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Biomedical subjects

H Wang

Publications and source records attributed to H Wang.

At least 1,693 records · Page 94Linked to original sources
PubMed1997

[Isolation of coltivirus from mosquitoes in Beijing and it's biological characteristics].

Two strains (Beijing 95-70 and 95-75) of coltivirus were isolated from mosquitoes collected in Beijing during summer-autumn in 1994. The viruses caused cytopathogenic effect on C6/36 cells but not on BHK-21 and Vero cells, and were not lethal for new born mice and three week old mice. The isolates were resistant to 5'-IdU and ether, but sensitive to acid pH 3.0. There were 12 segments RNA shown by polyacrylamide gel electrophoresis (PAGE) and PAGE profile of the two isolates were similar (6-6). The PAGE profile was also similar to that of the TRT2 strain isolated in 1991, but there were at least 8 segments slightly different between the new isolates and TRT2 in band migration distance. Tissue culture cross-neutralization test showed that the new isolates were antigenically related to TRT2 strain identified as coltivirus. Growth curve of the two isolates on C6/36 cells showed that virus titer began to rise at the first day after infection and continued to increase up to 7.0 Log TCID50 per 0.1 ml and maintained a high level until the 21st day after infection. It seems that the virus may cause persistent infection on C6/36 cell.

Animals↗
PubMed1997

[Variation in hepatitis C virus NS 5b nucleotide sequence and its relationship with interferon treatment].

In order to investigate variation in hepatitis C virus (HCV) nonstructural 5b (NS5b) nucleotide sequence and the relationship with interferon treatment, cDNA containing in this region were obtained by reverse transcription-polymerase chain reaction from the sera of 4 HCV RNA positive asymptomatic chronic carriers and 9 chronic hepatitis C patients. The cDNA were then directly analysed by polymerase-chain-reaction-directed sequencing. Compared with three published HCV type II/1b sequences (HCV-J, HC-C2 and HCV-BDS), the homology of HCV was lower in the patients with chronic hepatitis C (93.52% +/- 2.09% average), especially in nonresponders to interferon (92.70 +/- 1.90% average), than in asymptomatic HCV carriers (95.65 +/- 2.61%). In 5 patients, HCV RNA was eliminated by interferon treatment. In the remaining 4 interferon-treated HCV survived patients there were 9-48 nucleotide substitutions in HCV mutants out of 380 nucleotides, resulting 6-20 amino acids changes out of 126 amino acids. These results suggested that NS5b region variants play an important role in maintaining persistent infection and the evolution of NS5b is associated with the effect of interferon treatment.

Adult↗
PubMed1997

[Clonning and sequencing of partial gene of hepatitis G virus (HGV) from Nanjing of China].

Partial gene of hepatitis G virus (HGV) was cloned and sequenced from the serum of a patient with post-transfusion hepatitis C in Nanjing of China by reverse transcription-polymerase chain reaction (RT-PCR). The sequence showed 89.09%, 92.12%, 87.27%, 93.94% nucleotide identity over the corresponding region of HGU44402, HGU45966, HGU36380 in America and HGV isolate in Hebei Province of China respectively. Forty patients with post-transfusion hepatitis C and thirty patients with hepatitis non A-E were detected for HGV RNA by RT-PCR, the HGV RNA positive rate were 10.00% and 6.67% respectively.

Base Sequence↗
PubMed1997

[Studies on peripheral blood stem cells mobilization by short course and high-dose G-CSF].

OBJECTIVE: To explore the effect of short-course, high dose granulocyte-colony stimulating factor (G-CSF) on peripheral blood stem cell (PBSC) mobolization. METHODS: G-CSF 5microg/kg was injected subcutaneously twice daily for 3 consecutive days. On the first day and the fourth day the peripheral blood and bone marrow were collected for CFU-GM, BFU-E, CFU-mix assays and CD34/CD38 detection. A single apheresis of over 8000ml blood processing was performed with CS-3000 blood cell separator. The mononuclear cells were prepared for hematopoietic colony assays and CD34/CD38 detection before and after freezing. RESULTS: After adminitration of G-CSF, the white blood cell count increased significantly. The CFU-GM, BFU-E, CFU-mix yields and CD34+ CD38+ cell and CD34+ CD38- cells also increased significantly (P<0.05). The median of mononuclear cells was 1.56 x 10(8)/kg. After one week freezing, the recovery of mononuclear cells, CFU-GM, BFU-E yields and CD34+ CD38+ cells were about 80% (P>0.05) and CFU-mix yields was 60%; The CD34+ CD38- cells were decreased compared with that of pre-freezing (P<0.05). The median of the reinfused mononuclear cells was 1.27 x 10(8)/kg,CFU-GM 6. 7 x 10(4)/kg, BFU-E 1.6 x 10(4)/kg, CFU-mix 0.32 x 10(4)/kg, CD34- CD38+ cells 1.6 x 10(6)/kg and CD34+ CD38- cells 0.23 x 10(6)/kg. CONCLUSION: Short course and high-dose G-CSF can effectively mobilize the peripheral stem cells. The mononuclear cells, hematopoietic colonies and CD34/CD38 cells were satisfied for the peripheral stem cells rescue.

ADP-ribosyl Cyclase 1↗
PubMed1997

[Clinical study on the antithrombotic effects of Holothuria ldeucospilota acidic mucopolysaccharide].

OBJECTIVE: To study the antithrombotic effects of Holothuria Ldeucospilota Acidic Mucopolysaccharide (HLAM). METHODS: A series of variables were observed before and after treatment with HLAM in normal middle-aged subjects and patients recovering from cerebral embolism or suffering from ischemic heart disease, each of which were divided into two groups according to the dosage administered (60 mg/d and 120 mg/d). RESULTS: The fat metabolism was enhanced in addition to the anticoagulant and the blood viscosity reducing effects. No side-effect was found. CONCLUSION: HLAM is a promising drug for antithrombotic therapy.

Adult↗
PubMed1997

[Molecular characterization of genetic defects in hemophilia in Shanghai].

OBJECTIVE: To detect gene mutations in hemophilia A in Shanghai. METHODS: PCR, denaturing gradient gel electrophoresis (DGGE) and DNA sequencing were used. Fifty Chinese cases of hemophilia A without intron 22 inversion, including 24 severe, 9 moderate and 17 mild cases, were screened. Genomic DNA was amplified using GC-clamped primers covering all the exons and flanking intron regions, excluding the middle portion of extron 14 encoding the B domain, but including the thrombin cleavage sites at AA740 and 1689. The amplified GC-clamped PCR fragments were then electrophoresed on DGGE. The abnormal bands were sequenced. RESULTS: Eleven different mutations were identified, including 5 nonsense mutations, 5 missense mutations and one small deletion. Among them, 3 mutations, 466Lys (AAG)-Thr (ACG), 719Tyr (TAC)-Stop (TAG) and 312Ile (ATC)-xxC have not been reported before. CONCLUSION: Apart from intron 22 inversion, most gene mutations in hemophilia A were point mutations resulted from single base substitution. Generally the genetic defects correspond to the clinical manifestations.

Asian People↗
PubMed1997

[Lasting expression of hF IX mediated by myoblasts in mice].

OBJECTIVE: To develop experimentally gene therapy for hemophilia B by using C2C12 cells and C3H mice as models. METHODS: The retroviral vector containing MCK enhancer and hCMV promoter was constructed and was used to transfect PA317 cells. Then the viral particles were used to transfect C2C12 cells and select the G418r clones,which were further confirmed by Southern, Northern, Western blots. And then the C2C12 cells expressing hF IX protein were injected into C3H mice and hF IX expression in vivo was studied. RESULTS: The expression level of hF IX in vivo was positively correlated with the number of injected cells, and the in vitro expression level of these cell clones. The expression of hF IX could be enhanced and prolonged by immunosuppressive agents. CONCLUSION: Allogeneic myoblasts transplantation leads to immunorejection, but repeated injections at a longer intervals could improve the existing rate of transplanted cells. And this might be helpful to gain insight into the myoblast-mediated gene therapy for hemophilia B patients.

Animals↗
PubMed1997

[Determination of steroids in milk powder and birds' feces by high performance liquid chromatography coupled with evaporative light scattering detector].

In this paper, a new, simple and rapid method for the analysis of steroids by reversed-phase high performance liquid chromatography (HPLC) coupled with evaporative light scattering detector (ELSD) is presented. A good resolution of the 12 steroids is obtained within 35 min with a gradient elution using a binary mobile phase composed of methanol and water. The analytical column used was 2.0mm x 150mm, packed with BDS (particle size 5microm) and the mobile phase flow rate was 0.2mL/min. Nebulizer gas flow rate and drift tube temperature, the two critical parameters for determining the reproducibility and signal-to-noise ratio of ELSD were 2.00mL/min and 95 degrees C, respectively. The response of the ELSD was non-linear as a result of basic light-scattering principles, the calibration curve by plotting lnY vs lnX (Y, X stand for peak area and concentration, respectively) shows a good linearity in the range of 5-50mg/L with a correlation coefficient higher than 0.99 for individual steroid and with relative standard deviation less than 6.92%. This method has been applied to the determination of steroids in milk powder and birds' feces, solid phase extraction on reverse phase bonded C18 (5mL/500mg) was used to treat the samples efficiently. The recoveries of the steroids were between 84.0%-102.8%.

Animals↗
PubMed1997

[Study on microwave hydrolysis for the analysis of amino acids in egg by high performance liquid chromatography].

A new hydrolysis method which only needs 7min--microwave hydrolysis used for the analysis of amino acids in egg by Pico-Tag high performance liquid chromatography (HPLC) is reported. The experimental results show that the satisfactory recovery could be obtained by use of 6mol/L HCL and 1% phenol as hydrolysing solution and a microwave oven with power of 300W. These conditions can also be used for the hydrolysis of other samples, such as rice, meat and so on. The average recovery of this method is 95% with a RSD of 2%. The accuracy is better than the conventional one (hydrolysis 24 at 110 degrees C) and Pico-Tag (hydrolysis 24h at 105 degrees C). Furthermore, the microwave hydrolysis decreases the loss of some unstable amino acids, e.g. Ser, Thr, Tyr, etc. In the process the method is convenient, rapid, accurate and reproducible.

Amino Acids↗
PubMed1997

[Design and application of vacuum manifold for solid phase extraction].

Solid phase extraction (SPE) is now a common method for sample pretreatment. SPE vacuum manifold is a key instrument when SPE is used for sample pretreatment. It was developed by National Chromatographic R. & A. Center. Being connected with a vacuum pump, it can deal with 12 samples simultaneously. The manifold has a unique rotatable cover and can be conveniently rotated when switching between any two steps of SPE. An application of this SPE vacuum manifold in measuring bile acids in human serum was given.

Bile Acids and Salts↗
PubMed1997

[The analysis of C4 and C5 olefins in cracking gasoline].

The paper described the analytical method of butenes and pentenes in cracking gasoline with a boiling point range from initial to 210 degrees C. Beside the C3 (propane + protene) and n, i-C4 = (n-butene + i-butene) combine peaks, all C2-C5 components in the sample were separated on the OV-1 column. The proportion of the combined peaks was determined on the Al2O3 column. The quantitative results were calculated on the data determined from the above one-column analytical systems with a relative standard deviation < 8%. Comparing with the ASTM D2427-87, the simple operation, easy application, good repetition and all component results were characteristic of the method.

Alkenes↗
PubMed1997

[Study of the effects of pulse magnetic fields on hemorrheology of human body].

The results show that middle shear specific viscosity and reductive viscosity (P<0.05), low shear specific viscosity and reductive viscosity of whole blood (P<0.01) decreased significantly in experimental group after 9-day of pulse magnetic fields, comparing with control group, high shear specific viscosity, low shear reductive viscosity (P<0.05) and middle and low shear specific viscosity (P<0.01) of whole blood in experimental group were lower after exposed than before exposed to pulse magnetic fields. The results suggest that the pulse magnetic fields may reduce specific and reductive viscosity of whole blood in human body.

Blood Viscosity↗
PubMed1997

[Analysis on current status of drinking water quality in rural areas of China].

An investigation on drinking water quality in rural areas of 180 counties in 26 provinces, municipalities and autonomous regions of China was carried out. The population surveyed was 89.39 million. 69.6% of which was supplied with ground water. Central water supply systems served 47.1% of population. Quality of drinking water was graded according to the "Guidelines for Implementation of the 'Sanitary Standard for Drinking Water' in Rural Areas". The rate of population supplied with unqualified drinking water was 42.7%. The bacteriological indices of drinking water exceeded the standard seriously. Organic pollution occurred extensively. Some regions supplied with water of high concentration of fluoride.

China↗
PubMed1997

[Determination of copper, zinc, calcium, magnesium and iron microelements in whole blood samples from human's ear using microsample-injection FAAS].

This paper established a new and convenient method for the determination of Cu, Zn, Ca, Mg and Fe microelements content in whole blood samples from human's ear by using flame AAS with microsample injection. The results show that recoveries of these microelements are above 96.0% and relative standard deviation of Zn, Ca, Mg and Fe are less than 2.0%, Cu is less than 3.9%. Satisfactory results are obtained with only 10 microL whole blood samples from human's ear.

Adult↗
PubMed1997

[Studies on determinaion of selenium in drink by GFAAS].

Pd(NO3)2-Triton X-100 was used as matrix modifier for determination of selenium in drink. In this way ashing temperature can be increased up to 1200 degrees C and absorbance can increase by 2.92 times. Interferences of Fe, P and other elements were reduced effectively by means of Zeemen effect background correction. Detection limit is 8.0ng/mL and linear range is from 10 to 120ng/ml, and r = 0.9931.

Beverages↗
PubMed1997

[A laplace transform method for analysis of fluorescence dynamics].

A Laplace transform formula is developed for analysis of a multi-exponential fluorescence dynamic process. A dynamic spectrum function is introduced, calculation of which directly yields parameters of the corresponding dynamic process. The formula is applied to analyze fluorescence dynamics of beta subunit from R-phycoerythrin. It is demonstrated that electronic excited-states of the biomolecular system exhibit two exponential decay processes, the faster of which relates to excitation equilibrium among chromophores, and the slower to radiative relaxation of the chromophores.

English Abstract↗
PubMed1997

[Study on the arylaminothiourea compounds by IR spectroscopy].

In this paper, eleven new thiourea compounds were prepared and studied by IR spectroscopy. The fundamental vibrations have been assigned. The band near 1540cm(-1) was identified as thiourea I band, which mainly originated from the N-H deformation vibration coupling with a little C-N stretching vibration. Thiourea I band near 1280cm(-1) mainly originated from C-N stretching vibration. C=S stretching vibration frequency was at near 110cm(-1) and it was identified as thiourea III band.

English Abstract↗