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Biomedical subjects

H Sakaguchi

Publications and source records attributed to H Sakaguchi.

At least 343 records · Page 19Linked to original sources
PubMed1986

Idiopathic membranous glomerulonephritis: aspects of geographical differences.

Clinicopathological studies of 89 cases of idiopathic membranous glomerulonephritis was carried out to investigate the clinical and histopathological characteristics of the disease. The results were compared with those of previous published reports to observe whether any difference between Japanese and caucasian patients exists. There were no obvious differences as to main clinical features, although an apparently better prognosis in our data was observed during long term follow up. In renal biopsy findings, especially glomerular capillary wall injuries, the prevalence of advanced electron microscopic stages (stages III and IV) was higher in the Japanese cases. A higher rate of clinical remission was observed in the treated group. It was concluded that Japanese cases had an obviously better prognosis despite having more advanced histological findings than the caucasian patients.

Adolescent↗
PubMed1986

[Clinical study of testicular tumors (59 cases in Sakai Municipal Hospital)].

Fifty-nine cases of testicular cancers were admitted to our hospital between 1960 and 1984. Thirty cases were seminoma (patients aged 12-64 years) and 29 cases were non-seminoma (6 patients were 1-7 years old and 23 were 17-44 years old). One of each of the seminoma and non-seminoma cases was not included in our analysis due to death before sufficient treatment. Of the remaining 29 cases of seminoma, 24 were in stage I, 4 in stage II, and 1 was in stage III. Of the remaining 22 adult cases of non-seminoma, 15 were in stage I, 2 in stage II, and 5 in stage III. Most of the cases with seminoma were treated with radiation after orchiectomy. Two of the cases of advanced seminoma and all of the cases of non-seminoma without mature teratoma were treated with retroperitoneal lymphadenectomy and/or chemotherapy after orchiectomy. Of the seminoma cases, only one patient (stage II cancer) died. On the other hand, only one of the non-seminoma patients in stages II and III remained alive. Since 1979, 5 patients with stage III or bulky stage II disease have been treated with the PVB protocol and 4 were evaluable. Only one patient achieved complete remission (CR) but he suffered a relapse within a short period (4 months). Three patients were partial responders and two of them achieved CR following resection of residual disease and additional VAB-6 induction. Currently, none of the patients who were treated with the initial PVB protocol alone remain in CR.

Adolescent↗
PubMed1985

The influence of antecedent renal disease on pregnancy.

The influence of antecedent renal disease on pregnancy was studied retrospectively in 72 women with various renal diseases that had been proved by biopsy. Among 105 pregnancies studied, normal deliveries were observed in 74 (71%), abnormal deliveries with live infants in 14 (13%), fetal or neonatal deaths in 11 (10%), and spontaneous abortions in six (6%). The incidence of normal delivery, as well as that of live births, was the highest in the cases of membranous glomerulonephritis, but there was no obvious difference in the incidence among IgA nephropathy and non-IgA proliferative glomerulonephritis. Cases in which there were tubulointerstitial changes of the cortical area or arteriosclerosis in biopsy specimens and cases that included hypertension (greater than 140/90 mm Hg) or decreased renal function (glomerular filtration rate, less than 70 ml/min) were clearly associated with an unfavorable outcome in delivery. It was concluded that assessment of the advisability of pregnancy in nephritic women should be made on the basis of a combination of the clinical and histologic parameters.

Abortion, Spontaneous↗
PubMed1985

Serum alpha-fetoprotein subfractions in patients with primary hepatoma or hepatic metastasis of gastric cancer.

Alpha-fetoprotein (AFP) subfractions were studied in 38 sera including 34 patients with primary hepatoma and 4 from patients with hepatic metastasis of gastric cancer. Fractionation of AFP was carried out by concanavalin A (Con A) or lentil lectin (LCH) crossed-line affinity immunoelectrophoresis. With use of Con A, fetal-liver-originated subfraction (peak a) was commonly found in both primary hepatoma and metastatic liver cancer, while yolk-sac-originated subfraction (peak b) was detected in 7 of 34 (20.6%) primary hepatomas and 4 of 4 (100%) metastatic liver cancers. With use of LCH, fetal-liver-originated subfractions (peaks A and/or C) were commonly found in both primary hepatoma and hepatic metastasis of gastric cancer, while yolk-sac-originated subfraction (peak B) was found only in metastatic liver cancer. These findings suggest that glycosylation of AFP in primary hepatoma differs from that in hepatic metastasis of gastric cancer. It is also suggested that AFP synthesized in hepatic cancers and fetal liver are differently glycosylated and AFP synthesis of hepatic malignancies are not always retrogenetically expressed, as in case of the fetal liver. Clinically, different patterns of AFP subfraction identified by Con A or LCH crossed-line affinity immunoelectrophoresis facilitate a differential diagnosis of primary hepatoma and hepatic metastasis of gastric cancer, in cases of elevated serum AFP levels. In the current study, attention was also given to the retrogenetic expression of AFP synthesis in hepatic metastasis of gastric cancer.

Carcinoma, Hepatocellular↗
PubMed1985

[Serum AFP subfractions in patients with hepatic cancer or germ cell tumor of the gonads].

Using a modified method of Con A, LCH or PHA-E affinity crossed-line immunoelectrophoresis, we studied AFP subfractions in 78 sera including 58 from patients with primary hepatoma, 11 from patients with hepatic metastasis of gastric cancer and 9 from patients with germ cell tumors of the gonads (yolk sac tumor, immature solid teratoma or mature solid teratoma). It was found that AFP in primary hepatoma, metastatic hepatoma or germ cell tumors of the gonads were differently glycosylated, and different patterns of AFP subfractions identified by Con A, LCH or PHA-E affinity crossed-line immunoelectrophoresis facilitated a differential diagnosis of such AFP related malignancies.

Carcinoma, Hepatocellular↗
PubMed1985

Alpha-fetoprotein subfractions in amniotic fluid identified by a modification of the method of concanavalin A, lentil lectin or phytohemagglutinin-E affinity crossed-line immunoelectrophoresis.

Using a modified method of concanavalin A (Con A), lentil lectin (LCH) or phytohemagglutinin-E (PHA-E) affinity crossed-line immunoelectrophoresis, alpha-fetoprotein (AFP) subfractions were studied in 33 samples of human amniotic fluid obtained between 41 and 287 days of gestation. Fetal tissues (yolk sac, liver, stomach and small intestine) obtained from a fetus of 68 days' gestation were incubated for 24 hours and AFP subfractions in the culture fluid examined. AFP in control amniotic fluids yielded two subfractions (types a and b) with Con A, three subfractions (types A, B and C) with LCH, and four subfractions (types W, X, Y and Z) with PHA-E. Serial changes of AFP subfractions in the amniotic fluid, as well as in the incubation study, indicated that the yolk sac and the gastrointestinal tract were responsible for the production of the Con A non-reactive subfraction (type b), the LCH weakly-reactive subfraction (type B) and the PHA-E reactive subfraction (types W and X), at an early stage of gestation. The Con A reactive subfraction (type a), LCH reactive subfraction (type A), PHA-E weakly-reactive subfraction (type Y) and PHA-E non-reactive subfraction (type Z) were assumed to be produced mainly by yolk sac, liver or gastrointestinal tract. We also found that the LCH non-reactive subfraction (type C) was synthesized either by liver or by the gastrointestinal tract at an early stage of gestation. At term, type a of Con A, type C of LCH and types Y and Z of PHA-E were the main subfractions in amniotic fluid, assumed to be produced by the fetal liver.

Amniotic Fluid↗
PubMed1985

Serum alpha-fetoprotein subfractions in pregnant women identified by the modified method of lectin affinity crossed-line immunoelectrophoresis.

Using a modified method of lectin affinity crossed-line immunoelectrophoresis, we studied the alpha-fetoprotein (AFP) subfractions in sera from 12 pregnant Japanese women, at 22 and 42 weeks of gestation. The method involves the first dimension electrophoresis in agarose gel containing concanavalin A (Con A) or lentil lectin (LCH), the second dimension immunoelectrophoresis in agarose gel containing polyclonal antibody against AFP, reaction with peroxidase-conjugated Protein A and staining with 4-methoxy-1-naphthol. We found that type a of Con A or type C of LCH was the only subfraction present in maternal circulation at the second or third trimester. These AFP subfractions were assumed to be of fetal liver-origin. The minimum concentration which yielded an immunoprecipitation peak was approximately 100 ng/ml, being twenty times more sensitive than the conventional lectin affinity crossed-line immunoelectrophoresis.

Antibodies↗
PubMed1985

[Transverse ectopia of the right testicle and tumor of the left testicle with immature uterus and fallopian tubes: report of a case].

A case of transverse ectopia of the right testicle and the left testicular tumor with the immature uterus and the fallopian tubes is reported. The patient, a 27-year-old married man, was admitted to our hospital with the suspicion of left testicular tumor and right undescended testicle. In his left scrotum, a double hen-egg-sized tumor was palpable and the right scrotum was empty. Exploration of the left inguinal canal disclosed double testicles with hernia uteri inguinalis. The right testicle showed transverse ectopia, and the histological finding of the left testicle was choriocarcinoma. In our review of the Japanese literature, we discovered 67 reported cases of transverse testicular ectopia. 33 (49.3%) out of 67 cases had a uterus and 9 cases (13.4%) had testicular tumor.

Adult↗
PubMed1985

[A case of non-specific inflammatory granuloma of the bladder with marked eosinophilic infiltration].

A case of non-specific inflammatory granuloma of the bladder with marked eosinophilic infiltration is reported. A 54-year-old woman was admitted to our hospital on March 9, 1983, with the suspicion of bladder cancer. Her primary complaints were miction pain, frequency and gross hematuria. Cystoscopic examination revealed a hemi-sphere-shaped tumor at the dome of the bladder. Biopsy revealed no malignancy and marked eosinophilic and macrophagocytic infiltration of the submucosa. The patient was treated with antiallergic drugs and antibiotics with excellent relief of symptoms and objective remission of bladder findings on repeated cystoscopy and computed tomography.

Eosinophilic Granuloma↗