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Biomedical subjects

H Okabe

Publications and source records attributed to H Okabe.

At least 469 records · Page 26Linked to original sources
PubMed1986

[Cell kinetics and histological types of gastric cancer as studied by DNA-RNA cytofluorometry after acridine orange fluorescence staining].

In order to clarify the relation between histological classification and biological behavior of individual tumor cells, we quantitatively analyzed the cell kinetics of 56 advanced gastric cancers using DNA-RNA cytofluorometry after acridine orange fluorescence staining and compared the results with histological findings. The gastric cancers examined could be classified into two main groups based on the ploidy patterns constituting the cell population kinetics. In group I, tumor cells were found to be chiefly composed of diploid cells with many S-phase cells even at their advanced growth stages, and their morphological characteristics were shown to correspond to undifferentiated carcinoma (according to Nakamura & Sugano). In group II, tumor cells were composed of various classes of polyploid cells as well as of diploid cells with an increased number of S-phase cells, and the extent of polyploidization appeared to progress with the growth of the tumor. The morphological characteristics of this group tended to correspond to differentiated adenocarcinoma. It is, therefore, concluded that both the histological structures and cell morphology have close relationships with cell kinetics in the human gastric cancers.

Acridine Orange↗
PubMed1985

Progressive cone dystrophy associated with low alpha-L-fucosidase activity in serum and leukocytes.

The enzyme activities of acid phosphatase, beta-glucuronidase, N-acetyl-beta-D-glucosaminidase, and alpha-D-mannosidase were not significantly different in patients with myopia, retinal detachment, hereditary macular dystrophy, and unusual progressive cone dystrophy. alpha-L-Fucosidase activity in sera was lower in three patients with myopia and in two patients with unusual progressive cone dystrophy than in most of the others. Leukocytic alpha-L-fucosidase activity was lower in those with unusual progressive cone dystrophy. The two unrelated patients with unusual progressive cone dystrophy had slowly deteriorating visual acuity, color vision, and photopic electroretinographic responses, but ophthalmoscopically normal fundi and noncontributory family histories.

Adolescent↗
PubMed1985

Glomus tumor of the cheek: an immunohistochemical demonstration of actin and myosin.

A rare case of a buccal glomus tumor was examined by light and electron microscopy. Histologically, the tumor consisted of various vascular spaces surrounded by masses of epithelioid glomus cells, coexisting with a hemangiomatous proliferation of the vessels. Immunohistochemical stainings revealed that the tumor cells were positive for smooth-muscle actin and myosin, with various intensities of the stainings. The electron microscope invariably showed microfilaments in the tumor cells. The results suggest that the glomus tumor cells have characteristics of the smooth-muscle cell and the tumor has a hamartomatous nature.

Actins↗
PubMed1985

Smooth and skeletal muscle myosins in spindle cell tumors of soft tissue. An immunohistochemical study.

Histological localization of myosins of the smooth and skeletal muscles was investigated in comparison with that of myoglobin by immunoperoxidase technique using the antibody against each of them in surgical specimens from spindle cell tumors and tumor-like lesions of the soft tissue. Skeletal muscle myosin was demonstrated in all of the cases of rhabdomyosarcoma, whereas myoglobin was found in 75% of the examined cases. Smooth muscle myosin was widely distributed not only in the tumor cells of smooth muscle origin such as leiomyosarcoma and angioleiomyoma, but also in the tumor cells showing myofibroblastic differentiation such as malignant fibrous histiocytoma and in the epithelial components of synovial sarcoma. The results showed that skeletal muscle myosin can be regarded as an excellent marker in the diagnosis of rhabdomyosarcoma and that smooth muscle myosin is a useful marker of leiomyosarcoma and leiomyomas, and of tumors with myofibroblastic differentiation.

Adolescent↗