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Biomedical subjects

H Miyamoto

Publications and source records attributed to H Miyamoto.

At least 253 records · Page 14Linked to original sources
PubMed1994

Oral and maxillofacial reconstruction using the free rectus abdominis myocutaneous flap. Various modifications for reconstruction sites.

The rectus abdominis myocutaneous flap consists of the skin paddle, anterior rectus sheath, rectus muscle and deep inferior epigastric artery and veins. Of these four components, the skin paddle, rectus sheath and rectus muscle could all be adjusted appropriately for reconstruction sites. Only one or two large perforators in the paraumbilical region could easily supply the large abdominal wall. The bulkiness of the rectus abdominis flap is reduced by removal of subcutaneous fat and rectus muscle. These possibilities can provide many modification of the flap for various reconstruction sites. 16 cases were reconstructed primarily and secondarily using this flap after oral and maxillofacial tumour ablation. The results of these cases indicated a very favourable and reliable vascularity. The rectus abdominis myocutaneous flap was considered to be one of the most suitable flaps for oral and maxillofacial reconstruction. Various modifications for different reconstruction sites are mainly discussed.

Adipose Tissue↗
PubMed1994

A case of the 'dapsone syndrome'.

The 'dapsone syndrome' developed in a 44-year-old woman who was treated for pyoderma gangrenosum with 100 mg/day dapsone for about 5 weeks. Symptoms included fever, malaise, jaundice with hepatic dysfunction, lymphadenopathy, mononucleosis and dermatitis. These symptoms disappeared with 30 mg/day of oral prednisolone. A lymphocyte stimulation test with dapsone was positive, as was the delayed-type intradermal skin test with 0.5 and 0.05% dapsone in saline. Immunohistochemical studies of the rash and skin test reaction revealed that the dominant infiltrating T cells in the upper dermis were of the Leu 2a+ cytotoxic/suppressor-type rather than the Leu 3a+ helper/delayed hypersensitivity-type.

Adult↗
PubMed1994

Successful cardiac presrevation for 12 hours using nondepolarizing cold cardioplegia. A canine model.

Isolated canine hearts were preserved for 12 h at 5 degrees C followed by normothermic reperfusion for 2 h. Dogs were divided into two groups: group 1 (n = 7) received a nondepolarizing preservation solution in multidose, and group 2 (n = 6) received single-flushed University of Wisconsin (UW) solution, both administered in multidose fashion. At the end of reperfusion, the myocardial adenosine triphosphate concentration and left ventricular systolic and diastolic function were preserved better in groupl than in group 2. Myocardial mitochondrial ultrastructural integrity was identical in the two groups. These results suggested that in a 12-h heart preservation, nondepolarizing solution administered in multidose fashion protects the myocardium from the deleterious effects of hypothermia and cardioplegia better than UW solution.

Adenine Nucleotides↗
PubMed1994

Two acidic polysaccharides having reticuloendothelial system-potentiating activity from the raw root of Rehmannia glutinosa.

Two acidic polysaccharides, called rehmannan FS-I and rehmannan FS-II, were isolated from the raw root of Rehmannia glutinosa LIBOSCHITZ. They were homogeneous on electrophoresis and gel chromatography, and their molecular masses were estimated to be 5.8 x 10(4) and 6.6 x 10(4), respectively. Rehamannan FS-I is composed of L-arabinose: D-galactose: L-rhamnose: D-galacturonic acid: D-glucuronic acid in the molar ratio of 68:40:4:84:3; rehmannan FS-II is composed of L-arabinose: D-galactose: L-rhamnose: D-galacturonic acid in the molar ratio of 18:15:4:33, in addition to small amounts of O-acetyl groups. About two-thirds (rehmannan FS-I) and about one half (rehamannan FS-II) of the hexuronic acid residues exist as methyl esters. Methylation analysis of the carboxyl-reduced derivatives and nuclear magnetic resonance studies indicated that their structural features include mainly both arabino-3,6-galactan and rhamno-galacturonan type structural units. Both polysaccharides showed remarkable reticuloendothelial system-potentiating activity in a carbon clearance test.

Animals↗
PubMed1994

Structural features and anti-complementary activity of rehmannan SA, a polysaccharide from the root of Rehmannia glutinosa.

The structural features of rehmannan SA, a polysaccharide with remarkable reticuloendothelial system-potentiating activity obtained from the root of Rehmannia glutinosa, were investigated by methylation analysis and periodate oxidation. Rehmannan SA is mainly made up of arabino-3,6-galactan type structural units. Both rehmannan SA and rehmannan SB showed pronounced anti-complementary activity.

Animals↗
PubMed1994

Characterization of two polysaccharides having activity on the reticuloendothelial system from the root of Rehmannia glutinosa.

Two acidic polysaccharides, called rehmannan SA and rehmannan SB, were isolated from the dried root of Rehmannia glutinosa Liboschitz. They were homogeneous on electrophoresis and gel chromatography, and their molecular masses were estimated to be 6.4 x 10(4) and 7.9 x 10(4), respectively. They were commonly composed of L-arabinose:D-galactose:L-rhamnose:D-galacturonic acid in the molar ratios of 10:10:1:1 (rehmannan SA) and 14:7:3:8 (rehmannan SB), in addition to small amounts of peptide moieties. About eighty percent (rehmannan SA) and about thirty percent (rehmannan SB) of the hexuronic acid residues exist as methyl esters. Reduction of carboxyl groups, methylation analysis, nuclear magnetic resonance and the controlled Smith degradation studies indicated the structural features of rehmannan SB, the major one in terms of the yield. It has a core structural unit of a characteristic rhamnose-rich arabino-3,6-galactan type. Both polysaccharides showed remarkable reticulo-endothelial system-potentiating activity in a carbon clearance test.

Animals↗
PubMed1994

Immunohistochemical microquantitation method for type I collagen in kidney histological section of the rats.

An immunohistochemical micromethod for quantitation of type I collagen in frozen histological section of rat kidney was developed. A principle of this method is enzyme-linked immunosorbent assay (ELISA). The intensity of immunoreactivity of the section to an anti-type I collagen antibody was quantitated, and then the total protein content in the same section was measured colorimetrically. Type I collagen content was expressed as mg of type I collagen per g of total protein. In this micromethod, the minimum area and optimum thickness of the section were 5 mm2 and 10 microns, respectively. Type I collagen content in the rat kidney section was 3.02 +/- 0.12 mg/g. This method should be useful for diagnosis of renal failure.

Animals↗
PubMed1994

Unilateral anophthalmia in a Holstein calf.

Unilateral anophthalmia in a Holstein calf was examined morphologically. On the left side, a small cystic remnant of the eye ball (CRE) was present in the orbit. The CRE was adhered to the dura mater through an abnormal foramen formed in the orbital region of the cranial base. At this region of the left cerebral hemisphere, a porencephalic change was detected. Histologically, the pigment epithelium was continued from the lining of the inner surface of CRE to the residual cerebrocortical tissue attached to the dura mater. These characteristic changes seem to suggest the partial fusion of the outer layer of the optic cup to the telencephalon during optic organogenesis.

Animals↗
PubMed1994

A new immunohistochemical method for quantification of fast-myosin in frozen histologic sections of the rat skeletal muscles.

An immunohistochemical micromethod for quantification of fast-myosin in frozen histologic sections of rat skeletal muscles was developed. The principle of this method was enzyme-linked immunosorbent assay (ELISA). We used frozen tissue sections as models of the antigen-coated wells of ELISA plate. The intensity of immunoreactivity of the frozen section to an anti-fast-myosin monoclonal antibody was quantified directly from the color developed with the second antibody coupled with peroxidase using phenol-4-aminoantipyrine as a substrate. Then, the same section was incubated in 0.01 M acetic acid solution to cleave antigen-antibody complexes, followed by colorimetric measurement to obtain the absolute value of total protein per section. Fast-myosin content in the frozen tissue section was expressed as mg of fast-myosin per g of total protein. In this micromethod, the minimum area and the optimum thickness of the section were 5 mm2 and 10 microns, respectively. Fast-myosin contents in the extensor digitorum longus and soleus muscles were 185.0 +/- 6.1 and 17.5 +/- 2.4 mg/g, respectively. The results obtained by this micromethod were in good agreement with those obtained by two conventional methods, immunohistochemical morphometry and biochemical determination. This micromethod is useful for a quantitative evaluation of the contractile function of the mammalian skeletal muscles.

Actins↗
PubMed1994

Steroid injection therapy for pseudocyst of the auricle.

In our clinic, we encountered 8 patients with pseudocyst of the auricle during a 6-year period from 1987 to 1992 and injected steroid solution locally into the pseudocysts. Three of these patients had no recurrences and 4 were treated successfully after 1-3 recurrences. In only one case was this therapy ineffective. After 11 injections, which resulted in permanent deformity of the auricle, the patient underwent surgery. We believe that local steroid injection therapy should be the first choice method for treating auricular pseudocysts. However, frequent injections can cause auricular deformity and if there are more than 3 recurrences, the pseudocyst should be managed surgically. The lactate dehydrogenase levels of the cystic fluid were determined in 3 of the 8 patients and proved high; in only one patient was the etiology of the pseudocyst thought to be associated with minor trauma.

Adolescent↗
PubMed1994

Phosphorus-31 nuclear magnetic resonance study of energy metabolism in intact slow- and fast-twitch muscles of rats.

The time course of the catabolism of phosphorylated metabolites was studied by phosphorus-31 NMR(31P NMR) spectroscopy over a 6-h period after the isolation of rat slow- and fast-twitch muscles, the soleus and the extensor digitorum longus (EDL), respectively, obtained without any muscle damage. In both muscles, rapid depletion of creatine phosphate was followed by a decrease in ATP. These high-energy phosphates disappeared earlier in the soleus than in the EDL. In both muscles, inorganic phosphate (Pi) largely increased in a biphasic pattern, and sugar phosphates (SP) also showed considerable increase. The NMR-visible total phosphates (NTP) increased significantly in the soleus during the latter stage of observation. This increase in NTP was attributed to an increase in Pi. Although the two muscles showed the same initial (7.21) and final (approximately 5.9) intracellular pH (pHi), the pattern of pHi decline differed between these muscles. The rapid fall of pHi in the soleus in the early stage suggests that nonlactic acid acidosis plays a significant role in postmortem changes.

Animals↗
PubMed1994

[History of the syndromes of Yin (retention of body fluids) - the relationship between the syndromes of Yin in Chinese medicine and those of Yin in Buddhist medicine].

We traced the history of the term "yin" by means of Chinese medical books and classical Chinese Buddhist literature. As a result of our study, the following points became clear. The syndromes of yin described in the Shan han za bing lun are divided into two groups. Those of the first group are characterized by short retention of body fluids. The yu yin is representative of this group. On the other hand, those of the second group are distinguished by long retention of body fluids. The tan yin is typical of this group. It was deduced that the former group originated in Chinese traditional medicine because it is found in the Su wen and the Ling shu. The latter is supposedly derived from Buddhist medicine because it is similar to the syndromes of yin in Buddhist medicine.

Body Fluids↗
PubMed1994

Change in density of K+ current of HeLa cells during the cell cycle.

We investigated the cell cycle-dependency of the channel, using cells synchronized by harvesting them at the mitotic stage. The inward K+ currents by whole-cell patch-clamp were highest in early G1, and then decreased with time to a minimum in the S phase, increasing again in the M phase. In contrast, membrane surface area increases with progression of the cell cycle, whereas the currents decreases from early G1 to an apparent minimum in the S phase. Therefore, the densities of K+ current at fixed membrane potentials were highest in early G1 phase and lowest in the S phase.

Calcium↗
PubMed1994

Blood flow velocity in common carotid artery in humans during breath-holding and face immersion.

Blood flow velocity was measured in the common carotid artery of humans during breath-holding (BH) in the supine and upright positions, and during breath-hold face immersion (FIBH) in cold (20 degrees C) and warm (35 degrees C) water in the upright position with 90 degrees flexion of the upper body, to test the influences of the body position, facial immersion and temperature on changes in cerebral blood flow. Simultaneously, the heart rate (HR) and blood pressure (BP) under the same conditions, and the end-tidal PO2 and PCO2 (PETO2 and PETCO2) during supine BH, were also measured. Results showed that posture had no significant effect on the apneic HR. Bradycardia developed during cold FIBH, but not warm FIBH. The BP increased during BH in both positions, and during both cold and warm FIBH. The PETO2 decreased and the PETCO2 increased significantly at the end of BH. The blood velocity decreased transiently at the beginning of upright BH, but increased time-dependently during both supine and upright BH. Moreover, the velocity increased more during warm FIBH than during BH's, and even more during cold than warm FIBH. When bradycardia occurred during cold FIBH, the velocity integral per minute tended to decrease. These results suggest that the cerebral blood flow increases in these conditions of BH. This increase can be explained by the increase in PETCO2 and decrease in PETO2, possibly with a slight contribution from the increase in the BP.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1994

[Application of the polyglycolic acid (PGA) pledgets or sheet for pulmonary fistulas and defects of the pleura].

A felt-like bioabsorbable prosthesis (Polyglycolic Acid (PGA) pledget and sheet; Medifit feft) was used for pulmonary fistula in a total of 10 patients. "Fibrin glue A" was absorbed into the PGA prosthesis previously. And next, the part of pulmonary fistula was coated with "Fibrin glue B", and then covered with the fibrin-absorbed PGA prosthesis. The prosthesis adhered to the diseased part of pulmonary pleura immediately, and therefore, in all these 10 patients, the post-operative air-leakage was stopped up to 4 days and neither side effect nor complication was observed.

Adult↗
PubMed1994

Comparative study of cutaneous T-cell lymphoma and adult T-cell leukemia/lymphoma.

An important disease entity distinct from cutaneous T-cell lymphoma (CTCL) in Japan is adult T-cell leukemia/lymphoma (ATL), which shows almost the same phenotype as CTCL, ie, a helper/inducer T-cell phenotype (CD4-positive, CD8-negative), and usually involves the skin. This article describes differences between CTCL and ATL in terms of clinical and immunopathologic cell surface features. In patients with ATL, the predominant physical findings were lymph node, bone marrow and skin involvement, hepatosplenomegaly, leukemic manifestations, and an aggressive course. In patients with CTCL, in contrast, only skin lesions predominated at the onset of the disease and a relatively good prognosis was shown. The predominant phenotype of the neoplastic cells in the skin of patients with CTCL was CD3+, CD4+, CD29+, CD45RO+, HLA-DR+, HLA-DQ+, CD7-, L-selectin-, and CD45RA-. Some phenotypic discrepancy was found between the neoplastic cells in the peripheral blood, lymph nodes and skin of patients with ATL with respect to CD45RA and CD45RO, and CD7, CD29, CD25, and HLA-DR. That is, the predominant neoplastic cell phenotype was helper T-cell, which was CD3+, CD4+, L-selectin+, CD25+, CD45RA+, HLA-DR+, CD29-, and CD45RO- in peripheral blood, and CD3+, CD4+, L-selectin+, CD29+, CD45RO+, HLA-DR+, and CD45RA- in the skin and lymph nodes. Phenotypic heterogeneity of ATL cells and heterogeneity of CD45R isoform expression on ATL cells were evident in different organs. These findings confirm that the difference in antigen expression on the cell surface might reflect the clinical features of ATL and CTCL. CTCL cells do not share the same phenotype as ATL cells.

Adult↗