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Biomedical subjects

H Wagner

Publications and source records attributed to H Wagner.

At least 649 records · Page 36Linked to original sources
PubMed1980

T cell-mediated cytotoxic immune responsiveness of chimeric mice bearing a thymus graft fully allogeneic to the graft of lymphoid stem cells.

Fully allogeneic, chimeric mice were established by adult thymectomy of (A x B) F1 animals, grafting parental A-type thymus under the kidney capsula, followed by lethal (900 rd) irradiation and reconstitution with B parental-type bone marrow cells treated with xenogeneic anti-T cell antiserum plus complement. Following in vivo sensitization with inactivated Sendai virus (SV) suspensions, no virus-specific T cells could be detected within the spleen cells of the mice. Upon stimulation with third-party allogenic cells in a primary mixed lymphocyte culture, spleen cells of all animals generated alloreactive cytotoxic T lymphocytes (CLT). More interestingly, upon secondary in vitro stimulation with inactivated SV-conjugated B-type stimulator cells, B-type-restricted, virus-specific CTL were inducible in each case. Upon restimulation with SV-conjugated A-type stimulator cells, A being the H-2 type of the grafted thymus, T cells of some but no all mice generated A-type-restricted, virus-specific CTL. The data suggest that in allogeneic, chimeric mice virus-specific CTL can be induced. Moreover, virus-specific CTL, restricted to the H-2 type of the lymphoid stem cell inoculum, are more readily inducible than those restricted to the H-2 type of the allogeneic thymus.

Animals↗
PubMed1980

T-T cell interactions during in vitro cytotoxic T lymphocyte responses. III. Antigen-specific T helper cells release nonspecific mediator(s) able to help induction of H-2-restricted cytotoxic T lymphocyte responses across cell-impermeable membranes.

T helper cell induction and the specificity of T cell-mediated help as generated during alloreactive and H-2-restricted, virus- or hapten-specific cytotoxic T lymphocyte (CTL) responses have been compared. With the use of a double-chamber culture system, it was possible to dissect and separately analyze the induction phase of T helper cells from the T helper cell effector function. The data obtained revealed that during alloreactive as well as H-2-restricted T cell responses, antigen-specific T helper cells are induced. Upon specific restimulation of T helper cells, helper cell function is mediated across a cell-impermeable membrane via soluble products in an apparently nonspecific and nonrestricted manner. The data suggest that similar rules govern T-T cell interactions in alloreactive and H-2-restricted CTL responses.

Animals↗
PubMed1980

T cell factor (interleukin 2) allows in vivo induction of T helper cells against heterologous erythrocytes in athymic (nu/nu) mice.

Mice carrying the nude mutation (nu/nu) lack a functioning thymus and do not contain detectable levels of immunocompetent T cells. We now report that nu/nu mice to have lymphocytes which can be activated in vivo by heterologous erythrocytes and a Lyt-1 T cell-derived factor (interleukin 2) to generate T helper cells. Thus, a lymphokine is described which is able to restore in vivo T helper cell immunocompetence of nu/nu mice. The data may suggest that nu/nu mice contain a low number of T lymphocytes influenced by the cystic remnant of the nu/nu thymus anlage. Alternatively, the data imply that interleukin 2 circumvents the requirement of a thymus during ontogeny of T lymphocytes.

Animals↗
PubMed1980

T cell mediated cytotoxic immune reactivity of bone marrow reconstituted chimeric mice.

The capacity of bone marrow reconstituted chimeric mice to mount sendai virus specific H-2 restricted as well as alloreactive cytotoxic T-cell responses has been investigated. Chimeras of the type a leads to (a x b)F1 and (a x b)F1 leads to a were used. In addition chimeras produced with (a x b)F1 mice grafted under the kidney capsula with a type parental thymus, thymectomised, lethally irradiated and reconstituted with either a, (a x b)F1 or b type bone marrow cells were tested. In agreement with the data of Zinkernagel and Bevan it was noted that virus specific T cells of semi-allogeneic chimeric mice are able to recognise virus antigens in the context of the MHC of the thymus in which they have maturated. The unexpected finding was that fully allogeneic chimeras were able to mount both alloreactive CTL and H-2 restricted, virus specific CTL. The H-2 restricted CTL detected were preferentially restricted to their own MHC type. In some animals also virus specific CTL restricted to the allogeneic thymus MHC type were observed. Data are also presented to indicate that longterm cultured bone marrow cells have the potential to repopulate lethally irradiated mice.

Animals↗
PubMed1980

Herpes-Simplex-virus-specific, H-2Dk-restricted T lymphocytes bear receptors for H-2Dd alloantigen.

Cytotoxic T lymphocytes generated in the course of an HSV-infection of CBA (H-2k) mice not only lyse syngeneic, virus-infected target cells but also cross-react with noninfected taraget cells expressing the Dd alloantigen. On the effector cell level, this alloreactivity is mediated by virus-specific CTL's that are restricted to H-2Dk determinants. On the prekiller cell level, the anti-HSV-reactive T cells exhibiting cross-reactivity for Dd alloantigen could be positively selected on H-2d spleen-cell monolayers. After differentiation into cytolytic effector cells, target cells expressing Dd alloantigens and syngeneic HSV-infected target were lysed with equal efficiency. The results imply that the phenomenon of H-2-restricted versus nonrestricted T-cell reactivity is not due to distinct T-cell subsets, but rather is dependent on the antigeneic determinants recognized.

Animals↗
PubMed1980

[Results of reduction mammoplasties (author's transl)].

Report about the results of 170 reduction mammoplasties of which 40 were operated by the Strömbeck technique and 130 by a technique described by the authors of this report. The most frequent complication was blood loss. Using our operation technique we have observed only 4 partial necrosis (1,2%) of the areola, but no complete slough. The results of a questionnaire action confirmed the objective findings that reduction of a breast exceeding 600 ccm has to be considered a medical indication.

Adolescent↗
PubMed1980

H-2 restriction as a consequence of intentional priming: T cells of fully allogeneic chimeric mice as well as of normal mice respond to foreign antigens in the context of H-2 determinants not encountered on thymic epithelial cells.

Fully allogeneic chimeras were able to develop in vitro alloantigen-specific, as well as H-2-restricted, Sendai virus-specific cytotoxic T-lymphocyte (CTL) response. Depending on the immunization regimen used, Sendai virus-specific CTL responses were restricted to the H-2 antigens of either the stem cell donor or the thymus. Similarly, unprimed splenic T cells of normal mice were found to contain CTL-precursor cells that specifically reacted against Sendai virus or trinitrophenyl derivatives in the context of allogeneic major histocompatibility complex determinants that had not been encountered during their thymic differentiation. A frequency analysis of allogeneically versus syngeneically restricted virus-specific CTL precursors present in splenic T cells showed a ratio of about 1 to 6. These results provide evidence that H-2 restriction of trinitrophenyl- or Sendai virus-specific T cells is dictated by the complex type of the antigen-presenting cell and thus appears to be independent of the type of thymus in which the T cells have undergone maturation.

Animals↗
PubMed1980

Fine specificity and cytolytic activity of continuously growing alloreactive cytotoxic T lymphocyte clones.

The role of Lyt 1+ T-cell-derived secondard CTL inducing factor allowed the cloning of alloreactive cytotoxic T lymphocytes (CTL) by the limiting dilution approach. Several monoclonal cell lines were established in vitro. The lytic activity of some of the cell lines exceeded that of CTL from bulk cultures; that is, 50% of the target cells were lysed at an effector to target cell ratio of 0.04:1. The fine specificity of individual CTL clones is discussed.

Animals↗
PubMed1980

Different endocrinal and hemodynamic response patterns to various noxious stimuli.

Various endocrinal and hemodynamic parameters of 5 patients during a cardiovascular investigation were assessed in and after the adaptation phase, and together with the following noxious stimuli in random order: reading a confusion color chart based on the principle of interference in color perception, cold pressure test, isometric stress with a hand ergometer. The following parameters were assessed: arginine vasopressin, renin, heart rate, systolic, diastolic and mean arterial blood pressure, cardiac index, stroke volume and total peripheral resistance in the adaptation phase and during the various psychological and physical demand situations as well as 5 min afterwards. The results show that the organism reacts to the various stimuli with endocrinal and hemodynamic responses in a differentiated pattern in contrast to the model of the "alarm reaction" which, according to Cannon, states a homogeneous directed response with regard to the various parameters.

Adolescent↗
PubMed1980

[Somatostatin in the treatment of acute experimental pancreatitis (author's transl)].

An acute pancreatitis was induced in 20 Göttinger mini pigs by implantation of a T-tube and application of contrast medium. This process was demonstrated by laboratory and microscopic findings. Continuous infusion (n=11) of 2500 mcg somatostatin (GHIF) over 10 h showed a significant reduction of amylase and blood glucose level corresponding to the microscopic findings. The group of animals treated with somatostatin (GHIF) showed a ratio of 1:5 between necrotic and unaffected parenchymal tissues. By contrast, the histologic findings of the untreated group showed a ratio of 1:1. The investigations of blood glucose, gastrin and glucagon levels showed a rebound effect after ending the treatment with somatostatin.

Acute Disease↗
PubMed1980

[Secretion of prolactin in healthy men and women of different age (author's transl)].

In 50 healthy women and 57 healthy men aged 20--80 years serum-prolactin-levels before and after intravenous injection of 200 micrograms TRH were measured and correlations with serum-triglycerid-, sodium-, basal TSH and serum insulin levels as well as sodium concentration of red blood cells, age, relativ body weight (Broca) and blood pressure were calculated. In men of different age no changes in basal serum-prolactin-levels can be detected. In 60--80 years old men a significant higher but later occuring TRH-induced prolactin secretion than in younger men can be found. 50--59 year old women have a significant higher prolactin secretion after TRH application than 20--29 year old females. Basal serum prolactin levels of women correlate negative significant with serum sodium levels and positive significant with the sodium concentration of red blood cells and basal TSH values. Relative prolactin out-put (prolactin-max--prolactin-basal) after TRH-application in women is found to correlate positiv significant with serum triglycerid levels. In men relative prolactin out-put following TRH-injection correlates negativ significant with basal serum insulin levels. This correlation diminishes when age and Broca are kept constant in the calculation.

Adult↗