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Biomedical subjects

H Ohmori

Publications and source records attributed to H Ohmori.

At least 397 records · Page 22Linked to original sources
PubMed1979

In vitro microbiological evaluation of TEI-1194 and TEI-2012, novel antipseudomonal semisynthetic penicillins.

TEI-1194, sodium 6-[D-(-)-alpha-(coumarin-3-carboxamide)-phenylacetamide] penicillanate and TEI-2012, sodium 6[D-(-)alpha-(8-hydroxy-coumarin-3-carboxamide)-phenylacetamide] penicillanate are new semisynthetic penicillin derivatives both possessing a broad spectrum of in vitro antibacterial activities. Minimal inhibitory concentrations of both agents were compared with carbenicillin. TEI-1194 and TEI-2012 were clearly found to have more potent activities especially against Pseudomonas aeruginosa than carbenicillin. At a concentration at 6.25 micrograms/ml, 85 approximately 90% of a total of 50 strains of clinically isolated P. aeruginosa were inhibited by TEI-1194 and TEI-2012, whereas carbenicillin had no effect. Evaluation of the antibacterial activity against a series of mutants producing different levels of beta-lactamases and test of the susceptibilities to some beta-lactamases demonstrated that TEI-1194 and TEI-2012 had low susceptibility to various cephalosporinases. However, both compounds were susceptible to penicillinase from Klebsiella pneumoniae H-2 at a rate of about 15% of penicillin-G taking its absolute rate as 100.

Bacteria↗
PubMed1979

Sequential radioimmunoassay of unconjugated and conjugated estrogen in male human plasma.

A sequential radioimmunoassay procedure for unconjugated and conjugated estrone, estradiol-17 beta and estriol in male human plasma was developed. The blank values in this assay for unconjugated estrone, estradiol-17 beta, estriol conjugated estrone, estradiol-17 beta and conjugated estriol were 0.36 +/- 1.14 pg, 3.90 +/- 2.75 pg, 2.25 +/- 2.08 pg, 0.92 +/- 1.51 pg, 5.02 +/- 2.86 pg and 3.12 +/- 2.97 pg, respectively. Mean values of unconjugated estrone, estradiol-17 beta, estriol, conjugated estrone, estradiol-17 beta and conjugated estriol in plasma from 28 normal adult males were 38.4 +/- 13.4 pg/ml, 32.6 +/- 9.90 pg/ml, 4.06 +/- 3.68 pg/ml, 34.2 +/- 13.8 pg/ml, 40.4 +/- 12.3 pg/ml and 31.8 +/- 7.41 pg/ml, respectively. Both unconjugated and conjugated estrogen levels in patients with liver cirrhosis were elevated and conjugated estrogen, especially estriol levels, in patients with renal insufficiency were markedly elevated.

Acute Kidney Injury↗
PubMed1979

Malacoplakia of probable retroperitoneal origin.

A case of extravesical malacoplakia, the first case in Japan, is described in detail. The patient was a 61-year-old woman with a right flank mass. Radiologically, the mass was thought to be of the renal origin. Surgically, however, the tumor was found attached not only to the cortical surface but extended to the retroperitoneum and psoas muscle. Pathological examination confirmed the lesion to be malacoplakia characterized by the presence of von Hansemann cells and Michaelis-Gutmann bodies.

Female↗
PubMed1978

Prostaglandin I2 as a potentiator of acute inflammation in rats.

Prostaglandin I2 potentiated the paw swelling induced by carrageenin in rats. Prostaglandin I2 (0.1 microgram) showed similar activity to PGE1 (0.01 microgram). This potentiating property disappeared in 60 minutes and was completely abolished by diphenhydramine (25 mg kg-1, i.p.). In vascular permeability tests, PGI2 itself (2.5 X 10(-10) mol, 88 ng) caused no dye leakage reaction, but PGE1 (2.5 X 10(-10) mol, 88.5 ng) caused a significant dye leakage. This effect of PGE1 was statistically significant compared with vehicle- or PGI2-treated groups (p less than 0.05). Prostaglandin I2 potentiated the increased vascular permeability induced by 5-hydroxytriptamine (2.5 X 10(-10) mol), bradykinin (5 X 10(-10) mol) and histamine (2 X 10(-10) to 2 X 10(-8) mol). The potentiation was the most evident in the case of histamine.

Acute Disease↗
PubMed1978

Detection of 5-methylcytosine in DNA sequences.

Col E1 DNA has methylated cytosine in the sequence 5'-CC*(A/T)GG-3' and methylated adenine in the sequence 5'-GA*TC-3' at the positions indicated by asterisks(*). When the Maxam-Gilbert DNA sequencing method is applied to this DNA, the methylated cytosine (5-methylcytosine) is found to be less reactive to hydrazine than are cytosine and thymine, so that a band corresponding to that base does not appear in the pyrimidine cleavage patterns. The existence of the methylated cytosine can be confirmed by analyzing the complementary strand or unmethylated DNA. In contrast, the methylated adenine (probably N6-methyladenine) cannot be distinguished from adenine with standard conditions for cleavage at adenine.

Base Sequence↗
PubMed1978

Inactivation kinetics and steady-state current noise in the anomalous rectifier of tunicate egg cell membranes.

1. Inward K current through the anomalous rectifier in the tunicate egg (Halocynthis roretzi, Drashe) was studied under voltage clamp. The transient inward current in response to a step change of membrane potential was measured. The steady-state current fluctuations were analysed using the power density spectrum (p.d.s.). 2. The inward current showed time-dependent changes, which were described by a pair of the first order kinetic parameters, n and s for activation and inactivation, respectively. The steady-state channel open probability due to the activation process (n infinity) was assumed to be 1.0 for V more negative than about--100 mV, but that of the inactivation process (s infinity) and the time constant of inactivation (taus) were membrane potential dependent in the same potential range; both decreased with increasing hyperpolarization. 3. The inward currents in Na-free choline medium did not inactivate, but were decreased in size. In Na-free Li medium, inactivation was very small; the steady-state conductance was not affected significantly. 4. After exposure to high Ca media, an increase of the conductance was observed. This effect is probably caused by an increase of intracellular Ca due to Ca ions entering through the Na channels. Mg ions slightly decreased the conductance. 5. In the hyperpolarized membrane (-160 less than or equal to V less than or equal to -80mV), steady-state current noise was recorded and analysed using p.d.s. A p.d.s. of the 1/[1 + (f/fc)2] type as well a p.d.s. of the 1/f type was observed; f, frequency, fc, cut-off frequency. 6. fc was translated into time constant tauN (= 1/2pIfC) and compared with the time constant of inactivation, taus. There was a significant correlation betwen these values with a regression coefficient of 0.82. 7. Changing from 400 mM-Li abloshied inactivation and changed the p.d.s. from the 1/[1 + (f/fc)2] into the 1/f type. These results (paragraphs 5--7)suggest that the fluctuations in the steady-state currents originatte in the inactivation gatin kinetics of the an ofthe anomalous rectifier. 8. The number of anomalous rectifier channels and the unit channel conductance were estimated from the 1/[1 + (f/fc)2] type current noise according to the formula : (see text), where I infinity = gamma Nninfinity s infinity (V--VK), gamma the unit channel conductance, N the maximum number of channels that can be opened by a hyperpolarizing pulse per egg. The unit conductance was 6 pmho in standard artificial sea water and the channel density was 0.028/micrometer2. 9. The unit channel conductance (gamma) was dependent upon external K concentration, but the number ofchannels (N) was not. 10. The increase in chord conductance evoked by higher Ca concentrations was due to the increase of the channel number. By contrast, Mg ions seem to decrease the unit channel conductance slightly.

Animals↗
PubMed1978

A correlative study on urinary cytology and FDP with cystoscopy and pathology in the urinary bladder cancers.

In view of comparatively-high incidence of papillary, well or moderately-well differentiated, transitional cell carcinomas in urology, and of frequent recurrence of carcinomas after appropriate procedures, we have undertaken the correlative study on urinary cytology with cystoscopy, pathology and the amount of fibrinogen and degradation products of fibrinogen and fibrin (FDP). Cytopathologically, 191 cases were confirmed histologically for cancers out of 442 cases studied cytologically; among these 442 classes IV and V were found in 136 cases, and 99 of 136 cases (72.8%) had cancers. Admittedly, we were unable to suspect tumors cytologically in 24.5% of the cases. As to histological gradings and cytology, 48.7% of classes I and II in well differentiated were found against 52.9% and 57.1% of classes IV and V in moderately-well and poorly differentiated carcinomas, respectively. Urinary FDP assay was quite promising; out of 19 cases with both positive cytology and FDP, 18 (94.7% had carcinomas histologically, whereas 12 of 56 cases (21.4%) with both negative cytology and FDP bore cancers. Subsequently, several proposals for improving the diagnostic accuracy were discussed. We concluded the importance of accumulating more cases by combining several diagnostic procedures, especially among well and moderately-well differentiated carcinomas.

Carcinoma, Transitional Cell↗
PubMed1977

Origin of replication of colicin E1 plasmid DNA.

Cleavage maps of colicin E1 plasmid DNA and its smaller derivative, pNT1 DNA, were constructed by using restriction endonucleases. The nucleotide sequence of a region that contains the orgin of replication was determined. The site of the nucleotide from which DNA replication is initiated was determined with 6S L-fragments, the DNA fragment first made on colicin E1 plasmid DNA. The fragments were labeled with [gamma-32P]ATP and polynucleotide 5'-hydroxyl-kinase (ATP:5'-dephosphopolynucleotide 5'-phosphotransferase, EC 2.7.1.78) at the 5'-OH groups which were uncovered by alkali treatment. The site is one of three consecutive nucleotides, dA, dA, and dC, located at a unique position. One or a few rA residues were found to be attached to some of the DNA molecules. The transition from the primer RNA to DNA occurs in a region consisting of a segment of five A residues. Both sides of this segment are rich in G and C.

Base Sequence↗