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Biomedical subjects

H Meyer

Publications and source records attributed to H Meyer.

At least 523 records · Page 29Linked to original sources
PubMed1976

The influence of cholic acid and dehydrocholic acid on the biliary excretion of unconjugated and conjugated sulfobromophthalein in rats.

Urethane anesthetized Wistar rats with biliary fistulas were infused during 100 min with sulfobromophthalein (BSP), the glutathione conjugate of sulfobromophthalein (BSP-GSH), cholic acid (CA) and dehydrocholic acid (DCA). The dyes (594 nmol/100 g/min) and the bile acids (1200 nmol/100 g/min) were infused separately, and in combination as well. When BSP was infused, CA and DCA increased the maximal excretion of total BSP (conjugated plus unconjugated) from 1400 to 4100 and 3300 nmol/100 g/10 min. The bile flow observed with BSP plus CA was not significantly different from that with BSP plus DCA. The biliary excretion of total BSP was higher throughout with CA than with DCA because CA increased the biliary concentration of PSP while DCA did not. The bile flow attained with CA alone was significantly lower than that with BSP plus CA. The current data provide arguments for abandoning the view that choleresis per se is the crucial determinant for BSP excretion. When BSP-GSH was infused instead of BSP, the excretion rate of the dye was not altered by the additional infusion of CA whereas it was significantly reduced by DCA. The maximal biliary concentration of BSP-GSH fell from 25.9 nmol/mul to 15.3 and 9.4 nmol/mul with CA and DCA, respectively. Both CA and DCA impaired the hepatic uptake of BSP and BSP-GSH. During the infusion with CA, BSP plus CA and BSP-GSH plus CA the biliary excretion rates of bile acids did not differ significantly from each other. This favours the view that the transfer for CA from the liver to bile is different from that for BSP and BSP-GSH. A fraction of bile fluid "independent of choleretics" (viz. of bile salts, BSP and BSP-GSH) is estimated and discussed in view of the different types of infusion.

Animals↗
PubMed1976

[Periodontal reaction to interdental osteotomy].

Interdental osteotomy presents a small risk for the tooth adjacent to the line of the osteotomy. Five out of 41 teeth adjacent to the site of an osteotomy showed loosening degree 1. The PM index rose from 1.0 to 1.4. The depth of the periodontal pockets did not change. The roots of two out of 41 teeth were damaged during osteotomy, but not devitalized.

Alveoloplasty↗
PubMed1976

[General adaptation syndrome (Selye) in cattle. 6. Influence of stress conditions on antibody levels after active and passive immunization as well as on the topographic distribution of various groups of pathogens in the gastrointestinal canal].

The action of experimental and natural stressors on the humoral immune response of calf was studied. Particular attention was given to the H-antigen of S.dublin and equine erythrocytes,the degradation rate of passively acquired humoral antibody, as well as the quantity and topographic distribution of certain groups of germs in the gastrointestinal tract. The following results were obtained:1. Antibody formation was impededby repeated or lasting stressor effect (ACTH injections). 2. The immunological reactions of the calves involved to antigen injection immediately after transport into the rearing unit were stronger than those to antigen application three days from transfer. Immunisation of animals transferred to rearing or fattening units, therefore, should be applied immediately after arrival in the new accomodation, but no interval of three or four days should be allowed. 3. Antibody formation was no longer impaired in calves immunised two weeks from transfer, as compared to those immunised immediately after arrival in the rearing unit. This seemed to suggest that by that time adaptation of the animals to their new environment had been almost complete. 4. Lasting stress (slow drip infusion of ACTH, cortisol, colibacteria or coli-endotoxin) led to no detectable by paper electrophoresis. 5. Calves that had been given three weekly dosesof 1 IU ACTH per kilogram of live weight through four weeks,did not differ,withthe authors'method,from the controls regarding the decomposition rate of passively acquired humoral antibody. 6. ACTH slow drip infusions of calves over several days caused higher concentrations of colibacteria throughout the intestinal tract, including those proximal sections of the small intestine in which little or no colibacteria should occur under physiological conditions in calves of that age.

Adaptation, Physiological↗
PubMed1976

[Culture media for Salmonella dublin on a chemically defined basis].

The nutrient demand of S-dublin strains was investigated. Media on chemically defined basis for culturing of S. dublin are recommended with reference to the above studies. The definition includes Na2HPO4, KH2PO4, NaCl, glucose, the amino acids L-asparaginic acid, L-cystine, L-glutamic acid, serine, and L-threonine, and nicotinamide as vitamin component. Such media will be quite favourable for certain culturing problems, but complex substrate (yeast extract, peptone, tryptone) should be added for high yields.

Culture Media↗
PubMed1975

Hypertrophic non-obstructive cardiomyopathy caused by disorder of the myofiber texture.

A case of hypertrophic non-obstructive cardiomyopathy caused by a disorder of the myofiber texture was observed in a 10 year old boy. The heart weighed 390 g and showed concentric hypertrophy of the left and right ventricles as well as of the ventricular septum. Additional findings consisted in an abnormal septum membranaceum and a accessory tricuspid valve leaflet. Severe cardiac hypertrophy was associated with prominent interstitial and subendocardial fibrosis, and pronounced intimal fibrosis of the intramural arteries. Electron microscopy revealed various degrees and stages of hypertrophy of the myocardial cells combined with severe degenerative changes. Additional changes of the sinus node and conduction system were responsible for a tachycardia-bradycardia syndrome, complete left bundle branch block and final total AV-block.

Autopsy↗
PubMed1975

An electron microscopic and cytochemical study of the cell coat of Trypanosoma cruzi in tissue cultures.

Using the cytochemical method of Thiery, a polysaccharide surface coat was demonstrated with the electron microscope in the various developmental stages of Trypanosoma cruzi maintained in tissue cultures. The cell coat was observed on the whole surface membrane system of T. cruzi. A positive reaction, similar to that observed in the cell membrane, was also obtained in the membranes of some vacuoles in the cytoplasm of the parasites. No granules were found in the parasites. No granules were found in the parasite which could represent reserve polysaccharides. The possible role of the cell coat in such phenomena as adhesion, agglutination, phago- and pinocytosis and antigenicity is discussed.

Adhesiveness↗
PubMed1975

[Collection of hematopoietic stem cells from canine peripheral blood and their ability to regenerate hematopoiesis].

A procedure is presented for the collection of a large number of hemopoietic stem cells from the peripheral blood of dogs by means of a single leukapheresis using the NCI-IBM Blood Cell Separator. In the course of a leukapheresis of about 285 min duration a mean of 23 x 10-9 leukocytes is collected from the blood. The hemopoietic stem cells among such separated leukocytes initiate repopulation of bone marrow within 10 days after whole body X-irradiation with 1200 R. The cell numbers in a defined histological section of femoral bone marrow are evaluated 9 to 10 days after irradiation and subsequent autologous transfusion of 6.72 x 10-9 separated mononuclear leukocytes. The results indicate that the bone marrow cell numbers of transfused dogs are significantly greater than in dogs given only 1200 R and reach a level of approximately 49% of the normal value. Possible ways of increasing the yield of hemopoietic stem cells from the peripheral blood will be considered.

Animals↗