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Biomedical subjects

H Matsui

Publications and source records attributed to H Matsui.

At least 613 records · Page 34Linked to original sources
PubMed1990

Stability of cerebral blood flow and oxygen metabolism during normal aging.

Cerebral blood flow and oxygen metabolism in aged normal subjects were measured with positron emission tomography and their relationship with brain atrophy was evaluated. Brain atrophy progressed in a linear fashion during aging while cerebral blood flow and oxygen metabolism were well preserved. The finding suggests that brain atrophy is an aging process less dependent on cerebral blood flow and metabolism over the course of normal aging.

Aged↗
PubMed1990

The immunotoxicity of triphenyltin chloride in mice.

The immunotoxicity of triphenyltin chloride (TPTC) in mice was investigated. 1) When TPTC was injected intraperitoneally (i.p.) into mice at doses between 1 and 10 mg/kg for 14 d, a reduction in the weights of thymus and spleen was noticed, however, the body weight was not changed significantly. 2) The production of hemolytic plaque forming cells in the spleen of mice immunized with sheep red blood cells (SRBC) was inhibited by the administration of 10 mg/kg of TPTC. 3) The i.p. injection of TPTC at a dose of 10 mg/kg for 5 d after the primary immunization of mice with dinitrophenylated ascaris antigen resulted in suppression of immunoglobulin E antibody formation in primary immune response, but did not affect the secondary immune response. 4) The production of antibody against polyvinylpyrrolidone (T cell independent antigen) was not affected by the administration of TPTC. 5) The production of effector T cells which are able to cause SRBC- or tuberculin-induced footpad reaction in mice and the induction of cytotoxic T cell in local graft versus host reaction in mice were also inhibited by TPTC. These results indicate that TPTC inhibits both the T cell dependent humoral and cellular immune responses in mice.

Animals↗
PubMed1990

In vivo analysis of extracellular proteins in rat brains with a newly developed intracerebral microdialysis probe.

Peptides and proteins in the extracellular space in the central nervous system were investigated in vivo using an intracerebral microdialysis probe. The molecular cut-off of the hollow fiber which was used for the probe was approximately 100 kDa. We examined recovery rates of several compounds in vitro. The recovery rates of proteins and peptides were between 7-28%, with the exceptions of substance P and insulin-like growth factor I. The recovery rates of monoamines and their metabolites were 22-40%. In in vivo studies, two major proteins with apparent molecular weights of 62 kDa and 12 kDa, and several minor proteins (28 kDa, 43 kDa, 52 kDa and 70 kDa) were detected by SDS-polyacrylamide gel electrophoresis in the dialysate from a probe implanted in the striatum of anesthetized rats. These results suggest that the newly developed, intracerebral microdialysis probe might be useful for investigating the dynamic changes of peptides and proteins in the central nervous system.

Animals↗
PubMed1990

Hyper-heterogeneity of the N-terminus of recombinant BSF-2/IL-6 produced in CHO and NIH3T3 cells.

Recombinant human BSF-2 (B cell stimulatory factor 2/Interleukin 6; IL-6) proteins were purified from CHO and NIH3T3 cell cultures and characterized. The lectin binding patterns suggested that the proteins have N-linked oligosaccharide(s) with tri-antennary structure of bisecting GlcNAc. Their N-termini were highly heterogeneous; at least five closely related N-termini were detected. This N-terminal heterogeneity was not generated in the cell culture because no processing activity was found in the culture medium.

Amino Acid Sequence↗
PubMed1990

[Comparisons of effects of vitamin D3 metabolites on mineralization: utilization of incisor dentin in parathyroidectomized rats].

The rat incisor is continuously growing throughout the animal's life recording the state of mineralization within its dentin at all times. Therefore, it was assumed that the incisor dentin was suitable material for studying the effects of various drugs on such mineralization. The present study set out to examine the effects of vitamin D3 metabolites on the remineralization of dentin and the serum calcium level in parathyroidectomized (PTX) rats. Male Wistar rats weighing about 200-250 g were parathyroidectomized. Each rat was given daily 12 g of a synthetic diet containing 0.1% Ca and 0.4% P (normal P) or 0.06% P (P deficient) without vitamin D. Exp. I: Three groups of rats given a normal P diet were orally administered vehicle, 25 (OH) D3 (50 IU/rat) or 1,25 (OH)2D3 (5 IU/rat) for 5 days from the 28th day after PTX, respectively. Exp. II: Two groups of rats given a normal P diet or a P deficient diet were further divided into 4 sub-groups and intramuscularly administered 0, 5,000, 10,000 or 20,000 IU of vitamin D3 on the 9th day after PTX, respectively. The active vitamin D3, 1,25 (OH)2D3 restored the mineralization of the dentin which had been suppressed following PTX, and elevated the serum calcium level to about 9 mg/dl. However, the 25 (OH) D3 neither restored the mineralization of the dentin nor elevated the serum calcium level in the PTX rats. At doses below 1,000 IU, vitamin D3 could not restore the mineralization of the dentin. However, at massive doses above 5,000 IU, the mineralization was restored in proportion to the dose. The serum calcium levels also increased in proportion to the dose. It is assumed that the increase of the serum calcium level was mainly due to the enhancement of calcium absorption from the intestine by vitamin D3. Both remineralization of the incisor dentin and increase of the serum calcium level were more marked in groups of rats given a P deficient diet than in those given a normal P diet. In all cases in the present experiments, the degree of mineralization of the dentin seemed to correspond with the degree of elevation of the serum calcium levels. These results suggest that the remineralization of the dentin in the PTX rats was attributable to the effect of the vitamin D3 metabolites on serum calcium regulation. Relative biological activities of 1,25 (OH)2D3, 25 (OH) D3 and vitamin D3 on the remineralization of dentin were estimated to be 1, less than 0.02 and less than 0.001, respectively.

Animals↗
PubMed1990

Characteristics of cerebral glucose utilization in dementia.

To make clear the characteristics of cerebral glucose uterization in dementia, PET studies with 18F-FDG were carried out. Taking the pattern of 18F-FDG uterization, dementia can be subdivided into two types. One type shows a simultaneous and symmetrical reduction glucose uterization in the posterior part of neocortex covering the temporal, parietal and occipital association cortices. This is referred to as type I. Although this type constitutes only about 1/5 of all dementia patients, it is considered the fundamental type of dementia. Aside from this, there is type wherein a simultaneous and symmetrical reduction in glucose uterization of the neocortex. This is type II. It constitutes about 4/5 of all dementia patients which is far more type I. There are no essential difference in the characteristics of cerebral glucose uterization in AD and MID. However, with regards the mean, AD is lower than MID. Various organic defect in neocortex do not correlate with the global reduction in glucose uterization in dementia patients. These results suggest that the reduction in glucose uterization in dementia may be functional disorder.

Aged↗
PubMed1990

Severe atrophy of amygdala and hippocampus in both Alzheimer's disease and multi-infarct dementia.

Alzheimer's disease and multi-infarct dementia are the two main diseases entirely different from each other producing almost the same abnormalities as dementia syndrome. With magnetic resonance imaging, severe atrophy of amygdala and hippocampus in both Alzheimer's disease and multi-infarct dementia was observed. There was no essential difference on the atrophic findings between Alzheimer's disease and multi-infarct dementia. This result therefore suggest that the quantitative measurement of amygdaloid and hippocampal atrophy predicts dementia.

Aged↗
PubMed1990

Ceramic interspinous block (CISB) assisted anterior interbody fusion.

Ceramic interspinous block (CISB) assisted anterior fusion system was developed. The system is recommended for patients with symptomatic disc degeneration and destructive lesions of the lumbar spine. One hundred and six patients who underwent CISB-assisted anterior fusion by one doctor (H.T.) from 1981 to 1987 were followed-up 2-6 years after the operation. A comparative study of the results between the CISB-assisted group and the CISB-unassisted anterior fusion group, performed by the same doctor during 1979-1981 for deranged disc, was also done. Satisfactory relief of back and leg pains, marked ADL recovery and early bone union were obtained in all of the CISB-assisted anterior fusion cases. The union rate was 98.9% in the CISB-assisted anterior fusion group, whereas in the CISB-unassisted group, it averaged 79%. The efficacy of CISB for anterior interbody fusion was also verified using a computer simulation. CISB implementation appears to create equalization of stress distribution on the graft-vertebra interface.

Adolescent↗
PubMed1990

Fibronectin on prosthetic denture fabrication.

It is well known that fibronectin adheres to various oral bacteria and shows affinity to various components. Thus the existence fibronectin which is present in the salivary flow, acquired pellicle and dental plaque, was investigated in used acrylic resin dentures with a direct immunohistochemical fluorescent technique using antihuman fibronectin goat serum which was labeled with fluorescein isothiocyanate (FITC). It was found that fibronectin frequently was present on the surface of acrylic resin dentures.

Acrylic Resins↗
PubMed1990

[Effects of 1,25-dihydroxyvitamin D3 on mineralization of incisor dentin in parathyroidectomized rats].

Male Wistar rats (8 weeks old) were parathyroidectomized (PTX) and given daily subcutaneous injections of different doses (0, 25, 50 or 100 ng/kg) of 1,25 (OH)2D3 for 13 days. Changes in plasma calcium and phosphorus levels were monitored at 3 day intervals. An appositional rate of dentin was estimated by a time marking method; that is, the distance of two lead lines deposited in a transverse section of the incisor dentin on the 9th and 12th days of the experimental period was measured. The plasma calcium levels that had been lowered by PTX were elevated dose-dependently following injections of the drug, but the plasma phosphorus levels that had been elevated by PTX were lowered. The body weight was not affected by the administration of the drug. However, the values for the dry weight and ash weight, and the calcium and phosphorus contents of the upper incisors increased dose-dependently. The mineralization of the dentin, that had been suppressed by PTX, also recovered dose-dependently. Apposition rates of the dentin in groups of rats given various doses of the drug (25, 50 and 100 ng/kg/day) averaged 46, 61 and 69 microns/3 days respectively. There was a significant correlation between the apposition rate of the dentin and the plasma calcium level. The correlation coefficients were estimated to be 0.956 (y = 17.2 + 4.6 chi, p less than 0.001) on the lingual aspect and 0.941 (y = 11.4 + 5.3 chi, p less than 0.001) on the labial aspect. However, the values for the mineralization of incisor dentin were inversely proportional to the plasma phosphorus levels. These results indicate that the stimulating effect of 1,25 (OH)2D3 on the mineralization of the dentin in PTX rats is primarily dependent upon the increase in plasma calcium levels by the hormone. The direct effect of 1,25 (OH)2D3 on the mineralization of dentin could not be demonstrated in this experiment.

Animals↗
PubMed1990

[A clinicopathological study on gestational trophoblastic disease comparing with high risk criteria and diagnostic score of choriocarcinoma].

In the past 15 years, 274 patients with the gestational trophoblastic disease have been treated at Chiba University and the diagnosis of 125 patients was confirmed pathologically. There were 55 cases (44%) in the high risk and 70 cases (56%) in the low risk group with the following criteria: (1) the urinary hCG titer was more than 100,000 IU/L; (2) the duration between antecedent pregnancy and the start of treatment was more than 1 year; (3) antecedent pregnancies were full-term or abnormal pregnancies except hydatidiform mole. These pathologically confirmed patients were evaluated with the choriocarcinoma diagnostic score. From this study, the following results were obtained: (1) The overall remission rates in high risk and low risk patients were 79.1%, 100%, respectively. (2) Out of 55 patients with high risk diagnosed pathologically, 49 (89.1%) patients had choriocarcinomas. On the other hand, 65 (92.9%) patients with low risk had invasive moles. (3) The discrepancies between the pathological and the clinical diagnosis chiefly depended on the inadequate evaluation of recurrent cases and the pretreatment urinary hCG. (4) Compared with our criteria, the choriocarcinoma diagnostic scoring system more closely corresponds to the pathological diagnosis but this system is more complex and inadequate than our criteria for older up patients that have not been adequately followed up.

Biomarkers, Tumor↗
PubMed1990

Purification and immunohistochemical study of actin in mitochondrial matrix.

Actin was purified to apparent homogeneity from the matrix of ultra-pure mitochondria of rat livers by DNase-I affinity chromatography and HPLC gel filtration. The mitochondrial actin was immunologically identified by an anti-actin antibody, and its apparent molecular weight was 43 KDa, as determined by SDS-polyacrylamide gel electrophoresis. The immunohistochemical study revealed the localization of the mitochondrial actin in the matrix space and on the internal surface of inner membrane. The actin fraction eluted from a DNase-I column by KCl-EGTA solution underwent polymerization and bundling in vitro.

Actins↗
PubMed1990

[In vitro synergism of two-drug combinations among cefamandole, flomoxef and imipenem against MRSA].

First of all, vancomycin (VCM) showed good antimicrobial activity against Staphylococcus aureus including methicillin-sensitive and -resistant strains. MIC90 of VCM was 1.56 micrograms/ml. Effects of different 2-drug combinations among cefamandole (CMD), flomoxef (FMOX) and imipenem (IPM), and activity of VCM alone were examined in vitro against 5 clinical isolates of methicillin-sensitive S. aureus (MSSA) and 22 isolates of methicillin-resistant S. aureus (MRSA). In a checkerboard study, synergism was observed against 4 strains of the 5 MSSA and all 22 strains of the MRSA with IPM and CMD, FMOX and IPM, and FMOX and CMD combinations. Mean MICs of these combinations were as low as one-fourth to one-tenth as those of individual drugs.

Cefmetazole↗
PubMed1990

[Intraperitoneal administration of anti-cancer drug with cisplatinum (CDDP)].

Six patients were administered cisplatinum (CDDP) i.p. There were four cases with peritoneal dissemination of gastric cancer after gastrectomy and the other two, for whom prophylactic intraperitoneal administration of CDDP had been attempted to prevent peritoneal metastasis. The dose of CDDP was 75-140 mg/body (58-90 mg/m2). The results were 1 PR, 2 NC and 1 PD. The PR case was given intraperitoneal CDDP combined with RF thermotherapy. Liver dysfunctions, renal dysfunctions, bone marrow suppressions, and GI tract disturbances were observed as toxic reactions. The degrees of side effects after i.p. administration were less severe than after i.v. administration. However the indication of CDDP i.p. administration should be decided after case conferences. On pharmacokinetics, non-protein bound platinum was detected at 24 hours after i.p. administration, in ascites and plasma. The concentration of platinum in ascites was about three-fold than in plasma. It was considered that CDDP absorption from ascites into plasma depended on the state of peritoneal dissemination of cancer.

Adult↗
PubMed1990

[The treatment of refractory choriocarcinoma].

The choriocarcinoma was one of the most curable malignant neoplasms. This is chiefly because the effective chemotherapy was introduced and the sensitivity of hCG was improved. But about 10-20% cases with choriocarcinoma were drug resistant or recurrent cases. And now, the treatment of these refractory choriocarcinomas were one of the most important clinical problems. We describe a new combination chemotherapy for these refractory choriocarcinomas in this paper. This high dose 5-FU + Act-D protocol was effective for drug resistant cases with MEA protocol.

Adult↗