Search PubMed⌕ Search

Biomedical subjects

H Wang

Publications and source records attributed to H Wang.

At least 1,261 records · Page 70Linked to original sources
PubMed1999

[Clinical study of capsaicin in the treatment of allergic rhinitis].

OBJECTIVE: To study the clinical effect of capsaicin of nasal administration in 100 cases of allergic rhinitis. METHOD: Capsaicin was applied by nostril, once 5 days and 5 times as a therapeutic course. Acidophil were measured and nasal airflow resistance was analysed before and after capsaicin therapy. RESULT: It showed that nasal symptoms of patients were obviously relieved and 94% of the cases in the measure of acidophil changed to negative from positive. Moreover, capsaicin induced decrease in nasal airflow resistance from (0.6272 +/- 0.3534) kPa.s/L before therapy to (0.4057 +/- 0.2419) kPa.s/L after therapy (P < 0.001). 90 cases of 100 patients were fellowed up one year, the total effective rate was 89%. CONCLUSION: Our study suggests that capsaicin possessed remarkable effects in the treatment of allergic rhinitis; analyse of nasal airflow resistance may be used as one of the objective guideline in the determinant of therapeutic effects.

Administration, Intranasal↗
PubMed1999

[Characterization of expression of the apoptosis associated PF18-3 molecule in thymocyte apoptosis following ConA activation].

The expression pattern of McAb PF18-3 recognized molecule in activation induced thymocyte apoptosis was analysed. Results indicated that following activation by ConA, thymocytes underwent activation induced apoptosis identified by delayed production of DNA ladder and TUNEL positive staining of thymocytes in late stage of activation. PF18-3 molecule was found to express specifically in subset of apoptotic thymocytes with diploid. Kinetic comparison of expression between PF18-3 molecule and Fas or translocated membrane phospholipid suggested that PF18-3 molecule is different from them and likely to be a novel molecule related to thymocyte apoptosis.

Animals↗
PubMed1999

[Determination of alpha-methoxynaphthalene by reversed-phase high performance liquid chromatographic method].

A reversed-phase high performance liquid chromatographic method was developed for the quantitative determination of alpha-methoxynaphthalene and it's harmful impurity. The instrument used was Varian Model 5000 HPLC system, with Nucleosil-C18 column, 10 microns, 300 mm x 4.6 mm i.d., eluent of V (methanol):V(water) = 90:10 at a flow rate of 0.8 mL/min and detection wavelength, 254 nm. The relative purity of alpha-methoxynaphthalene was more than 96% and relative standard deviation was 0.29%.

Chromatography, High Pressure Liquid↗
PubMed1999

[Screening and analysis of biologically active components in traditional Chinese medicine by molecular biochromatography].

Screening of biologically active components from Chinese medicines by molecular biochromatography has been first proposed by authors. This paper summarizes their recent progresses on screening and analysis of Chinese medicines by molecular chromatography, including the comparison of the chromatograpic pattern for a number of herbal medicines, and the same kind of medicines but different sources, method development for screening and quality control of Chinese medicines, and the study on the interactions between the biologically active compounds in Chinese medicines and protein. The prospect of molecular biochromatography on study of Chinese medicines has also been highlighted.

4-Butyrolactone↗
PubMed1999

[A study of time-dependent blood flow distribution in ischemic myocardium and its dependence on perfusion pressure with modeling and emulation].

A mathematical model was proposed for studying the time-dependent blood flow distribution in ischemic myocardium with different developments of collateral circulation. The model includes the blood perfusion of normal myocardium and ischemic myocardium. There are three classical myocardial vascular nets in each part and each net is represented by a branch of time-dependent nonlinear resistance and compliance. Experimentally measured aortic pressure and left ventricular pressure from six male dogs were taken as input data for emulation. The results demonstrate that raising perfusion pressure with external counterpulsation can improve the blood perfusion condition in ischemic myocardium.

Animals↗
PubMed1999

[Study on the separation of optical chromatography].

The relationship between the particle radius with the intensity of the applied laser radiation force is theoretically studied using a ray-optics model by analyzing the forced-status of the separated particles in the mobile phase. The laser radiation force is proportional to the square of the particle radius when the particle radius is much smaller than the laser beam radius under the condition of using a single-transverse-mode laser beam with a Gaussian intensity distribution and the particle refractive index being larger than that of the mobile phase. The retention distance is calculated and the dynamic range is discussed. Theoretically, the dynamic range of the optical chromatography can be extended by increasing laser power, decreasing the flow rate of mobile phase and focusing the laser beam.

Chromatography↗
PubMed1999

[A novel chiral selector in capillary electrophoresis--beta-cyclodextrin polymer].

Three chiral selectors CM-beta-CD, EP-beta-CD, beta-CD were studied for the enatio-separation of three drugs under optimum conditions respectively. The results demonstrate that the resolving power for the drugs is as follows CM-beta-CD > EP-beta-CD > beta-CD, with the exception of lobeline. This is due to the--CH2COOCH3 group of CM-beta-CD, which will change the combination and improve the recognition on guest molecules. Although EP-beta-CD is inferior to CM-beta-CD for the separation of chlorpheniramine and verapamil, it has excellent recognition on lobeline and it has not been reported previously. In most cases EP-beta-CD is superior to beta-CD. The explanations are: (1) EP-beta-CD has good solubility in water, which enables high concentrations to be used and consequently achieves excellant separation of racemic compounds, (2) the polymerization of beta-CD changes the properties of CD units and the process produces a more rigid and different conformation from CD, (3) we must attribute much merits to the cooperation or synergism of two, three or even more CD moieties of two polymers for inclusion. Complexation with analytes possesses more than one guest part in their structure.

Anti-Allergic Agents↗
PubMed1999

[Recognition and classification of heart sounds].

This paper reviews the methods and research advances of recognition and classification of heart sounds. The methods include the statistical techniques and artificial neural network. It also discusses the main problems in the methods.

Algorithms↗
PubMed1999

[The advances of the study on the optical chromatography].

This review is mainly concerned with the recent developments in (1) the differences between the optical tweezer and optical chromatography; (2) the principle of optical chromatography separation; (3) the theory studies on the optical chromatography, especially the relationship between the particle radius with the intensity of the applied laser radiation force and the relationship between the particle retention distance with the particle radius; (4) the application of the optical chromatography technique to the separation of polymers, biological macromolecules and biological cells. The application of optical chromatography to the immunoassy and molecular biology is highlighted.

Chromatography↗
PubMed1999

[The present research situation of cause of formation of impedance cardiograph waveform].

This paper summarized the present research situation of cause of formation of impedance cardiograph waveform in two aspects of early and current research on it by the means of consulting a great deal of domestic and foreign data. The paper pointed out that a practicable way for correcting Kubicek theory and its physical model, making SV formula more accurate is to apply finite element technology to the research field under the guide of impedance cardiograph electric field theory. At the same time, the paper made preliminary introduction on the author's research work in the area.

Animals↗
PubMed1999

[Influence of EBV latent membrane protein 1 gene on the proliferation of epithelial cells].

In order to study the influence of EBV latent membrane protein (LMP1) gene on the proliferation of epithelial cells and the role of LMP1 creating to epithelial cell carcinomas, EBV LMP1 gene eukaryotic expression plasmid was transfected into human embryo kidney epithelial cells. The expression of LMP1 protein in transfected cells was detected. Observing the formation of colonies in soft agar, the value of MTT absorbed and the expression of PCNA, the results showed that the transfected cells grew vigorously and formed more colonies in soft agar, the MTT uptake and PCNA expression were apparently increased. The conclusion was that LMP1 gene could alter the biological behaviours of epithelial cells, promote cell growth, proliferation and transformation and endowed the transfected cells with growth characteristics like tumor cells.

Cell Division↗
PubMed1999

[Fraction P isolated from the ribosomes of Serratia odorifera and its tumor inhibiting effect].

A protein(component P) with molecular weight of 16 kD was isolated by the method of preparation electrophoresis from the ribosomes of Serratia odorifera, which were obtained by the method of differential centrifugation. This protein showed specific immune response reactivity with the antiserum of the ribosomes mentioned above. Meanwhile, component P showed apparent protective function for QGY-tumorcharged nude mouse and different ways of giving medicine. It was evident from study results that component P isolated from the ribosomes of Serratia odorifera showed a direct function for tumor growth inhibition and it was the effective component of the ribosomes of Serratia odorifera.

Animals↗
PubMed1999

[First discovery of Taenia saginata asiatica infection in Yunnan province].

AIM: To investigate the aetiology, species and epidemiological factors of Taenia infection in a pilot area of Lanping County, Yunnan Province. METHODS: Two patients with taeniasis were treated with antiscolex capsule and praziquantel, respectively. Five local weaning pigs free from tapeworm infection were fed with gravid proglottids obtained from the patients. After 2-3 months, the pigs were necropsied to examine cysticerci. In addition, one pig naturally infected with Taenia was treated as well. RESULTS: Based on morphological characteristics of the scolex and gravid proglottid of four tapeworms obtained from the patients, the worms were similar to T. saginata. 23 cysticerci recovered from two experimentally infected pigs were found in the livers, omentum majus and mesenterium, and 3 cysticerci were found in the liver and omentum majus in a naturally infected pig as well. The protoscolex of mature Cysticercus had two rows of rudimentary hooklets, one rostellum and four acetabula resembling to C. cellulosae. According to morphological characteristics and the location of cysticerci, the tapeworm was identified as Taenia saginata asiatia. CONCLUSION: T. saginata asiatica infection was first reported in Yunnan Province.

Adult↗
PubMed1999

[Clinical value of distortion product otoacoustic emission (DPOAE) and their contralateral suppression effects].

OBJECTIVE: The present paper aimed at examining the role of DPOAE and their contralateral suppressive effects in evaluation medial olivocochlear system (MOCS) function status in human. METHOD: Seventeen subjects (34 ears) with normal hearing, 13 ears with cochlear hearing loss and 9 ears with retrocochlear hearing loss were studied on their DPOAE and contralateral suppression. RESULT: The results revealed that DPOAE amplitude significantly reduced in ears with cochlear hearing loss, contralateral suppression was smaller than normal ears but there was no significant difference between two groups. By contrast, DPOAE amplitude was much higher in ears with retrocochlear hearing loss than that in the other two groups. Contralateral suppression was significantly reduced or disappeared, and contralateral acoustic stimulus (CAS) even increased DPOAE amplitude in ears with retrocochlear hearing loss. CONCLUSION: DPOAE and suppression induced by CAS can be used as an index of medial olivocochlear system (MOCS) function in patients with sensori-neuaral hearing loss, and can differentiate cochlear hearing loss from retrocochlear hearing loss. The tests of DPOAE and contralateral suppression may have much more advantages in clinical application over any other audiological test.

Acoustic Stimulation↗
PubMed1999

[Clinical significance of detecting telomerase activity in ascites cells from patients with ovarian carcinoma].

OBJECTIVE: The purpose was to investigate the clinical significance of telomerase activity for tumor diagnosis in ascites cells from patients with ovarian carcinoma. METHODS: Thirty ascites specimens from patients with ovarian tumor were examined for telomerase activity by PCR-based TRAP (telomeric repeat amplification protocol) method, and the result of detection was compared with the cytological diagnosis. RESULTS: Telomerase activity was detected in ascites cells from patients with ovarian tumor in 19 of the 20 cytologic-possitive specimens and in 3 of the 7 cytologic-negative specimens and in all the 3 cytologic-suspicious specimens. The positive rate in 30 ascites samples by cytological examination and by the detection of telomerase activity was 66.67% (20/30), and 83.33% (25/30), respectively. CONCLUSIONS: The result suggested that measuring telomerase activity in ascites cells would be helpful to cytological examination in diagnosis of patients with ovarian carcinoma.

Ascites↗