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Biomedical subjects

H Takayasu

Publications and source records attributed to H Takayasu.

At least 55 records · Page 3Linked to original sources
PubMed1989

Cloning and characterization of mammalian homologs of the Drosophila dunce+ gene.

A probe representing the Drosophila dunce+ (dnc+) gene, the structural gene for a cAMP phosphodiesterase (PDEase), detects homologous sequences in many different organisms, including mouse, rat, and human. Genomic and cDNA clones representing a homolog of the Drosophila dnc+ gene were isolated from rat libraries and characterized. This gene has been named ratdnc-1. One cDNA clone defines a large open reading frame of approximately 1.8 kilobases (kb), predicting a protein sequence of 610 amino acids with significant homology to a conserved domain of approximately 275 residues found in most other PDEases. The amino acid identity value to the Drosophila cAMP PDEase within this domain is a striking 75%. Other cDNA clones show blocks of sequence divergence from this cDNA clone close to the predicted N terminus, indicating the potential existence of a family of related enzymes encoded by alternatively spliced messenger RNAs from ratdnc-1. Genomic blotting experiments suggest the existence of at least one other rat gene with homology to ratdnc-1. RNAs homologous to ratdnc-1 are heterogeneous in size between tissues, with heart containing a major transcript of 4.4 kb and brain one of 4.0 kb. The potential identity of the product of the ratdnc-1 gene with known PDEases is discussed.

3',5'-Cyclic-AMP Phosphodiesterases↗
PubMed1988

Flexible ureterorenoscopy.

The clinical applications of flexible ureterorenoscopes have fallen behind those of their rigid counterparts. Flexible endoscopes that possess a working channel and can also be employed for treatment have at last been developed. This instrument is particularly effective in the treatment of stones in the upper urinary tract, as it can easily follow mobile upper ureteral stones and calculi in the middle or lower renal calices, which are not as accessible with a rigid endoscope. It is important to remember not to exert too much force when inserting the instrument into the ureter, renal pelvis, or renal calices, and, if one remains calm, should the visual field become temporarily obscured, there should be little danger of damaging these structures. This method will probably be increasingly employed, not only for the treatment of stones in the upper urinary tract, but also for the removal of foreign bodies and the diagnosis and treatment of small pathologic conditions or lesions such as carcinoma in situ in which the roentgenologic presentation is unclear. For this expansion of instrument use to be ensured, it is necessary to improve the resolution of the fiberoptic endoscope image and increase the instrument's angulation capabilities, increase the range of accessories that can be employed with it, and also increase familiarity with the instruments on the part of urologists.

Endoscopes↗
PubMed1988

A case of spontaneous esophageal rupture treated with conservative therapy.

A 51 year-old male was admitted because of abrupt hematemesis and loss of consciousness. Emergency endoscopy depicted a giant esophageal ulcer on the left side extending from the middle intrathoracic region to the lower intrathoracic region. Esophagography, performed on the second day of admission, revealed a huge cavity (10 cm x 7 cm) filled with contrast medium resulting from a rupture in the ulceration. His state of shock improved with conservative therapy and no severe mediastinitis was noted. The drainage from the cavity into the lumen of the esophagus was considered to be acceptable from esophagography and an endoscopic examination. Secondary to his stable condition and continuous drainage, this patient was treated conservatively throughout his clinical course. The patient was given intravenous hyperalimentation (IVH) until the 25th hospital day and was medicated with broad spectrum-antibiotics. On the 21st hospital day the size of the esophageal cavity was markedly decreased (7 cm x 2 cm). Only small irregular mucosa was noted on the esophagogram taken 6 weeks after admission. Six months after the onset, an X-ray examination revealed complete healing of the spontaneous esophageal rupture with no recurrence.

Anti-Bacterial Agents↗