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Biomedical subjects

H Rudolph

Publications and source records attributed to H Rudolph.

At least 37 records · Page 2Linked to original sources
PubMed1990

[Skin lesions following the use of a tourniquet].

In a questionnaire study including 44 AO-clinics in German speaking countries, we investigated frequencies and possible causes of skin lesions under the cuff occurring after surgery in a bloodless field. Though these lesions are hardly dealt with in the literature available, the number of legal claims made by patients for these lesions has significantly increased in recent years. What has to be clearly differentiated are the known changes deeply extending into the soft tissues we see in the tourniquet syndrome. The precise pathogenetic mechanisms of these lesions are unknown. The combination of fluid collecting under the cuff and pressure applied to the skin by the cuff seems to be a likely cause. The most important precautions for avoiding these skin lesions consist in correct application of the cuff and in sealing the edge of the cuff the way that at least distally it will be safely fluid-tight. Correspondingly, fluid must under no circumstances (disinfectants) be allowed to collect or pool between the operating table and resting parts of the body (sacral region!). Based on present knowledge it is not possible to safely avoid these typical skin lesions. Strict adherence to the precautions listed above and explained in more detail in the text should therefore lead to the rejection of legal claims brought in such instances.

Germany↗
PubMed1989

[Integration of malignant tumors of the head and neck region into the strategy of cancer control in the GDR].

Mainly caused by an increase of carcinomas of the mouth and of the pharynx, the incidence of malignant tumours of the head and neck increased continuously, the incidence of these tumours has doubled in males. During the last twenty years, the age distribution clearly points the younger group of patients. In addition, the percentage of advanced tumours stages III and IV are increased. A more unfavourable prognosis with an increased mortality results despite of improved therapeutic techniques. The ways how to influence this development are discussed to reach the same positive situation as in total cancer treatment in the GDR.

Adult↗
PubMed1987

PHO5 upstream sequences confer phosphate control on the constitutive PHO3 gene.

To identify the sequences involved in the regulation of the yeast acid phosphatase gene (PHO5) we constructed a series of hybrid promoters. Increasing lengths of 5'-flanking sequences of the PHO5 gene were placed in front of the TATA-box of constitutively expressed acid phosphatase gene (PHO3). The PHO5/PHO3 promoter constructions were used to replace the entire PHO5, PHO3 gene cluster on chromosome II. Depending on the length of PHO5 5'-flanking sequences present the PHO3 gene driven by the hybrid promoter could now be derepressed in response to inorganic phosphate (low Pi) exactly as the PHO5 wild type gene. A critical regulatory element was located between position -402 to -351 (upstream from ATG) and sequences further downstream (from -351 to -300) could increase transcriptional activation. The transcription levels of PHO3 were determined by northern blot analysis, under repressed (high Pi) and derepressed (low Pi) conditions which was paralleled by an increase in extra-cellular acid phosphatase activity. Fully regulated promoter hybrids showed a 40-fold induction of mRNA levels, comparable to wild type PHO5 promoter. Sl-nuclease protection experiments revealed that the PHO5 5'-flanking sequences, placed in front of PHO3, did not change the PHO3 transcription initiation site/s.

Acid Phosphatase↗
PubMed1987

[Femoral shaft fracture. Treatment and results of 209 fractures].

This is a report of treatment, complications and results of 209 fractures of the femoral shaft: 114 compressions plates, 63 medullary nails, four fixateur externe, 28 vertical tractions after Weber. The most frequent complication after compression plate was the delayed fracture healing (5.9%) and the malrotation after medullary nails (8%). 2% of the cases developed an osteitis, no pseudoarthrosis was observed. All complications could be successfully treated by second operation. 92.8% of the long-term results could be judged as good to very good. The fixateur externe was rarely used, the vertical traction after Weber in childhood proved to be successful in spite of some complications.

Adolescent↗
PubMed1987

The yeast PHO5 promoter: phosphate-control elements and sequences mediating mRNA start-site selection.

Transcription of PHO5 is strongly regulated in response to the level of inorganic phosphate (Pi) present in the growth medium. We have identified elements required for PHO5 expression by analyzing small deletions in the PHO5 promoter on chromosome II. The results reveal three functionally different components of the PHO5 promoter: regulatory regions, a "TATA" element, and specific mRNA initiation sites. The regulatory regions contain related 19-base-pair (bp) dyad sequences acting as phosphate-controlled upstream activation sites (UASpS). These UASpS mediate the transcriptional activation of PHO5 observed in low Pi conditions. The unlinked but coordinately regulated PHO11 promoter contains a single copy of an almost identical dyad sequence, suggesting that there is a common regulatory UASp for both genes. A TATA element is absolutely required for detectable PHO5 transcription. Specific purine-pyrimidine motifs (RRYRR) (R = purine and Y = pyrimidine) serve as PHO5 mRNA initiation sites, but only if they lie 55-110 bp downstream of a functional TATA element. Such an "initiation window" is not found in higher eukaryotes and implies mechanistic differences in the transcription machineries between yeast and higher eukaryotes.

Base Sequence↗
PubMed1987

[Not Available].

Explore the source record for details and available documents.

History, Early Modern 1451-1600↗
PubMed1987

[Bore wire osteosynthesis in distal radius fracture].

Treatment results and complications of 404 percutaneous drill wire fixations in patients with dislocated radial fracture of typical situation will be reported. The most frequent complications were secondary impression of fracture, transient paraesthesia as well as perforation through the skin by drill wire. There was no case of osteitis or pseudo-arthrosis. Treatment results were judged excellent or good in 84% of cases, satisfactory in 11% and poor in 5%. For dislocated wrist fractures and for elderly patients osteosynthesis with percutaneous drill wire according to Willenegger is superior to non-operative procedures.

Adult↗
PubMed1986

Removal of positioned nucleosomes from the yeast PHO5 promoter upon PHO5 induction releases additional upstream activating DNA elements.

The chromatin fine structure in the promoter region of PHO5, the structural gene for a strongly regulated acid phosphatase in yeast, was analyzed. An upstream activating sequence 367 bp away from the start of the coding sequence that is essential for gene induction was found to reside in the center of a hypersensitive region under conditions of PHO5 repression. Under these conditions three related elements at positions -469, -245 and -185 are contained within precisely positioned nucleosomes located on both sides of the hypersensitive region. Upon PHO5 induction the chromatin structure of the promoter undergoes a defined transition, in the course of which two nucleosomes upstream and two nucleosomes downstream of the hypersensitive site are selectively removed. In this way approximately 600 bp upstream of the PHO5 coding sequence become highly accessible and all four elements are free to interact with putative regulatory proteins. These findings suggest a mechanism by which the chromatin structure participates in the functioning of a regulated promoter.

Acid Phosphatase↗
PubMed1985

One-step gene replacement in yeast by cotransformation.

A general method to replace chromosomal DNA sequences of Saccharomyces cerevisiae by any in vitro modified DNA sequence has been developed and was applied to the PHO5 locus on chromosome II. A recipient strain was constructed in which part of the chromosomal PHO5 sequence was substituted by the URA3 gene. Replacement of this pho5-URA3 substitution by pho5 mutant alleles was achieved in one step by cotransformation with a pho5 DNA fragment and the self-replicating plasmid YEp13, which contains the LEU2 gene as a selectable marker. Leu+ transformants were selected, and the replacement events at the PHO5 locus were detected by their Ura- phenotype (1-4% of the Leu+ were Ura-). In a similar way the PHO5 coding sequence was replaced by the sequence coding for human tissue-type plasminogen activator (t-PA).

Alleles↗
PubMed1985

[Effect of erythropoiesis, size of iron accumulation and oxygen saturation of tissues on iron resorption].

The volume of iron resorption is altered by numerous factors. To a wide extent, the exact regulating mechanisms have not been elucidated to the present day. The present paper deals with representing the influence of iron resorption by erythropoiesis, filling condition of iron stores and oxygen saturation of the tissue as well as with main aspects which are relevant for future research.

Absorption↗
PubMed1984

Structural analysis of the two tandemly repeated acid phosphatase genes in yeast.

We have sequenced the genetically linked genes for repressible (PHO5) and and constitutive (PHO3) acid phosphatase from S. cerevisiae. Both genes are located on a 3.91 Kb BamHI and HpaI fragment, in the order (5') PHO5, PHO3 (3'). The mRNA transcripts have been analysed by S1-nuclease mapping. They show heterogenous initiation sites. Each of the PHO5 and PHO3 genes codes for 467 amino acids as deduced from the DNA sequence. The coding regions of the two genes show homology both at the nucleotide (82%) and the amino acid (87%) level. In the coding sequences, long stretches of homologous regions are flanked by small non-homologous regions. The nucleotide homology (65%) extends to some length into the 5' and 3' non-coding flanking sequences. Further upstream sequences are unrelated. The comparison of the NH2-terminal amino acid sequence deduced from the nucleotide sequence, with that of purified repressible acid phosphatase revealed the presence of a putative signal peptide.

Acid Phosphatase↗