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Biomedical subjects

H Wang

Publications and source records attributed to H Wang.

At least 1,657 records · Page 92Linked to original sources
PubMed1997

[The research on p53 gene mutation in lung cancer tissue of silicotic patients by PCR-SSCR].

Using polymerase chain reaction single strand conformation polymorphism analysis (PCR-SSCP), the authors mutations in exon 5, 7 and 8 of the p53 gene in lung cancer tissue from 15 of 36 silicotic patients. Mutations existed in exon 5, exon 7 and exon 8, but occured more frequently in exon 8. The authors also found that p53 gene mutation rate in lung adenocarcinomas of silicotic patients was higher than that of those without silicosis. A single base substitution was found by DNA sequencing analysis in sample C8. As a result, No. 144 codon was mutated from CAG to AAG, so Gln was substituted by Lys. The authors' data suggest that p53 mutation may play an important role in the pathogenesis of lung cancer of silicotic patients.

Adenocarcinoma↗
PubMed1997

[Experimental study on the influence of sodium glutamate on embryonal brain development of mouse].

The main component of gourment powder is a typical excitatory amino acid. Its neurotoxic action has been attended extensively. Healthy Kunming strain mice were used in this study. Sodium glutamate was injected into peritoneal cavity from the fourth day of gestation (at dosages of 60, 30 or 10 mg/kg every other day). The embryonal mice were dissected at the eighteenth day of gestation. The brain of embryonal mouse was removed, weighted, and fixed in Carnory's solution. Routine paraffin section was made. HE, Feulgen and Nissl staining methods were used for morphological observation. The ash values of 100 neurons of cerebral cortex of Feulgen and Nissl staining were randomly measured with an automated image analyser. The results showed that there was no morphological change in the experimental and control group. There was no significant differece between experimental groups and control group in the parameters observed (P > 0.05). It was suggested that sodium glutamate was not harmful to embryonal brain development of mouse.

Animals↗
PubMed1997

[Experimental and clinical research of cytolytic T lymphocytes specific for hepatocellular carcinoma].

To investigate a new kind of anti-tumor immunological cells and improve surgical results of hepatocellular carcinoma by anti-recurrence application, we activated T cells isolated from tumor infiltrating lymphocytes by double stimulating signals: the one was autologus HCC cells which were treated with IFN-gamma and TNF-alpha for enhancing expression of MHC class I and presented tumor antigen, and the other was costimulation signals which was from ICAM-1 and B7 molecules expressed on treated HCC cells as well as CD28 mAbs. Activated T cells which bound to HCC cells were expanded selectively as tumor specific cytotoxic T lymphocytes (TS-CTLs), their cytotoxic activity in vitro and anti-tumor effects in vivo were observed. Our results suggested that TS-CTLs expressed high cytotoxicity against autologous HCC cells with MHC class I restriction manner. Adoptive TS-CTLs treatment could decrease serum AFP level, inhibit ascites formation and prolong survival in SCID mice bearing human HCC. In clinical trail of 12 cases of HCC, TS-CTLs treatment was able to delay tumor recurrence after HCC resection. Our data demonstrated that TS-CTLs as new immunological treatment modality, are of great value in further application of tumor comprehensive management.

Adult↗
PubMed1997

[The antibacterial activity of cefmetazole and other antibiotics to 463 isolates].

We compared the in-vitro antibacterial activity of cefmetazole and other 4 antibiotics against clinical isolates. The minimal inhibitory concentration (MIC) of cefmetazole against 463 isolates was determined by standard (NCCLS) agar dilution testing, and compared with that of cefazolin, cefuroxime, cefotaxime and ceftazidime. The results showed that cefmetazole was the most effective in 5 antibiotics to indole (+)P. vulgaris and M. morganii (MIC(90) 8 mg/L), and cefmetazole had the same activity as cefotaxime (sensitive 82%-100%), but less activity than ceftazidime (sensitive 88%-100%) against E. coli, K. pneumoniae and P. morabilis. To oxacillin-sensitive staphylococci, cefmetazole was highly active, with the MIC 90 of 4 mg/L. Our results indicate that cefmetazole is highly active against Enterobacteriaceae and oxacillin-sensitive staphylococci.

Cefmetazole↗
PubMed1997

[Expression of Fas and Fas ligand in liver tissue infected with hepatitis B virus].

Paraffin-embedded liver tissue from 60 biopsied or autopsied cases, including 20 cases each of acute mild hepatitis, chronic active hepatitis and active cirrhosis were studied with immunohistochemical double labelling technique by using polyclonal anti-Fas and anti-Fas ligand. The detection rates for Fas and Fas ligand were 76.7% (46/60) and 70.0% (42/60), respectively. Fas antigen was located in cytoplasm of hepatocytes. Fas ligand was expressed mainly in infiltrating lymphocytes in portal or periportal areas (34/42, 80.9%) and also in the cytoplasm of some hepatocytes (25/42, 59.5%). The distribution of Fas ligand-positive hepatocytes was similar to that of Fas-positive hepatocytes in liver tissue. The positive cells were scattered in the intralobular areas in acute mild hepatitis, but they were more commonly aggregated in periportal areas, especially near the edges of the piecemeal necrosis region or in infiltrating mononucleocytes in chronic active hepatitis and active cirrhosis, Double labelling studies showed that both Fas and Fas ligand might be expressed in the same or different hepatocytes of the same area. Our results suggest that Fas-Fas ligand system may play an important role in liver cell injury due to hepatitis B virus infection.

Acute Disease↗
PubMed1997

[The application of corticosteroid following photorefractive keratectomy].

OBJECTIVE: The application methods of corticosteroid following excimer laser photorefractive keratectomy (PRK) were compared. METHODS: PRK was performed on 600 myopic eyes with Nidek-EC 5000 PRK System, and then randomly they were divided into group A (228 eyes) and B (372 eyes). Different methods of application of corticosteroid were performed in the two groups. The follow-up period was 6 months. RESULTS: After 6 months of follow-up, the rate of uncorrected visual acuity > or = 0.8 or > or = 1.0 was 97.9%, 93.2% in group A and 98.6%, 93.5% in group B, respectively. Transient ocular hypertension occurred in 2.2% in group A and 2.7% in group B, but the difference was statistically not significant (P > 0.05), the rate of haze greater than grade 1 was 18.4% in group A and 5.4% in group B, with statistically significant difference (P < 0.05). CONCLUSION: After PRK, the appropriate application of corticosteriod can effectively reduce the rate of haze formation and regression towards myopia caused by haze. Powerful corticosteroid used at postoperative early stage and its low concentration at later stage can reduce the incidence of corticosteroid ocular hypertension.

Administration, Topical↗
PubMed1997

[Histological and ultrastructural studies of experimental herpetic keratitis in the rabbit].

OBJECTIVE: In order to investigate the histological and ultrastructural changes at different post infection days in herpes simplex keratitis (HSK). METHODS: The corneas of New Zealand white rabbit eyes experimentally infected with primary HSK were studied by light microscopy and electron microscopy. The histological and ultrastructural changes in the rabbit corneas at 3, 7, 14, 21, 60 post infection days were investigated by light microscopy with HE, Mallory dyes and electron microscopy. RESULTS: Infiltration of corneas with inflammatory cells was found in rabbit eyes infected with HSK, polymorphonuclear leukocytes being predominant. Contact of inflammatory cells with fibroblast cells and corneal edema were seen in the stroma on acute post infection days 3 and 7. In severe cases inflammatory cells incarcerated in the endothelium. Only did two cases of the specimens present with herpes simplex virus type I granules in the acute stage under electron microscopy. CONCLUSION: The direct HSK-1 destruction together with immunopathogenetic injury caused by the body immune system may serve as the pathologic base of HSK. Decompensation of corneal endothelial function due to infiltration with inflammatory cells may be responsible for corneal edema.

Animals↗
PubMed1997

[Immunohistochemical study on the distribution and coexistence of SP, VIP and NPY in the rat submandibular gland].

The distribution and coexistence of SP, VIP and NPY in the submandibur gland of rat have been studied with immunohistochemical ABC methods. The results indicated that SP, VIP and NPY-IR nerve fibers were presented as varicose and linear profiles, which mainly travelled around acini, duct and blood vessels. The ganglion cells in submandibular gland showed SP, VIP and NPY-IR all positive immunocytochemically. The findings indicated that the neuropeptides were coexisted in the same neuron body and might play the role of regulation of the secretary activity and effective blood supply.

Animals↗
PubMed1997

[Experimental studies on somatic gene therapy for diabetes. I. Structuring of recombinant from human insulin gene and ammalian expression vector PRC/CMV].

To develop a model somatic gene therapy system for diabetes, we constructed a human insulin expression vector in non B cells. As the first step, an insulin cDNA fragment of 260 bp, generated by a complet digestion of PBCA with EcoR I and BamH I, was inserted into the EcoR I/BamH I site of plasmid PBS.SK by ligation of cohevise-ended DNA to construct transition plasmid PBS.INS. Then the plasmid PBS.INS was completly digested by Hind III and Xbal I. The small DNA fragment containing insulin cDNA gene was subcloned to the expression plasmid PRC/CMV to form recombinant PRC/CMV.INS.

DNA, Complementary↗
PubMed1997

[Detection of antineutrophil cytoplasmic antibodies for the values of Wegener's granulomatosis diagnosis and activity judgment].

OBJECTIVE: To investigate the clinical significance of antineutrophil cytoplasmic antibodies (ANCA) in Wegener's granulomatosis (WG) and other diseases. METHOD: Indirect immunofluorescence was applied to detect serum ANCA of 204 patients and 48 normal controls. Enzyme-linked immunosorbent assay (ELISA) was used to detect antimyeloperoxidase (MPO) antibodies. RESULTS: The frequency of ANCA in sera of WG patients was 75% and significantly higher than those in sera of other groups (P < 0.05). ELISA demonstrated that MPO was the major antigen recognized by perinuclear ANCA (P-ANCA). Cytoplasmic ANCA (C-ANCA) was always positive and high titers in sera of active WG patients, but turned to negative or low titers in remission. CONCLUSION: These results suggested that C-ANCA was specific for WG and was a marker for disease activity in WG. P-ANCA wasn't specific for WG, but related to vasculitis.

Antibodies, Antineutrophil Cytoplasmic↗
PubMed1997

[The usefulness of dye endoscopy in diagnosis esophageal diseases].

OBJECTIVE: To investigate the usefulness of Dye endoscopy in the diagnosis of the esophageal disorders. METHODS: 87 cases with esophageal diseases underwent dye endoscopy examination with Toluidin-staining and Iodine-staining or double-staining method. RESULTS: In the conventional group, the conformity rate of the endoscopy to biopsy results is 80%, but in the Dye one is 95% and the accurate rate of diagnostic is 100%. Under the guiding of Dye endoscopy, the occurrence rate of Ca cell on the incisal edge is 0%. CONCLUSION: The methods enable diffentinal diagnose of benign and malignant lesion, conforming the type of esophagitis. And it is more helpful to conform the area of surgical resection and when conventional endoscopy cannot satisfactorily diagnosis. We think it is a useful and simple diagnostic method for esophageal diseases.

Diagnosis, Differential↗
PubMed1997

[A preliminary report of histological study on TMJ reconstruction with autogenous costochondral graft].

To study histologically the biological behaviour of the grafts and the reactions in the receipt area after reconstruction of temporomandibular joint (TMJ), we have reconstructed experimentally removed TMJs on purebred juvenile pigs by transplanting autogenous costo-chondral grafts. The findings of continuously survey of histologic changes in the receipt areas and the grafts showed that autogenous grafts healed well with the surrounding soft tissues, and induced the mesenchymal-like cells from the receipt region, rather than the periosteal cells which might be left, to differentiate and ossify both within and at the surface of them, and they themselves were gradually replaced by the new-formed bone; and that active osteogenesis could also be seen within the thick tissues far from the graft, which could not be the result of graft's induction. It may be concluded that the new bone, both from the induction of the graft which acts as a framework and somehow from the outside tissues, reconstructs the new condyle and determines the postsurgical growth of the mandible.

Animals↗
PubMed1997

[Correlations of T lymphocyte subsets and IL-2, sIL-2R in patients with malignant ovarian tumor].

To study the relation between malignant ovarian tumor (MOT) and cell mediated immunity, the numbers of T lymphocyte subsets and levels of IL-2 and sIL-2R were measured simultaneously in 40 patients with MOT and 30 normal controls (NC), and the correlations between T subsets and IL-2, T subsets and sIL-2R were analyzed. The results were as follows: (1) The numbers of CD3+, CD4+, CD4+/CD8+ and the levels of IL-2 in the patients were lower than those in the NC significantly, and the opposite results were observed about CD8+ and sIL-2R (P < 0.01-0.001); (2) There were positive correlations between CD3+, CD4+, CD4+/CD8+ and IL-2 and there were negative correlations between CD3+, CD4+, CD4+/CD8+ and sIL-2R in MOT group (P < 0.01-0.001). The results suggested that the T lymphocyte immune function in MOT group was decreased. The reduced numbers of CD3+, CD4+, CD4+/CD8+ were related to the decreased levels of IL-2 and the increased levels of sIL-2R. It may be an important factor of incidence and development in MOT.

Adult↗
PubMed1997

[The compressive strength, tensile strength, flexural strength and micro-hardness of Plat-II Castable Ceramics].

In preparation for the clinical use of Plat-II Castable Ceramics (PCC-II), we tested its compressive strength, tensile strength, flexural strength and microhardness. The flexural strength was tested by the three-point bending test. The result showed that the compressive strength was 541.7 MPa; the tensile strength 42.5 MPa; the flexural strength 142.0 MPa; the modulus of elasticity 61.4 GPa; and the micro-hardness 499.6 kg/mm3. The strength anol micro-hardness of PCC-II materials were better than those of human enamel. So PCC-II is applicable to restorations in dentistry.

Ceramics↗
PubMed1997

[The influence of porcelain thickness and non-uniformity on porcelain cracks in implant-supported metal-porcelain fixed bridge].

This experiment studied the influence of porcelain thickness and non-uniformity on porcelain crack in implant-supported metal-porcelain fixed bridge. The result indicated that porcelain crack began to appear when the body porcelain powder was 3-5 mm in thickness; more cracks took place when the powder thickness increased by 1 mm to 2 mm on the axial surface in the axial direction; cracks became serious when the thickness suddenly changed to zero; and connectors were liable to cracks. Therefore, in designing and fabrication, one should avoid and sudden change in the shapes of the connector and the porcelain on it, any sudden change in the thickness of porcelain and a thickness of porcelain powder not less than 3.5 mm. Other-wise, it is neccessary to use internal crown between metal base and abutment to meet the demands.

Dental Porcelain↗
PubMed1997

[P53 protein expression in malignant germ cell tumor of ovary and its relationship with clinical course and prognosis].

To study the relationship of P53 protein expression with clinical course and prognosis in malignant germ cell tumor of ovary P53 protein expression was examined by immunohistochemical ABC methods in 82 cases of the neoplasm and 20 cases of normal ovary tissue. The results showed that no expression of P53 protein was detected in normal ovarian tissue. The total expression rate of P53 protein was 24.39% in neoplasm, and the expression was not significantly correlated with the different histological types of neoplasm. The study however found that the protein expression of p53 gene was significantly correlated with the clinical stages and prognosis of the neoplasm.

Choriocarcinoma↗
PubMed1997

[Application of spectrophotometry to evaluation of the levels and the influence of chemotherapeutic drugs on ATP of malignant cell lines].

To evaluate the value of ATP spectrophotometry for malignant tumor in vitro chemosensitivity test, Hela and L929 cell lines, which were derived from human cervical carcinoma and mouse fibroma respectively, were investigated. The results showed that when the cellular numbers were > or = 2 x 10(5), the linearity between the ATP concentrations and the cellular numbers was observed, with all 2 cell types studied, and that when L929 cells were incubated with cis-platinum (1x, 2x, 5x x plasma peak concentration, PPC), a marked fall in ATP concentrations was observed and a clear dose-response curve was obtained, but no decrease of ATP concentrations was measured after incubation with adriamycin (1x, 2x, 5x x PPC). This study suggests that ATP spectrophotometry is a cheap, feasible method for studying the energy metabolism and the influence of chemotherapeutic drugs on ATP concentrations of malignant tumor.

Adenosine Triphosphate↗