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Biomedical subjects

H Wilken

Publications and source records attributed to H Wilken.

At least 37 records · Page 2Linked to original sources
PubMed1989

[Applicability of the subrenal capsule assay (SRCA) in primary breast carcinoma].

We have examined the application of the subrenal capsule assay (SRCA) by use of normal immunocompetent (NIC) mice for chemosensitivity testing of primary breast cancers. In 18 out of 20 carcinomas an evaluation was possible. AB mice (10 breast cancers) and B6D2-F1 mice (10 breast cancers) were taken as recipients. Measurement of the size and the histological examination of all transplants were obligatory performed before and after finishing the assay on day 6. By use of AB mice as well as B6D2 mice in each group in 6 out of 9 evaluable cancers occurred a significant growth of the transplanted tissues after 6 days (p less than 0.05). It could be demonstrated by the histological examination that the enlargement was a consequence of the immunological reaction of the NIC mouse to the xenogeneic material. In most cases (76 of 97 used mice) the transplants consisted of inflammation cells only. Vital tumor cells were seen only in few cases (21 of 97 used mice). It is concluded, that the SRCA is not suitable for chemosensitivity testing of human breast cancer, if NIC mice as recipients are used.

Animals↗
PubMed1989

[Effectiveness of perioperative preventive use of antibiotics with metronidazole or doxycycline in vaginal hysterectomy].

To prove the effectiveness of perioperative antibiotics prophylaxis (PABP) in prevention of postoperative infections after vaginal hysterectomy the efficacy of rectale Metronidazole application (5 times 500 mg; n = 192) was compared with that of Doxycyclin (2 times 200 mg intravenously; n = 116). The infectious morbidity after both prophylactic antibiotic regimes was compared to that of an untreated group (n = 186). In spite of additionally colporrhapies the feverish standard morbidity without prophylaxis was 13.4%, after Metronidazole application 13.5% and after Doxycyclin 2.6% (p less than 0.05). Simultaneously the rate of necessary antibiotic treatment decreased significantly. Pelvic infections complicated 16.7% of vaginal hysterectomies having no prophylaxis, but only 4.3% after Doxycyclin medication (p less than 0.05). Postoperative urinary tract infections (UTI)--mostly asymptomatic bacteriuria--were not influenced by any PABP. Whereas UTI after bladder catheter duration shorter than two days occurred in 26.0% (without PABP), 29.9% (Metronidazole) respectively 18.2% (Doxycyclin; p greater than 0.05), the rates after a longer stay were 69.6%, 76.8% and 65.7%. It is concluded, that the febrile standard morbidity and pelvic infections were reduced as well as the rate of necessary antibiotics therapies by a prophylactic application of Doxycyclin, but not with Metronidazole. The incidence of postoperative UTI was not reduced by prophylaxis.

Administration, Rectal↗
PubMed1989

[Effectiveness of perioperative preventive use of antibiotics with ampicillin/gentamycin or cefotiam in abdominal cesarean section].

To prove the effectiveness of perioperative antibiotics prophylaxis (pabp) in prevention of postoperative infections after caesarean section the efficiency of a Ampicillin/Gentamycin combination was compared with that of Cefotiam (Halospor). The infectious morbidity after both prophylactic antibiotic regimes was compared to that of an untreated sectioned group. The feverish standard morbidity (fsm) of all caesarean sections (between 1. 7. 1986-30. 6. 1988; n = 354) decreased by selective pabp from 25.3% to 16.7%. Compared with untreated patients (no infectional risk; fsm: 22.4%) the infectional morbidity after caesarean section was reduced by Ampicillin/Gentamycin (fsm: 10.0%; p less than 0.05) and also by Halospor (fsm: 13.7; p greater than 0.05). Uterine (14.5%) and urinary tract infections (6.6%) were the most frequent causes of fever during puerperium. After pabp the frequency of this causes decreased evidently. The rate of postoperative antibiotics therapies went down from 26.3% without pabp to 13.3% (p less than 0.05) with Ampicillin/Gentamycin respectively 13.7% (p less than 0.05) with Cefotiam. It is concluded, that the feverish standard morbidity was reduced by a selective papb both with Ampicillin/Gentamycin and Halospor. As a consequence of our results we are performing the pabp with a single dose of 2 g Halospor combined with 0.5 g Metronidazol in all sections generally.

Ampicillin↗
PubMed1989

[Evaluation of the subrenal capsule assay (SRCA) in breast cancer using immunocompetent (NIC) mice].

UNLABELLED: To assess the value of subrenal capsule assay (SRCA) for predictive chemo-sensitivity testing in breast cancer 24 primary tumours were examined in normal immunocompetent (NIC; AB- and B6D2-F1-) mice. In 6 cancers the effect of 0.25 mg Prednisolone mous X day (applicated with drinking water) was compared to the control group. Independent of the mice-species a significant increase of the grafts size occurred in 14 of 20 evaluable cancers (p less than 0.05). Histologically the increase of tumour size was caused by infiltrated host cells, only in 22 of 107 (20.6%) grafts vitale tumour cells were detectable. After immunosuppression the increase of the grafts size was significant lower, than in the no treated control group (p less than 0.05). In groups treated with Prednisolone only a thin inflammatory host cell infiltration was observed, but vital tumour cells were only seen in 3 of 29 (10.3%) grafts. CONCLUSION: Using normal immunocompetent mice subrenal capsule assay is not suited for predicting responsiveness of breast cancers to cytostatic drugs.

Animals↗
PubMed1989

[Success rates in in vitro fertilization--a mathematical problem? Comments on the article by M. M. Seibel in Obstet. Gynecol. 72 (1988) 265].

In vitro fertilization, GIFT and related methods have given new perspectives for the treatment of sterility and are applied worldwide. Despite an increasing application of these methods success rates are still relatively low. It may be difficult for sterility patients and their gynecologists to get a clear imagination about the success rates of an IVF-program. This was mentioned in the "Green Journal" by M.M. Seibel, the article of whom has been taken as a basis for some thoughts about the problem. Unclear statements and different bases for reports of success rates may lead to unrealistic expectations concerning the chance for a successful treatment by IVF. Therefore, in discussion with patients only comprehensible figures should be mentioned, e.g. a rate involving numbers of term pregnancies and oocyte retrievals. Taking this as a crucial point the patient and her partner should be informed about their chances depending on their individual findings and the facilities of the sterility centre.

Adult↗
PubMed1988

[Initial experiences in the use of pulsatile Gn-RH administration for ovulation induction].

In 9 patients the pulsatile administration of Gn-RH for the induction of ovulation was given in 13 treatments, we used the minipump Zyklomat of the firm Ferring and a Gn-RH pump from ZID Karlsburg. The administration was carried out for 10 to 18 days with dosages from 2.5 to 20 micrograms Gn-RH per pulse. 9 patients did ovulate and 5 patients became pregnant.

Adult↗
PubMed1988

[Current problems of blood group incompatibility in pregnancy].

The frequency of hemolytic disease of fetus and newborn has decreased since introduction of Rh-prophylaxis. In spite of that till now hemolytic disease caused by anti-D antibodies occur. It is probably caused by immunization already during pregnancy. Therefore it seems to be useful to give anti-D-Immunoglobulin (200 micrograms = 1000 IE) in 28-30th week of pregnancy in all d-women with D-husband. This seems especially necessary in pregnancy complications as hemorrhages in 3rd trimester, multiple pregnancies, trauma, and external version of breech. Rh-prophylaxis post partum is to perform in usual manner. As done before 2 time screening for irregular antibodies in pregnancy is necessary. Amniocentesis is often required in presence of antibodies against D, C, c, E, e, K, Fya, Fyb, S, s, U, Jka, Jkb and Dia. When hemolytic disease is confirmed, intrauterine transfusion or preterm delivery has to be performed. Immunization have increasing importance against erythrocyte factors other than D. It should give attention to it in transfusion practice during pregnancy.

Antibodies, Anti-Idiotypic↗
PubMed1988

[Report of experiences in 20 years use of intrauterine fetal transfusion in severe Rh-induced hemolytic disease of newborn].

136 intrauterine transfusions in 90 fetuses between the 25th and 34th gestational week with severe hemolytic disease because of Rh-incompatibility have been performed after a prenatal step by step diagnostic in the years 1967 till 1986. The reason of the immunization was mistransfusion or omitted immune prophylaxis in 25 cases. 25 from 67 live born children died. There were 23 still births. 34 from 43 fetuses without hydrops survived, but only 8 from 47 fetuses with hydrops. The survival rate over all was 46.7 per cent.

Blood Transfusion, Intrauterine↗
PubMed1987

[Dehydroepiandrosterone and other endocrine parameters in breast cancer].

A short review is given about some of the data reported in the literature on dehydroepiandrosterone and its derivatives, delta 5-androstenediol (hermaphrodiol) and sexual steroid binding globulin (SHBG) in breast cancer patients. The reported results lead to hypothesis that a decreased SHBG binding activity may be found in these patients.

Androstenediol↗
PubMed1987

[Regulation of the immunoglobulin content of the mucous membranes of the uterus: effect of estradiol on immunoglobulin A content].

A was used for the animal experimental investigations standard castration mouse model. Vaginal smears indicated no cyclic changes 14 days after the removal of the ovaries. 12.5 micrograms estradiol benzoate have been injected intramuscularly in this time. 3 days later uterus lavage fluids and tissue homogenates were used for the IgA-test, single radial immunodiffusion. The standard was prepared from mouse colostrum because S-IgA is the dominant IgA in secretions. The results were expressed as U/l selected arbitrary. Our findings of significant increased IgA concentrations in uterus lavage fluid after estradiol stimulation and, in contrary, no changed IgA concentrations in homogenates support the hypothesis, that the secretory component synthesis in the epithelial cells of the uterus is controlled by estrogens.

Animals↗
PubMed1987

[Individual therapy of breast cancer].

2 remarkable trends in the treatment of breast cancer are to be observed: The increasing use of breast preserving procedures and the recognition of quality of life of the breast cancer patient. The decision about treatment regime depends both on the stage of the breast cancer and on the wishes of the patient and therefore it is individualized. The management shall be performed by the principles of "informed consent".

Breast Neoplasms↗
PubMed1987

[Breast-saving operations in breast cancer].

Breast preserving surgery or conservative surgery consists at present of a combination of a reduced operation--tumorectomy or wedge resection or subcutaneous mastectomy--with adequate radiotherapy whereby a doses of 40-50 Gy is used with or without an additional booster dose of 10 Gy. Recent studies of 5-year- and 10-year-survival rates after conservative management have been shown to be identical with radical operations (Rotter-Halsted, Patey-Auchincloss). In the Departments of Gynecology and Obstetrics of the Universities of Münster and Rostock the partial bilateral subcutaneous mastectomy with lymphadenectomy and irradiation is the preferred method of conservative management for breast cancer. Indications and technique are discussed.

Breast Neoplasms↗
PubMed1986

Is priming an unripe cervix with PGF2 alpha effective?

A report is given about induction of labour in 1050 women using different methods to improve cervical condition before induction in 151 women. Administration of oestradiol intramuscularly had a better effect than the locally administered PGF2 alpha.

Cervix Uteri↗
PubMed1986

[New aspects in improved management of labor].

The increase of obstetric operating frequency observed in the last years is to attribute to a better recognition and valuation of maternal and fetal risk and on the other hand to a false interpretation of cardiotocography. With respect to induction and management of labour from a lot of partly controversial opinions is the following to conclude for the practice: Utilisation of all diagnostic possibilities including fetometry, pelvimetry, and cervical ripeness. Therapeutic arrangement should include cervical ripening, mobilisation of patient and labour timing depending on internal tocometry. The operative delivery is to consider as an ultima ratio. Labour management can be optimated by means of a clinic-specific partogram considering the active phase. Every indicated induction of labour demands internal tocometry. The pharmacological control of uterine activity must be used differentially. It must be waited for, if the quantification of labour activity improves the obstetrical-perinatological parameters.

Cesarean Section↗