMed1998

[A new method for study of the effect of drugs on cancer cells--31PNMR with perfused cell system].

31P NMR was performed for the studies on the metabolic changes of endogenous phosphorus-containing molecules in KB and HCT-8 cells exposed to taxol at a concentration of 10(-6) mol.L-1. Using the perfusion method, the cells can be detected for a longer time by NMR, so as to give continuous spectra of the two cell-lines during the perfusion with and without the drug. The spectra showed that the levels of ATP peaks for both cells enhanced during the perfusion with the drug, but the change of the level is more prominent in KB cells than in HCT-8 cells. This shows that KB cells are more sensitive to taxol than HCT-8 cells. This is in coincident with the result in the cytotoxicity studies. However, the experiment using vincristine at the same concentrations demonstrated that the level of the ATP peak was not changed significantly.

Adenosine Triphosphate↗
PubMed1998

[Quantitative analysis on platelets cytosolic free calcium concentration and ulcerous tissue calcium in patients with recurrent aphthous ulceration].

To study the relationship between the abnormal behavior of calcium (Ca2+) and the pathogenesis of recurrent aphthous ulceration (RAU) by measuring quantitatively the concentration of platelets' cytosolic free calcium ions ([Ca2+]i) and tissue calcium (Wca2+), the patients with RAU (n = 50) and the healthy volunteers (n = 30) were chosen randomly. Then platelets [Ca2+]i was determined in calcium fluorescent indicator Fura-2/AM loaded washed platelets by spectrofluorophotometer and tissue Wca2+ was measured quantitatively by OCPC autoanalyzer method. The results showed that the concentration levels of platelets [Ca2+]i in patients with RAU in attack stage and attack-free stage were significantly higher than that of the control group (P < 0.01, P < 0.05), and the concentration level of the ulcerous tissue Wca2+ was also obviously higher than that of the normal control group (P < 0.001). There was a significantly positive correlation between the platelets [Ca2+]i and the ulcerous tissue Wca2+ (P < 0.01). The results suggested that the influx disorder and overload of Ca2+ might play an important role in the pathogenesis of RAU.

Adolescent↗
PubMed1998

[Researches on immunogenicity identification of recombinant Sj22.6(rSj22.6) kDa antigen gene of schistosoma japonicum].

AIM: To identify the immunogenicity and the potentiality of rSj22.6 as a candidate vaccine antigen for schistosomiasis japonica. METHODS: rSj22.6 was tested by Western blot with specific antibodies to identify its immunogenicity. The rSj22.6 protein was isolated and purified by SDS-PAGE and injected twice into rabbits to raise antirSj22.6 serum that was tested by Western blot to recognize the native protein of adult S. japonicum and SEA. C57 mice were immunized with rSj22.6 and challenged with S. japonicum cercariae to identify the protective immunity raised by rSj22.6. RESULTS: rSj22.6 could be recognized by specific antibodies and could stimulate the rabbits to produce high level of anti-rSj22.6 antibodies. Anti-rSj22.6 serum could recognize an adult native protein with the same molecular weight as rSj22.6. The preliminary immune-challenge test resulted in significant reduction in adult worm burden. CONCLUSION: rSj22.6 possesses immunogenicity in stimulating rabbits to produce high level of specific antibody and has protective role as a candidate vaccine antigen against S. japonicum infection.

Animals↗
PubMed1998

[Researches on the nucleotide sequencing of the recombinant Sj 22.6 kDa antigen gene of Schistosoma japonicum].

AIM: To sequence the cloned gene in pGSj24 and to identify the encoded protein. METHODS: The cloned gene in pGSj24 was digested from the recombinant plasmid by EcoRI, ligated into the M13mp19 vector and sequenced by automatic sequencer. The sequence was analyzed by Goldkey DNA and Protein Analytical Program and DNASIS Program. RESULTS: The pGSj24 cloned gene was demonstrated to be 840 bp containing one opened reading frame (ORF) with an initiation codon ATG at position 23 nt and a termination codon TAA at position 596 nt, encoding a protein with a molecular weight of 22.6 kDa. At the upstream and downstream of the ORF there were termination codons, so the encoded protein was unable to be larger. However, there was a termination codon TAA at position 11 nt, suggesting why the 22.6 kDa protein expressed separately. The nucleotide sequence of the pGSj24 cloned gene shared 95% identity with that of the corresponding part of S. japonicum 22.6 kDa antigen gene, and 99.7% identity in the encoding part. The deduced amino acid sequence analysis showed a sequence motif known as EF-Hand calcium binding domain, several endoplasmic reticulum targeting sequences and microbodies C-terminal targeting signals. The possible antigen determinants were predicted within the amino acid fragments of 29-32aa, 63-68aa and 87-101aa. CONCLUSIONS: The cloned gene in pGSj24 is the gene that encodes Sj22.6 kDa antigen.

Amino Acid Sequence↗
PubMed1998

[Screening and characterization of Schistosoma japonicum reinfection-related cDNA clones].

AIM: To obtain cDNA clones coding for S. japonicum (Sj) reinfection-related specific immunologic molecules for vaccination or diagnosis. METHODS: An adult S. japonicum cDNA library, of which the cloning efficiency is 3.13 x 10(6), with directional inserts in the vector lambda gt11, was screened with pooled serum from 43 reinfected human cases in a schistosomiasis-endemic area with high level of Sj specific-lgG4 antibodies. Primers located several bases away from the insert position on the lambda gt11 arm were used to amplify the insert fragments by standard PCR. SILVER SEQUENCE (PCR-silver) DNA sequencing system was then used to sequence selected positive clones with relatively large inserts and homology comparison with GenBank database was also carried out. RESULTS AND CONCLUSION: Eleven phage plaques were defined as positive out of the 8.6 x 10(4) phage plaques immunoscreened by human specific lgG4 antibody. GenBank database retrieval showed that 4 new Sj gene clones including Sj reinfection-related specific protein-encoding gene and Sj mitochondrial protein-coding gene have been obtained.

Adolescent↗
PubMed1998

[Effects of different amount of ginger juice on prepared products of Rhizoma Pinelliae].

Rhizoma Pinelliae were diged in Ginger juice of different propertion, the total alkaloid contents of these products were all higher than the raw Rhizoma Pinelliae, and related to the actions of anti-tumous cell. The contents of beta-sitosterol of these products were related to the amount of Ginger juice which is added in Pinelliae Rhizoma, but only the content of beta-sitosterol of the Pinelliae product which is prepared by same amount of Ginger juice was slight higher than the raw Rhizoma Pinelliae. The stimulations of five kinds of products on mice have no obvious relation to the amounts of Ginger juice which were added in Rhizoma Pinelliae, but the stimulation of the Pinellia product which was prepared by same amount of Ginger juice was the lowest of all products on mice. Dependent on above experiment results, we suggest that Rhizoma Pinelliae processed by Ginger juice should be prepared by same amount of Ginger juice with Rhizoma Pinelliae.

Alkaloids↗
PubMed1998

[Supercritical-CO2 fluid extraction of Allium stativum oils].

Effective constituents from bulb of Allium stativum were extracted by supercritical-CO2 fluid. These constituents were analyzed by GC-MS. The results showed that oils from SFE-CO2 contained 12 components, two of them were first obtained from the plant.

Allium↗
PubMed1998

[The experiment research of ginger detoxification to Rhizoma Pinelliae].

The experiment results have shown that Ginger can abate the stimulation of Phizoma Pinelliae on abdominal cavity of mice, reduce the torsion incidence rate of mice because of pain which is resulted to i.p. powder suspension of raw Rhizoma pinelliae. We also observed that Ginger can obviously inhibit the increase of capillary permeatility of abdominal cavity of mice, reduce the PGE2 content of inflammatory foot tissue of mice which is due to inject juice of fresh Pinellia Rhizoma. In addition, Ginger can increase the PGE2 content of mice gastric solution, antegonise the decrease of PGE2 content which is come from administration of raw Pinellia Rhizoma and protect the gastric mucous. Above mentioned results suggested that Ginger have detoxification in vivo. to toxin of Pinellia Rhizoma.

Abdominal Cavity↗
PubMed1998

[Study on the supercritical-CO2 fluid extraction of Prunus mandshurica oils].

The paper reported technological study on extracting Prunus mandshurica oils by supercritical-CO2 fluid, mainly researched the influence of pressure, temperature, time and flow rate of CO2 on the oil yield, determined optimum technology of extracting the oils, compared the oils from SFE-CO2 and traditional technology.

Carbon Dioxide↗
PubMed1998

[Determination of the content of serum calcium with methylthymol blue as chromogenic reagent].

It is reported in this paper that calcium can be determined with MTB as chromogenic reagent by isoabsorption dual-wavelength elimination. The measuring wavelength and referential wavelength were 613.7 and 590.0nm, respectively. No masking reagent was necessary in the analysis. The average recovery and RSD of calcium are 98.98% and 0.81%, respectively. Compared with titration, this method is rapid, simple and precise.

Absorption↗
PubMed1998

Prenatal ethanol exposure alters the modulation of the gamma-aminobutyric acidA1 receptor-gated chloride ion channel in adult rat offspring.

We examined the effect of prenatal ethanol exposure on gamma-aminobutyric acid (GABA)-stimulated 36Cl- flux. Sprague-Dawley rat dams were fed either a liquid diet containing 5% ethanol, pair-fed an isocalorically equivalent 0% ethanol diet or rat chow ad libitum throughout gestation. Membrane vesicles were prepared from medial frontal cortex, cerebellum and hippocampal formation of adult offspring in each diet group. GABA-stimulated 36Cl- flux was not significantly affected by prenatal ethanol exposure in any of the three brain regions examined. Positive allosteric modulation of GABA-stimulated 36Cl- flux by flunitrazepam or alphaxalone, as well as negative modulation by FG-7142 or pregnenolone, were all diminished in medial frontal cortex of 5% ethanol diet offspring compared with both ad libitum and pair-fed control groups. In cerebellum, prenatal ethanol exposure attenuated the modulatory effects of both benzodiazepines, but did not affect neurosteroid modulation. In hippocampus, prenatal ethanol exposure enhanced the effects of flunitrazepam and alphaxalone, whereas negative modulatory effects were either decreased (FG-7142) or unchanged (pregnenolone). These results indicate that moderate ethanol consumption during gestation can produce long-lasting alterations in neuromodulatory influences on GABAA receptor-mediated inhibitory neurotransmission in adult offspring. In hippocampal formation, the heightened sensitivity to positive modulatory influences may contribute to synaptic plasticity deficits in fetal ethanol-exposed rat offspring. We speculate that these prenatal ethanol-induced changes may be either a consequence of differential GABAA receptor subunit expression or receptor uncoupling in different brain regions. Furthermore, offspring exposed to ethanol in utero may display differential sensitivities to benzodiazepines and possibly other centrally active therapeutic agents.

Animals↗
PubMed1998

Transmission and natural regulation of infection with Ascaris lumbricoides in a rural community in China.

A longitudinal study on a cohort of 215 people who remained untreated from June 1993 to June 1994 was carried out using fecal egg counts to examine the course of transmission and aspects of the natural regulation of the infection with Ascaris lumbricoides. The results indicate that the parasite population remained relatively stable during the years since overall prevalence remained steady above 60%. About 40% of the people in the cohort retained their infection intensity status throughout the year, in terms of light, medium, and heavy infections. The regulation of infection in the population could be attributed to the tendency for the loss of worms from people with initial light infections and the movement to medium infection intensity status of people with both light and heavy infections. Compared with adults, children showed a stronger tendency of retaining initial medium infections but weaker tendencies for keeping initial light infections and clearing themselves of infection. Predisposition to light and medium infections was detected in people who had experienced an uninfected period of 4-12 mo before reinfection was established.

Adolescent↗
PubMed1998

Morphometric and electron-microscopic analyses of the effects of gonadotropin-releasing hormone agonists on uterine leiomyomas.

OBJECTIVE: Reports on the histologic effects of gonadotropin-releasing hormone agonists on uterine leiomyomas provide conflicting results. Most previous studies used qualitative methods of analysis. Using quantitative and semiquantitative stereologic methods of analysis, we assessed volume density of hyalinized areas, cell density, nuclear volume, and cytoplasmic cross-sectional areas of smooth muscle cells in histologic sections and also measured diameters of collagen fibrils in electron micrographs of uterine leiomyomas. DESIGN: Thirty leiomyomas from patients treated with gonadotropin-releasing hormone agonists (10 different patient samples), age-matched control patients (10 different patient samples), and postmenopausal women (10 different patient samples) were used. Hyalinization was assessed using a microscope with a projection head and affixed morphometric grid. Cell size and density were evaluated using a video-based, computerized system attached to the microscope, for which morphometric ad hoc programs were written. Diameters of collagen fibrils were measured from electron micrographs. SETTING: The study was conducted in the Department of Pathology, Mount Sinai Medical Center, New York, NY. PATIENTS: A total of 30 patient samples were studied, with three groups comprising 10 samples each, including patients treated with gonadotropin-releasing hormone agonists, age-matched control patients, and postmenopausal women. RESULTS: Myomas from patients treated with gonadotropin-releasing hormone agonists exhibited more hyalinization, greater cell density, slightly smaller cell sizes, and larger collagen fibrils than those of age-matched control patients and postmenopausal women. CONCLUSIONS: Shrinkage after treatment with gonadotropin-releasing hormone agonists is attributed to smaller cell size and increased collagenization in myomas.

Adult↗
PubMed1998

[The influence of several factors on automated DNA sequencing].

OBJECTIVE: To investigate the influence of several factors on isothermal automated DNA sequencing. METHODS: The effects of DNA purity and DNA concentration, as well as the quality of water used during sequencing on automated DNA sequencing results were retrospectively analyzed. RESULTS: When the DNA was not pure enough, the nucleotide curve showed lower ratio of signal to noise, or compression would occur, leading to difficulty in correctly judging the nucleotides; the readable length of nucleotides was shorter when low concentration of DNA was used; when the quality of water used in sequencing reaction and polyacrylamide gel solution was not high enough, the peaks of nucleotides became wider and flatter, or double peaks would occur, resulting in unreadable sequences. CONCLUSION: DNA purity and concentration as well as quality of water used in sequencing reaction and polyacrylamide gel solution had apparent influence on isothermal automated DNA sequencing. This suggests that high purity of DNA and high quality of water should be used in isothermal automated DNA sequencing; if longer DNA sequence needs to be obtained, DNA concentration should not be too low.

DNA↗