Biomedical subjects
Publications and source records attributed to H Mitsui.
Thirty patients with atlanto-axial subluxation were operated on using the Mitsui Memorial Hospital System. The follow-up averaged one and a half years, and the results were satisfactory in comparison with other operative methods, all patients regaining almost normal function. Stability and safety are the main advantages of this procedure, with almost complete correction of subluxation in most patients.
SGP140 glycoprotein, a major cell surface sialoglycoprotein with an apparent m.w. of 140,000, was detected on the human T lymphoblastoid cell line P12/Ichikawa by labeling with periodate-tritiated sodium borohydride, followed by urea-sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fluorography. Then SGP140 was purified from P12/Ichikawa cells for study of its biochemical character and its distribution in various cell lines. The purification was performed by 0.2% Triton X-100 solubilization from crude membranes, DEAE-Sephacel column chromatography, WGA-agarose column chromatography, Blue-Sepharose 6MB column chromatography, and Sephadex G-150 gel filtration. Antiserum raised against SGP140 was then prepared, and immunoprecipitation and membrane immunofluorescence assay were performed on various cell lines. SGP140 was detected on P12/Ichikawa, Raji, P3HR-1, Daudi, Namalva, BALL-1, MOLT-4B, TALL-1, NALL-1, and K562 cells, but was not detected on HL-60 cells. When HL-60 cells were treated with dimethyl sulfoxide, retinoic acid, or 12-O-tetradecanoylphorbol-13-acetate, SGP140 was detected on cell surfaces. We discuss the possibility that SGP140 may be a differentiation antigen.
Transformation of rat cells by avian sarcoma viruses (ASVs) induced the release of transforming growth factors (TGFs), which transiently promote anchorage-dependent untransformed BALB3T3 clone A31 cells to form progressively growing colonies in soft agar, into serum-free conditioned medium. A new class of TGF, which is a heat and acid-labile protein with a molecular mass of 12,000 daltons, was isolated and purified from an ASV-transformed rat cell line, 77N1, derived from NRK cells. The TGF from 77N1 did not bind to epidermal growth factor membrane receptors. We observed specific in vitro phosphorylation of several membrane proteins from A31 cells in the presence of the TGF.
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The effect of retinoids (Rds) on cell proliferation was studied in serum-free culture condition, using non-transformed and transformed derivatives of BALB 3T3. Cell proliferation of an SV40-transformed line was inhibited significantly by Rd treatment. However, proliferation of two cell lines that were transformed by a Kirsten and Moloney strain of murine sarcoma virus (MSV) and produced growth factor into culture medium, was remarkably stimulated by Rds. Addition of serum masked both the inhibitory and stimulatory effects of Rds.
The absorption of doxycycline (DOTC), bekanamycin (AKM), cefazolin (CEZ), penicillin G (PC-G), prednisolone, and human IgG through the rabbit's tracheal mucosa was examined using the double-chamber method. The results indicate that the tracheal mucosa can absorb both small molecules and macromolecules. Passing through the intercellular space, DOTC, a small molecule, was absorbed through the tracheal epithelium by diffusion. Human IgG, a macromolecule, was absorbed by the mechanism of an energy-consuming transport process. This study of the absorption of drugs through the respiratory mucosa could open up new areas in the utilization of intrarespiratory administration of drugs and lead to a better understanding of the mechanisms involved in respiratory allergies.
Motility, adhesiveness, IL1 production, and inhibition of tumor cell growth in vitro were examined in murine peritoneal macrophages obtained after intraperitoneal injection of a streptococcal preparation OK-432, heat-inactivated OK-432 (HI-OK-432), and thioglycollate medium (TG). By varying the interval between intraperitoneal injection of OK-432 and the harvest of peritoneal macrophages, it was found that OK-432 induced a time-dependent multi-step alteration of these properties: step I increased motility on day 1: step II increased adhesiveness on day 2; and step II increased inhibition of tumor cell growth and IL1 production. During step III, the peritoneal macrophage population, including Ia-bearing cells, increased dramatically in the peritoneal cavities of OK-432-treated mice. In contrast, injection of either HI-OK-432 or TG, which lack antitumor activity in vivo, initiated steps I and II, but not step III. The Ia-bearing macrophages induced by OK-432 showed high ability of IL1 production, but low growth inhibitory activity against tumor cells. Based on these results, OK-432 seems to be performing a dual function: eliciting a new population of macrophages to the site of injection (heat stable function), and inducing two different populations of antitumor macrophages and Ia-bearing macrophages (heat unstable function).
Transformation of rat cells by avian sarcoma viruses induced the release of growth factors into serum-free conditioned medium. An avian sarcoma virus-transformed rat cell line, 77N1, produced and released a polypeptide growth factor, classified as a transforming growth factor (TGF), which transiently promotes anchorage-dependent BALB3T3 A31 cells to form progressively growing colonies in soft agar. The TGF was isolated and partially purified from an extract of 77N1 cells by ion-exchange chromatography on a diethylaminoethyl Sephacel column followed by ammonium sulfate precipitation. The TGF was assumed to have a molecular weight of 11,000 from gel filtration on Sephadex G-50. This TGF did not compete with epidermal growth factor for binding to cell membrane receptors and was not potentiated by epidermal growth factor. The TGF was trypsin and dithiothreitol sensitive as well as heat and acid labile, indicating that it was different from previously reported TGFs of similar molecular weight and thus belonged to a new class of TGFs.
Seventy one patients with renal tumors treated at our clinic during the 11 years from 1970 to 1980 were clinically examined. The results are summarized as follows. The frequency of patients with renal tumors was 0.22% of the outpatients and 1.72% of the inpatients. Of the 71 renal tumors, 41 were renal adenocarcinoma, and 26 were renal pelvic tumors of which 23 were transitional cell tumors, 2 were squamous cell tumors, and 1 was adenocarcinoma. The other tumors were 1 adenoma, 1 hemangioma, 1 hematoma, and 1 foreign body granuloma. The right and left kidneys were affected at equal frequencies. Male patients were more commonly affected, the sex ratio being 39 to 32. The youngest case was a 29-year-old female, and the eldest was a 84-year-old male. As the initial symptoms and chief complaints, gross hematuria was most frequent (52 cases, 73.2%), followed abdominal tumor mass (32 cases, 45.1%), and fever (26 cases, 36.6%). Only 2 cases showed the classic triad, while 1 case had none of them. The period between onset of symptoms and admission, was within 1 year for all patients except for 2 cases. Metastasis was found in 52 cases. The lung was the most frequent site of metastasis (12 cases, 23.1%), followed by lymphnodes, bones, and liver. The clinical examinations performed and diagnostic techniques used were, renal function (BUN, Serum Cr), Hb, WBC, liver function (T. Bil, GOT, GPT), serum protein fraction, serum LDH, serum Ca, ESR, tumor marker (AFP, CEA), urine cytological examination, blood pressure, IVP (or RP), angiography. As the therapeutic method, nephrectomy was performed in 25 cases (35.2%), combined nephrectomy and irradiation therapy in 12 cases (16.9%), combined nephrectomy and chemotherapy in 11 cases (15.5%), combined nephrectomy and other therapy in 15 cases (21.1%), and conservative therapy in 8 cases (11.3%). For the entire traced series of renal tumors, the 1-, 3-and 5-year survival rates were 72.3, 49.8, and 49.8% respectively. For renal parenchymal tumors (renal adenocarcinoma), the 1-, 3-and 5-year survival rates were 77.8, 53.0, and 53.0%. The most important factor of prognosis was the stage of tumor. Patients with elevated erythrocyte sedimentation rate, and dysproteinemia also had distinctly unfavorable prognosis. In this study of therapy, the highest survival rate was seen for the patients treated by combined nephrectomy and irradiation therapy of both renal parenchymal and pelvic tumors.
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HLA antigens were determined for the A, B and C loci among 86 Aetas of west-central Luzon and 87 Mamanwas of north-eastern Mindanao, Philippines. The overall antigenic profiles of these two groups were essentially similar to those of other Asian-Pacific populations with some peculiarities in common with Papua New Guineans. However, the results of heterogeneity tests and the comparison of statistically significant phenotypic associations showed a highly significant difference between the Aeta and the Mamanwa groups. It was postulated that the two "Negrito" groups represent different migrations to the Philippines.
Eighty-eight Japanese patients with rheumatoid arthritis and 104 normal Japanese persons were typed for HLA A, B, C, and DR antigens. The frequency of HLA DR4 was 70.5% in patients compared with 46.1% in normal controls (P less than 0.001). However, a sex difference in the frequency of HLA DR4 in patients was noted. HLA DR4 was found in 80.6% of male patients, which was highly significant compared with controls (P less than 0.0005), while only a borderline increase of 60.5% was found in female patients (P less than 0.05). In addition, the frequency of HLA DR2 was remarkably low in male patients. These suggest the possible heterogeneity of rheumatoid arthritis in Japanese.
We have described clinical features of 53 cases with pustulotic arthro-osteitis. Anterior chest wall symptoms such as intersterno-costoclavicular or manubriosternal lesions were observed in all of 53 cases. Spondylitis or spondylodiscitis was found in 18 cases. Sacroiliitis resembling ankylosing spondylitis was seen in 7 cases. Peripheral inflammatory arthritis was seen in 14 cases, which were of nonerosive, of oligoarthritis type, and cured within 1 to 2 months, leaving no residue. HLA B27 was never found, and RAW factor was negative. Histological examinations revealed nonspecific chronic inflammation of bone and soft tissue. Pustulotic arthro-osteitis is apparently distinct from known rheumatic diseases such as rheumatoid arthritis, ankylosing spondylitis, psoriatic arthritis, and Reiter's disease. We have proposed that this condition should be classified as a member of the "seronegative spondylo-arthritis' group as designated by Wright and Moll.
The protease-resistant character of malignantly transformed cells was studied. Surface labeling experiments revealed that the cell surface of transformed cells treated with, or grown in the presence of a neutral protease (Dispase) was drastically altered by proteolytic digestion, and showed that transformed cells could be stripped of many of their cell surface components without losing cell viability and growth ability. The protease-resistant character depended upon the degree of cell-crowding and was not expressed unless transformed cells were seeded at a high cell density, suggesting that some conditioning factor(s) contributes to its expression. The protease-resistant cells (K-N7-8) could proliferate in serum-free, chemically defined medium and secreted growth-promoting factor(s) that allowed serum-free propagation of 3T3 cells (protease-sensitive cells). The test for protease resistance was presumed to discriminate between growth factor-secreting and non-secreting transformed cells. The ability of transformed cells to produce the growth factors that they need for continuous multiplication may be one of the mechanisms by which malignant cells escape from host growth control and become able to grow autonomously.
A clonal cell line of murine fibroblast (ST 13) undergoes a terminal differentiation into adipose cells in culture. A tumor promoter, 12-O-tetradecanoyl phorbol 13-acetate (TPA), reversibly inhibited the differentiation of ST 13 preadipocytes. The inhibition of differentiation was accompanied by changes in cell morphology and growth properties and by suppression of cellular insulin binding activity. Dexamethasone (DXM) could prevent the inhibition by TPA of preadipocyte differentiation, concomitant with prevention of the morphological changes and an increase in insulin binding activity. TPA-induced plasminogen activator (PA) secretion and DXM-mediated inhibition of PA activity could not be correlated with inhibition/prevention-of-inhibition of differentiation.