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Biomedical subjects

H Seyfried

Publications and source records attributed to H Seyfried.

At least 55 records · Page 3Linked to original sources
PubMed1976

[Myocardial infarct after gastroscopy. Case report and pathogenetic considerations].

Three cases are reported of myocardial infarction and severe myocardial ischemia following gastroscopy in patients with coronary heart disease. These cases demonstrate the high risk of endoscopy in such patients. It is assumed that the main pathogenetic factors are catecholamine-mediated tachycardia and the close vicinity of the lower esophagus to the right coronary artery, with its tendency to coronary spasmus.

Aged↗
PubMed1976

[The genetics of Gilbert syndrome].

Investigation of 19 unrelated patients with Gilbert's syndrome and family studies in 21 first degree relatives of 7 of these patients are in agreement with an autosomal-dominant mode of inheritance with incomplete penetrance. The calculated penetrance in our study is 57%. HLA typing (locus A, B, C) showed a slight, insignificant increase in antigens A11 and BW35. Furthermore, this syndrome did not segregate in parallel with certain haplotypes within families.

Bilirubin↗
PubMed1975

[Liver scanning in diffuse liver disease (author's transl)].

The results of liver scans performed with 99mTc-sulphur colloid in 169 patients suffering from diffuse liver diseases and in 48 normal controls were evaluated. The patients with reactive hepatitis, acute hepatitis, chronic persistent hepatitis, fatty liver and fibrosis of the liver show only minimal deviations from the scintigraphic pattern. On the contrary, highly increased colloid uptake in the spleen is found in cases of chronic aggressive hepatitis, whilst the intrahepatic distribution of the colloid is approximately normal. In cases of liver cirrhosis, increased colloid uptake is found in the left lobe of the liver as well as in the spleen and in the bone marrow. Either normal findings or cirrhosis-like changes of the colloid distribution are observed in patients with alcoholic hepatitis.

Bone Marrow↗
PubMed1975

Serological classification of anti-I sera.

Twelve anti-I sera were examined by means of broad panel of cells: OI, BI, OhI, Oicord, Oiadult, BI-F. Titration, fixation-elution, and haemagglutination-inhibition tests, and comparison of agglutination of untreated and neuraminidase-treated erythrocytes allowed to establish the specificity of the sera. For some sera the serological heterogeneity was revealed, which consisted in the following combinations of two or more kinds of specificity: anti-I-D plus anti-I-F, anti-I-D plus anti-i, anti-I-D plus anti-I-S, anti-I-D plus anti-I-F plus anti-I-S. Methodical problems of classification of anti-I sera are discussed.

Absorption↗
PubMed1975

Reactions of erythrocyte glycoproteins and their degradation products with various anti-I sera.

Three fractions of erythrocyte glycoproteins obtained from Sepharose 4-B chromatography were tested for I activity with ten serologically differentiated anti-I sera. The most active was fraction I, eluted at the void volume and containing the lowest amount of alkali-labile oligosaccharide chains. The desialization of glycoproteins increased their activity toward anti-I-s and anti-I-D sera, and did not change or decreased the activity toward anti-I-F sera. The most abundant fraction II (major sialoglycoprotein of erythrocyte membranes) showed no or only a very weak I activity, but I-active glycopeptides were isolated from products of digestion of fraction II with trypsin. The major product of digestion, sialoglycopeptide IIT-2 showed I activity only after alkaline elimination of alkali-labile oligosaccharide chains. The results indicate that I receptors are present in hindered form on apparently I-inactive components of erythrocyte membrane.

Alkalies↗
PubMed1975

Gilbert's syndrome and HL-A. Preliminary report.

HL-A antigens were determined in 18 unrelated patients with Gilbert's syndrome and 3 families where this condition occurred in 2 generations. The data obtained do not point out an association between HL-A antigens and Gilbert's syndrome.

Gilbert Disease↗
PubMedDate not supplied

[Detection of light chains of warm-type autoantibodies in autoimmune hemolytic anemia with the aid of an autoanalyzer].

A method was evolved for detection of light chains of erythrocyte autoantibodies using a Technicon II AutoAnalyser with properly selected dilutions of bromelin and methylcellulose and anti-kappa and anti-lambda sera. It was demonstrated that the method is superior to the direct Coombs test because it made possible detection of one or both light chains in warm autoantibodies coating the erythrocytes of 20 patients with autoimmunohaemolytic anaemias in whom they could not have been detected by the manual method. It was found that in warm-type autoantibodies light chains can be detected always and that in some cases only one light chain is found.

Anemia, Hemolytic, Autoimmune↗
PubMedDate not supplied

[Antibody-dependent cytotoxic activity of peripheral blood lymphocytes in healthy subjects].

A method of determining the cytotoxic activity of peripheral blood lymphocytes against Rh-positive erythrocytes labelled with 51Cr and sensitized with anti-D antibody is described. A measure of this activity was the cytotoxic index reflecting the intensity of erythrocytolysis, and expressed as per cent of released 51Cr. The method was applied in investigations of lymphocytes of 54 blood donors. The values of the cytotoxic index ranged from 60% to 94%, the arithmetical mean was 76 +/- 10%. The obtained results are an introduction to further investigations on changes of lymphocytes K in blood diseases.

Antibody-Dependent Cell Cytotoxicity↗
PubMedDate not supplied

Auto-anti-D blocking D determinants on red cells in pregnancy.

Positive direct antiglobulin test due to IgG1 autoantibody of anti-D specificity on red cells of an apparently healthy pregnant woman was found. At the period of the highest autoantibody activity D epitope were completely blocked and even by special methods used for Rh typing in patients with AIHA the detection of D antigen was not possible. Free auto-anti-D caused benign HDN in the infant. The autoantibody production was transient and stopped after delivery. D variant included into category V on the red cells of the patient under study and her two siblings was recognized. This finding, confirmed by in vitro study, gives the explanation of the complete blocking of D epitopes by anti-D autoantibody.

Adult↗
PubMedDate not supplied

Analysis of immune response to red blood cell antigens in multitransfused patients with different diseases.

The rate of alloimmunization to red blood cell antigens in 1502 multitransfused patients, mainly with blood disorders, was analyzed in a retrospective study. The overall incidence of alloantibodies was 5.7%. Three groups of patients were identified with different potential for antibody production. The lowest probability (1.8%) of alloimmunization was found in the group of patients with lymphoproliferative syndromes, acute myeloid leukaemia and burn disease. The highest probability (33.4%) of immune response to red blood cell antigens was found in patients with AIHA, liver cirrhosis and myelodysplastic syndrome. In the group of patients with chronic myeloid leukaemia, pancytopenias, anaemias of various origin and aplastic anaemia the probability of alloimmunization ranged from 5.7% to 13.6%. A possible role of genetic-factors and immune competence status in post-transfusion alloimmunization is briefly discussed.

Adolescent↗