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Biomedical subjects

H Maeda

Publications and source records attributed to H Maeda.

At least 937 records · Page 52Linked to original sources
PubMed1989

[Clinical study of aminoglycosides on renal dysfunction--pharmacokinetics of arbekacin and its elimination effects by hemodialysis and adsorption with charcoal].

In vitro studies of elimination of arbekacin (HBK), a new aminoglycoside antibiotic, from blood by means of hemodialysis or adsorption with charcoal, and pharmacokinetic studies in patients with renal dysfunction were examined. HBK was well eliminated by hollow fiber type artificial kidney (HFAK) with a half-life of 0.13 hr. HBK was also well eliminated by an adsorption tube containing charcoal with even shorter half-life of 0.03 hr. As to pharmacokinetics of HBK in patients with renal dysfunction, blood levels became higher with a greater reduction in 24 hr endogenous creatinine clearance (Ccr). A decreasing tendency was also seen in urinary recovery rate. These results indicated that hemodialysis and adsorption with charcoal are useful for elimination of HBK from blood. It is recommendable in patients with renal dysfunction to take some measures such as prolongation of administration interval of HBK according to the extent of decrease in Ccr.

Adsorption↗
PubMed1989

[The result of extended thymectomy in patients with myasthenia gravis of pure ocular type].

We studied postoperative status of 14 patients with myasthenia gravis of ocular type who underwent extended thymectomy. Nine patients were in remission, three improved, and two unchanged. No patient became worse and died. The remission rates at one, three, five, and ten years after operation were 50.0%, 58.3%, 60.0%, and 80.0%. The palliation rates at one, three, five, and ten years after operation were 64.3%, 75.0%, 80.0%, and 100%. The remission rate at one year after operation in patients of ocular type was significantly (p less than 0.05) higher than that in generalized type (191 patients). The mean preoperative duration of symptoms in patients who obtained remission after surgery was 7.2 +/- 6.5 months, while mean duration was 85.6 +/- 45.8 months in those patients who could not obtain remission, indicating a significant difference (p less than 0.05) of duration of symptoms between two groups. Among 89 patients with generalized as well as ocular symptoms before extended thymectomy, 62 patients (69.7%) still complained of ocular symptoms and 48 patients (53.9%) had generalized symptoms with or without ocular symptoms in 1 to 12 years after operation. This result shows that ocular symptoms do not disappear more easily than generalized ones. We conclude that extended thymectomy should be performed even in patients with myasthenia gravis of pure ocular type.

Adult↗
PubMed1989

[Surgical treatment of pulmonary metastases from osteogenic sarcoma-significance of aggressive resection of bilateral multiple metastases and tumors invading adjacent organs].

The results of surgical treatment of pulmonary metastases from osteogenic sarcoma were analyzed. Total cases were 23 with mean age of 17.0 +/- 9.1. The number of operations were 46, and operative procedures consisted of pneumonectomy in 1, lobectomy in 3, partial resection in 37 and resection of adjacent organs in 5. The overall five-year survival rate from the first thoracotomy was 21%. This was significantly higher than prognosis of the previous cases with pulmonary metastases which were not resected (p less than 0.001). Three-year survival rate for the group with 5 or less metastases was 57%, whereas that for the group with 6 or more metastases was 0% (p less than 0.02). To assess both the number and the diameter of metastases, an index called number-size score (NS score) was introduced, which was the sum of the diameters of each metastatic lesion. The three-year survival rate for the group with NS score 6 or less was 73%, whereas that for the group with NS score 7 or more was 9% (p less than 0.001). Disease free interval, the time of operation and histological effect of chemotherapy were not significantly related with prognosis. There was no long-term survivor among the patients with resection of adjacent organs. In conclusion, an aggressive resection of pulmonary metastases from osteogenic sarcoma should be performed with intensive chemotherapy, but significance of the resection of multiple metastases more than 20 or tumors invading adjacent organs is doubtful.

Adolescent↗
PubMed1989

[Study on indication of unilateral pulmonary artery occlusion test in patients undergoing pneumonectomy].

We tried to establish an indication of unilateral pulmonary artery occlusion test (UPAO), by analyzing the preoperative data of UPAO, overall and regional pulmonary function tests, and arterial blood gas in 55 patients who were intended for pneumonectomy. Our limits of functional indication for pneumonectomy were PPA less than or equal to 25 mmHg or TPVRI less than or equal to 700 dyne.cm-5.m2. PPA correlated with the inverse of contralateral FEV1.0/BSA (FEV section 1.0) and DLCO/BSA (section DLCO), which were calculated by multiplying preoperative values by contralateral ventilation fraction. TPVRI correlated with age, the inverse of PaO2, the inverse of FEV section 1.0, and the inverse of section DLCO. Multiple regression analysis yielded the equation, TPVRI approximately p = 1120/section DLCO + 24800/PaO2 + 6.40 X Age -506. In order to make false negative zero, the values of each parameter were as follows: age greater than or equal to 55, PaO2 less than or equal to 85 mmHg, FEV section 1.0 less than or equal to 0.8 L/m2, section DLCO less than or equal to 6.0 ml/min/mmHg/m2 and TPVRI approximately p greater than or equal to 450 dyne.sec.cm-5.m2. The specificities of each parameter were 18.6%, 20.9%, 39.2%, 40.4% and 59.0%. Therefore, in order to make false positive patients least, the criteria of TPVRI approximately p greater than or equal to 450 dyne.sec.cm-5.m2 should be used.

Adult↗
PubMed1989

[Assessment of the left atrial myxoma with gated blood pool SPECT].

A left atrial myxoma with 4 cm diameter was studied with ECG gated Tc-99m blood pool SPECT. The long axial images showed the myxoma to protrude through the mitral valve (MV) during diastole and to come back into the atrium in systole. No significant atrial dilatation due to the MV obstruction was detected. The ejection fraction calculated from the SPECT images was 10% lower than that of the conventional method viewed from LAO 45 degrees. Nuclear medicine gave an additional information for the assessment of the hemodynamics.

Aged↗
PubMed1989

Ultrastructural changes in human erythrocytes caused by heat and the localization of ABO blood group activity.

The ultrastructural changes in human erythrocytes caused by heat and the localization of ABO blood group activity have been studied electron microscopically. Osmotically hemolyzed cells were found to show varying degrees of cell matrix loss and a swelling of the membranes. By means of immunoferritin staining, ferritin particles, which indicate the localization of ABO group activity, were found to bind in clusters on small process-like structures on the membranes. Erythrocytes heated to 60 degrees C showed a hemolytic change; the cell matrix was released and small globular substances (ca. 50-100 nm in diameter) aggregated on the cytoplasmic side of the partially swollen cell membranes, a phenomenon that was not noted in erythrocytes that undergo a hypo-osmotic change. Erythrocytes heated to 100 degrees C underwent fragmentation, though the cell matrix was almost preserved and aggregated substances of varying size were noted inside the membranes. ABO group activity was demonstrated almost evenly on the sub-membrane aggregated substances and on the surface of the thermo-changed erythrocytes.

ABO Blood-Group System↗
PubMed1989

[A pleomorphic lipoma of the palm--comparison to spindle cell lipoma].

A pleomorphic lipoma usually occurs in the neck, the shoulder, and/or on the backs of men over the age of 50. This tumor displays a mixture of fat cells, pleomorphic cells, floret cells, and bundles of mature collagen fiber. Herein we report on a pleomorphic lipoma that occurred on the palm of a 56-year-old woman. An immunohistochemical study, performed to compare the characteristics of a pleomorphic lipoma with those of a spindle cell lipoma, has suggested that the pleomorphic and floret cells of a pleomorphic lipoma are similar to the spindle cells of a spindle cell lipoma and not to fat cells. Our result seems to indicate that these two tumors are on the same spectrum and only the quantity of the pleomorphism keeps them distinct.

Diagnosis, Differential↗
PubMed1988

Purification and identification of [hydroxyprolyl3]bradykinin in ascitic fluid from a patient with gastric cancer.

Kinins in the ascitic fluid from a patient with gastric cancer were purified by gel filtration and reversed-phase high-performance liquid chromatography (HPLC). Two fractions (fractions I and II) showed kinin activity. Fraction I did not correspond to either bradykinin or other known kinins, whereas fraction II corresponded to bradykinin. Fraction I contained 8 amino acid residues from bradykinin minus 1 proline plus 1 additional hydroxyproline. Sequence analysis of fraction I showed that the proline at the third amino acid residue of bradykinin was replaced by hydroxyproline. The retention time of fraction I on reversed-phase HPLC was exactly the same as that of synthetic [hydroxyprolyl3]bradykinin (Arg-Pro-Hyp-Gly-Phe-Ser-Pro-Phe-Arg) and was distinguishable from des-Pro3-bradykinin. Thus, these results demonstrate for the first time the presence of [hydroxyprolyl3]bradykinin in vivo. This is also the first report of the presence of bradykinin in human tumor ascites.

Amino Acid Sequence↗
PubMed1988

Facilitatory effects of caerulein on hypothalamic defensive attack in cats.

Effects of intraventricularly microinjected caerulein (0.1, 1.0 and 10.0 micrograms) on the thresholds for hypothalamically elicited defensive attack and influences of haloperidol (0.5 mg/kg, i.p.) on the effects were studied in chronic cats. Directed attack and hissing were selected for threshold determination, and thresholds for these responses were measured under two situations: one with provocations by a human, and the other without such provocation. Results were as follows. (1) Caerulein lowered all thresholds in generally equal decrements and in a dose-related manner, accompanied by a general behavioral arousal. (2) Prior injection of haloperidol prevented the effects of caerulein, suggesting an antagonism-like interaction between haloperidol and caerulein. (3) Observed facilitatory effects of caerulein on the hypothalamic defensive attack were very similar to those observed with dopamine (DA) agonists such as methamphetamine and apomorphine and opposite to those with DA antagonists such as haloperidol and chlorpromazine. These findings suggest that caerulein exerts its facilitatory effects on the excitability of the ventromedial hypothalamic nucleus through its synergistic interaction with DA.

Aggression↗
PubMed1988

Intermediate lymphocytic lymphoma of the salivary gland.

Three patients had a painless mass in the parotid (two patients) or submandibular regions (one patient). These patients were 70, 66, and 65 years of age, respectively. One patient was male and the other two were female. No symptoms suggesting the presence of autoimmune sialadenitis had been noticed, although one patient suffered from rheumatoid arthritis. Histologic study showed that small atypical lymphoid cells with slightly irregular and indented nuclear contours proliferated diffusely and secondary lymph follicles were distributed at random among them. These findings are similar to those that we recently reported for intermediate lymphocytic lymphoma (ILL) of the thyroid gland arising in patients with autoimmune thyroiditis. A high frequency of ILL among our series of salivary lymphomas (three of nine cases) is discussed.

Aged↗
PubMed1988

Interdomain cleavage of plasma fibronectin by zinc-metalloproteinase from Serratia marcescens.

Limited proteolysis of porcine plasma fibronectin by the 56 kDa proteinase (56K proteinase) (EC 3.4.24.4) from Serratia marcescens released six polypeptides: a 27 kDa peptide, the heparin-binding domain which comprises the NH2-terminal end; a 50 kDa peptide, a mid-molecule that mediates binding to gelatin or collagen; a 160 kDa peptide, that contained the heparin-binding domain with cell-spreading activity; and a 140 and a 20 kDa peptide which released from the 160 kDa peptide. Each fragment was purified and characterized by its chemical and biological properties, and it was found that they were respectively different domains. Both the 160 and the 140 kDa peptide contained one cysteine per mole of peptide. The 160 kDa peptides were connected by a 6 kDa peptide, which was present at the COOH-terminal end of the molecule and was biologically inactive. Only 6 kDa peptide contained a disulfide bond and produced 3 kDa peptide after reduction, whereas other fragments did not change with or without reduction on SDS-polyacrylamide gel electrophoresis. NH2-terminal sequence analyses of the released peptides showed that the 56K proteinase cleaved the fibronectin between the Arg-Thr (located at two different sites), Leu-Ser and Gln-Glu bonds. Out of 118 Arg residues, there are nine sequences containing Arg-Thr, and two of them near or at an interdomain location (at Arg 259 and 2239) were cleaved. Out of 124 Leu residues, there are 11 Leu-Ser sequences and only one, at 687, was cleaved. The above fragments with functional domain activity could be aligned according to the previously reported amino-acid sequence of human or bovine plasma fibronectin. The treatment of fibroblast cells by the 56K proteinase resulted in loss of morphological integrity and extracellular matrix.

Amino Acid Sequence↗
PubMed1988

Purification of a nuclear trans-acting factor involved in the regulated transcription of a human immunoglobulin heavy chain gene.

The expression of the rearranged human immunoglobulin gamma 1 heavy chain gene (HIG1) was shown to be induced through its enhancer by the positive regulatory trans-acting factor(s) that was contained only in cells of B lineage. The trans-acting factors were purified from mouse myeloma NS1 cells, and HIG1-inducing activity was found mainly in fractions of molecular weight 53-127 kd and in a fraction eluted from a heparin-Sepharose column with 0.5 M KCI. This semipurified fraction contained proteins binding to the conserved octamer sequence, ATGCAAAT, in the promoter region, as well as to sequences in the enhancer region. The 0.5 M KCI eluates from a heparin-Sepharose column were applied to a DNA affinity column of synthetic oligonucleotides of the octamer sequence and the sequence TATTTTAGGAAGCAAA in the HpaII-BgIII region of the HIG1 gene enhancer. The protein eluted from the enhancer sequence-specific DNA affinity column showed a strong inducing activity for the HIG1 gene, and the molecular weight of a predominant protein was 96 kd.

Animals↗
PubMed1988

Isolation and identification of hydroxyproline analogues of bradykinin in human urine.

Hydroxyproline (Hyp) analogues of bradykinin and lysyl-bradykinin, in which the third residue of bradykinin, proline, is replaced by hydroxyproline, were isolated from human urine. Their amino acid sequences were confirmed by both amino acid and sequence analyses, and also by comparison of their chromatographic behavior with that of synthetic peptides. The possibility that Lys-Ala3-bradykinin, isolated by Mindroiu et al. [(1986) J. Biol. Chem. 261, 7407-7411] from human urine, was actually Lys-Hyp3-bradykinin is discussed.

Amino Acid Sequence↗
PubMed1988

Human-type blood group activities on chimpanzee erythrocytes with special reference to M and N.

Human-type blood group activities on the red blood cells (RBCs) of three chimpanzees were individually examined with commercial mouse monoclonal antibodies (anti-A, -B, -H, -M, -N, -Lea, and -Leb) as well as lectins (UEA-I and VGA) and conventional polyclonal antisera for the systems ABO, MN, Lewis, Rh-Hr, P, Kell, Kidd, Duffy, and Lutheran. For further analysis of the MN antigens, treatment of the RBCs with sialidase, trypsin, and chymotrypsin were employed. The activities recognized among the three chimpanzees were A, H, M, N, Leb, c, S, k, and Jka. The RBCs of the three individuals possessed the A antigen which showed the same serologic activity as the human A1. Those chimpanzee RBCs showed higher H-activity than the human A1 RBCs. The Lewis b activity was revealed by the absorption-elution method. The RBCs of the three individuals showed a reactivity to the polyclonal anti-M reagents, which was affected by both the sialidase and trypsin treatment. The RBCs of two individuals were agglutinated with the monoclonal anti-N. The receptor was sensitive to sialidase and chymotrypsin. The RBCs of the three individuals, however, did not react with the monoclonal anti-M or with one of the polyclonal anti-N. These results indicate structural differences in the glycophorins and MN antigens between the human and chimpanzee.

ABO Blood-Group System↗