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Biomedical subjects

H W Doerr

Publications and source records attributed to H W Doerr.

At least 217 records · Page 12Linked to original sources
PubMed1982

Rapid separation of immunoglobulin M by immunoaffinity chromatography for detection of specific antibodies to rubella and Treponema pallidum.

A rapid and easy method for isolating IgM from serum specimens in order to detect specific antibodies against Treponema pallidum and rubella virus by routine serologic procedures is described. Serum IgM was isolated by immunoaffinity chromatography using anti-human IgM antibodies covalently bound to controlled-pore glass beads in a microcolumn. The final concentration of the IgM in the samples tested amounted to at least 16% (average 32%) of the original concentration (corresponding to a serum dilution of 1 : less than 8). IgG contamination did not exceed 0.38% of the original serum concentration. The capacity of the column was stable for at least 50 absorption/elution cycles. The new technique enables rapid and reliable detection of specific IgM by the rubella hemagglutination inhibition and Treponema pallidum hemagglutination tests.

Animals↗
PubMed1982

Hepatitis A and B markers and presumable non-A, non-B hepatitis in a psychiatric institution.

The frequencies of hepatitis A and hepatitis B markers were determined in 291 members of the staff and 714 patients of a psychiatric institution for adults. 71.8% of the patients suffered from psychosis, 17.7% from neurosis, and 10.6% from oligophrenia. 84.4% of the patients and 69.2% of the staff were anti-HAV-positive. - 26.8% of the patients and 19.9% of the staff showed at least one HBV marker. The frequency of anti-HBc increased with age but not with hospitalization. Anti-HBc-positive persons did not show transaminase abnormalities more often as reported previously. - 2.6% of the patients were presumable Non-A, Non-B hepatitis cases after excluding IgM-anti-HAV, IgM-anti-CMV or -EBV and possible toxic effects. The rate of presumable Non-A, Non-B hepatitis equaled the frequency of HBsAg-positive persons.

Adult↗
PubMed1982

Presumable non-A, non-B hepatitis in hemodialysis.

Hepatitis A and B markers, IgM-anti-CMV and -EBV were determined in 154 dialysis patients, 118 relatives of patients and 42 members of the staff. Episodes of elevated transaminases were investigated for presumable non-A, non-B hepatitis as well as secondary infections among relatives. --The cumulative frequency of HBV markers was 71% in center dialysis patients, 63% in home dialysis patients, 50% in staff and 19% in relatives. The frequency of HBV markers increased with duration of dialysis treatment and with the number of blood units transfused. Spouses of dialysis patients had more often HBV markers than other relatives (22% vs. 8.8%). 96.8% of the center dialysis patients, 83.3% of home-dialysis patients, 41% of the staff, and 42% of the relatives were anti-HAV-positive. The incidence of IgM-anti-CMV was highest in home-dialysis patients (15.5%). The frequency of IgM-anti-EBV was 4.3% in center-dialysis patients and 12.7% in home-dialysis patients. --9% of all dialysis patients had an episode of elevated transaminases compatible with non-A, non-B hepatitis. The possible secondary attack rate among relatives was 31%.

Antibodies, Viral↗
PubMed1982

Rapid detection of Treponema pallidum and cytomegalovirus specific IgM antibodies with the passive haemagglutination.

For the detection of Treponema pallidum-specific IgM antibodies in patients' serum samples four simple and rapid methods are compared and evaluated. They show decreasing sensitivity and specificity in the row listed: (1) Treponema pallidum haemagglutination (TPHA) after IgM separation by affinity chromatography on controlled-pore glass heads (anti-IgM-CPG), or (2) by ion exchange chromatography on DEAE-cellulose; (3) solid-phase haemagglutination (SPHA) in glass microvials or (4) on polysterene microplates. The latter tests have found to be inferior, although they can be considerably improved by the use of affinity chromatography--purified catch antibodies. The commercially available DEAE-cellulose microcolumn delivers a simple method for IgM separation, and in spite of a relatively high IgA contamination it proved to be useful also for CMV-specific IgM haemagglutination tests, as compared to ELISA results.

Antibodies, Bacterial↗
PubMed1982

Comparison of different methods for assessment of rubella infection and immunity.

The different enzyme-linked immunosorbent assays (ELISA), a fluorescence immunoassay (FIAX), and a hemolysis-in-gel test (HIG) were examined for sensitive detection of specific antibodies in sera of persons with acute and previous rubella infection. In comparison to hemagglutination inhibition (HI), all tests were able to detect previously formed rubella antibodies. In sera of patients with acute rubella infection, only the FIAX system showed always positive results. Nevertheless, it remains unclear, if these findings are due to a higher sensitivity of the FIAX system or are possible non-specific results. In some cases, however, this test system failed to detect significant titre rises seen in HI, because it showed high antibody titres already in the first serum sample.

Antibodies, Viral↗
PubMed1981

[Q fever (author's transl)].

Comparative analysis of two Q fever epidemics in Baden-Württemberg demonstrated the following characteristics of this zoo-anthroponosis endemic in southern Germany: 1. affected areas are situated along sheep tracks and natural concentrations of the Dermacentor marginatus. 2. The disease spreads in waves. 3. It causes typical symptoms, particular an intense tension headache, and typical radiographic findings. Men are more commonly and more severely affected than women. Children are not affected despite proven exposure. 4. The CBR antibody titre is a reliable laboratory diagnostic parameter. 5. Epidemiological measures (search for the source of infection, elimination of seropositive sheep, use of disinfectants) reduce the reservoir of the infective agent. 6. The therapeutic drug of choice is doxycyclin.

Adolescent↗
PubMed1981

Comparison of different methods for the detection of rubella-specific IgM antibodies.

The rubella specific IgM titer in the serum specimens originating from healthy persons and from patients with clinical signs of rubella infection was determined by hemagglutination inhibition or hemagglutination reduction after IgM separation with the following methods: (a) density gradient centrifugation; (b) polyacrylamide agarose gel chromatography; (c) ion exchange chromatography with diethylaminoethyl cellulose columns; (d) solid-phase immunosorbent technique using microplates; (e) solid-phase immunosorbent technique using polyacrylamide microimmunobeads. Alternatively, we removed IgG and IgA by the use of protein A, anti-IgG, and anti-IgA, covalently coupled to controlled-pore glass (f). The titers obtained by the different methods showed qualitatively good correlations when combined with mercaptoethanol reduction. The quantitative measurement of specific IgM titers, however, revealed a lower sensitivity of column chromatography and methods of removal of IgG/IgA.

Antibodies, Viral↗
PubMed1981

Rapid detection of rubella-specific IgM antibodies by the use of microimmunobeads (MIB-IgM).

A simple haemagglutination (HA) titre reduction assay for the detection of rubella-specific IgM antibodies has been developed. Serum IgM is rapidly isolated by immune adsorption to heavy chain-specific anti-human IgM antibodies covalently coupled to polyacrylamide microimmunobeads. The immobilized immunocomplexes are incubated with rubella virus HA antigen. Subsequently, the particles are centrifuged from the solution, and the reduction of the HA titre is determined. This procedure has proven to be as reliable as haemagglutination inhibition (HAI) tests carried out with IgM fractions separated on a sucrose density gradient.

Antibodies, Viral↗
PubMed1981

[Determinations of immunoglobulin from the blood of the umbilical vein after pathological course of pregnancy and in foetal growth retardation (author's transl)].

Serum-IgM and serum-IgA concentrations of 561 unselected samples of umbilical cord were determined by the radial immunodiffusion method (Mancini's technique). The mean IgM value of all blood samples was 13.8 mg%. In 9.9% of all cases only we found measurable IgA values, the average IgA concentration being 4.5 mg%. Elevated IgM values (greater than 30 mg%) - pointing to an intrauterine infection - were found to be more frequent (12.1%) in foetal of growth retardation than with newborn of normal body weight, where the incidence of increased IgM values was 5.5% of the examined cases only (however, without a statistically significant difference). In preceding infections during pregnancy, elevated IgM values (greater than 30 mg%) were also found more frequently than in cases without infections (11.8% vs. 5.7%, respectively). Basing on these results, we can suspect that intrauterine infection is one of the possible causes of foetal growth retardation, but not one of the main ones.

Adult↗
PubMed1980

[Virus-specific IgM proof with routine serologic methods (author's transl)].

By the use of a specific immunosorption to insoluble adsorbentia (controlled-pore glass, polystyrene particles) the separation of IgM and IgG is performed in serum specimens, which originate from patients presenting several virus infections (mumps, measles, cytomegalovirus, herpes simplex), for the virus-specific IgM proof with routine serologic methods (CFT, HIT, NT). The results are in a good agreement to them seen in the demonstration of significant titer rises to assure the diagnosis of acute mumps and measles infections by HIT rapidly. While the new technique is also successfully applied for the determination of neutralizing IgM and IgA antibodies to HSV, no sufficient results are available to detect CMV specific IgM antibodies by CFT compared to other methods (IFT, ELISA).

Adolescent↗
PubMed1979

[Immunity against poliomyelitis in the German Federal Republic (author's transl)].

The immunity of the populations of several countries of the GFR against poliovirus 1, 2 and 3 was investigated in 11 laboratories. Sera of 4707 persons aged 0 to 30 years were assessed for neutralising antibodies (serum dilution 1:4) against the 3 types of poliovirus. 34.5% of the 4707 investigated sera had no neutralising antibodies at least against one type of the poliovirus, 4.7% showed no antibodies against all 3 types of the virus. Especially children aged 0 to 4 years were protected incompletely against the 3 types of poliovirus. In comparison with similar investigations of 1969 and 1972 no decisive change of immunity of the population of the GFR against poliomyelitis has occurred.

Adolescent↗
PubMed1979

Simple method to detect virus-specific IgM antibodies in patients' serum samples after immunosorption of immunoglobulins G and A.

By absorption with protein A and specific immunosorption using insolubilized adsorbentia (CPG), a method was developed for rapid elimination of IgG and IgA from patients' serum samples. This test technique was examined in the diagnosis of rubella infections, i.e., rubella-specific IgM antibodies. The procedure has proven to be as reliable as determinations carried out with IgM fractions separated on a sucroe density gradient.

Antibodies, Viral↗
PubMed1979

Cytomegalovirus strain differentiation by DNA restriction analysis.

The heterogeneity of CMV DNA obtained from standard strains and new isolates, including a vaccination strain (Towne 125), was investigated. The cleavage patterns produced by the restriction endonucleases Eco RI and Bam 1 revealed stable strain specificities of CMV. On the other hand, a remarkable homology of sequence-specific CMV DNA fragmentation was demonstrated. A CMV subtyping relevant to clinical questions seems to be improbable.

Antigens, Viral↗
PubMed1977

[New aspects on the treatment of pneumonia associated with acute mycoplasma infections (author's transl)].

Over a period of 16 months acute mycoplasma pneumonia was diagnosed in 20 patients. All were rapidly and completely cured by ampicillin and cephalosporins, which are ineffective against mycoplasma, indicating that acute mycoplasma infection in children does not require selective treatment, the pneumonia probably being caused by other micro-organisms. There is no prove that mycoplasma pneumonia heals spontaneously, without any therapy.

Ampicillin↗
PubMed1977

IgM antibodies to cytomegalovirus during pregnancy.

IgM antibodies to cytomegalovirus (CMV) could be detected in about 7 per cent of 629 pregnant women whereas in 225 nonpregnant control women of similar age distribution only 2.6 per cent showed CMV IgM antibodies. Intrauterine CMV infections were almost exclusively detected among the CMV IgM positive gravides. The high incidence of CMV IgM antibodies in pregnant women can be possibly explained by an increased rate of CMV reactivations during pregnancy. We were able to show that during CMV reactivation an intrauterine infection might occur.

Antibodies, Viral↗