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Biomedical subjects

H Uchida

Publications and source records attributed to H Uchida.

At least 577 records · Page 32Linked to original sources
PubMed1985

Antitumor activity of aqueous extracts of marine animals.

Aqueous extracts (macromolecule fractions in particular) of 25 species of well-known marine animals were tested for their antitumor activity against transplanted sarcoma 180 solid form in ICR mice. All of the macromolecule fractions from the extracts of animals in the shellfish category except that of Mytilus edulis inhibited the growth of sarcoma 180. The use of aqueous extracts from Strongylocentrotus nudus, Halocynthia hilgendorfi f. ritteri, Styela plicata, Ecteinascidia turbinata, and Megabalanus rosa also revealed relatively high inhibition ratios of over 60%.

Animals↗
PubMed1985

Recurrent ectopic junctional tachycardia in Ebstein's anomaly. Case report of a 67-year-old man.

We reported the case of a 67-year-old man with Ebstein's anomaly, the oldest patient with this disease in Japan as far as we know. His condition was often accompanied by ectopic junctional tachycardia with isorhythmic AV dissociation, which made him complain of palpitation by elevating right intra-atrial pressure. Although enhanced automaticity was the most likely mechanism of this tachycardia, it was abruptly initiated and terminated by a single premature contraction. Because this interesting character can not be explained solely by the usual ideas of automaticity, we suggest that ectopic junctional tachycardia in the present case may somewhat resemble the phenomenon of triggered automaticity.

Aged↗
PubMed1985

[Long-term toxicological studies with mizoribine (Bredinin) in beagle dogs (the first report). Blood chemistries, pharmacokinetics of mizoribine and fertility studies of the male dogs].

For long-term toxicological studies, mizoribine at the dose of 2.5 mg/kg body weight was administered orally to two male and two female Beagle dogs for 36 consecutive months, once a day, 6 days a week, with a day-off on the 7th of each week. As a control group, 2 male and 2 female dogs were kept under the same conditions as the treated group. The above experiment resulted in no abnormal general symptoms being found in either group. In addition, body weight gain instead of weight loss was observed in both groups. Moreover, hematological and biological parameters were in the normal range, and no statistical difference was obtained between the two groups. An additional 10 mg/kg of mizoribine was administered once to both groups at the months of 6, 12, 18, 24, or 36 after the onset of this toxicological study so as to determine the pharmacokinetic behavior of serum mizoribine. This resulted in no significant difference being observed between the two groups. Therefore, it can be inferred that accumulation of mizoribine is not induced by its long-term repeated administration. In fertility study, the analysis of the sperm and the mating ability of a male dog with an appropriate healthy female dog revealed that mizoribine at this dose did not provoke any abnormalities in male gonadal functions.

Animals↗
PubMed1985

Increase of phosphotyrosine-containing proteins in human carcinomas.

Immunofluorescent (IF) staining with monoclonal antibody to O-phosphotyrosine (PTYR) has shown that a variety of human carcinomas (liver, esophagus, stomach, lung, colon and breast) have increased amounts of PTYR-containing proteins (PTYR-proteins), which are localized in cytoplasmic regions and nucleolus-like structures of the carcinoma cells. PTYR-proteins were isolated from carcinomas of the liver, stomach and esophagus, and also from normal regions of these organs by immunoaffinity chromatography with polyclonal antibodies to PTYR. They were then labeled with 125I and analyzed by gel electrophoresis. Twenty-eight species of PTYR-proteins in a molecular weight range from 310,000 to 23,000 were detected, of which the major 16 species were confirmed to contain 125I-labeled diiodo-O-phosphotyrosine residues. All the PTYR-proteins found in carcinomas were also present, though at much lower levels, in normal regions of the respective organs.

Carcinoma↗
PubMed1985

[Effective postoperative chemotherapy of gastric cancer associated with disseminated carcinomatosis of the bone marrow].

A 35-year-old woman tuffering from gastric cancer associated with disseminated carcinomatosis of the bone marrow is reported. Total gastrectomy combined with splenectomy and distal pancreatectomy was performed. The patient was treated with mitomycin C, FT-207, OK-432, and PSK. But serum ALP (alkaline phosphatase) and CEA (carcinoembryonic antigen) values showed gradual elevations followed by deterioration of the patient's general condition. Consequently, chemotherapy program consisting of 5-fluorouracil, Adriamycin (intra-arterially), and cisplatin (intravenously) was initiated. Serum CEA and ALP values were considerably improved, and patient was restored to a better condition. She survived 17 months and died of disseminated intravascular coagulation.

Adenocarcinoma↗
PubMed1985

[Comparative studies of antimicrobial agents against causative organisms isolated from urinary tract infections (1983). III. Secular changes in susceptibility].

The in vitro antimicrobial activities of antibiotics against causative pathogens isolated from patients with urinary tract infections (UTI) in the 8 institutions in Japan during the period from 1980 to 1983 were compared. A number of new beta-lactam antibiotics with broad spectrum of activity have become available for clinical use in recent years. Some of them, in particular so-called third generation cephems, are reported to be responsible for developing microbial-cross resistance to multiple beta-lactam antibiotics. We have been making survey in recent years to explore changes in susceptibility to various antimicrobial agents of clinical isolates. All bacterial isolates from clinical specimens were submitted to the Department of Clinical Pathology, Juntendo University School of Medicine, where they were tested for antimicrobial susceptibility with MIC 2000 apparatus. Of all pathogens from patients with simple UTI, the majority of the isolates was E. coli and Klebsiella spp. In cases of complicated UTI, on the other hand, Pseudomonas spp. were most frequent, followed in order by Proteus spp., Klebsiella spp., Serratia spp., Citrobacter spp. and E. coli. Conspicuous changes in antimicrobial activity of antibiotics against E. coli and Klebsiella spp. from simple UTI have not been found in our survey. Against strains of Citrobacter spp., even the third generation cephems proved to be not remarkably active and there was a significant decrease in susceptibility of isolates to the drugs test-showed MIC values of 50 micrograms/ml and the proportion increased to 50% (22/44) with isolates obtained in 1982. The antimicrobial activity of cefsulodin and gentamicin against P. aeruginosa was decreased in 1982 compared with that in 1980 and 1981. However, resistant strains were slightly more frequent with gentamicin. In 1983, the antimicrobial activity of third generation cephems against Serratia spp. was significantly reduced from that in 1982.

Anti-Bacterial Agents↗
PubMed1985

[Comparative studies of antimicrobial agents against causative organisms isolated from urinary tract infections (1983). I. Susceptibility distribution].

During 1979-1983 in vitro activities of antimicrobial agents against causative bacteria isolated from patients with urinary tract infections (UTI) were investigated by Microbiological Research Group of UTI including the 8 institutions in Japan. Of all strains (219) isolated from patients with simple UTI, 65.3% were E. coli, and 9.6% Klebsiella spp.; these species accounted for about 75% of all isolates in 1983. MPC and CCL among the oral antimicrobial agents have showed potent activities against E. coli, MPC at 1.56 micrograms/ml and CCL at 3.13 micrograms/ml inhibited 80% of E. coli from simple and complicated UTI. CTM, CTX, CZX, CMX and LMOX among the parenteral antimicrobial agents, at concentrations of 0.20 micrograms/ml or less inhibited 90% of E. coli isolated from simple and complicated UTI. The frequency of isolates from patients with complicated UTI, without catheter, was as follows; E. coli 27.6%, P. aeruginosa 20.1%, Streptococcus spp. 12.6%, Serratia spp. 8.8% and Klebsiella spp. 8.0%. The frequency of isolates from patients with complicated UTI, indwelling catheter, was as follows; P. aeruginosa 22.6%, Streptococcus spp. 18.0%, Serratia spp. 15.0%, Proteus spp. 12.4%, and Enterobacter spp. and Klebsiella spp. are 6.0%, respectively, in 1983. The antibacterial activity (MIC80) against E. coli, Klebsiella spp., P. mirabilis, Citrobacter spp. and Serratia marcescens from simple and complicated UTI was compared, for example, among third generation cephem antibiotics. Four drugs such as CMX, CZX, CTX and LMOX showed virtually comparable activities while CPZ was slightly less active against the strains tested. There have been many studies reported concerning the antibacterial activity of various drugs against clinical isolates from patients with infections, but it seems that, to our knowledge, no article has dealt with yearly surveys on antimicrobial susceptibility of bacterial isolates from defined clinical specimens with the same period of each year at the same series of institutions.

Anti-Bacterial Agents↗
PubMed1985

[Comparative studies of antimicrobial agents against causative organisms isolated from urinary tract infections (1983). II. Background of patients].

We have been collected the causative isolates from patients with urinary tract infections (UTI) from the 8 institutions in Japan during 1980-1983. All strains isolated from UTI and recognized as etiologically responsible in each institution were sent to Bacteriology Laboratory of Juntendo University Hospital, Tokyo and the species of these strains were reidentified. We classified the UTI into 3 categories, simple, complicated without catheter and complicated with catheter. Of all strains isolated as etiologically responsible from cases of simple UTI, 65.3% were E. coli and 9.6%, Klebsiella spp. in 1983: these species accounted for about 75% of all isolates. Most frequently isolated from patients without catheter were E. coli 27.6% in 1983, followed in order by Pseudomonas spp. (20.9%), Gram-positive bacteria (16.7%), Serratia spp. (8.8%), Klebsiella spp. (8.0%) and Proteus spp. (7.1%). In complicated UTI with catheter, Pseudomonas spp. were most frequently isolated (25.6%), followed in order by Gram-positive bacteria (22.9%), Serratia spp. (15.0%), Proteus spp. (12.4%), Enterobacter spp. (6.0%) and Klebsiella spp. (6.0%). A remarkable difference, that is, Gram-positive bacteria, especially S. aureus, showed conspicuous increase of isolation between 1982 and 1983. Furthermore, we discussed the detailed analysis of patient background to clinical bacterial isolates from UTI in respect of patient distribution by sex, types of infections, distributions by age in male and female, interrelations between the species of bacterial isolates and types of infection and antimicrobial chemotherapy.

Administration, Oral↗