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Biomedical subjects

H Ohno

Publications and source records attributed to H Ohno.

At least 523 records · Page 29Linked to original sources
PubMed1986

Maternal plasma concentrations of catecholamines and cyclic nucleotides during labor and following delivery.

In plasma obtained from seven mothers before, during, and after normal labor and delivery, catecholamine and cyclic nucleotide concentrations were investigated. Dopamine concentration showed a significant elevation on admission to hospital in labor and there were marked increases in norepinephrine and epinephrine concentrations during labor at 10 cm cervical dilatation and immediately after delivery, respectively. No significant change in dopa, cAMP, or cGMP was found during the experimental period. However, since the van Beaumont quotient (J.Appl. Physiol. 34, 102-106) for cAMP did not follow the reduction in plasma volume, the concentration appeared to rise during labor. Positive correlations were observed between epinephrine on one hand, and heart rate and systolic blood pressure on the other, as well as between norepinephrine and cAMP, respectively, during labor. The diminution of epinephrine on the fourth day postpartum might reflect a reduction of emotional stress concomitant with labor. cAMP was found to be quickly cleared from the bloodstream within 2 hours after delivery. These results suggest that plasma concentrations of epinephrine and cAMP, especially epinephrine, are indices of maternal psychological and physiological stress during labor and following delivery.

Adult↗
PubMed1986

Zinc metabolism in human blood during acute exposure to cold.

Effects of acute exposure to cold (10 degrees C for 60 min) on the concentrations of zinc and related proteins in red blood cells and in plasma were studied on seven male students. Lower concentrations of total zinc and of zinc derived from carbonic anhydrase I (CA-I) in red blood cells were found during cold exposure and 15 min after cold exposure, whereas no significant changes occurred in the concentration of zinc derived from carbonic anhydrase II (CA-II) or Cu2Zn2 superoxide dismutase (SOD1). The decrease in total zinc concentration in red blood cells was mostly due to that in CA-I concentration. On the other hand, there were not any changes in total zinc, albumin-bound zinc, and alpha 2-macroglobulin-bound zinc concentrations in plasma during the experimental period. These results, together with the marked hemoconcentration during cold exposure, suggest that cold stress induces an efflux of zinc from plasma.

Acclimatization↗
PubMed1986

Establishment of a novel acute monoblastic leukemia cell line (YK-M2) having a near-triploid karyotype.

The YK-M2 cell line was established from the peripheral blood of a patient with acute monoblastic leukemia in whom an anterior mediastinal tumor preceded the peripheral blood manifestation. The established cells grew in a single cell suspension with a doubling time of 60 h and consisted of primitive monoblastic cells. The cells were 52% positive for peroxidase staining and manifested strongly positive activity of alpha-naphthyl acetate esterase, which was completely inhibited by sodium fluoride. The cells showed strong expression of Fc gamma receptors and phagocytosed sensitized ox erythrocytes. When the cells were incubated with 1 alpha,25-dihydroxy-vitamin D3, they were induced to differentiate into mature monocyte-macrophage-like cells, which reduced the nitroblue tetrazolium dye and released a small amount of the superoxide anion. Cytogenetic studies revealed that the cells had a near-triploid karyotype with a modal chromosome number of 68, and the short arm of one No. 17 chromosome was deleted [del(17)(p11)]. The YK-M2 cell line is particularly unique in that the cells retained the polyploid karyotype that may be an initial cytogenetic change in the malignant transformation of the parent leukemia cells.

Adult↗
PubMed1985

Solubilization and molecular weight estimation of atrial natriuretic factor receptor from bovine adrenal cortex.

Receptors for atrial natriuretic factor (ANF) have been solubilized with 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate from bovine adrenal cortex and characterized. The detergent extract retained specific high-affinity binding sites for 125I-ANF. Scatchard analysis of the equilibrium binding data revealed a single class of binding site with a K-d of 1.8 nM and a maximum binding capacity of 2.5 pmol/mg of protein. The size of the 125I-ANF X receptor complexes was estimated to be 140,000 daltons by gel filtration on TSK gel G3000SW. Affinity labeling followed by electrophoresis under nonreducing conditions and autoradiography also revealed a single band of a similar size (Mr = 130,000); this band, however, migrated as a Mr = 70,000 species under reducing electrophoretic conditions. These results indicate that the ANF receptor, having a Mr of 130,000 - 140,000, is composed of disulfide-linked subunits and the ANF-binding site is located on the 70-kDa component.

Adrenal Cortex↗
PubMed1985

A study of zinc distribution in erythrocytes of normal humans.

The assignment of zinc bound to carbonic anhydrase isoenzymes (CA-I and CA-II) and Cu2 Zn2 superoxide dismutase (SOD1) was investigated in the hemolysates from 21 normal male subjects. Sufficient care was taken to remove leukocytes and platelets. The following values of zinc distribution were obtained: total zinc, 113.8 +/- 22.7 (mean +/- S.E.) micrograms X 100 ml-1; CA-I-derived zinc, 866.6 +/- 26.2 micrograms X 100 ml-1; CA-II-derived zinc, 99.9 +/- 3.9 micrograms X 100 m-1; SOD1-derived zinc, 60.3 +/- 1.9 micrograms X 100 ml-1; the other zinc, 87.0 +/- 12.6 micrograms X 100 ml-1. Namely, 7.6% of the zinc in human erythrocytes is not bound to the carbonic anhydrases and Cu2Zn2 superoxide dismutase, but present in available form or attached to other enzymes.

Adult↗
PubMed1985

Secondary cystic lesions in the cerebellum in very low birth weight infants.

We studied 4 very low birth weight infants with secondary cystic lesions in the cerebellum by serial cranial ultrasound and computed tomography. These infants presented severe respiratory distress, asphyxia at birth and intraventricular hemorrhage. In the neonatal period, cranial ultrasound examination showed significant intraventricular hemorrhage and posthemorrhagic ventricular dilatation with intraparenchymal hemorrhage. However, a cerebellar cyst was not seen in any of the infants. We found evidence of cerebellar hemorrhage by ultrasound in only one patient. Follow-up ultrasound examinations and CT scans showed progressive posthemorrhagic ventriculomegaly in three of the infants and large cystic lesions in the infratentorial area from 3 to 6 months old in all 4 infants. We conclude that the cystic lesions were caused by hemorrhagic and hypoxic-ischemic insults which occurred in very low birth weight infant.

Cerebellar Diseases↗