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Biomedical subjects

H Wolff

Publications and source records attributed to H Wolff.

At least 235 records · Page 13Linked to original sources
PubMed1981

[Electron microscopic findings during hypothermic storage. Preservation of the liver. I. (author's transl)].

Porcine livers were preserved by simple hypothermic storage for 6 h using plasma-protein-fraction 5%, albumin 5%, Collins C2, and Ringer-lactate. Electron-microscopic alterations primarily consist of aggregations of nuclear chromatin, marginal chromatin condensation, swelling and destruction of cristae mitochondriales, swelling and degranulation of the endoplasmic reticulum. These signs of cell damage are of varying intensity depending on the different preservation methods. The best condition of hepatocytes on the whole is guaranteed by using Collins C2-solution.

Animals↗
PubMed1981

[Electron microscopic findings during hypothermic storage preservation of the liver. II. (author's transl)].

Porcine livers were preserved by simple hypothermic storage for 6 h using plasma-protein-fraction 5%, albumin 5%, Collins C2, and Ringer-lactate followed by a morphometric evaluation of the electron-microscopic changes. The morphometric parameters reflecting swelling of mitochondriae showed the most pronounced changes and were suited best for reproduction. The best result was achieved by using Collins C2-storage. The endoplasmic reticulum was found to be only slightly swollen in all preservation solutions investigated here, at the same time a degranulation of granular endoplasmic reticulum could be demonstrated. The increase in volume of lysosomes per unit-volume was not significant.

Animals↗
PubMed1981

[Progress in transplantation of the pancreas (author's transl)].

In the field of pancreas transplantation great progress has been made in recent years. While the transplantation of the whole organ has lost importance, success could be reached with the transplantation of isolated islets of Langerhans and with the segmental pancreas transplantation in experiments and in clinical practice. The present-day research is focused particularly on influencing the antigenicity of the endocrine pancreas tissue and on methodical problems of segmental pancreas transplantation.

Animals↗
PubMed1981

[Transplantation register for pancreas and island of Langerhans].

A new registry for human pancreas and islet transplantation has been established (D. E. R. Sutherland). Between 17 December 1966 and 31 December 1980, 108 pancreas transplantations were performed. Six patients had grafts that functioned for more than 12 months. In most of the 68 islet allotransplants performed, little or no evidence of islet function has been obtained. Three diabetic patients were dependent on insulin for 2.5 to 8 months after the operation. One of these, however, still has a functioning graft. Autotransplants of dispersed pancreatic islet tissue have been performed on 59 patients after total or partial pancreatectomy for benign disease. The 28 patients who had 90% pancreatectomies did not require insulin. Information from the registry should be helpful to the various transplant teams attempting clinical application of these procedures.

Diabetes Mellitus↗
PubMed1981

[Glycolysis during hypothermic storage preservation of the liver].

Porcine livers were preserved for 6 hours by simple hypothermic storage using plasma-protein-fraction, albumin, Collins C2, Ringer solution, and Ring-trickle-perfusion. Following glycolytic metabolites were examined: G6P, F6P, FDP, GAP, 2PG, PEP, pyruvate, and lactate. An alteration of glycolytic metabolite-profile by the different preservation methods was not seen. There is a limitation of glycolysis by the pyruvate-kinase-reaction. Glycolysis decreases depending from preservation time. An artificial increasing of glycogen or rate of glycolysis before hepatectomy seems to be without any effect.

Animals↗
PubMed1981

[Behavior of adenine nucleotides ATP, ADP and AMP in liver preservation].

Porcine livers were preserved by simple hypothermic storage using plasma-protein-fraction (PPF), albumin- (A), Collins C2- (C), Ringer-solution or Ringer trickle perfusion. Adenine nucleotides were examined. The highest ATP-concentration after 6 hours was achieved by PPF. The sum of adenine nucleotides differed only slightly in PPF-, A-, or C-preservation and was higher than after R- or RT-preservation. The terminal concentration solution is given. An artificial increasing in AdN-content before hepatectomy is probably without any effect.

Adenine Nucleotides↗
PubMed1980

[Surgery of the extrahepatic bile ducts].

The surgery of the bile-deviating system possesses approved methods which guarantee a successful proceeding. They are supplemented by endoscopic interventions which in the development will contribute to the further improvement of the treatment. Difficulties appear in the surgical therapy of the inflammatory and malignant obstructions of the biliferous duct. In this case the early diagnosis as well as an improved technical approach is indicated. It is referred to the possibilities of the formation of a permanent and functional deviation of bile. The treatment of malignant processes still too often and too early is regarded with an unnecessary resignation and thus the patients are denied proved chances of survival. The possibilities are entered to procede in this case successfully in operation.

Bile Duct Neoplasms↗
PubMed1980

[Immunoglobulins after experimental liver transplantation in pig].

During a period of 14 days after porcine liver transplantation immunoglobulins IgA, IgG, and IgM were investigated. IgA and IgG were slightly elevated at the end of investigation. The increase in IgM was higher and significant. This rise in immunoglobulin-level is thought to be a result of immunisation by parenchymatous insults after liver transplantation.

Animals↗
PubMed1979

[E-rosette formation after liver transplantation (author's transl)].

In 1 patient with orthotopic liver transplantation E-rosette formation was investigated during a period of 16 months. The patient showed an average rate of rosette formation comparable to this of controls. Increased or decreased rates were connected to crises--cholangitis and rejection--in clinical currency.

Adult↗
PubMed1979

[Surgical management of reno-vascular hypertony (author's transl)].

Only in 2/3 out of a total of 33 patients suffering from renal artery stenosis surgery was indicated. Thrombendarterectomy and saphenous patch are the method of choice for reconstructive procedures. In 87% of successful reconstructions the functional result was satisfactory. The follow-up gave evidence of change to the worse due to the progress of the underlying disease.

Adult↗
PubMed1979

[The diffusion of cefazedone into heart muscle, prostatic and skin tissue and into bile].

The diffusion of cefazedone into human heart muscle, prostatic and skin tissue as well as bile fluid was investigated. 40 to 80 min after a single injection of 100 mg/kg (n = 14) the concentration in the heart muscle was between 10.8 and 85.5 micrograms/g. The respective serum levels were between 117 and 168.1 micrograms/ml. The single i.v. injection of 2 g cefazedone resulted within 30 min in a mean concentration of 34.63 +/- 9.75 micrograms/g in the prostatic tissue and in serum levels of 139.07 +/- 39.68 micrograms/ml (n = 14). In 5 patients additional values were estimated after 60 min. At this time the antibiotic concentrations were 24.92 +/- 1.31 micrograms/g in the tissue, with simultaneous serum levels of 87.25 +/- 20.86 micrograms/ml. 1 h after a 500 mg i.v. dose, concentrations in bile taken from T-tube were between 71.4 and 210 micrograms/ml. After 2 h there was a mean level of 83.2 micrograms/ml which was significantly above the serum concentrations at the same time (1 h = 35.25 +/- 7.17; and 2 h = 20.5 micrograms/ml). The bile concentration of 2 patients taken 5 h after cefazedone injection was 4.95 and 11.6 micrograms/ml. The cefazedone concentrations in the skin were estimated mainly in biopsies from granulating leg ulcer tissues. The mean concentrations in 4 cases were 120 +/- 28.7 micrograms/g 3 h after i.v. injection of 2 g cefazedone. The simultaneous serum levels were between 14.85 and 68.2 micrograms/ml, in one patient with extreme venous stasis the tissue concentration was only 8.1 micrograms/g. Cefazedone should be regarded as an antibiotic with excellent penetration into tissues.

Adolescent↗
PubMed1978

[Pacemaker and seat belt (author's transl)].

Although seat-belt-induced traffic accident injuries to wearers of pacemakers are rare, about 50,000 pacemaker patients have to "buckle up." A study of 450 pacemaker patients indicated that, as in the general population about 50% use their seat belts. Fifty percent of the patients felt uncomfortable or showed local damage of the area overlying the pacemaker. Studies with dummies revealed a 7-cm upward dislocation of the pacemaker caused by the negative acceleration and by the seat belt itself during the crash. This could be prevented by an external cap protecting the pacemaker.

Accidents, Traffic↗
PubMed1978

Interrelationships among arenaviruses measured by indirect immunofluorescence.

An evaluation of reciprocal indirect immunofluorescent antibody titers for at least one representative of each known arenavirus serotype leads to the conclusion that the Old World aranaviruses are more closely related to each other than to other members of the group. These relationships are particularly evident in evaluations of human convalescent sera. New World arenaviruses cross-react strongly with each other in most instances but only weakly with with Old World arenaviruses.

Animals↗
PubMed1978

[Liver transplantation in animal experiments].

Liver transplantation has extraordinarily complicated technical, biochemical, and pathophysiological aspect. The present study on experimental liver transplantation is a survey on essential parts of this vast problem. Particularly in the fields of biochemistry and immunology, our knowledges must be extended before an improvement of the results can be achieved. Results from experiments of the authors themselves agree with experiences, complications, and survivals after hepatic transplantation reported in literature.

Anesthesia↗
PubMed1977

[beta-glucuronidase activity in human tumors of the lung and of the mammary gland (author's transl)].

The intratumoral activity of beta-Glucuronidase (total and free) was determined in surgical specimens of malignant tumors of the lung (N equal to 20) and of the breast (N equal to 15) from patients. A free beta-Glucuronidase-activity from about 90 mU per g tissue was found but there was no difference between both tumor types, and the beta-Glucuronidase-activity, as it seems, is not dependent on histological characteristics of the tumors. The findings are discussed under the view of the key function of the lysosomal enzymes in the concept of the "Cancer-Multistep-Therapy" by M. von Ardenne.

Breast Neoplasms↗