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Biomedical subjects

H Ludwig

Publications and source records attributed to H Ludwig.

At least 469 records · Page 26Linked to original sources
PubMed1975

Human endometrial fluid kinetics as observed by scanning electron microscopy.

Segments of human endometrium, obtained during different stages of the menstrual cycle, were fixed in glutaraldehyde, processed by critical point drying, coated with carbon and gold, than observed with a scanning electron microscope under magnifications varying from 20 to 200,000. The endometrium was basically made of two different types of cells: secretory nonciliated cells and ciliated cells. Different types of secretory cells at different stages of secretory cycles were observed. The endometrial secretion is apocrine: the apical cell membrane of the endometrial cell ruptures, releasing secretory material. The rupture of cells within a given segment of the endometrium is asynchronous. Development of aptical microvilli, synthesis, storage, and release of endometrial secretory granules, and ciliogenesis are hormone dependent. The response of the ciliated and secretory cells of the endometrium varies throughout the menstrual cycle. This is particularly noted in cell specialization, the development of apical microcilli, and ciliogenesis.

Body Fluids↗
PubMed1975

[Clinical and immunological studies in idiopathic adrenal insufficiency].

The application of 2 special methods to the pathogenetic elucidation of idiopathic Addison's disease is discussed in the light of a clinical case. In accordance with the literature, this patient with idiopathic Addison's disease showed a normal adrenomedullary response (as gauged by the catecholamine excretion and the increase in plasma renin activity) to 2-deoxy-D-glucose administration. Moreover, IgG antibodies were demonstrable in the patient's serum with respect to adrenocortical antigens of the cytoplasmic components of the endocrine cells of the zona glomerulosa and zona fisciculata.

Adrenal Glands↗
PubMed1975

[Thyreotoxic crisis triggered by stimulation of the adrenal gland medulla].

Thyroid storm was observed in a patient with Grave's disease after stimulation of release of epinephrine and norepinephrine with 2-DG. Measurements of catecholamine secretion before and during onset of thyrotoxic crisis suggested the important bearing of increased activity of the sympathetic nervous system on pathogenesis of thyroid storm.

Adrenal Glands↗
PubMed1975

Repetitive sequences in complete and defective genomes of Herpesvirus saimiri.

Two types of Herpesvirus saimiri genomes can be isolated from purified virions: (i) the M genome is a double-stranded, liniear DNA molecule with a mean contour length corresponding to 89 times 10-6 daltons. The M genome contains about 70% of unique sequences (light DNA, 36% guanine plus cytosine) and 30% reiterated sequences (heavy DNA, 71% guanine plus cytosine). (ii) the H genome is composed of heavy DNA only and is more heterogeneous in size. The sequences in the H genome are up to 40-fold reiterated, indicating defectiveness of this type of genome. The repetitions in the H genome and the M genome cross-hybridize almost completely and have identical kinetic complexity (2.8 times 10-6 daltons). DNA infectivity studies by using the calcium phosphate and the DEAE-dextran method gave further evidence that H genomes are defective: no infectious virus was recovered from permissive cells treated with heavy DNA, whereas M genome-infected cells developed cytopathic changes after 11 to 56 days. Defective H genomes were present in the progeny virus two passages after transfection.

Animals↗
PubMed1975

Effect of 2-deoxy-D-glucose on herpesvirus-induced inhibition of cellular DNA synthesis.

In pseudorabies virus-infected cells host DNA synthesis is turned off 4 to 5 h postinfection. In the presence of 0.5 mM 2-deoxy-D-glucose, however, synthesis of both cellular and viral DNA proceeds unimpaired throughout the virus replication cycle. The uptake of radioactive thymidine into mock-infected cells is not altered in the presence of 2-deoxy-D-glucose. Virus-specific protein synthesis and particle formation also proceed in medium containing the deoxy sugar, but the virus particles produced are noninfectious and cell fusion is inhibited.

Animals↗
PubMed1975

Bladder cancer: results of radiation therapy in 384 patients.

Between 1957 and 1972, 384 patients with bladder cancers were treated initially with megavoltage radiation therapy. Actuarial five-year survival ranged from 35 to 42% for Stages A and B1 tumors, and was 35, 22 and 7%, respectively, for Stages B2, C and D carcinomas. Approximately 30-40% of deeply invasive tumors confined to the bladder can be controlled with radiation therapy alone, directed solely to the bladder itself.

Aged↗
PubMed1975

[Autoimmune phenomena in D-penicillamine therapy].

In two patients with rheumatoid arthritis, immunoglobuline deposits in the skin were detected during D-penicillamine treatment. One patient presented with drug induced exanthema, the other with pemphigus erythematosus which was confirmed by clinical, histological, and immunofluorescent findings. During the development of the specific side-effect in one patient, an increase of ANA-titre and antibody-binding-capacity versus denaturated DNA was observed. Therefore in a prospective study of another ten patients during four months after onset of treatment with D-penicillamine, antinuclear antibodies and antibody-binding-capacity versus native and denaturated DNA were examined. The latter group of patients - without skin lesions - did not show any changes in the above mentioned parameters.

Antibodies, Antinuclear↗
PubMed1975

HL-A antigens in idiopathic Addison's disease.

The frequencies of 36 HL-A antigens of the SD-1, SD-2 and SD-3 locus have been determined in 20 patients with idiopathic Addison's disease and compared with those of 450 controls. No significantly different HL-A antigen frequencies could be observed in both groups. These findings are in contradiction to data reported by a Copenhagen group. Possible mechanisms which might have led to this discrepancy are discussed.

Addison Disease↗
PubMed1975

Collagen-induced acute synovitis in collagen-immunized rabbits.

Thirty rabbits were investigated in 3 groups: a) one consisting of non-immunized animsls which were injected intraarticularly with human soluble collagen, human gammaglobulin or saline, b) the other consisting of human gammaglobulin-immunized animals which were injected with human soluble collagen and human gammaglobulin into the knee joints, and c) the third consisting of human collagen-immunized rabbits which were injected with human soluble collagen and human gammaglobulin into the knee joints. Eighteen hours after injection animals were sacrificed, joint fluid collected, cell number in fluid determined and synovial tissue prepared for histologic investigation. Distinct acute synovitis was observed in collagen injected knees of collagen immunized rabbits, as shown by distinctly increased cell number in fluids and histologic investigation. In comparison to collagen- or gammaglobulin injected knees of non-immunized animals, increase in cell number of collagen injected knees of collagen injected animals was statistically significant with P less than 0.005 respectively with P less than 0.01, and with P less than 0.025 in comparison to gammaglobulin injected contralateral knee. Distinct acute inflammation was observed morphologically in synovial tissue of collagen injected knees of collagen immunized animals only. Acute synovitis was also observed in positive controls, i.e. gammaglobulin injected knees of gammaglobulin immunized animals. These observations show, that appearance of collagen in molecular disposable form can induce acute inflammation of joints in state of collagen immunization.

Acute Disease↗