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Biomedical subjects

H Mizoguchi

Publications and source records attributed to H Mizoguchi.

At least 469 records · Page 26Linked to original sources
PubMed1980

Effect of peripheral blood mononuclear cells from aplastic anemia patients on the granulocyte-macrophage and erythroid colony formation in samples from normal human bone marrow in vitro--a cooperative work.

Thirty-six patients with aplastic anemia and three with aplastic anemia-PNH syndrome in eight institutes were studied for the presence of peripheral blood mononuclear cells having a suppressive effect on granulocyte-macrophage (G-M) and erythroid colony formation using a uniform protocol. In 11 cases of 29 (38%) and 9 cases of 29 (31%), the presence of mononuclear cells with a suppressive effect on G-M and erythroid colony formation, respectively, was demonstrated. However, the presence of mononuclear cells suppressive both to G-M and erythroid colony formation was demonstrated only in 3 of 19 cases (16%).

Adolescent↗
PubMed1980

A new technique for the cytochemical examination of human hemopoietic cells grown in agar gel.

In this paper, a new technique for the cytochemical examination of human hemopoietic cells grown in agar gel is described. All colonies in the agar gel in a plastic dish can be observed as a whole plate preparation after being stained cytochemically. Granulocytes and monocytes/macrophages in a cluster or a colony were easily distinguished from each other by esterase activity in response to naphthol AS-D chloroacetate of alpha-naphthyl acetate. To illustrate this technique's possibilities a time course study on the formation of granulocyte and monocyte/macrophage clusters or colonies from normal marrow cells was performed.

Agar↗
PubMed1979

gamma-Glutamyl DOPA and gamma-glutamyl dopamine: effect on plasma glucose levels.

gamma-Glutamyl-L-3,4-dihydroxyphenylalanine (gamma-glutamyl DOPA) and gamma-glutamyldopamine (gamma-glutamyl DA) are kidney specific prodrugs. Their effect on plasma glucose levels in the rat was compared to that of L-DOPA and dopamine (DA) after a 30 min intravenous infusion. L-DOPA and DA induced hyperglycemia after 15 min of druginfusion. A more marked and protracted elevation of plasma glucose was observed after infusion of gamma-glutamyl DA. By gamma-glutamyl DOPA had no effect on plasma glucose levels in spite of the high accumulation of DA in the pancreas after this prodrug. Of the various dopamine produrgs studied only gamma-glutamyl DOPA was not hyperglycemic in doses that are known to increase renal plasma flow in the rat. A simplified new procedure for the synthesis of gamma-glutamyl DA is described.

3,4-Dihydroxyphenylacetic Acid↗
PubMed1979

An improved plasma culture system for the production of megakaryocyte colonies in vitro.

A modified culture system has been developed to grow and quantitate megakaryocyte colonies from mouse bone marrow more efficiently than described in other reports. Using this method, it was shown that 30% of CFU-M in normal marrow cells and 70 to 90% of CFU-M in regenerating marrow cells were killed by high specific activity 3H-TdR in vitro. These results indicate that CFU-M are proliferating even in normal adult hemopoietic tissue, but that the proportion of cells that are proliferating is greater in regenerating marrow than in normal intact mice.

Animals↗
PubMed1978

Serial studies on in vitro colony formation in patients with acute leukemia in relation to the maintenance of remission.

For the purpose of preventing a relapse of acute leukemia which is currently the major problem in the successful treatment of the disease, repeated consolidation or intensification therapy during the first year following remission is important. To evaluate these therapies, we investigated the serial changes in CFU-C's of the marrow cells from 12 patients with acute nonlymphocytic leukemia in remission and tried to estimate the relationship between the intensity of consolidation or intensification therapy and the duration of remission, utilizing the degree of reduction in CFU-C's seven days after these treatments as an indicator. As a result, after 21 out of 22 courses of therapy where CFU-C's were reduced significantly after the therapy, the patients were still in remission at the time of the next intensificiation therapy (at most for about 100 days). On the other hand, after five out of ten courses where CFU-C's were not reduced significantly, the patients were in relapse at the time of the next intensification therapy. From these results, it may be inferred that cases whose CFU-C's are not reduced significantly should be treated intensively again within a short period.

Acute Disease↗
PubMed1978

A case of intrarenal artery stenosis associated with erythrocytosis.

A case of erythrocytosis with increased plasma erythropoietin level was reported. Peripheral leucocytes and thrombocytes were normal. No splenomegaly was detected. Studies to find underlying disorders causing increased plasma erythropoietin level and erythrocytosis revealed no abnormalities except for the thickened and tortuous interlobular and afferent arteries in the kidney. This change was suggested as the cause of erythrocytosis found in this case.

Adult↗