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Biomedical subjects

H Wagner

Publications and source records attributed to H Wagner.

At least 631 records · Page 35Linked to original sources
PubMed1981

[Long term experience with the IUD copper-T-200 (author's transl)].

During a period of seven years pregnancy-, expulsion- and extraction-rates of the copper-T-200 were determined for two different groups of patients in a university hospital. The first group were patients of the ambulatory care service representing an heterogenous composition. The other group were patients from a private practice representing a more homogenous population. In the first group insertions and controls were done by different physicians at various stages of their training, in the second group they were performed by an experienced clinician. In the first group there were 1397 primary IUD-insertions and 22,203 months of use, in the second group the corresponding data were 272 primary insertions an 5127 months of use. The pregnancy rates calculated according to the Pearl-index were 1.0 and 0.24, respectively. The expulsion rates were calculated as 0.8 and 0.2, respectively. The extraction rates because of unwanted side-effects were 4.4 in the first and 1.6 in the second group of patients. There seems to be an advantage in having the care of the IUD-patient being taken by an experienced physician, especially regarding the extraction rates because of unwanted side effects.

Female↗
PubMed1981

Reactivity in the MEM-test of cancer patients' lymphocytes incubated with brain-derived preparations and extracts from malignant teratomas.

A batch of human encephalitogenic protein (HEP) was compared to preparations of microtubulin and associated proteins (MST) from guinea pig brain by serological techniques and the macrophage electrophoretic mobility (MEM) method. HEP consisted mainly of A1 protein and MST showed the characteristic double band in the 55,000 MW region and some additional weak protein bands. No cross-reactivity could be detected between HEP and MST by rabbit hyperimmune sera to these substances. The MEM test, however, revealed human lymphocyte reactivity to HEP and MST in both cancer and multiple sclerosis patients, but no remarkable responses in other patients. A synthetic nonapeptide (114-122 region of A1 protein) led to lymphokine release in 2 of 5 multiple sclerosis patients, in 1 of 13 cancer patients, and in none out of 13 control individuals. From these findings it is concluded that a minor component (contaminant?) present in both HEP and MST might induce lymphocyte responses in cancer patients. The A1 protein of HEP preparations is, if at all, not the only protein responsible for the so-called HEP-response of cancer patients. Continuing the MEM test experiments with extracts from single organ cancers, it could be shown that patients with malignant teratomas respond to a more or less broad spectrum of extracts bearing organ cancer specificity. On the other side, extracts made from 2 malignant teratoma tissues led to MEM responses in 8 of 13 cancers, in 2 of 11 control persons and in none of 5 multiple sclerosis patients. These findings underline the concept that tumour-associated antigens (possibly oncofetal antigens) do exist, which bear organ cancer specificity and will lead to a specific sensitization of the host. Such antigens may be expressed in teratomas in different qualities and amounts depending on the tissue differentiation capacity of a given teratoma.

Cross Reactions↗
PubMed1981

[Determination of triiodothyronine in serum with a heterologous enzyme immunoassay: results of a group survey].

This paper summarises the results of the evaluation of a heterologous enzyme immunoassay in antibody coated tubes for the determination of triiodothyronine (T3) by a group of 11 laboratories. The assay procedure is analogous to an established heterologous thyroxine enzyme immunoassay. The evaluation demonstrated that 1. the specificity of the assay and its analytical range between 0.46 and 9.2 nmol/l meet diagnostic requirements, 2. the intra- and interassay precision was in accordance with that commonly found in radio immunoassays, 3. the recovery of added T3 was between 90 and 103%, 4. the results agreed well with those of self-established radio immunoassays and were comparable with commercial RIA kits, 5. there was no interference by hyperbilirubinaemia or by high concentrations of bile acids, but interference can occur in very haemolytic and lipaemic samples. The great advantage of T3 enzyme immunoassays lies in the absence of restrictions and any authorization needed for working with radioactive substances.

Evaluation Studies as Topic↗
PubMed1981

Preliminary characterization of a colon tumour tissue preparation used in macrophage electrophoretic mobility (MEM) test1.

A 3M KC1-extract on nine samples of colon carcinomas was freed from human serum proteins by immunoadsorption. Both the KC1-extract and the immunoadsorbed preparation of this KC1-extract were used in MEM test and gave characteristic reaction patterns. The two preparations were compared and preliminarily characterized by immunological and electrophoretical methods.

Antigens, Neoplasm↗
PubMed1981

A test-subject-tracking measuring carriage with optoelectronic position-feedback control for the kinematic analysis of the gait of orthopedic patients.

A measuring carriage system has been developed in our biomedical motion-analysis laboratory for the continuous optoelectronic measurement of angular coordinates or point coordinates in the sagittal plane of a walking patient. The carriage is automatically driven on a guide rail in parallel with the advancing test subject along a level walkway 14 m in length. The carriage propulsion is effected by a servomotor which is controlled by an object-tracking optoelectronic position-feedback control system. The paper describes the constructional principles of the system and gives a basic analysis of its feedback-control behavior.

Biomechanical Phenomena↗
PubMed1980

Alloreactive and H-2-restricted Lyt 23 cytotoxic T lymphocytes derive from a common pool of antecedent Lyt 123 precursors.

If the collaborative requirement of Lyt 1 T helper cells is bypassed by the Lyt 1 T cell-derived mediator of T help, termed Il-2, upon antigenic stimulation, PNA+ Lyt 123 thymocytes differentiate into either alloreactive or H-2-restricted PNA- Lyt 23 cytotoxic effector cells. Along the differentiation pathway from Lyt 123 leads to 23 effector cells, cytolytic activity is carried out by T cells that still express the Lyt 123 phenotype. The data establish that Lyt 23 CTL are produced by differentiation from antecedent Lyt 123 cells.

Animals↗
PubMed1980

Translation of poly-A RNA from rat liver in vitro. Evidence for a high molecular weight subunit of tyrosine aminotransferase.

Poly-A RNA extracted from the rat liver was translated in a cell-free wheat germ system and a rabbit reticulocyte lysate. The subunit of tryptophan pyrrolase precipitated by specific antiserum after synthesis in vitro has the same molecular weight as the corresponding subunit derived from the rat liver. With specific antiserum prepared against tyrosine aminotransferase, however, a radioactive protein from both the in vitro assays was precipitated with an about 5% higher molecular weight than the tyrosine aminotransferase subunit precipitated from rat liver. The immunological evidence and the comparison of the specific peptide patterns prepared by cyanogen bromide treatment showed that the in vitro product corresponds to tyrosine aminotransferase. Various concentrations of potassium or spermidine used in the wheat germ translation system did not alter the size of the enzyme subunit synthesized. The run of the tyrosine aminotransferase purified form the rat liver in the SDS-polyacrylamide gel electrophoresis was not influenced by treatment with Escherichia coli alkaline phosphatase. The possibility is discussed that the larger enzyme synthesized in vitro represents a precursor molecule which is cleaved proteolytically in vivo.

Animals↗
PubMed1980

[Changes in the immunological components of the blood during balneotherapy in patients with rheumatoid arthritis].

The changes of the immunoglobulin G, the C-reactive protein and the acid alpha 1-glycoprotein which were observed under spa therapy in patients with rheumatoid arthritis. In these cases was shown that these parameters undergo regulative influences which in typical relationship to the initial tissue lead to a cross-over-phenomenon with demarcation of a regulatory zero- (or normal) region. Here patients with rheumatoid arthritis show the tendency to maintain a slightly increased defensive condition under the cure. On the basis of a narrowing of the dispersion of the immune components to the end of the cure compared with the beginning of the cure is concluded that the changes of defensive components to the blood which are to be observed in different initial values are not only based on the endogenic rhythmicity but partly also on the effects of the spa therapy.

Arthritis, Rheumatoid↗