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Biomedical subjects

H Lindemann

Publications and source records attributed to H Lindemann.

67 records · Page 4Linked to original sources
PubMed1980

[Interaction of the three alkylating drugs, cyclophosphamide, ifosfamide and trofosfamide, with DNA and DNA-constituents in vitro (author's transl)].

In vitro experiments were done for investigating the interactions of the three 3H-labelled alkylating drugs cyclophosphamide, ifosfamide and trofosfamide with DNA and DNA-constituents. Evidence is presented for a preferred reaction with orthophosphate groups, leading to phosphotriesters in the DNA. The results are discussed in view of the chemotherapeutic action of the drugs their mutagenic or carcinogenic effects.

Alkylating Agents↗
PubMed1980

[Increase of heart rate and determination of W170 as a measure of physical working capacity in children 5 to 14 years of age (author's transl)].

1. Exercise testing with stepwise increasing workload was performed in 257 healthy children (134 boys, 123 girls) on a bicycle ergometer, which was independent of variations in pedal speed within a certain range. The procedure of exercise was adapted to the children as far as possible. 2. Nomograms and standard values for heart rate during exercise and for physical working capacity at a heart rate of 170/min (W170) were established. The increase of heart rate (from 1.0 to 2.5 Watt/kg) was well correlated to the age, length, body weight and body surface. If heart rate, respectively W170 are related to workload per kg body weight, results are dependent on sex, but not on age. 3. By means of the new standard values a rough evaluation of cardiorespiratory condition is possible, if the heart rate is measured at moderate exercise during steady state. A more precise assessment of the cardio-respiratory fitness is granted by the W170. By this method physical working capacity can be determined in children with heart diseases in a relative simple manner. 4. Normally in stepwise increasing loads the exercise takes 6 minutes per workload. If this time is reduced, an underestimation of the expected heart rate, respectively an overestimation of W170 is observed, which depends on the extent of the shortening of exercise.

Adolescent↗
PubMed1979

[Replacement of mitral and aortic valve of a 12 years old boy (author's transl)].

Report of the case of a 12 years old boy, who because of acquired mitral valve insufficiency (grades II--III), after an illness of virus-carditis, became, in the first instance, a Bjoerk-Shiley-Ventil M27 prosthetic valvular replacement, and after 3 months, in one sitting, correction of a paravalvular leakage and prosthetic valvular replacement of the insufficient aortic valve with a Bjoerk-Shiley A23. The boy was well rehabilitated one year after the last operation. This is supported by the similar results of the echocardiographic, the ergometric and the pulmonary function tests carried out. The size of the replaced prosthetic valve makes the long time prognosis favourable. Double-valvular prosthetic replacement in children was hitherto carried out predominantly as a result of rheumatic diseases. The rheumatic process as well as congenital valvular malformation could surely be excluded in our patient so that the virus-process seems to be the most likely cause of the valvular defect.

Aortic Valve↗
PubMed1979

Body plethysmographic measurements in children with an accompanying adult.

If a child refuses to enter the body plethysmograph, he may be accompanied by a relative or an assistant. Measurements are possible after the companion has stopped breathing and box pressure has stabilized. The reliability of this procedure is proved by a comparative examination of 25 children performed without and with a companion.

Adolescent↗
PubMed1977

Primary structure of protein S13 from the small subunit of escherichia coli ribosomes.

The experimental details which led to the determination of the complete primary structure of protein S13 from the small subunit of Escherichia coli ribosomes are presented. S13 consists of 117 amino acid residues and has the following composition: Asp6, Asn2, Thr6, Ser6, Glu6, Gln2, Pro4, Gly11, Ala11, Cys1, Val7, Met2, Ile12, Leu9, Tyr2, Phe1, His3, Lys11 and Arg15. Tryptophan was not found. The molecular weight of protein S13 as derived from the sequence shown in Fig. 1 is 12970. The amino acid sequence of the protein was determined by combining the results obtained from liquid phase Edman degradation of the intact protein with those from the peptides isolated after enzymatic digestions with trypsin, Staphylococcus aureus protease and thermolysin. Additional information about the primary structure was derived from analysis of the chymotryptic peptides of protein S13 and from its digestion with carboxypeptidase C. The amino acid sequence of protein S13 was compared with the published sequences of the other ribosomal proteins of E. coli and predictions for the secondary structure of this protein were made.

Amino Acid Sequence↗
PubMedDate not supplied

[A modified ultra-rush-protocol of allergen immunotherapy in children and adolescents with insect venom allergy].

BACKGROUND: In Germany the prevalence of insect venom allergy in the population is about 5 %, 10-40 deaths are reported every year. With a success rate of 95 % venom immunotherapy (SIT) has a convincing protective effect. The purpose of this study was to investigate the safety of the original ultra-rush-protocol in children and reduce from 9 to 8 doses by avoiding a second application of 100 micro g insect venom. METHODS: Nineteen children and adolescents with insect venom allergy were treated according to the modified ultra-rush-protocol. The first 5 children were hyposensitized according to the original protocol suggested by Brehler et al., the remaining 14 children (age 6-18 years) received only 8 injections (0.01/0.1/1/10/20/40/80/100 micro g). 5 patients were discharged on the second day four hours after the final application of 100 micro g insect venom. RESULTS: Extensive reddening due to inflammation was found in injection spot in 5 patients. Four patients had an amplified wheal formation. No systemic reactions were observed. The maintenance therapy was well tolerated. CONCLUSION: The modified ultra-rush-protocol increases compliance by short inpatient stay. In addition the modified hyposensitization was sure and well tolerated.

Adolescent↗
PubMedDate not supplied

Is hysteroscopy of value in the investigation of female infertility?

A prospective multicentre study was designed to ascertain the value of hysteroscopy in the evaluation of female infertility. The study comprises 619 CO2 hysteroscopies in women complaining of infertility. Four hundred and ninety nine examinations (80%) were performed without anaesthesia or cervical dilatation. Twenty eight cases of malformation were diagnosed. Adhesions were found in 68 patients (11%), polypi in 56 (9.1%) and submucous fibroids in 51 (8.2%). Endometrial hyperplasia was diagnosed by hysteroscopy in 67 patients, but there was only 42% histological correlation. Examination of the tubal ostia by hysteroscopy revealed 26 patients (4%) with unilateral tubocornual polyps and one with bilateral polyps. The correlation with hysterosalpingography (HSG) in 185 patients was poor. In 20% where the HSG was reported as normal there were hysteroscopically demonstrable lesions whereas there were false positive findings at HSG in 35% of cases. Hysteroscopy is the only technique which provides an accurate clinical evaluation of the uterine cavity although it does not provide significant information about the cervical canal or fallopian tubes.

Adult↗
PubMedDate not supplied

Interaction of cyclophosphamide with DNA in isolated rat liver cell nuclei.

Isolated rat liver cell nuclei were incubated with the alkylating cytostatic drug cyclophosphamide (CPA) in the presence of a microsomal activation system. Digestion of the 3H-CPA-treated nuclei with DNase I and micrococcal nuclease, respectively, showed that the CPA-modified DNA apparently has become resistant against such enzymatic attack. For analysis of the DNA-CPA reaction products, the DNA was isolated under mild conditions and degraded enzymatically. In cell nuclei whose DNA had been prelabeled with 32P in vivo, a predominant binding of 3H-CPA (about 50%) to the DNA phosphate groups was observed. Terminal phosphate groups apparently play an important role in this reaction. Neutral heating of DNA from 3H-CPA-treated nuclei liberated up to 60% of the initially bound 3H-radioactivity. The results are discussed in relation to the recent data concerning the route of decomposition of activated CPA to phosphoramide mustard and acrolein.

Animals↗