Biomedical subjects

H Tsuchiyama

Publications and source records attributed to H Tsuchiyama.

At least 37 records · Page 2Linked to original sources

PubMed1988

Eosinophilic globules in pheochromocytoma of the adrenal medulla. A histochemical, immunohistochemical and ultrastructural study.

Histochemical, immunohistochemical, and ultrastructural characteristics of eosinophilic globules in pheochromocytoma of the adrenal medulla are described. The globules were observed in 7 (63.5%) out of 11 cases of pheochromocytoma of the adrenal medulla. These globules were eosinophilic, PAS-positive with and without diastase predigestion, phosphotungstic acid hematoxylin (PTAH) positive, and autofluorescent under ultraviolet illumination. Although almost all globules were not stained with neuron specific enolase (NSE) using the immunoperoxidase method, a few globules were stained positive. The lectins of Triticum vulgaris (WGA) and Ricinus communis (RCA-120) were weakly bound to most of the eosinophilic globules. Immunohistochemical reactions for alpha-fetoprotein (AFP), alpha-l-antichymotrypsin (ACT), and human chorionic gonadotropin (HCG) revealed negative reaction in these globules. An ultrastructural study revealed no relationship between these globules and chromaffin secretory granules. These findings suggested that the eosinophilic globules in pheochromocytoma of the adrenal medulla were not related to the chromaffin secretory granules and might be some kind of complex protein.

Adrenal Gland Neoplasms↗

PubMed1988

Analysis of ras gene expression in stomach cancer by anti-ras p21 monoclonal antibodies.

Using anti-ras p21 monoclonal antibodies, RASK-3, which reacts with all of Ki-, N-, and Ha-ras p21, we examined by immunohistochemistry the expression of p21 in human gastric cancer (80 cases) and benign gastric lesions (32 cases). Ten percent formalin fixed tissues were studied. Ras p21 was positive in 51 cases (64%) out of 80 cases and partially positive in 12 cases (15%) at the cancerous areas. Ras p21 was partially positive in 7 cases (9%) at the noncancer areas of the same slides. Intestinal metaplasia and normal parietal cells were also often positive. In the study of 32 cases of benign stomach lesions, 2 out of 3 cases of atypical hyperplasia (ATP) and 3 out of 11 cases of stomach ulcer with regenerating epithelials were positive. Ras p21 was more dominantly expressed in the well-differentiated type of stomach cancer than the poorly differentiated type. Expression of ras p21, however, was not correlated either with the grades of cancer invasion or with the types of cancer infiltration.

Antibodies, Monoclonal↗

PubMed1987

Coexistent carcinoma in congenital dilatation of the bile duct and anomalous arrangement of the pancreatico-bile duct. Carcinogenesis of coexistent gall bladder carcinoma.

From 1972 to 1985, 40 cases of congenital dilatation of the bile duct (CDBD) were experienced in the department of the authors. Those consisted of 19 cases of anomalous arrangement of the pancreatico-bile duct (P-B anomaly), five of bile duct carcinoma, and two of gall bladder carcinoma. In addition, four patients who showed no dilatation of the bile duct in spite of the presence of a P-B anomaly were experienced, and coexistent gall bladder carcinoma was present in three of four cases. The incidence of bile duct carcinoma associated with CDBD is very high. However, coexistent gall bladder carcinoma in CDBD is a new topic, and coexistent intrahepatic bile duct carcinoma in CDBD is extremely rare. This report presents interesting and rare cases of coexistent carcinomas in these anomalies and investigates their carcinogenesis, particularly that of gall bladder carcinoma.

Adenocarcinoma, Papillary↗

PubMed1987

Production of collagen fibrils in adrenocortical adenoma with Cushing's syndrome.

The production of collagen fibrils, especially collagen type III, was studied with regard to eight adrenocortical adenomas with Cushing's syndrome. Anti-type III procollagen amino-terminal peptide serum (procollagen III) was employed for the PAP method. In areas of clear-type cells, the parenchymal cells were negative for procollagen III, but the stroma exhibited an intense reactivity and were arranged in an alveolar fashion. On the other hand, it was obvious that not only interstitial cells or spindle-shaped cells but also the parenchymal cells synthesized procollagen III in areas of compact-type cells, and the stroma showed a strongly positive reaction to it.

Adenoma↗

PubMed1987

Fetal ascites. A report of 3 autopsy cases.

Three rare autopsy cases of fetal ascites were presented and the etiology of each case was described. Case 1 was a male neonate, delivered by cesarean section at 32 weeks' gestation, and died of respiratory failure. The abdomen was remarkably distended with 1020 ml of ascites. The etiology of Case 1 remained unknown even after macroscopic and microscopic examinations. We considered this as "idiopathic" fetal ascites. Case 2 was a female neonate, delivered at 31 weeks' gestation, with marked abdominal distension and cyanosis. Autopsy revealed 435 ml of ascites, and she was considered to have had "polysplenia syndrome" with cardiovascular malformations. Intrauterine heart failure due to cardiac anomalies was thought to be the cause of this ascites. In case 3 embryotomy was carried out under the diagnosis of fetal ascites by ultrasound examination at 22 weeks' gestation. An urachal cyst connected to the dilated urinary bladder and deficiency of musculature of the abdominal wall composed of loose connective tissue with calcification were observed. The abdominal wall was ruptured and 1,960 ml of ascites was measured. Polycystic kidney with renal dysplasia was also found. Case 3 showed "Prune-Berry syndrome" and fetal ascites may have arisen from these anomalies.

Abortion, Induced↗

PubMed1987

Human normal and neoplastic adrenocortical cells in tissue culture observed by scanning electron microscopy.

Human normal and neoplastic adrenocortical cells were incubated under stimulation with ACTH and observed by scanning electron microscopy. Cultured normal adrenocortical cells gathered into small clusters, each cell of which had a polarity or orientation evidenced by two different aspects. In one aspect, the cell surface rounded up and microvilli protruded vertically. Pits were found among or close to the groups of microvilli. In the other aspect, the cell surface was flattened and well-developed microvilli ran horizontally. These two aspects of the cultured cells were thought to correspond to the cell surface facing the intercellular space and that facing the perisinusoidal space, respectively. In incubated cell clusters of adrenocortical adenomas with Conn's syndrome, most cells lost this polarity or orientation and unstimulated cells existed as unit of the clusters, but all adenoma cells reacted to ACTH in the same manner. Microvilli were distributed unevenly. Filopodia were noticed in some cells. Bleb-like structures appeared frequently and some of them were about to be extricated from the cell surface as in normal adrenocortical cells. Adrenocortical adenomas with Cushing's syndrome showed remarkable responses to ACTH. Their cell surface was unclean with the adherence of fragmented cytoplasm and bleb-like structures. Horizontally running elongated microvilli were almost indistinguishable from collagen fibrils. Moreover, collagen fibrils were entangled with microvilli.

Adenoma↗

PubMed1987

[Dubin-Johnson syndrome associated with hepatocellular carcinoma--report of an autopsy case].

An autopsy case of Dubin-Johnson syndrome (DJS), associated with hepatocellular carcinoma (HCC), the third such case in Japanese literature, is reported. A 64-year-old man, known to have had jaundice from his childhood, was admitted because of weight loss. Close examination revealed that the patient had DJS with HCC and a right lobectomy of the liver was performed. He died, however, of metastasis of HCC about 36 months later. At autopsy, the liver was found to be brownish-black in color. Microscopic findings of the liver were those common to HCC and chronic active hepatitis, and the brown pigment seen in the hepatocytes was mainly confined to the centrilobular ares. After histochemical and electronmicroscopic study, a close relation between this pigment and lysosome is suspected.

Carcinoma, Hepatocellular↗

PubMed1987

[Undifferentiated carcinoma of the ileum--a case report].

A 64-year-old man was admitted to Nagasaki Citizens' Hospital complaining of an abdominal tumor. Histological diagnosis was undifferentiated carcinoma of the terminal ileum. Multiple metastases of the bones were found after operation, and the patient died approximately 8 months after onset. In this paper, we describe the histological findings of undifferentiated carcinoma with a review of the literature.

Carcinoma↗

PubMed1987

[Malignant mesenchymoma of the mesocolon].

A rare case of malignant mesenchymoma in a 58-year-old woman is described. The tumor grew rapidly for a month and was located in the mesosigmoid. The resected tumor measured 25 X 20 X 20 cm and weighed 5,000 g. It metastasized into the great omentum within four months after operation. Histologically, this tumor was composed of undifferentiated sarcomatous elements, but in some parts revealed patterns of differentiation, including leiomyosarcoma, chondrosarcoma and osteosarcoma. In areas of osteosarcoma, the bone tissue varied from immature to mature. The histology of the recurrent tumors was similar to that of the first tumor. We considered that undifferentiated sarcomatous elements contained pluripotential cells which could differentiate multiple, mature malignant mesenchymes.

Female↗

PubMed1986

Adrenocortical adenoma with Cushing's syndrome. Scanning electron microscopic observations.

Adrenocortical adenomas with Cushing's syndrome (Cushing's adenoma) consisted of two different features, which intermingled in various proportions and were related with each other. The first feature showed long-columnar or trabecular arrangement and a vacuolar appearance which correspond to lipid droplets of clear-type cells. In the second feature, the sinusoid was well developed and meandering, and the perisinusoidal space was prominent and contained collagen fibrils, bleb-like structures, and granules. The collagen fibrils were entangled with the parenchymal cells. The sinusoid was lined by a sinusoidal wall with fenestrations or pores. Bleb-like structures and granules traversed them. Two different features were speculated to be consistent with areas of clear-type cells and compact-type cells, respectively, observed light and transmission electron microscopies.

Adenoma↗

PubMed1986

Adrenocortical adenoma with Cushing's syndrome in culture.

Two adrenocortical adenomata with Cushing's syndrome were examined employing cell culture methods. The results revealed that the clear-type cells changed into compact-type cells, and that the compact cells played a role in the production and secretion of steroid hormones. Furthermore, it is likely that they underwent fragmentation and produced collagen fibrils.

Adenoma↗

PubMed1986

A high-grade malignancy bronchial mucoepidermoid carcinoma with features of giant cell carcinoma.

A high-grade mucoepidermoid carcinoma associated with giant cell carcinoma of the bronchus was found in a 41-year-old man. Light and electron microscopic examinations showed glandular and squamous elements. The histology of the giant cell carcinoma was similar to those of previously reported giant cell carcinomas. It was assumed that the giant cell carcinoma in the present case is the result of transformation of mucoepidermoid carcinoma originating in the bronchial gland.

Adult↗

PubMed1986

Adrenocortical adenoma with primary aldosteronism in culture.

Four adrenocortical adenomata with primary aldosteronism (Conn's adenomata) were examined by light and electron microscopy employing cell culture methods. Unstimulated cells existed as a unit of the clusters, but Conn's adenoma cells reacted to ACTH. The cultured cells produced mostly cortisol under ACTH-stimulation as time passed. Lipid droplets in their cytoplasm decreased in number and the smooth endoplasmic reticulum (sER) was well-developed with dilated, anastomosing tubule. Moreover, mitochondria sometimes had tubulovesicular cristae. It is assumed that Conn's adenoma cells are hybrid type cells, which are intrinsically capable of producing cortisol as well as aldosterone.

Adenoma↗

PubMed1986

Structural change of adrenocortical adenoma in Cushing's syndrome.

Eight adrenocortical adenomata from patients with Cushing's syndrome were examined by light and electron microscopy. Light microscopically, the reticulin fibers were markedly prominent in areas of the compact-type cells and ran tangentially to the adenoma cells or parenchymal cells. Electron microscopically, adrenocortical adenoma was composed of parenchymal cells, interstitial cells, and endothelial cells. In areas of the clear-type cells, the parenchymal cells were arranged in long columns and covered by the sinusoidal wall. In areas of the compact-type cells, the sinusoid was well-developed and meandering. The dilated intercellular space was conspicuous and filled with filamentous substance and collagen fibrils. The parenchymal cells appeared to produce the filamentous substance and collagen fibrils, and underwent fragmentation. Interstitial cells and fragmented cytoplasm of the parenchymal cells were found in the intercellular and perisinusoidal space. It was likely that the fragmented cytoplasm flowed into the sinusoid. Experimental study using cell culture methods revealed these phenomena, especially a production of collagen fibrils.

Adenoma↗

PubMed1986

[Adenocarcinoma arising in congenital dilatation of the intrahepatic bile duct].

A multilocular lesion was found in the left lobe of the liver in a 70-year-old woman who had complained of jaundice, fever and tumor of epigastrium. There was a continuous and branching cystic lesion in the resected left lobe of the liver diffusely, and communication with the right hepatic duct and the extrahepatic duct was recognized. These cystic spaces were filled with bile and mucus. The inside of the wall was covered with columnar epithelium showing papillay proliferation with marked mucin production. Furthermore, adenocarcinoma was demonstrated in multifoci surrounded by hyperplastic and dysplastic epithelium. This case was diagnosed as congenital dilatation of the intrahepatic bile duct accompanying adenocarcinoma.

Adenocarcinoma↗

PubMed1986

[Squamous cell carcinoma of the renal pelvis associated with giant hydronephrosis--report of an autopsy case].

An autopsy case of squamous cell carcinoma of the renal pelvis associated with giant hydronephrosis, which is the fourth case in the Japanese literature, is reported. A 37-year-old man, who had been found to have a left abdominal mass was admitted because of dyspnea and lumbar pain. At autopsy the left kidney contained 2,550 ml of turbid yellowish fluid in the pelvis, with a tumor protruding from the cystic wall. Histologically, the tumor was mostly composed of squamous cell carcinoma, which showed keratinization and pearl formation, with focal transitional cell carcinoma and squamous metaplasia. The ureteropelvic junction was slightly obstructed by muscular hypertrophy and fibrosis.

Adult↗

PubMed1985

Non-functioning adrenocortical adenoma in culture. Quantitative and morphological observations.

This report describes the morphological responses of unstimulated and stimulated non-functioning adrenocortical adenoma in culture. The removed adrenocortical adenoma was composed mainly of clear-type cells and partially had a small area of cholesterol granuloma. These adenoma cells had many lipid droplets and round to long rod-shaped mitochondria with tubular or tubulo-lamellar cristae which were similar to those in Cushing's adenoma. The non-functioning adrenocortical adenoma cells which were incubated in vitro under ACTH (10 mIU/ml) and angiotensin II (10(-6) M/ml) stimulation, were examined by phase contrast microscopy, transmission and scanning electron microscopy, and the content of cortisol and aldosterone in the culture medium was measured by radioimmunoassay. As a result of exposure of ACTH, the cultured cells revealed the retraction response and production of cortisol and aldosterone. After administration of ACTH for many days, the cultured cells showed characteristic changes in sER and mitochondria. The sER were markedly developed and packed tightly into a network of dilated tubules. Mitochondria were larger and more numerous than in the unstimulated cells. The mitochondria appeared to be entwined by the tubules of the sER. Lipid droplets decreased in number.

Adenoma↗

PubMed1985

Subcutaneous sacrococcygeal myxopapillary ependymoma. A case report and review of the literatures.

A case of myxopapillary ependymoma originating in the soft tissue is described. The tumor was located subcutaneously over the coccyx of an 11-year-old girl but was connected neither to the filum terminale nor cauda equina. Clinically, the tumor was locally resected with a diagnosis of pilonidal cyst. Histological and electron microscopic findings were identical to myxopapillary ependymoma. The tumor cells showed a positive reaction by immunoperoxidase method (PAP method) of glial fibrillary acidic protein (GFAP).

Child↗