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Biomedical subjects

H Shibata

Publications and source records attributed to H Shibata.

At least 847 records · Page 47Linked to original sources
PubMed1975

The unique association of mammary stromal sarcoma with intraductal carcinoma.

The unique combination of clinically unsuspected stromal sarcoma and noninfiltrating intraductal mammary carcinoma is presented. This association has not been previously documented. The clinicopathologic features of cystosarcoma phyllodes and mammary stromal sarcoma are reviewed to provide background perspectives to illuminate the unusual aspects of this case.

Adult↗
PubMed1975

Congenital heart disease in forty years of age and over. Prevalence, relative incidence and clinical features.

The number of patients of C.H.D. over 40 years old was 40 (male 26, female 14) among 43285 (male 30320, female 12965). Its item: ASD 24, VSD 6, PDA 3, PS 3, ECD 2, Coarctation of the Aorta 1, Eisenmenger whose shunt site was unclear 1. The total prevalence rate was 0.92 per 1000 and female showed slightly higher rate than male (male 0.86 per 1000, female 1.08 per 1000). When it was compared with rheumatic valvular diseases, the ratio of both male and female was about 1/3. C.H.D. is assumed to decrease to about 1/3 or less between the period of school age and the middle age, but the prevalence rate over 60 years old was never lower than the overall prevalence investigated. C.H.D. in the middle and old aged should be kept in mind while examination.

Adult↗
PubMed1975

Isolation and purification of blasticidin S deaminase from Aspergillus terreus.

An enzyme catalyzing the deamination of the cytosine moiety of blasticidin S was extracted from a fungal strain that belongs to Aspergillus terreus. The enzyme was purified with ammonium sulfate fractionation, Sephadex G-100 column and DEAE cellulose column chromatography, followed by preparative polyacrylamide gel electrophoresis. Blasticidin S deaminase could be separated easily from co-existing cytidine deaminase by DEAE column chromatography or gel electrophoresis, and preliminary study on the substrate specificity showed that this enzyme acts on blasticidin S derivatives, such as cytomycin and acetylblasticidin S, but not on cytosine, cytidine, purine bases or their nucleosides. Blasticidin S deaminase could be induced by the addition of blasticidin S to the culture, and sulfhydryl compounds, such as mercaptoethanol, were effective in protecting the enzyme from inactivation. The homogeneity of the enzyme was examined by both sedimentation analysis and polyacrylamide gel electrophoresis. The molecular weight and isoelectric point were found to be around 30,000 and 4.35, respectively. Some other properties were also examined.

Aminohydrolases↗