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Biomedical subjects

H Murata

Publications and source records attributed to H Murata.

At least 325 records · Page 18Linked to original sources
PubMed1985

The histochemistry of asparagine-linked oligosaccharides of glycoproteins in mammalian and avian tissues as studied by digestion with almond glycopeptidase.

In a series of mammalian and avian tissues, asparagine-linked oligosaccharides of glycoproteins have been localized histochemically by means of combined periodic acid-Schiff (PAS) and almond glycopeptidase digestion procedures. According to the present results, the particular saccharide residues were found to be localized primarily in glycoproteins of connective and muscular tissue elements but were hardly visualizable or absent in glycoproteins of epithelial tissue elements. In view of the substrate specificity of almond glycopeptidase and the known staining selectivity of the PAS reaction, the majority of connective and muscular tissue elements are thought to be the main sites where plasma types of glycoproteins are involved.

Amidohydrolases↗
PubMed1985

[Three-dimensional image processing for analysis of ventricular wall motion].

Three-dimensional data processing was developed for single photon computed tomographic imaging of the multigated blood pool with Tc-99m labelled red blood cells. A gamma camera (ZLC-7500; Siemens) was rotated from the left posterior oblique (LPO 45 degrees) to the right anterior oblique (RAO 45 degrees) projections at intervals of 180 degrees/32. Data were collected at each part for one to two minutes, and the number of gates per beat was 12 to 20, depending on the patient. Three-dimensional transaxial images of the heart were reconstructed by the filtered back projection method without attenuation correction. A Shepp & Logan filter was used for the convolution filter. Short-axial images were reconstructed from the transaxial images. Using the equicount level method, the contours of both ventricles at each short-axial cross-section image were determined automatically with 50% levels of the maximum counts. Each contour line was smoothed to the fifth order of Fourier function after conversion from (x-y) coordinates to (r-theta) polar coordinates with respect to the center of the area. Each contour line, converted again into (x-y) coordinates, was drawn successively on a color CRT at constant intervals and inclinations in order to display the ventricles three-dimensionally. It is possible to display simultaneously on a CRT the right and left ventricles or two types of cardiac disease. It is also possible to depict superimposed images of end-diastolic and end-systolic ventricles. Three cases including a normal control, one with old inferior infarction and one with hypertrophic cardiomyopathy (HCM) were examined.(ABSTRACT TRUNCATED AT 250 WORDS)

Cardiomyopathy, Hypertrophic↗
PubMed1985

[Ileal artery aneurysm: a case report and review of literature].

In a 70-year-old Japanese male who had a history of a radical operation of ruptured descending thoracic aortic aneurysm, the ileal artery aneurysm developed asymptomatically during postoperative follow up period. The aneurysm was about 10 by 4.5 by 4 cm in size and atherosclerosis microscopically. This case seemed to be the 20th case of superior mesenteric artery aneurysm and the 4th case of ileal artery aneurysm reported in Japan. Furthermore, this may be the first report of ileal artery aneurysm associated with thoracic aortic aneurysm in Japanese and English literature.

Aged↗
PubMed1984

Rapid inactivation of Ca2+, Mg2+-dependent endonuclease of rat liver nuclei after cycloheximide treatment.

Ca2+, Mg2+ dependent endonuclease activity of isolated nuclei from rat liver disappeared completely within one to two hours after intraperitoneal administration of inhibitors of eukaryotic protein synthesis such as cycloheximide or puromycin. Actinomycin D, on the other hand, revealed no inhibition of the endonuclease activity, but even reversed the effect of cycloheximide by simultaneous addition.

Animals↗
PubMed1984

Effects of digestion with N-oligosaccharide glycopeptidase upon certain lectin-peroxidase-diaminobenzidine reactions of glycoproteins in mammalian and avian tissues.

To determine the histochemical localization of asparagine-linked oligosaccharides of glycoproteins in a series of different mammalian and avian tissues, the effects of digestion with N-oligosaccharide glycopeptidase upon certain lectin-peroxidase-diaminobenzidine reactions of the histological structures involved have been studied by light microscopy. Throughout the tissues examined, asparagine-linked oligosaccharides of glycoproteins were localized mainly in histological structures of connective and muscular tissues, but were hardly or not visualized in those of epithelial tissues. These results appear to lead to the concept that connective and muscular tissues represent the main sites where plasma types of glycoproteins are involved in mammalian and avian species.

3,3'-Diaminobenzidine↗
PubMed1984

Colchicine-induced alteration of hormone-stimulated cyclic AMP synthesis in pig skin (epidermis).

Effects of colchicine on the epidermal adenylate cyclase systems were investigated. When pig skin (epidermis) was incubated in RPMI 1640 medium without the addition of serum, the beta-adrenergic adenylate cyclase response (epinephrine-induced cyclic AMP accumulations) gradually decreased, whereas adenosine and histamine responses remained high or increased during the long-term (up to 48 h) incubation period. The addition of colchicine (1 mumol/liter) in the incubation medium resulted in an increase in the beta-adrenergic responsiveness and a decrease in adenosine and histamine responsivenesses. The effects of colchicine were both time- and concentration-dependent; they could be observed after 9-12 h incubation, and the maximal effect was obtained at a concentration of 0.1 mumol/liter. Similar effects were observed by the addition of another microtubule-disruptive agent, vinblastine. On the other hand, cytochalasin B, which affects the microfilament system, apparently decreased the beta-adrenergic response and increased adenosine and histamine responses during the long-term incubation period. The addition of serum in the incubation medium resulted in essentially the same effect as that of colchicine; in the presence of serum, colchicine-treated skin responded much more markedly to epinephrine (and much less to adenosine and histamine) than the control skin after 24- and 48-h incubation. Previously we reported that hydrocortisone has similar potentiating effects on the beta-adrenergic system of epidermis. The comparison of the effects of both compounds revealed that colchicine had a stronger effect than hydrocortisone, and furthermore, the simultaneous addition of both compounds (colchicine and hydrocortisone) in the incubation medium resulted in the more marked increase of beta-adrenergic response than the single addition of each chemical. Our overall results, coupled with the finding that hydrocortisone has no toxic effects on the adenosine- or histamine-adenylate cyclase system of epidermis, suggest that colchicine affects epidermal adenylate cyclase systems probably through a mechanism that is independent of glucocorticoid (hydrocortisone) effect.

Adenosine↗