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Biomedical subjects

H Nielsen

Publications and source records attributed to H Nielsen.

At least 397 records · Page 22Linked to original sources
PubMed1981

Human monocyte function in vitro: influence of conditions during transport of blood from patient to laboratory.

Monocyte functional tests were carried out on samples of peripheral human blood which had been subjected to 4 different conditions of transport and temperature. There was no difference in cell viability or pinocytic, phagocytic and chemotactic activity of monocytes isolated from blood exposed to these different conditions. However, the yield of monocytes obtained after metrizoate/polysucrose centrifugation was very low when blood samples in transit were kept for 2 h at 0--4 degrees C. It is concluded that a period of transport up to 24 h is acceptable, provided the temperature of the blood sample is kept between 15 and 25 degrees C.

Adult↗
PubMed1981

Burkitt's lymphoma mimicking prostatitis.

We report on a 24-year-old man who had symptoms suggestive of acute pancreatitis but the diagnosis was Burkitt's lymphoma infiltrating the prostate. He was treated with cytotoxic drugs with a good initial response. Voiding improved and the tumor had disappeared completely on rectal palpation. However, the patient suffered a relapse and, despite intensive chemotherapy, he died a few months later with disseminated tumor infiltration.

Adult↗
PubMed1981

Possible role of histamine in rheumatoid arthritis. Treatment with cimetidine and mepyramine.

Basophilocytes from patients with rheumatoid arthritis (RA) responded to leukocyte nuclei from normal persons with histamine release; a similar histamine release induced by the nuclear components RNA and DNA has been demonstrated previously. A role of histamine in RA is also supported by the findings of clinical improvement during treatment with H1 and H2 antihistamines in six of 12 patients with RA in active phase, whereas four showed definite deterioration.

Adolescent↗
PubMed1981

In vitro characterization of synthesized thyroglobulin immune complexes (IC). A model for studies of IC containing an autoantigen.

Human thyroglobulin (Tg) immune complexes (TgIC) were preformed at different ratios from purified Tg, and Tg antibodies (TgAb) obtained from six patients with autoimmune thyroid diseases. TgIC were characterized by precipitation with polyethylene glycol, and sedimentation in sucrose gradient. TgIC from one patient were further characterized by concanavalin A (con A), and binding to the complement factor Clq and rheumatoid factor (RF). Tg and TgIC had almost identical binding to con A, no binding to Clq, but could be partially separated by ultracentrifugation and polyethylene glycol. The highest degree of separation was obtained by a RF-coated plastic tube radiometric assay, using 125-I-rabbit TgAb as indicator. Tg showed no binding to RF, and a dose-response curve of TgIC in serum could be established. There was a dependence of the Tg-TgAb ratio, TgIC at equilibrium and in antibody excess being detected most efficiently. The method may serve as an aid in the evaluation of the fate of Tg, TgAb and TgIC following thyroid damage (surgery, radioiodine) and may be extended as a model system in the investigation of immune complexes in connection with autoimmune disorders.

Antigen-Antibody Complex↗
PubMed1981

Personality correlates of sphenoidal EEG-foci in temporal lobe epilepsy.

Patients with unilateral temporal lateral or temporal mediobasal epileptic focus as ascertained by sphenoidal electrode EEG recordings were evaluated using the questionnaire designed by Bear & Fedio (1977). The seventeen traits defined by the items in this questionnaire were also assessed by close observers in an equivalent questionnaire. Patients with medio-basal temporal lobe focus were found generally to exhibit "epileptic" personality traits to a greater extent than patients with lateral focus, and the results indicated that they also, more than patients with lateral focus, were characterized by a schizoid paranoid outlook. The patients with left temporal lobe focus were found to be emotionally labile compared to patients with right temporal lobe focus. Patients with lateral right-sided temporal focus had obviously the most benign psychological prognosis. The main discrepancies between the results of Bear & Fedio (1977) and the present study are briefly discussed.

Adolescent↗
PubMed1981

Methodological approaches to antigen identification in soluble immune complexes; A model study.

The usefulness of different techniques for identification of immune complex-(IC) bound antigen(s) was investigated in two Ab-Ag model systems. The following approaches were used: 1) determination of IC-activity before and after removal of IC by adsorption to antibody-specific solid phase systems. 2) analysis of PEG-insoluble IC by reference line-rocket immunoelectrophoresis and 3) reprecipitation of PEG-insoluble IC in the presence of 125I-labelled antibody to suspected antigens and subsequent analysis of the distribution of the radioactivity. This latter approach, the use of a double PEG precipitation immunoradiometric assay, was found most useful and simple for identification of antigen(s) contained in soluble IC. The technique is most sensitive in demonstrating IC-bound antigen(s) when the IC are formed in slight to moderate antigen excess.

Adsorption↗
PubMed1981

Carbon dioxide tensions in manually ventilated, prone patients.

The distribution of arterial carbon dioxide tensions (PaCO2) in 288 anaesthetized, healthy patients in the prone position was investigated during non-monitored manual ventilation. Four equal groups of 72 patients were compared employing a conventional anaesthetic system with and without CO2-absorption and a modified Mapleson D rebreathing system using high and low fresh gas flows. No fundamental difference between the Mapleson D system and the circle system without CO2-absorption could be demonstrated. With the Mapleson D system a high fresh gas flow resulted in a loss of rebreathing characteristics, the scatter of PaCO2-values then approaching that of a conventional circle system with CO2-absorption. The results demonstrate that during manual ventilation a CO2-absorption results in unpredictable levels of PaCO2 with unacceptably wide ranges. On the other hand, prevention of hypocapnia could be obtained with the rebreathing systems using a low fresh gas flow. Hypercapnia was modest and similar in all groups, PaCO2 never exceeding 7.3 kPa. In terms of PaCO2-levels it seems, compared to recent literature, of no importance whether the patient is prone or supine or whether or not ventilation is monitored, performed by hand or by means of anaesthetic ventilators. Accurate levels of PaCO2 can probably only be obtained by frequent analyses of PaCO2, or monitoring of the end-tidal CO2 concentration.

Adult↗
PubMed1980

Cross-infection from contaminated anaesthetic equipment. A real hazard?

A definite relationship between the use of contaminated anaesthetic equipment and subsequent pulmonary infection remains to be established. There is however indirect and circumstantial evidence suggesting that cross-infection may occur, and further an increased susceptibility of surgical patients to pulmonary infections has been demonstrated. Decontamination should be recommended before the equipment is re-used. Pasteurisation may prove sufficient and this can be obtained employing a specially designed dish-washing machine.

Anesthesia, Inhalation↗
PubMed1980

Soluble immune complexes in cerebrospinal fluid of patients with multiple sclerosis and other neurological diseases.

The occurrence of soluble immune complexes (IC) in the cerebrospinal fluid (CSF) of 14 multiple sclerosis (MS) patients, four acute polyradiculoneuritis patients, 30 patients with other neurological diseases (OND) and 30 patients with disc prolapse (DP) was examined by a solid phase C1q-protein A binding assay (C1q-PABA) and a complement consumption test. IC-positive reactions were observed only in the C1q-PABA. The binding indices determined by the C1q-PABA differed significantly (P < 0.01) when the MS or the OND patient groups were compared to the DP group. No significant (P < 0.1) difference was observed between the indices in the MS and OND groups. Binding indices in C1q-PABA showed no correlation either to IgG concentration, total protein concentration or cell counts in CSF of MS patients. Three of the four polyradiculoneuritis patients were strongly IC-positive while the fourth patient was negative. Filtration and PEG-precipitation data indicated that a major part of the IgG-containing IC in CSF detected by C1q-PABA was of macromolecular nature.

Adolescent↗
PubMed1980

Controlled neuropsychological investigation of patients with neurotic excoriations.

On the basis of preliminary empirical findings and the pertinent neuropsychological literature the following hypothese are put forward: (1) Because of a certain amount of abreaction (scratching), patients with neurotic excoriations will obtain higher scores in tests of verbal learning and long-term memory than "common' neurotics but probably lower scores in such tests than a group of "normal' subjects. (2) Visual long-term memory is relatively impaired both in patients with neurotic excoriations and in "common' neurotics compared to a "normal' group. (3) Performances IQ for both patient groups mentioned will be relatively low compared to verbal IQ. - The first and second of these suppositions were confirmed but not the third.

Adult↗