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Biomedical subjects

H Metz

Publications and source records attributed to H Metz.

At least 55 records · Page 3Linked to original sources
PubMed1975

Serum concentrations of ampicillin and probenecid and ampicillin excretion after repeated oral administration of a pivampicillin-probenecid salt (MK-356).

Twenty male volunteers received oral doses (2100, 1050, and 525 mg) of a pivampicillin-probenecid salt in a 1 to 1 molar ratio (MK-356) at 12 hour intervals. After each dose peak serum concentrations of probenecid were observed 2 hours later than peak concentrations of ampicillin. Following the first dose of MK-356 the apparent elimination rate of ampicillin was dose-dependent and did not follow first order kinetics, as it showed a longer apparent half life after a higher dose. An equal dose of MK-356 administered 12 hours later caused an increase in the peak serum ampicillin level greater than expected from the concentration of ampicillin after the preceding dose. In twelve male volunteers who received at random 525 mg of MK-356 or 350 mg of pivampicillin, each three times daily for 4 days, the areas under the ampicillin concentration curve were the same after the first or last dose of either drug. When 2100 or 1050 mg of MK-356 was taken as an initial dose, 30 to 40 per cent of the ampicillin was recovered from urine in the ensuing 12 hours. The results indicate that when at least 400 mg probenecid was coadministered twice daily with 700 mg pivampicillin (MK-356), the peak serum concentrations of ampicillin were increased and its elimination rate slowed following successive doses.

Administration, Oral↗
PubMed1975

[Bactericidal activity of serum and chemotherapy in sensitive and resistant exciter (author's transl)].

Comparing examinations with Ampicillin sensitive and resistant bacteria-strains show that the bactericidal activity of serum is dependent on the bacteria-strains, on the Ampicillin sensitivity of the particular exciter and on the number of bacteria/ml (germ count). Bactericide effect could always be obtained with sensitive strains as a result of additional chemotherapy. With several resistant strains a bactericide effect could not be obtained in this case the continuous optimal Ampicillin addition was the decisive factor. Because of the extremely complicated process of the bactericide one should not make general conclusions from the individual experimental results.

Ampicillin↗
PubMed1975

[Symbiosis and antibiosis of mycoplasma and bacteria (author's transl)].

The typical colony formations of mycoplasma are strongly influenced by reduced amounts of serum in the agar. The interactions between different types of mycoplasma and bacteria were investigated on antibiotic-free mycoplasma agar. The behavior of the strains of bacteria tested shows distinct differences against M. hominis in comparison with M. fermentans, M. arthritidis and Acholaplasma Pg 8.

Bacteriological Techniques↗
PubMed1975

[Toxin production by enteropathogenic colibacilli in adult persons (author's transl)].

Culture filtrates of enteropathogenic strains of E. coli from adult patients with cholera-like diarrhoea produced a rhythm-disturbing effect when injected into isolated sacklets of rabbit ileum. This action was shown to be medium-dependant. It could not be elicited by cultures grown in synthetic medium. Meat extract cultures gave variable results, but the gut movements could be irritated regularly when cultures were grown in a casamino acids - yeast extract medium (table, figure). This activity which was not associated with non-pathogenic strains appeared in the beginning of the stationary phase of growth. The factor responsible for the dysrhythmic effect could be precipitated by ammonium sulphate, was dialysable, withstood boiling for 10 minutes (figure) and treatment with trypsin, chymotrypsin and pancreatin. Antisera prepared against culture filtrates of strain 10407 containing agglutinating and vascular permeability neutralizing antibodies did not neutralize the gut irritating effect efficiently. We conclude that the factor of our assay system is perhaps closely related to the heat-stable enterotoxin described by other authors concerned by E. coli enterotoxins.

Adult↗
PubMed1975

Staphylococcal micrococcins. II. Isolation, purification and identification.

Seven strains belonging to the Micrococcaceae family and excreting substances with antibiotic activity, were grown in submerged cultures on technical scale for isolation, purification and identification of biologically active compounds. Two basic substances were isolated and classified to the micrococcin antibiotics family. The naturally occurring mixture of micrococcin M1 and M3 was called micrococcin M. This antibiotic has the formula C48H50O11N12S6 and a molecular weight of about 1160, melting point 221--224 degrees C, and optical rotation [a]20/D = + 66.6. Other antibiotically active substances produced by seven investigated strains were identified as micrococcin M or as separate compounds. Comparison with previously described micrococcin and micrococcin P has been made.

Anti-Bacterial Agents↗
PubMed1975

[Bacteriological studies on the therapy of bacillus pyocyaneus infections].

The effect of combined antibiotics was studied in 25 strains of Pseudomonas aeruginosa by serial diluting tests. A synergistic effect was stated in 15 strains with oxacillin plus ampicillin, and in 7 strains with carbenicillin plus ampicillin. In these examinations, the quantities of antibiotics needed each time were within the highest admissible level of therapuetic concentrations. A constant rate of the optimum single concentrations could not be observed. On principle, the inhibitory tobramycin concentrations (0.06 - 1 gamma/ml) were lower than the corresponding concentrations of gentamycin (0.25 - 2gamma/ml); tobramycin was more effective than gentamycin also in 6 strains resistant to carbenicillin, tobramycin only had a synergistic effect - in five of these six strains - when very high ampicillin concentrations (i.e. 250-500 gamma/ml) were used.

Ampicillin↗
PubMed1975

[A glucose dehydrogenase for the determination of glucose concentrations in body fluids (author's transl)].

The isolation of glucose dehydrogenase from Bacillus megaterium M 1286 is outlined. Data on the specificity of the enzyme towards carbohydrates are given. A specific method for glucose determination using this enzyme was developed. Methods and results of four variants of this glucose determination are presented: End point determination in the UV range, determination with formazan as reaction product, kinetic determination in the UV range, and continuous flow analysis in the UV range (AutoAnalyzer method).

Alcohol Oxidoreductases↗