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Biomedical subjects

H Werner

Publications and source records attributed to H Werner.

At least 325 records · Page 18Linked to original sources
PubMed1975

[Susceptibility to tetracycline and doxycycline of 80 strains of Bacteroides fragilis, B, thetaiotaomicron, and B. vulgatus mostly isolated in 1973/74 (author's transl)].

Recent studies by several authors suggested that Bacteroides fragilis and related species are becoming more resistant to tetracyclines. Therefore, the minimal inhibitory concentrations of tetracycline and doxycycline were determined by broth dilution tests for a total of 80 Bacteroides strains (56 B. fragilis, 15 B. thetaiotaomicron, 9 B. vulgatus), 70 of which had been isolated from clinical specimens during 1973/74. Using initial concentrations of 1 x 10(8) organisms per mo of thioglycolate broth the MIC were determined after 5 hr of incubation. Thus the influence of thermal inactivation of tetracycline compounds on the results obtained was minimized. Tetracycline at less than 0.00075 through 12.5 mug/ml was active against 79 strains. The MIC of one strain, a member of B. fragilis, was 50 mug tetracycline/ml. At 1.56 mug doxycycline/ml the cumulative percentage of sensitive strains was 97,5. The MIC of only 2 Bacteroides cultures was 3.12 mug doxycycline/ml. Taking account of the relatively high initial concentrations of test organisms, the results obtained may be estimated at the quotient of 1/4 as representative of the usual inoculum of 10(6) organisms/ml. Accordingly, 79 and 80 strains, respectively, may be considered to be sensitive to tetracycline and doxycycline in clinically achievable levels.

Bacteroides↗
PubMed1975

[Malignant neoplasms of colon and rectum in Berlin, capital of the G.D.R., 1960--1969 (author's transl)].

The situation of detection and treatment of colonic and rectal cancer in the capital of the G.D.R. Berlin is described for the period 1960--1969 and analyzed in comparison to other periods and regions. This study is based on the regional cancer registry and therefore free of the selection bias in clinical statistics. In the ten year period, 3855 new cases were registered (colon 2158, rectum 1697). The proportion of old patients is high, 66 percent were 65 years and older, 28% 75 and more years old. About 41% of patients seek medical advice within four weeks after first symptoms and about 60 percent of cancers were treated within one month after first consultation. The locaized stages I and II amounted to 46 percent of cases. There was only loose relation between duration of history and stage of disease. In nearly 39% of cases radical operation was performed and 27% were not treated by surgery. 5-year survival rates of colonic and rectal cancer in the period 1960--1964 amounted to 12% for all stages irrespeitive of method of treatment and 31% for patients after radical surgery. Survival rates depend on stage, on mode of treatment and on age. In the period from 1955 to 1973, proportion of old patients increased, distribution of stages remained nearly constant but proportion of cases radically operated on increased by 3%. Comparison of the regions of Berlin and Erfurt showed only small differences. Compared with international reports, survival rate is very low in Berlin, presumably due to complete registration of new cases and high number of very old people with colonic and rectal cancer.

Adult↗
PubMed1975

[The preparation and characterization of transfer factor (author's transl)].

A transfer factor (TF) was prepared and characterized from the leucocytes of Mantoux-positiv donors using a variety of techniques. The results allowed the following conclusions: Whilst extraction by dialysis and phenol (without precipitation) led to nearly the same positiv immunological activities, when assayed in the MEM-test, phenol extracts subjected to RNA-precipitation lost considerable activity. The residual activity was associated with the lower molecular fractions, and RNA itself has shown no activity. Residues after leucocyte dialysation have also shown considerable TF-activity when extracted by phenol. It was found that phenol extraction followed by gel filtration allowed recovery of considerable quantities of active TF, confined to several specific filtration fractions. Combination of active and inactive gel filtration fractions suggested that there existed an inhibitory factor within the extracted leucocyte preparations. This modified preparation and purification procedure may allow the preparation of TF for therapeutic purposes.

Chromatography, Gel↗
PubMed1975

[A new butyric acid-producing bacteroides species: B. splanchnicus n. sp. (author's transl)].

Three butyric acid-producing saccharolytic Bacteroides cultures (1651/6, BM 158, and IPP 3751) were described by WERNER and REICHERTZ in 1971 (Zbl.Bakt.Hyg., I. Abt. Orig. A 217,206-216). Since then, 6 strains closely resembling 1651/6 were isolated from stool specimens and surgically removed appendices. In the present communication, strains 1651/6, S2/34, S3/38, S4/28, S6/6, A5/2 are described as members of a new species, Bacteroides splanchnicus n.sp. The strains were morphologically very similar (Gram negative non-sporing non-motile rods, 1-2.5 mu in length and 0.7 mu in width) and fermented glucose, fructose, galactose, mannose, lactose, and arabinose (pH values of 4.6-5.4, moderate gas formation). Negative reactions (pH values of 5.8-7.2) were observed with 20 other carbohydrates. The strains were positive in the glutamic acid decarboxylase test and formed indole and H2S. In peptone-yeast extract broth and peptone-yeast extract-glucose broth acetic, propionic, isobutyric, butyric, and isovaleric acids were produced. Washed cells of strains 1651/6 and S4/28 incubated anaerobically in sterile solutions of single amino acids produced butyrate from lysine only. Abundant butyric acid was also produced from glucose. The in vitro activity of 15 antibiotics on 5 strains was studied by broth dilution tests. Uniformly, the strains showed resistance to aminoglycosides and polymyxins (MIC values, 60-500 mug/ml) and susceptibility to tetracyclines, lincomycin, clindamycin, rifampicin, and erythromycin (MIC values, 0.05-0.5 mug/ml). Chloramphenicol, penicillins, and cephalosporins showed bacteriostatic activity at concentrations of 5-40 mug/ml. The serological behaviour of 5 strains was studied in cross-agglutination and gel-diffusion experiments. Cross-reactivity was pronounced in gel-diffusion tests using rabbit antisera and autoclaved extracts and extracts prepared by repeated deep-freezing and thawing of whole cell suspensions as antigens. However, antisera against the B. splanchnicus strains did not react with antigens of B. fragilis 6869, B. thetaiotaomicron AS 126, B. vulgatus AM 45 a, B. distasonis AII 104 and butyric acid-producing strains BM 158 and IPP 3751 in agglutination and gel-diffusion experiments, and there were no cross-reactions between B. splanchnicus antigens and antisera against B. fragilis 6869 and the other aforementioned strains. The new species has been confirmed by members of the ICSB Taxonomic Subcommittee for Gram negative anaerobic rods. Strains 1651/6 and S4/28 have been deposited in The National Collection of Type Cultures, London (NCTC numbers 10825 and 10826). Strain NCTC 10825 (= 1651/6) is the type of the species, B. splanchnicus.

Acetates↗
PubMed1975

[The serological behaviour of the pyogenic anaerobes, Peptococcus asaccharolyticus and P. prevotii (author's transl)].

Using rabbit antisera against whole-cell adjuvant-free antigens, the serological behaviour of ten butyrate-producing Peptococcus strains, five belonging to P. asaccharolyticus and five to P. prevotii, was studied in cross-agglutination and gel-diffusion experiments. Agglutination occurred in all the homologous and nearly one third of the heterologous antigen-antibody systems. Cross-reactions, however, were clearly restricted to members of the same Peptococcus species.

Agglutination Tests↗