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Biomedical subjects

H Lutz

Publications and source records attributed to H Lutz.

At least 217 records · Page 12Linked to original sources
PubMed1984

Ultrasound tomography of the upper gastrointestinal tract. Orientation and diagnostic possibilities.

40 patients were examined by peroral endoscopic ultrasonography with two new prototypes (Prototype II and III, Olympus Optical Co., Tokyo, Japan). Indications were given by various intestinal diseases. Topographical orientation could be difficult, but was facilitated by standard positions of the instrument. The organs identified included liver, gallbladder, common bile duct, pancreas, spleen and kidneys. Pathological lesions of the mentioned organs were characterized by the reflex pattern known from external ultrasonography. Up till now endoscopic sonography is not a routine method but may give additional information to peroral endoscopy and external ultrasonography without replacing these methods.

Adult↗
PubMed1984

Initial results of transgastric endoscopic ultrasonography in comparison with external ultrasound.

Endoscopic ultrasonography was developed in an attempt to utilize the improved resolution of small-parts ultrasonography also in the investigation of organs located deep within the body as opposed to at the surface. A further expected advantage vis-à-vis external ultrasonography was the avoidance of the frequently obstructing air. In an initial study of 40 patients with a variety of different diseases of the upper abdomen, we were indeed able to utilize the advantages described, with the result that diagnostic information was obtained that neither external ultrasound nor endoscopy was able to provide. A limitation of endoscopic ultrasonography is the fact that the "path" of the transducer is predetermined by the course of the gastrointestinal tract. A further difficulty is the anatomic orientation. To combat this latter problem, our working group has under X-ray control, established standard position for the ultrasound endoscope.

Chronic Disease↗
PubMed1983

[Interdisciplinary diagnosis of choledochus cysts].

Cystic dilatations of the biliary tract are rare anomalies. In the individual case, diagnosis may pose great problems. The relevance of the various methods was assessed in eight patients investigated with different diagnostic methods. In all patients the predominant symptom was uncharacteristic upper abdominal complaints. Two patients showed intermittent jaundice. Among non-invasive methods sonography is preeminent and permits precise demonstration of intra- and extrahepatic biliary duct dilatations. It can be complemented by CAT-scanning. Whereas intravenous cholegraphy does not permit sufficient ascertainment of the diagnosis, ERCP and PTC allow precise demonstration of the anomaly. Functional hepatobiliary scanning is indicated where endoscopic methods are not available. Among the eight patients sonography was decisively relevant in five cases and ERCP in all cases. The remaining functional assessments furnished important additional informations.

Adolescent↗
PubMed1983

Feline oncornavirus-associated cell membrane antigen: a viral and not a cellularly coded transformation-specific antigen of cat lymphomas.

The feline oncornavirus-associated cell membrane antigen (FOCMA) was defined as a tumor antigen common to cat lymphomas and fibrosarcomas induced by feline leukemia virus (FeLV) and feline sarcoma virus (FeSV), respectively. The antigen was recognized by sera from cats thought to be resistant to leukemogenesis. We report here that a common denominator in the activity of naturally occurring viremic cat antisera to FOCMA is, in fact, their reactivity to FeLV C antigenic determinants. The cat antisera, monoclonal antibodies to FOCMA, and monoclonal antibodies to FeLV C, all reacted in immunofluorescence assays with FeLV C-infected cells and immunoprecipitated a molecule electrophoretically indistinguishable from envelope glycoprotein of FeLV. Viremic cat antisera to FOCMA bound to budding virus particles of FeLV C-infected cells, even though some of them could not be absorbed by mature virion proteins. Thus, the unusual feature of cat antibodies to FOCMA is their binding to nascent but not to mature virus particles. FOCMA-positive cat lymphomas expressed antigenic determinants of FeLV-C gp70, with or without productive infection. FeLV-negative tumors not expressing FeLV C gp70 were also FOCMA negative. Furthermore, most of the viremic cat sera and the monoclonal antibodies to FOCMA did not react with FeSV-transformed nonproducer cells. The absence of FOCMA from these cells and from FeLV-negative lymphoid tumors and its presence in FeLV-C infected fibroblasts indicated that this antigen is virus encoded and not a cellular tumor-specific antigen.

Animals↗
PubMed1983

Monoclonal antibodies to three epitopic regions of feline leukemia virus p27 and their use in enzyme-linked immunosorbent assay of p27.

Three different monoclonal antibodies were developed against the major core protein (p27) of feline leukemia virus (FeLV). Each antibody was directed against a different epitope of the species-specific portion of FeLV-p27. The 3 antibodies reacted with 5 different isolates of FeLV but not with 7 other retroviruses (MuLV (Rauscher), MuLV (AKR), MPMV, MMTV, SMRV, BAEV, RD 114). These monoclonal antibodies could readily be adapted to an enzyme-linked immunosorbent assay (ELISA) for the specific measurement of FeLV-p27. When compared in an ELISA with conventional reagents, the battery of monoclonal antibodies proved to be as sensitive as conventional polyclonal antibodies.

Animals↗
PubMed1983

Efficacy of triclabendazole against Fasciola hepatica in sheep and goats.

Triclabendazole (Fasinex), a new fasciolicide of the benzimidazole group, was tested in sheep and goats. A controlled test with 24 artificially-infected sheep revealed a 100% efficacy of triclabendazole at a dose of 10 mg kg-1 body weight against Fasciola hepatica aged 4 and 13 weeks, respectively. In naturally-acquired severe subacute to chronic fascioliasis in 66 sheep and 10 goats, the drug was highly effective in three trials when applied at 10 mg kg-1 in sheep and at 5 mg kg-1 in goats. Triclabendazole was well tolerated, whereas side effects occurred in one trial with niclofolan (Bilevon-M) (3 mg kg-1) which was used for comparison.

Animals↗
PubMed1983

[Ultrasound diagnosis via the gastroscope].

Eighty patients presenting with various diseases of the upper abdominal organs were investigated in an initial series of examinations performed with an ultrasonic endoscope provided with a mechanical sector scanner (7.5 MHz rated frequency, sector selectable between 90 degrees and 180 degrees), and/or a linear array device (7.0 MHz rated frequency, field of view 31 mm). Orientation, which was initially difficult, was facilitated by establishing standard positions. The pancreas, liver, gallbladder, bile duct, spleen, the two kidneys, the vessels of the upper abdomen, the wall of the stomach and, of course, the heart, are all accessible to endoscopic diagnostic ultrasound. At the present state of our knowledge, the method does not appear suitable for the systematic diagnostic work-up of the upper abdominal organs, but in certain cases it would seem to be a useful supplementary examination to external ultrasonography or endoscopy.

Abdomen↗
PubMed1983

An improved method for in situ freezing of cat brain for metabolic studies.

This study introduces a new method for rapid freezing of the cat brain. The method employed a Styrofoam box which was fitted around the head of the animal. Liquid nitrogen was poured into the box until the head was submerged. Temperature changes in three brain sites (ventral hypothalamus, the fourth ventricle, and the corpus callosum) and levels of labile carbohydrate metabolites (glycogen, glucose, ATP, P-creatine, and lactate) in five brain regions (cortex, thalamus, midbrain, cerebellum, and pons) frozen by the box method were compared with those frozen by a conventional cup method in which liquid nitrogen was poured into a hollow Styrofoam cup placed on top of the skull. The box method shortened the time of arrival of the freezing front and improved the freezing rate. The time required to bring the tissue to -20 degrees C was shortened, from 20 min at the ventral hypothalamus and 10-12 min at the fourth ventricle with the cup method, to less than 5 min at both sites with the box technique. Continued perfusion of brainstem prior to freezing was demonstrated. Levels of metabolites frozen by either method were similar. Lactate levels in any of the five brain regions studied by either method were not elevated, indicating no ischemic change.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1983

Granules of blood eosinophils are stained directly by anti-immunoglobulin fluorescein isothiocyanate conjugates.

Direct staining of the granules of blood eosinophils by anti-immunoglobulin fluorescein isothiocyanate (FITC) conjugates was observed when feline blood smears were tested for presence of feline leukemia virus (FeLV) antigen by immunofluorescent antibody. When blood smears of other species including swine, horses, cattle, dogs, sheep, birds, and human beings were examined, direct staining of eosinophils by FITC conjugates was also detected. This FITC staining was restricted to eosinophils and was not observed in neutrophils, lymphocytes, and platelets. Direct FITC staining of eosinophils does not represent a problem in immunofluorescent test for the detection of FeLV infection in cats, as long as the eosinophils, which can easily be recognized as such, are excluded from the spectrum of interpreted cells.

Animals↗
PubMed1983

Course of feline leukemia virus infection and its detection by enzyme-linked immunosorbent assay and monoclonal antibodies.

Monoclonal antibodies specific for 3 distinct epitopes of the species-specific determinants of feline leukemia virus (FeLV) p27 were used in an enzyme-linked immunosorbent assay (ELISA) for measurement of serum p27 in cats infected with FeLV. Group-specific antigen (GSA) of FeLV in peripheral blood leukocytes was also determined by an immunofluorescence assay. Antibodies to FeLV and the feline oncornavirus-associated cell membrane antigen (FOCMA) were also measured. Thirty-six cats were surveyed and assigned to 4 categories. Five developed persistent viremia (category 1), characterized by continuous expression of p27, GSA, and low antibody titers to FeLV and FOCMA. Eleven cats with transient viremia (category 2) and 13 cats that were never detectably viremic (category 3), as judged by absence of GSA and p27, developed increased antibody titers to FeLV and FOCMA. Seven cats were never viremic, as judged by the GSA in the peripheral blood leukocytes, but still had detectable serum p27 (category 4). Most category 4 cats developed high antibody titers against FOCMA and/or FeLV. Of 307 field cats examined, 7% of the healthy cats and 10% of the sick cats could be assigned to category 4. However, this difference was not significant (P greater than or equal to 0.05). Of 26 cats with neoplasms 2 (1 of 12 with lymphosarcoma) could be classified as category 4. Because virus could be isolated from 2 category 4 cats, they were considered immune carriers.

Animals↗
PubMed1983

[Effect of different infusion regimens on the hormonal and metabolic reaction before, during and after cholecystectomy].

18 metabolically healthy female patients with cholelithiasis, who had to undergo cholecystectomy, were divided into 3 groups and studied for a 4-day period. Group I, n = 6, received beginning on the operationday 0,19 g/kg BW X h of a polyol-mixture-solution (Sorbitol/Xylitol 1:1) and 0,86 g 1-crystalline amino acids (AA)/kg BW X day. Group II, n = 6, received beginning 24 h preoperatively only 1,14 g 1-crystalline AA/kg BW X day. Group III, n = 6, received beginning 24 h preoperatively 0,07 g Xylitol/kg BW X h, 0,03 g Glucose/kg BW X h and 1,14 g 1-crystalline AA/kg BW X day. In this study we could demonstrate that the pre-, intra- and postoperative hormonal and metabolic response is strongly influenced by different infusion regimens. Only by the simultaneous infusion of carbohydrate together with AA during the preoperative period a significant drop of triiodothyronine can be avoided. The rise of insulin and cortisol in the early postoperative period is augmented by the infusion of carbohydrates together with AA. There was no difference in growth hormone response to surgery between group I-III. All 3 groups showed a continuous fall of cholesterol during the study period.

Cholecystectomy↗
PubMed1983

Transgastric sonography in pancreatic diseases.

The possibilities of a transgastric ultrasonic diagnosis of the pancreas and other organs are investigated in an initial series of 50 examinations using two different ultrasonic endoscopes.

Endoscopy↗
PubMed1983

[Is the demand for a preoperative routine examination program justified?].

In a retrospective study with 136, 943 general and regional anaesthesias we demonstrated that the frequency of intraoperative cardiovascular complications is directly connected to the quality of preoperative examination and therapy proceedings. In addition to a careful preoperative clinical examination, a routine examination programme (REP) therefore should be carried out which determines relevant functional disorders of the essential organs and which gives the essential condition for an optimal preparation for surgery.

Anesthesia, Conduction↗