Biomedical subjects
H Wada
Publications and source records attributed to H Wada.
Capillary hemangioma of the optic disc.
Two cases of capillary hemangioma of the optic disc (one endophytic and the other exophytic) unassociated with central nervous system involvement are reported. Ophthalmoscopic and fluorescein angiographic findings of capillary hemangiomas of the optic disc are summarized to aid in differentiating them from cavernous and racemose hemangiomas. Therapeutic problems are also discussed.
Mammalian histidine decarboxylase and its suicide substrate alpha-fluoromethylhistidine.
Explore the source record for details and available documents.
Electron microscopic identification of histidine decarboxylase-containing endocrine cells of the rat gastric mucosa. An immunohistochemical analysis.
The localization of histidine decarboxylase-like immunoreactive structures in the mucosal cells of the rat stomach was studied by the peroxidase-antiperoxidase method. At the light microscopic level, histidine decarboxylase-like immunoreactivity-containing cells were concentrated in the basal part of the oxyntic region, whereas in other areas no immunoreactive cells were seen. Ultrastructural study showed that reaction end products were diffusely distributed within the cytoplasm of the enterochromaffinlike cells but other cell types such as A cells, G cells, and enterochromaffin cells were not labeled. These findings suggest that enterochromaffinlike cells synthesize histamine.
[Segmented polyurethane functional removable denture, the "Biotron-P"].
Explore the source record for details and available documents.
[Evaluation of true titanium cast crowns for clinical use].
Explore the source record for details and available documents.
Possible descending pathways mediating spontaneous tremor in monkeys.
Explore the source record for details and available documents.
[Effect of ketogenic diet on the convulsive threshold and brain amino acid and monoamine levels in young mice].
Explore the source record for details and available documents.
[Immunological studies on glomerulonephritis and nephrotic syndrome].
Explore the source record for details and available documents.
[Recent trend of tuberculosis and the results of treatment in the area around Shimada city].
Explore the source record for details and available documents.
[Pleuropneumonectomy for the treatment of chronic thoracic empyema].
Explore the source record for details and available documents.
[A case of a congenital left pericardial defect associated with bilateral bullous emphysema].
Explore the source record for details and available documents.
[A case of pulmonary alveolar proteinosis associated with pulmonary tuberculosis].
Explore the source record for details and available documents.
[Preparation of repeatedly and effectively usable L-asparaginase by a chemical modification].
The anti-tumor activity of L-asparaginase (EC. 3.5.1.1.) has been conclusively demonstrated. Its therapeutic application, however, has been hampered by its short clearance time in the circulation and high immunogenicity. In order to solve these problems, polyethylene glycol, a linear synthetic and non-immunogenic polymer, was introduced covalently into the amino groups in the enzyme molecule. Monomethoxypolyethylene glycol with molecular weight of 5000 was activated with cyanuric chloride to obtain activated PEGs [2, 4-bis (O-methoxypolyethyleneglycol)-6-chloro-s-triazine]. L-Asparaginase lost its immunoreactivity against its antibodies after the modification of 52 out of 92 amino groups in the molecule. This modified asparaginase retained 11% of its enzymic activity under the physiological condition. When the modified asparaginase was administered in rodents, it diminished the serum asparagine level and its enzymic activity persisted in the circulation 10 to 20 times longer than that of native enzyme. Furthermore, repeated injections of modified asparaginase did not induce any significant anti-asparaginase antibody production. Modified asparaginase showed a superior anti-tumor activity to the native counterpart irrespective of the presence of anti-asparaginase antibodies, when it was tested with murine Gardner lymphoma. This chemical modification should make it possible for the first time to repeatedly and effectively use L-asparaginase obtained from a bacterium.
[Prognostic significance of intraventricular hematoma in the cases of intracranial hemorrhage].
Fifty two patients with intracranial hemorrhage, which was diagnosed with CT scan, were reviewed in relation to the prognostic significance of the intraventricular hematoma. Following items were analyzed in each patient in order to classify the severity of the patients; cause of the hemorrhage, level of the consciousness at the time of evaluation, volume of the intraventricular hematoma, midline shift, cast formation of the intraventricular clots in the third and/or fourth ventricle and acute hydrocephalus. The total mortality rate of the patients analysed was 32.7%, whereas 42.3% of the patients returned to useful life. Level of the consciousness and cause of the hemorrhage were found most contributory factors to the prognosis, and, in general, the patients whose consciousness were better than in semicomatose state, could expect reasonably fair prognosis. When the level of consciousness was worse than semicoma, they were divided in two groups according to the cause of hemorrhage. Cases with ruptured intracranial aneurysm or hypertensive hemorrhage showed as a rule poor prognosis and, on the contrary, patients with AVM or MoyaMoya disease were not always poor in outcome. In the former group the level of consciousness was quite low and the prognosis was poor with higher mortality, if the intraventricular clot occupied over two third of the ventricles. However, cases with intraventricular clot caused by AVM stayed in good level of consciousness even the intraventricular hematoma extended into the all ventricles, and the prognosis was not pessimistic unless the AVM was unoperable. The midline shift on CT scan was well correlated with the high mortality in the cases of ruptured aneurysm.(ABSTRACT TRUNCATED AT 250 WORDS)
Radioimmunoassay for human pancreatic ribonuclease and measurement of serum immunoreactive pancreatic ribonuclease in patients with malignant tumors.
A method for radioimmunoassay of human pancreatic RNase was developed. The method is sensitive, reproducible, and specific. Almost no cross-reactivity exists between human pancreatic and liver RNases. A good correlation was observed between the serum concentration of pancreatic RNase as measured by radioimmunoassay and its enzymatic activity using polycytidylic acid as substrate. The concentration of serum pancreatic RNase correlates well with age, blood urea nitrogen, and albumin contents but does not correlate with serum amylase activity. Using the data of 52 patients with malignant tumors except pancreatic cancer, serum RNase level could be expressed by a multiple regression equation: Immunoreactive RNase content in pancreatic cancer was elevated in patients with complications from renal failure. Serum pancreatic RNase contents in patients with pancreatic cancer measured by radioimmunoassay agreed well with the values calculated using the equation derived from the data of patients with other malignant tumors.
Intracellular localization of glutamic oxaloacetic transaminase isozymes.
Explore the source record for details and available documents.
[Effect of aging on blood catecholamine under 3 conditions of sodium intake--comparison between normal subjects and patients with essential hypertension].
Increased sympathetic nervous activity may play an important role in the pathogenesis of essential hypertension. It is well-known that both dietary sodium intake and age influence plasma norepinephrine (P-NE). There are many reports on comparative studies of P-NE in patients with essential hypertension and normotensive controls. However, there are few studies on changes in plasma catecholamines in both the aging and salt intake states. The present study was undertaken to evaluate the effect of age on sympathetic nervous activity in patients with essential hypertension and normal controls under low (UNa V 29 +/- 3 mEq/day), regular (UNa V 133 +/- 8 mEq/day) and high (UNa V 324 +/- 12 mEq/day) sodium regimens. The catecholamine levels were analyzed by THI methods after HPLC separation. Twenty-four hour urinary norepinephrine, epinephrine and electrolyte (Na+, K+) excretion on day 5 of each regimen was also determined. On day 6 morning, supine and upright P-NE, plasma epinephrine and plasma renin activity were determined after blood pressure and pulse rate measurement. Subjects were separated according to age as follows: young (less than 40 y.o.), middle-aged (40 approximately 60 y.o.) and old (greater than 60 y.o.). P-NE in hypertensives was significantly higher than in normals under any three sodium intakes. There was a significantly positive correlation between age and P-NE, but only in normal controls under three sodium intakes and not in hypertensive patients under any three sodium regimens. In the young group of hypertensive patients, P-NE was significantly higher than that of normal controls. These results suggest that the increased sympathetic nervous activity plays an important role in the pathogenesis and maintenance of essential hypertension, and this is particularly obvious in young patients.