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Biomedical subjects

H Terada

Publications and source records attributed to H Terada.

At least 343 records · Page 19Linked to original sources
PubMed1982

Transport of benzenesulfonic acid derivatives through the rat erythrocyte membrane.

Transport of benzenesulfonic acid derivatives through the rat erythrocyte membrane was studied. The transport properties, such as pH-dependence and effects of reagents reacting with amino-groups, were similar to those to anions like Cl- through the human erythrocyte membrane. The rate of transport of anions through rat erythrocyte membranes is higher than through those of other mammals, such as guinea pig and bovine erythrocyte membranes. This relatively high rate of transport makes the rat erythrocyte membrane suitable for use in comparative studies on the transport of slowly penetrating substances, such as organic anions. The transport velocities of benezenesulfonic acid derivatives were compared with their physico-chemical properties. It was shown that the hydrophobicity has no effect on the transport, but the electronic property has a significant effect: the transport rate is mainly dependent on the e- donor capacities. This feature is the inverse to the well-known inhibitory effect of these derivatives on other anion transport: the inhibition is mainly dependent on the e- acceptor capacities. It is suggested that the transport is regulated by the binding capacity of anions to the transport site.

Animals↗
PubMed1982

Variant Ph1 translocations in CML and their incidence, including two cases with sequential lymphoid and myeloid crises.

A serial cytogenetic study of 110 cases of chronic myelogenous leukemia (CML) has been performed with G- and/or Q-banding techniques with the following results. (1) Seven out of the 110 cases were karyotypically normal. (2) A variant Ph1 translocation was observed in three cases. In one case, the leukemic cells contained two reciprocal translocations, i.e., a t(3;9) (q21;q34) and a t(17;22)(q21;q11); therefore, a Ph1 chromosome was masked by a translocation of the deleted material from the 17q onto the band q11 of the long arm of a chromosome No. 22. In the second case, a variant Ph1 translocation involved chromosomes No. 9, 20, and 22, resulting in a karyotype interpreted as 46,XX,t(9q+;20q+;22q-); in this rearrangement, one of the segments, i.e., 9q31 or 9q33, seemed to be interstitially deleted and inserted into the interstitial region (q11) of a chromosome No. 20 and the 22q11 leads to qter was translocated onto the 9q. This is the first case in which chromosome No. 20 was involved in a variant Ph1 translocation. In the third case, the karyotype of leukemic cells was interpreted as 46,XX,t(5;9;22)(q13;q34;q11). (3) The frequency of Ph1-negative CML and that of Ph1-positive CML with various types of Ph1 translocation from 15 studies reported as series of 25 or more cases, including the present study, have been tabulated. The incidence of a variant Ph1 translocation was 4.1% (42/1027 cases of Ph1-positive CML); of the 42, 13 were of a simple type and 29 of a complex type. (4) In one case of the present study, a masked Ph1 by a translocation of material onto the short arm of the 22q- was observed in the blastic crisis but not in the chronic phase. From the present study and a review of the published cases, it appears that the incidence of such a "masked" Ph1, which cannot be detected by conventional Giemsa staining, is less than 0.6% in CML cases. (5) The first and the second cases with a variant Ph1 translocation mentioned above developed a myeloid blastic crisis after the induction of remission of a lymphoid blastic crisis. For the present, it is unclear whether the occurrence of such blast cells in the two cases and the cytogenetic findings are coincidental. However, the evidence supports the notion of "lymphoid-myeloid" multipotentiality of certain leukemic cells.

Adult↗
PubMed1982

Synthesis and structure--activity relationship of nonyl 3-acyldithiocarbazates and related compounds for uncoupling activities.

Various nonyl 3-substituted-dithiocarbazates, methyl and dimethyl derivatives of nonyl 3-benzoyldithiocarbazate, nonyl 2-substituted-dithiocarbamates, and benzaldehyde nonyldithiocarbohydrazone were synthesized and their uncoupling activities of oxidative phosphorylation in mitochondria were examined. The results indicate that the presence of the thiocarbamoyl structure with the potential SH group is a requisite for uncoupling activity. The presence of a C=O group and a hydrophobic aromatic ring significantly increases the uncoupling activity.

Animals↗
PubMed1982

Cytogenetic and hematologic findings in acute myelogenous leukemia, M2 according to the FAB classification.

Chromosome banding studies on leukemic cells from 12 patients with acute myelogenous leukemia (AML), M2 according to the FAB classification, were performed. In 8 cases, chromosome abnormalities were observed; among these 8, 6 were found to exhibit a common chromosome abnormality, i.e., t(8;21)(q22;q22). The present findings strongly support the view that AML with t(8;21) (q22;q22) represents a definite subgroup within the general category of AML; this translocation has not been observed in more than 100 cases of leukemias other than AML M2. When the chromosome findings were collated with the hematologic and clinical data, it was found that the cases with t(8;21)(q22;q22) were not distinguishable from those with karyotypes other than the translocation in M2, in terms of hematologic and clinical findings.

Adult↗
PubMed1981

Effect of dodecyl sulfate on the spectral properties of phenylalanyl residues in serum albumin detected by second derivative spectrophotometry.

The effect of sodium dodecyl sulfate (SDS) on the spectral properties of phenylalanine residues in bovine serum albumin was studied at neutral pH by second derivative spectrophotometry. It was found that phenylalanine residues in the interior of bovine serum albumin became almost completely exposed on the surface of the protein on formation of a so-called AD12 complex. This conformational change began to be significant when 4 mol SDS bound to bovine serum albumin. At higher concentrations of SDS, when so-called ADn and AD2n complexes were formed, phenylalanine residues were transferred to the hydrophobic region again. This might be due to the involvement of phenylalanine residues in micelle-like clusters. Change in the conformation of bovine serum albumin involving tryptophan residues was also measured. These studies demonstrate the value of second derivative spectrophotometry in studies on conformational change of proteins.

Animals↗
PubMed1981

Specific requirement for inorganic phosphate for induction of bilayer membrane conductance by the cationic uncoupler carbocyanine dye.

The trinucleous divalent cationic cyanine dye triS-C4(5) was shown to be an uncoupler of oxidative phosphorylation in mitochondria only in reaction medium containing inorganic phosphate (Pi). This dye also induced marked increase in the electrical conductance of a phospholipid bilayer membrane in bathing solution containing Pi, but not in solution containing Tris-HCl buffer without Pi. Time-dependent fluctuation of the electrical current across the bilayer membrane was observed in the presence of triS-C4(5) only in bathing solution containing Pi. This fluctuation could be due to perturbation of the bilayer membrane structure induced by the cooperative action of the cyanine dye and Pi, and this perturbation should be directly related to their effects in increasing membrane conductance and also causing uncoupling in mitochondria.

Animals↗
PubMed1981

A 14q+ chromosome in a malignant lymphoma in a patient with Down's syndrome.

A 17-year-old Japanese boy with Down's syndrome developed leukemic lymphosarcoma; histology of a lymph node biopsy revealed a malignant lymphoma, of the poorly differentiated lymphocytic (ML-PDL) or possibly lymphoblastic type (ML-LB). The Giemsa-banding technique for chromosome analysis revealed the karyotype of the lymphoma cells to be 47, XY, + 21, 14q+. A chromosome study of PHA-stimulated lymphocytes showed a 21-trisomic pattern, i.e., 47, XY, + 21. The 14q+ marker was a product of a translocation in which the long arm of chromosome No. 8 (probable break at band q11) was translocated to the long arm of a No. 14 at band q32, which is a region usually affected in various types of lymphomas. Two normal No.8 chromosomes were present. Thus, the lymphoma cells were partially trisomic for chromosome No. 8.

Adolescent↗