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Biomedical subjects

H Neuhaus

Publications and source records attributed to H Neuhaus.

At least 127 records · Page 7Linked to original sources
PubMed1986

[Dose comparative study with ranitidine in the therapy and prevention of duodenal ulcer].

The clinical value of ranitidine, 75 b. d. versus 175 mg b. d. in 48 patients with endoscopically proven duodenal ulcer was evaluated in a randomised double-blind study. In the two groups of patients there was no significant difference of ulcer healing. After 4 weeks of treatment in each group healing of 79% and after 6 weeks of 92% of the ulcers was observed. After 8 weeks the healing rate was 96% in patients who received 75 mg b. d. and 100% in those receiving 150 mg of ranitidine b. d. Smoking prolonged ulcer healing in both groups. Upon ulcer healing in 34 patients a ranitidine dosis of 75 mg nocte for prophylaxis of ulcer recurrence was compared with a 150 mg dosis nocte. Within 12 months in the two groups recurrence of duodenal ulcer was found by endoscopy in 21% and 20% of the patients. 7 out of 8 patients with ulcer recurrence were smokers. According to the results of these studies it appears that the recommended standard dosis of ranitidine for treatment of duodenal ulcer could be reduced by one half. To confirm our conclusions, further studies with a greater number of duodenal ulcer cases are necessary.

Adult↗
PubMed1984

Intervening sequences in the ribosomal RNA genes of Ascaris lumbricoides: DNA sequences at junctions and genomic organization.

An rDNA size class in the genome of the nematode Ascaris lumbricoides is described which is interrupted by a 4.5-kb long intervening sequence located in the 26S coding region. This molecular form occurs in approximately 15 copies per haploid genome and amounts to approximately 5% of the total nuclear rDNA. Intervening sequences are present only in the 8.8-kb rDNA, but not in the 8.4-kb rDNA repeating units of A. lumbricoides. Cloning of the interrupted rDNA units revealed, in addition to the main 4.5-kb insertion, shorter intervening sequences of 4-kb and 119-bp length. Both shorter rDNA forms are present in the single copy range of the haploid genome. Sequence analyses of the intervening sequence/rDNA junctions show an identical right-hand junction for all of the three different rDNA forms. The two shorter intervening sequences are a coterminal subset of the right-hand end of the main 4.5-kb insertion, whereas all three insertions have a different left-hand junction with the coding region of rDNA. Each intervening sequence is flanked by a short direct repeat of variable length, being only once present in the uninterrupted rDNA. The intervening sequences of A. lumbricoides show striking similarity to the organization of type I insertion family in dipteran flies, even though they are inserted at different positions in the 26S coding region. Additional rDNA intervening sequences may be present outside of the rDNA cluster, but in not more than 15-20 homologous copies per haploid genome.

Animals↗
PubMed1982

Nucleotide sequence of satellite DNA contained in the eliminated genome of Ascaris lumbricoides.

Several restriction endonuclease fragments isolated from highly repetitive satellite DNA of the chromatin eliminating nematode Ascaris lumbricoides var. suum have been cloned. Each type of restriction fragment corresponds to a different variant of the same related ancestral sequence. These variants differ by small deletions, insertions and single base substitutions. Restriction and DBM blot analyses show that members of the same variant class are tandemly linked and therefore are physically separated from other variant classes. A comparison of all the determined sequences establishes a 121 bp long and AT rich consensus sequence. There is evidence for an internal short range periodicity of 11 bp length, indicating that the Ascaris satellite initially may have evolved from an ancestral undecamer sequence. The satellite DNA sequences are mostly but not entirely eliminated from the presumptive somatic cells during chromatin diminution. We have no evidence for transcriptional activity of satellite DNA at any stage or tissue analyzed.

Animals↗
PubMed1982

Molecular cloning and sequence analysis of highly repetitive DNA sequences contained in the eliminated genome of Ascaris lumbricoides.

High molecular weight DNA from germ line and somatic cells of the DNA eliminating nematode Ascaris lumbricoides has has been isolated and digested with different restriction enzymes. The resulting DNA fragments were separated by agarose gel electrophoresis. Germ line but not somatic DNA shows a prominent band about 120 bp long as well as multiples of that length. These fragments are shown to be monomers and multimers of a highly repetitive satellite DNA, which is eliminated mostly but not completely during the process of chromatin diminution. Restriction digests, hybridization experiments and sequence analysis revealed that this eliminated satellite is composed of a whole set of different but related variant classes, all of them showing the same repeating unit length of about 120 bp. Members of the same variant class are tandemly linked and therefore physically separated from other variant classes. All satellite sequences can be derived from the same common ancestor sequence, differing only by base substitutions, insertions and deletions. There is no evidence for transcription of satellite DNA at any stage and tissues analyzed.

Animals↗
PubMed1978

Histamine and pancreatitis: increase of plasma histamine levels in dogs with Pfeffer preparation and influence of aminoguanidine on the survival time.

Histamine release could be shown in 50% of the dogs suffering from acute haemorrhagic pancreatitis in Pfeffer's preparation. The survival time of these dogs was shorter by about 50% than that of animals without alteration of the plasma histamine levels. The powerful diamine oxidase blocker aminoguanidine diminished the incidence of severe pancreatitis in the dogs without influencing survival time. As in animals treated by saline histamine release could be shown in 50% of the aminoguanidine-treated dogs in which higher plasma histamine levels were determined than in saline-treated animals. The survival time of the dogs with histamine release, however, was not different from that of animals with normal plasma histamine levels throughtout the experiment. The actions of aminoguanidine in Pfeffer's preparation seemed to be rather complex. Contrasting effects on the development of pancreatitis and on histamine inactivation may have influenced the survival time in different directions.

Acute Disease↗
PubMed1977

The thin-layer isoelectric focusing of lactate dehydrogenase isoenzymes in rabbit lens parts and in intraocular tissues.

Lactate dehydrogenase (LDH) isoenzymes of rabbit lens and other intraocular tissues are separated by thin-layer isoelectric focusing and localized as discrete groups of multiple bands with defined isoelectric points after staining by the tetrazolium method. In the rabbit lens parts, the predominant isoenzymes are LDH-4 and LDH-5. The bands show microheterogeneity, are composed of 2-4 subcomponents, and the pattern shows a distribution of the liver type. The activity of the LDH-4 decreases and that of LDH-5 increases in an order given by the equator, anterior and posterior cortex, and nucleus. LDH-3 remains almost constant in all lens parts. LDH-4 is composed of two subcomponents, one of which, the most cathodic with higher isoelectric point, is almost absent in the lens nucleus. Of the LDH localized in the rabbit intraocular tissues, only the retina shows a pattern of five isoenzymes also of the liver type. In all intraocular tissues LDH-3, -4, and -5 are very prominent, show also microheterogeneity of their isoenzyme bands, and are each composed of 4-6 subcomponents. LDH-1 and -2 show only one isofocused component. Species specificity is shown of the LDH isoenzymes in the rabbit, mouse, dog, and calf lens.

Animals↗
PubMedDate not supplied

Percutaneous cholangioscopic or transpapillary insertion of self-expanding biliary metal stents.

Fifty-two self-expanding metal stents were implanted in 39 patients with malignant (35 patients) or benign (4 patients) biliary stenoses. The stents were inserted and properly released by means of a 7 or 9 French gauge delivery catheter via the percutaneous (20 patients) or transpapillary (19 patients) route. In all cases the endoprostheses expanded to a diameter of 7 to 10 mm and achieved complete biliary tract drainage. Jaundice disappeared in 36 of the 39 patients. No early complication was observed. After a median follow-up of 121 days (range, 30 to 422 days), 19 of 36 patients are still alive and 17 died of non-procedure-related causes. Biliary re-obstruction occurred in five patients due to tumor overgrowth above or below the prosthesis (four patients) or bile encrustation (one patient). In patients with malignant stenoses, the probability of stent patency is 78% after 200 days. We conclude that large-bore metal stents are safe, effective, and provide better long-term patency than conventional endoprostheses.

Adenoma, Bile Duct↗
PubMedDate not supplied

Cholangioscopy.

Explore the source record for details and available documents.

Biliary Tract Diseases↗
PubMedDate not supplied

Intrahepatic stones: the percutaneous approach.

Intrahepatic stones are prevalent in the Far East, whereas they are infrequently seen in Western countries. Hepatolithiasis can cause recurrent attacks of cholangitis, with a risk of liver abscesses, sepsis or hepatic failure. Immediate biliary decompression can usually be achieved by endoscopic or percutaneous transhepatic drainage. Definitive treatment should aim for complete elimination of bile stasis and removal of all stones. Hepatic resection promises the best long term results when the disease is limited to segments or the left liver lobe. Endoscopic retrograde choledochopancreatography is not well established for intrahepatic stones because of frequent failures due to associated biliary strictures, angulated ducts or peripherally impacted concrements. In contrast, percutaneous procedures can be easily performed through a T tube tract for residual stones after surgery. Establishment of a transhepatic fistula allows a targeted approach to liver segments with catheters or miniscopes, without the need for laparotomy. Biliary strictures can be dilated with balloons, and intrahepatic stones can be removed with baskets under fluoroscopic or cholangioscopic control. These techniques can be combined with electrohydraulic lithotripsy or laser lithotripsy for disintegration of impacted calculi. The risk of stone recurrence is particularly high in patients with associated biliary stenoses. Temporary or long term transhepatic intubation is a promising approach in these cases. The optimal management of intrahepatic stones remains a challenging task that requires an experienced team of gastroenterologists, surgeons and radiologists.

Bile Ducts, Intrahepatic↗
PubMedDate not supplied

Treatment of an esophagorespiratory fistula by insertion of an esophageal Montgomery and tracheal dynamic stent after failure of conventional endoprosthesis.

Esophagorespiratory fistulae at the adult age can develop through malignant tumor growth, endoscopy, bougienage, laser therapy, or through a radiochemotherapy. We report a female patient with inoperable bronchial cancer, who developed a symptomatic esophagorespiratory fistula during radiochemotherapy with cisplatin. At first, conventional plastic tubes and then novel selfexpanding silicone-coated Gianturco-Song stents were used in an unsuccessful attempt to close the fistula. After the extraction of two Gianturco-Song stents, the insertion of a Montgomery-Salivary bypass stent in the esophagus and a dynamic stent in the trachea resulted in a permanent occlusion of the fistula. This case demonstrates that Montgomery-Salivary bypass stents do not tend to migrate due to their characteristic shape and self-fixation, and that the novel self-expanding, silicone coated Gianturco-Song stents can be extracted with rigid endoscopy if necessary.

Adult↗
PubMedDate not supplied

[Endoscopy in the diagnosis and treatment of acute obstructive pancreatitis].

Opie's theory that obstruction of the Ampulla of Vateri can be a significant factor in acute pancreatitis, is supported by various clinical observations. The significance of further pathogenetic factors such as anatomic variance of the duct systems, pressure differences, direction of flow and composition of secretions will have to be established in future studies. The ERCP plays an important role as a safe and sensitive diagnostic procedure. Therefore, ERC should be carried out in all suspected acute obstructive pancreatitis cases. The appropriate selection of patients employing clinical and laboratory criteria and other imaging procedures should, in the future, be defined more accurately. Presently, in proven ampullary obstruction we always perform immediate endoscopic therapy. However, the gallstone-induced obstructive pancreatitis usually has a good recovery course, even with conservative management. Therefore, clinical studies should prospectively direct us to those cases, in which the invasive endoscopic procedure should be limited, i.e. patients with expected serious courses. Diagnostic and therapeutic endoscopy does not increase the morbidity and mortality of the acute obstructive pancreatitis. Unlike the surgical approach, the endoscopic procedure is less invasive and is preferred by patients.

Acute Disease↗
PubMedDate not supplied

Endoscopic lithotripsy of bile duct stones using a new laser with automatic stone recognition.

Biliary stones can be removed in 85% to 90% of patients using endoscopic sphincterotomy; in the rest alternative methods are required. Thirty-eight consecutive patients in whom conventional methods had failed underwent laser lithotripsy with a new laser system. A flashlamp-pumped pulsed laser with rhodamine 6G as dye (594 nm) has a tissue-stone recognition system that can identify bile duct stones by analyzing backscattered light and interrupt the pulse in case of tissue contact (Lithognost, Telemit, Munich, Germany). Access of the 0.25- or 0.30-mm-diameter laser fiber to the stones was achieved perorally in 18 patients. In 13 of these cases, eccentrically located stones in the middle or proximal common bile duct were targeted with a 3.4-mm miniscope introduced through a standard duodenoscope. Fluoroscopically guided peroral lithotripsy was performed in 5 patients with stones in the distal common bile duct that could be approached with a standard ERCP catheter. Percutaneous cholangioscopic laser lithotripsy was carried out in 20 patients with stones not amenable to retrograde techniques. The mean number of bile duct stones per patient was 3.6, and the average diameter of the largest stone of each patient was 25 mm (range, 8 to 52 mm). The bile ducts were cleared in all but 1 patient in a mean number of 1.3 sessions lasting 15 to 115 minutes (mean, 60). No laser-related complications were observed. The Lithognost laser was successfully used in 37 of 38 patients referred for the removal of difficult bile duct stones.(ABSTRACT TRUNCATED AT 250 WORDS)

Aged↗