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Biomedical subjects

H Wang

Publications and source records attributed to H Wang.

At least 1,225 records · Page 68Linked to original sources
PubMed1999

[The expression and regulation of heme oxygenase-1 in asthmatic guinea pigs].

OBJECTIVE: To explore the expression and pathogenic mechanism of heme oxygenase-1(HO-1) in asthma. METHODS: Two groups of guinea pigs (10 in each) were treated with the specific stimulator(hemin) and inhibitor(Sn-PP) of HO-1 respectively. Their effects on the levels of HO-1 activity, COHb, cGMP and IgE in serum or blood and lung tissues were compared with each other. Acute asthmatic group (AAG), dexamethasone-prevented group(DPG), stable asthmatic group, ovalbumin-sensitized group and normal control group(NCG) were also included. Lung tissues of tested animals were pathologically observed and immunohistochemically stained. RESULTS: In the AAG and hemin stimulated group, the levels of COHb, cGMP and IgE were significantly increased with higher levels of activity and expression of HO-1 protein compared with the NCG (t = 4.575-10.188, P < 0.01). In DPG and Sn-PP inhibited groups, however, all measured parameters were markedly reduced compared with the AAG(P < 0.01). The other groups were normal. CONCLUSIONS: The increased production and activity of HO-1 resulted in increases of endogenous CO and cGMP and promoted inflammation and immunoreaction in asthma.

Animals↗
PubMed1999

[The effect of erythromycin on NF-kappa B activation and cytokines mRNA expression in bleomycin-induced pulmonary fibrosis in rats].

OBJECTIVE: To evaluate the effect of erythromycin (EM) on nuclear factor-kappa B (NF-kappa B) activation and cytokines mRNA expression in bleomycin-induced pulmonary fibrosis in rats. METHODS: Wistar rats were divided into three groups of 27 as follow: BLM-group received intratracheal instillation of single dose BLM 5 mg/kg. EM treated-group received intratracheal instillation of BLM 5 mg/kg and an oral instillation EM 100 mg.kg-1.d-1. Control group received intratracheal and oral instillation of normal saline. Animals of all three groups were sacrificed on the 4th, 7th and the 28th day separately. NF-kappa B activation in alveolar macrophages (AM) was investigated by electrophoretic mobility shift assay. The expressions of IL-1 beta and TGF-beta mRNA in lung were evaluated by Northern blot analysis. The pathological changes of lung tissue were analyzed quantitatively by computer gray scan. RESULTS: On the 4th and 7th day, the activities of NF-kappa B in AM were significantly increased in BLM-group compared with those of control group (P < 0.05), while they were significantly decreased in EM treated-group compared with BLM-group (P < 0.05). On the 7th day, the expressions of IL-1 beta and TGF-beta mRNA in lung were significantly decreased in EM treated-group compared with those in BLM-group. Pathologically, EM decreased exudation of inflammatory cells in the early response as well as degree of fibrosis in the late stage in the BLM-induced pulmonary fibrosis. CONCLUSIONS: These results indicate that NF-kappa B is involved in the inflammatory response in BLM-induced pulmonary fibrosis in rats, EM has inhibitory effect on NF-kappa B activation, IL-1 beta and TGF-beta mRNA expression, by which it ameliorated acute lung injury and fibrosis those tested rats.

Animals↗
PubMed1999

[Correlations between invasion of gastric cancer to serosal layer and other relevant factors].

OBJECTIVE: To study correlations between invasion of stomach cancer to serosal layer and such factors as size and type of tumor, depth of ulcer, gastric wall contraction, etc. METHODS: Gastric cancer specimens from 150 patients admitted to the hospital in March 1993 through March 1997 were examined. RESULTS: The frequency of tumor invasion to the serosa in gastric cancer of Borrmann type I, II, III and IV was 8.3%, 23.4%, 32.9% and 76.2%, respectively. The frequency of serosal involvement was 13.6% in tumors with a diameter < or = 3.0 cm which was significantly less frequent than in tumors with a diameter > 3.0 cm. The frequency of serosal involvement increased with the increase in depth of ulcer and with spastic contraction of gastric wall as observed in pre-operative barium meal examination. That of serosal involvement varied with degree of differentiation of the tumor, being much lower in well- and moderately well-differentiated adenocarcinomas (10.3%) than in poorly differentiated ones (62.8%). The serosa was frequently invaded by mucinous adenocarcinoma (42.9) and signet cell carcinoma (83.3%). CONCLUSION: Invasion of gastric cancer to serosal layer correlates, to various extents, with the tumor size and type, status of cell differentiation, and depth of ulcer.

Adenocarcinoma↗
PubMed1999

[Effect of caoguo zhimu decoction on N-methyl-D-aspartate receptor gene expression of epileptic kindling model in rats].

OBJECTIVE: To explore the effect of Caoguo Zhimu decoction (CGZMD) on N-methyl-D-aspartate (NMDA) receptor gene expression of epileptic model on rat brain. METHODS: Adopting the pentylenetetrazol (PTZ) kindling model to observe the NMDAR1 mRNA gene expression by situs hybridization. RESULTS: After 28 days of CGZMD treatment, the NMDAR1 mRNA gene expression on CA1, CA2, CA3 part of rat hippocampus reduced obviously, the comparison between experimental group and the model control group showed significant difference (P < 0.05). CONCLUSION: CGZMD decreased NMDAR1 mRNA gene expression, it may be caused the NMDA receptor decline, hence revealed its anti-epileptic effect.

Animals↗
PubMed1999

[The effects of blocking intrarenal renin-angiotensin system on the expression of transforming growth factor-beta1 mRNA and extracellular matrix components].

OBJECTIVE: To explore the possible mechanism of benazepril in diminishing the progression of the renal diseases. METHODS: Experimental glomerulosclerosis in rats was induced by adriamycin. The treated group was given benazepril (4 mg x kg(-1) x d(-1)). Intrarenal angiotensin converting enzyme (ACE) activity and angiotensin II (AngII) concentration were measured with colorimetry and radioimmunoassay respectively. Immunohistochemistry staining was employed for transforming growth factor beta1 (TGF beta1) and extracellular matrix (ECM) examination. To observe the expression of TGFbeta1 mRNA in the kidney, in situ hybridization was performed. RESULTS: In the rats treated with benazeapril, ACE activity and Ang II concentration in renal tissue were significantly inhibited. In the meantime, expression of TGFbeta1 mRNA and TGFbeta1 protein were down-regulated, accompanied by decreasing accumulation of ECM (compared with the untreated rats, P < 0.01). CONCLUSION: Benazepril plays an important role in down-regulating the expression of TGFbeta1 and decreasing the accumulation of ECM by blocking intrarenal renin-angiotensin system.

Angiotensin II↗
PubMed1999

[The mediation effects of endothelin-1 on human mesangial cell proliferation stimulated by thrombin].

OBJECTIVE: To investigate the effects of endothelin-1 (ET-1) on mesangial cell proliferation stimulated by thrombin. METHODS: With cultured human mesangial cells (HMC), the following tests were performed. (1) Stimulative tests: After thrombin stimulation, HMC proliferation was determined by MTT method, concentration of ET-1 in supernatant was measured with radioimmunoassay (RIA) and preproendothelin-1 (ppET-1) mRNA expression of HMC was analysed by Northern blot hybridization. (2) Inhibitory test: The influence of ET-1 inhibitors on the HMC proliferation following thrombin stimulation at three different levels, i.e. ET-1 antiserum, selective ET receptor-type A (ET(A)) antagonist and antisense oligodeoxynucleotide for ppET-1, was measured by MTT test respectively. RESULTS: (1) HMC proliferation was stimulated by thrombin in dose dependent manner and in relation to the time of stimulation. The peak growth level was attained in thrombin concentration of 16 U/ml and incubation time of 16 hours. ET-1 secretion by HMC was increased in parallel with the dose curve and time curve of HMC proliferation; HMC ppET-1 mRNA expression was up-regulated by thrombin 16 U/ml with incubation time of 8 hours and 16 hours respectively (7.9 fold, 3.4 fold versus control group). (2) ET-1 inhibitors, ET-1 antiserum, selective ET(A) receptor antagonist and antisense oligodeoxynucleotide for ppET-1 all significantly inhibit HMC proliferation stimulated by thrombin. CONCLUSION: Thrombin stimulates HMC to produce ET-1 and ET-1 mediates the HMC proliferation.

Cell Proliferation↗
PubMed1999

[Misdiagnosis of acute renal failure and the clinical significance of renal biopsy].

OBJECTIVE: To improve the diagnostic accuracy of acute renal failure (ARF) by analyzing the causes of misdiagnosis. METHODS: To compare the correlation between admission and final diagnoses, find the possible causes of misdiagnosis and summarize the influence of early diagnosis and treatment on prognosis. RESULTS: In 111 ARF patients, only 67.6% (75/111) was diagnosed as ARF before admission; 14.4% (16/111) was misdiagnosed as chronic renal failure (CRF); 18.0% (20/111) as renal tumor, urinary lithiasis and hematuria or proteinuria of unknown origin. Renal biopsy was performed in 69 cases. Diagnosis was corrected in 21.7% (15/69) of the cases and the method of treatment was changed in 56.5% (39/69) cases after biopsy. CONCLUSION: It is showed that some cases of ARF were misdiagnosed as CRF. The size of the kidney and finger nail creatinine level would be helpful in the differentiation of CRF and ARF. The difficulty in establishing the causes of ARF is the differentiation between acute glomerulonephritis and rapidly progressive glomerulonephritis (RPGN), acute tubular necrosis (ATN) and acute interstitial nephritis (AIN). ATN or AIN with chronic glomerulonephritis may be confused with RPGN. When there is difficulty in differentiation, renal biopsy should be performed as soon as possible.

Acute Kidney Injury↗
PubMed1999

[Malignant pheochromocytoma: diagnosis and treatment].

OBJECTIVE: To review the experience with the diagnosis and treatment of malignant pheochromocytoma. METHODS: Between 1986 and 1996, 7 patients with malignant pheochromocytoma were analysed. RESULTS: Compared with benign pheochromocytoma of adrenal gland, the malignant one usually exceeded 7.0 cm in diameter, irregular in shape, invading the surrounding tissue and the normal structure of the effected adrenal gland disappeared. Bleeding and necrotic area were seen in tumor mass, even seemed as a cystic lesion with thick wall. In some patients, blood catecholamine and urine VMA significantly elevated without hypertension. The separating phenomenon and progressive weight loss, accelerated ESR were characteristics of malignant pheochromocytoma. CONCLUSIONS: In some patients with malignant pheochromocytoma, histological findings are not consistent with their biological behaviors. Follow-up studies is mandatory in malignant pheochromocytoma as well as in benign one. Recurrence and/or metastasis after operation often occur in patients with malignant pheochromocytoma. In addition to early diagnosis and surgery, radiotherapy of tumor bed and systematic chemotherapy should be emphasized.

Adrenal Gland Neoplasms↗
PubMed1999

[Expression and significance of MXR7 mRNA in human hepatocellular carcinoma].

OBJECTIVE: To find out the expression and significance of MXR7 mRNA in human hepatocellular carcinoma. METHODS: The (32)P labeled and purified PCR product of MXR7 cDNA was taken as probe. MXR7 mRNA expression was detected by Northern blot analysis in 30 samples of preoperatively untreated HCC and corresponding surrounding non-cancerous hepatic tissue, and 12 normal liver tissues confirmed pathologically. RESULTS: The frequency of MXR7 mRNA expression in HCC, the corresponding surrounding noncancerous hepatic, and normal liver tissues was 76.6%, 13.3% and 0, respectively. The frequency of MXR7 mRNA expression in HCC without serum AFP elevation and in HCC < 5 cm was 9/10 and 5/6 respectively. CONCLUSIONS: MXR7 mRNA over-expression in human HCC is common and MXR7 mRNA may be expressed at early stage of HCC and can serve as tumor biomarker for HCC. The detection of MXR7 mRNA expression in liver puncture tissues may find small HCC without serum AFP elevation in the subclinical stage.

Adult↗
PubMed1999

[The action of proglumide blocking gastrin on gastric cancer cells].

OBJECTIVE: To investigate the practicability of proglumide to treat gastric cancer. METHODS: MKN45 gastric cancer cell line was cultured and the effects on gastrin and gastrin receptor antagonist proglumide proliferative rate, cell dynamic cycle distribution and the concentration of cAMP in the cells were observed in vitro. RESULTS: Gastrin promoted the proliferation of MKN45 cells and shifted cells from phase G(0)/G(1) to phase S, G(2)/M as well as increased intracellular cAMP, while proglumide blocked these effects. CONCLUSIONS: Gastrin induces the proliferation and synthesis of DNA by its receptor. Proglumide may provide a new approach of non-cytotoxic treatment of gastric cancer.

Cell Cycle↗
PubMed1999

[The treatment of 432 patients with pituitary adenomas via transsphenoidal approach].

OBJECTIVE: To study some clinical features of 432 cases of pituitary adenomas treated by transsphenoidal microsurgery between July 1982 and October 1996. METHODS: Pathological examination, immunohistochemical examination and electronmicroscopic examination were done in 424, 29 and 103 cases respectively. RESULTS: Hyperprolactinemia was found in many so-called non-functioning adenomas besides in prolactinomas. The dura maters were invaded by tumors in 78 cases (the incidence of dural invasion was 65.5%). CONCLUSIONS: Hyperprolactinemia is not peculiar to prolactinomas, and differential diagnosis is necessary when hyperprolactinemia exists. The involvement of the dura mater of sellar floor is an important mark that the adjacent tissues were invaded by pituitary adenomas. The outcome of surgical treatment is significantly different between the patients with dural invasion and without invasion.

Adenoma↗
PubMed1999

[Synovial chondromatosis: clinical analysis of 13 cases].

OBJECTIVE: To study the diagnosis and treatment of synovial chondromatosis. METHOD: 13 cases of synovial chondromatosis were studied by pathological observation, X-ray examination and clinical research. RESULTS: Intermittent joint pain, swelling, limited motion, joint snapping or rubbing were found, sometimes with joint interlocking and movable mass in the joint. X-ray showed fewer loose bodies than the actual ones in the joint. Rough surface, congestion and thickening of synovial membrane at the lesion were found during the operation. Proliferation of blood vessels, accumulation of lymphocytes and proliferation of synovial superficial cells and fibrous tissue were observed under the microscope. The loose body consisted of many tubercles microscopically. CONCLUSIONS: The combination of clinical observation, X-ray examination and pathological diagnosis is essential to the diagnosis of synovial chondromatosis. Excision of loose body and synovial membrane may lead to satisfying results.

Adolescent↗
PubMed1999

[Prevention of postoperative recurrence of bladder cancer: a clinical study].

OBJECTIVE: To lower postoperative recurrence rate of bladder cancer, the prophylactic effects of five kinds of method on bladder cancer were evaluated. METHODS: Between 1982 and 1997, 313 patients after TURBT or partial cystectomy were followed up for 2 to 15 years (average 7.6 years). These patients were divided into six groups: BCG, mitomycin C (MMC), thiotepa, Chinese herb medicine Zhuling (Grifola umbellata pilat), afterloading brachytherapy and control group. The prophylactic effects of them on postoperative recurrence of bladder cancer was evaluated. RESULTS: During the follow-up, the recurrence rates were 35.1% in BCG group, 34.9% in Zhuling group, 41.7% in MMC group, 52.6% in thiotepa group, 64.7% in control group, respectively. 25 high-risk patients with invading or multiple bladder cancer were treated by afterloading brachytherapy. They were followed up from 12 to 42 months, with a recurrence rate being 24.0%. CONCLUSIONS: The prophylactic effect of Zhuling and BCG on bladder cancer recurrence was better than MMC. The vale of thiotepa was not significant. The afterloading brachytherapy was of great vale to invading or recurrent, multiple bladder cancer.

Adult↗
PubMed1999

[The effect of sandostatin and 5-fluorouracil on apoptosis in breast cancer].

OBJECTIVE: To investigate the effect of 5-FU plus sandostatin on apoptosis in breast cancer. METHODS: Breast cancer samples from 16 patients were treated with 5-FU (10 microg/ml) and sandostatin (0.1 microg/ml) in vitro using the in situ terminal deoxynucleotidyl transferase assay (TDT) to detect apoptosis. The patients were female (mean age 54.4 years). In the 16 patients, 10 had positive estrogen receptor (ER) and positive progestogen receptor (PR), and the rest had negative ER and PR. RESULTS: 18 hours after addition of 5-FU, sandostatin and both, the apoptotic rate of tumor cells was (21.8 +/- 6.6)%, (14.3 +/- 8.8)%, and (26.7 +/- 6.9)% respectively. When samples were divided into 2 groups according to the sensitivity of ER and PR, the apoptotic rate of 5-FU plus sandostatin was (32.2 +/- 7.9)%, in positive ER and PR group, but (20.5 +/- 5.5)% in negative ER and PR group (P < 0.05). CONCLUSIONS: 5-FU plus sandostatin is more sensitive in the positive ER and PR group than in the negative ER and PR group. 5-FU plus sandostatin would be helpful in the biotherapy of breast cancer patients with positive ER and PR.

Adult↗
PubMed1999

[A study on expressions of P53 protein and proliferating cell nuclear antigen in malignant eyelid tumors].

OBJECTIVE: To observe the relationship between P53 gene mutation and malignant eyelid tumor. METHODS: The expressions of P53 protein and proliferating cell nuclear antigen (PCNA) in 102 cases with malignant eyelid tumors were examined by labelled streptravidin biotin (LSAB) immunohistochemical technique. RESULTS: Although overexpression of P53 protein was not detected in normal eyelid cutaneous epithelium, meibomian gland and mild epithelial dysplasia, the positive rate of P53 increased in degree in moderate and severe dysplasia, carcinoma in situ and invasive tumors, and PCNA labeling index (PI) progressively increased too. Overexpression of P53 protein was detected in 30/53 cases of basal cell carcinoma (BCC), 16/32 cases of squamous cell carcinoma (SCC), 10/17 cases of meibomian gland carcinoma, and the intensity of P53 protein immunostaining increased with the lowering in the degree of histological differentiation. The mean PCNA labeling index was 38.1 +/- 31.1, 38.3 +/- 30.1, 42.6 +/- 32.0 respectively in BCC, SCC and meibomian gland carcinoma. The PI value in tumor tissue with positive P53 protein expression was higher than that in tissues with its negative expression (P < 0.05). CONCLUSIONS: The gene mutation of P53 protein might play an important role in the pathogenesis of malignant eyelid tumors, it may be one of the factors that can lead to abnormal hyperactivity of cell proliferation and it possibly can be a malignant sign in the progression of a tumor.

Aged↗
PubMed1999

[The effect of extracting lens on damage of corneal endothelium in vitreous surgery].

OBJECTIVE: To observe the effect of extracting lens on the damage of corneal endothelium in vitreous and retinal surgery. METHODS: The corneal endothelial cell densities of 179 eyes were observed preoperatively and at postoperative 2, 4, 8 weeks and 3 and 6 months. RESULTS: Postoperatively, there was no significant change of the corneal endothelial cell densities compared with that of the preoperative ones in phakic eyes among different operations. There was also no significant change of the cell densities, but an tendency of cell loss in the aphakic and pseudophakic eyes after vitreous and retinal surgery. The corneal endothelial cell densities decreased significantly after vitrectomy with lensectomy. CONCLUSIONS: It is the vitrectomy with lensectomy that makes the corneal endothelial damage. Vitreous and retinal surgery poses a risk to corneal endothelium in the aphakic and pseudophakic eyes.

Adolescent↗
PubMed1999

[Analysis of MRI and CT manifestations of paranasal sinuses and orbitocranial disorders with secondary exophthalmos].

OBJECTIVE: To study the MRI and CT manifestations of paranasal sinuses and orbitocranial disorders with secondary exophthalmos. METHODS: MRI and CT scans of 51 patients were examined. RESULTS: MRI offers more information for evaluation of soft tissue lesions; the cavernous hemangioma and dermoid cyst were found to present rather characteristic images. Whereas in evaluation of lesions involving bony structures and calcifications, CT was superior to MRI. CONCLUSION: CT and MRI may evaluate the tumor location, extension into adjacent areas and are valuable in differential diagnosis of paranasal sinuses and orbitocranial disorder with secondary exophthalmos.

Adolescent↗