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Biomedical subjects

H Li

Publications and source records attributed to H Li.

At least 1,171 records · Page 65Linked to original sources
PubMed1998

[The relationship between different serological types of Pseudomonas aeruginosa strain in lower respiratory tract and its clinical significance].

OBJECTIVE: To explore the relationship between different serological types of pseudomonas aeruginose (PA) in sputum and its clinical significance. METHOD: PA was detected by Vitek-Ams GNI card and its sensitivity to 12 kinds of antibiotics was measured with GNS-GA, its different serological types were assayed with standard diagnostic serum of type 20. RESULT: 14 different serological types were isolated from 101 strains of PA in sputum (with the typed rate 84.2%). Among them, type 6, type 1, and type 3 were higher with 23.7%, 13.9% and 10.9% respectively. But another 15.8% of strains couldn't be differentiated, so called untyped PA group, among them 10 strains with mucoid-like colony (63% of this untyped group). Patients suffered with this group were older and were very seriously infected with higher mortality (13%). They often suffered from serious COPD and bronchiectasis. The sensitivities of all PA strains to antibiotics in vitro as follows: tobramycin 92.0%, ceftazidine 89.1%, ciprofloxacin 87.1%, ticarcillin 82.2%, and gentamycin 76.2%. Among 12 PA strains tested with imipenem sensitive test, only one strain of mucoid-like colony had been found resistant. CONCLUSION: Identification of serological type of PA can be used as an important method to make out if the infection comes from the same source, and for surveillance of hospital infection, and to show the main types of PA in some district. But the sensitivity of antibiotics in vitro is no definitive correlation with PA serological types. The disease infected with untyped PA strains have its clinically special characteristics and must be kept attention.

Adult↗
PubMed1998

[Quantitative analysis of vestibulo-ocular reflex and optokinetic reflex electronystagmography in patients with unilateral loss of vestibular function].

OBJECTIVE: To study changes of the vestibular function in the process of vestibular compensation. METHODS: The electronystagmography recording of spontaneous nystagmus(SN) with tests for vestibulo-ocular reflex (VOR) and optokinetic reflex (OKR) were performed in 105 patients with unilateral loss of vestibular function, the results were analysed quantitatively. RESULTS: (1) Twenty-one patients with courses from 3 days to 6 years presented a SN directed towards the intact side, with an average slow phase velocity (ASPV) of 7.12-12.07 degrees/s. (2) The intensity of VOR nystagmus as a response to the warm-cold water stimulation to the intact ear was lower than that of normal subjects. (3) By 0.167 Hz, 90 degrees sinusional oscillation stimulation, the ASPVs of the patients' VOR nystagmus directed towards the intact and affected sides were lower than that of the normal. A predominance of VOR nystagmus rotation towards the intact side was found in patients with courses < or = 5 years. There was no predominance of VOR nystagmus found in patients with courses > 5 years. (4) There was no significant difference between the optokinetic nystagmus (OKN) intensity of the normal persons and that of patients with lesions outside the cerebellopontine angle and with tumors in cerebellopontine angle which was < 2.5 cm in diameter. The OKN intensity of patients with tumors in cerebellopotine angle with diameters > or = 2.5 cm was lower than that of normal subjects. A predominance of OKN towards the intactside was found in these patients. CONCLUSION: The vestibular function is in inhibitory and the vestibular system becomes rebalanced due to this inhibition in the process of vestibular compensation.

Adolescent↗
PubMed1998

[Enantiomeric high performance liquid chromatographic separation of epinephrine and analogous using chiral mobile phase additives].

The enantiomeric resolution of epinephrine, isoproterenol, ephedrine was studied by reversed phase HPLC. beta-CD, DM-beta-CD, TM-beta-CD were used as chiral mobile phase additives. The effects of the concentration of DM-beta-CD and the concentration of methanol on resolution of D,L-epinephrine were investigated. The results showed: D,L-epinephrine and D,L-isoproterenol can be separated by beta-CD, DM-beta-CD, TM-beta-CD, but D,L-ephedrine can't. Hydrogen bonding plays an important role in the chiral recognition mechanism. In the separation of isoproterenol, the enantioselectivity of TM-beta-CD was better than those of beta-CD and DM-beta-CD. As the concentration of DM-beta-CD changed from 0.04 mmol/L to 1.00 mmol/L, the resolution varied slightly. The experiment of the effect of methanol concentration on Rs showed: when the ratio of methanol/water was 40/60, the Rs reached the maximum. The coefficients of variation of retention time of D-epinephrine and L-epinephrine were 1.3% and 1.4% respectively. It is demonstrated that the chromatographic systems with a dynamically-generated stationary phase with methylated-beta-cyclodextrin are versatile tools for enantiomer separation. This technique leads to column with excellent time stability and good reproducibility of the enantio-selectivity.

Adrenergic Agents↗
PubMed1998

[Bioassay of TNF-alpha in synovial fluid and ultrastructural observation of synovium from patients with osteoarthritis].

OBJECTIVE: To better define the role of synovium in the pathogenesis of osteoarthritis (OA). METHODS: We measured the bioactivity of tumor necrosis factor-alpha(TNF-alpha) in synovial fluid (SF) and examined the histologic and ultrastructural features of the synovium from patients with OA and from patients with acute and chronic traumatologic joint. RESULTS: The results showed that the levels of TNF-alpha in SF from OA were significantly elevated. Marked fibrosis and thickened intima were found in OA synovium, with much dense collagen, which, in some cases, was banded, but no specific inflammatory changes were detected. Type B cells in OA synovium had the characteristics of organelles of cells active in synthesizing and secreting protein. Type A cells in OA synovium had the features of the macrophage. CONCLUSION: Evident fibrosis in synovium may be either mediated by the cartilage degradation product or by the enhanced joint damage process in OA.

Aged↗
PubMed1998

[Expression of nitric oxide synthase in squamous cell carcinoma of tongue and its prognosis].

OBJECTIVE: To study the expression of nitric oxide synthase (NOS) in squamous cell carcinoma of tongue and its prognosis. METHODS: The NOSI, NOSII and NOSIII of 55 cases of squamous cell carcinoma of tongue were detected by immunohistochemistry. RESULTS: The expressive rates of NOSI, NOSII and NOSIII were 30.9%, 40.0% and 38.2% respectively. There were significant differences between the patients with and without neck nodal metastasis, and between the patients survival more than 5 years and less than 5 years (P < 0.01), There were differences between G1, G2 and G3 and the clinical stages in NOSII (P < 0.05). CONCLUSION: The expression of the NOSI, NOSII and NOSIII in squamous cell carcinoma of tongue is raleted to its prognosis and the NOSII plays a more important role in growth, differentiation, metastatic capability and prognosis of the tumor.

Adult↗
PubMed1998

[The orthogonal design study on tumor-inhibition and cancer-block role of Chinese medicinal 1023 co].

OBJECTIVE: To study the effect of Chinese herb medicine on the inhibition of tumor formation and block of malignant transformation. METHODS: The Chinese medicine 1023 co. was given by intubation feeding started simultaneously with the painting of 0.5% DMBA on the hamster's cheek mucosa. RESULTS: The tumor formation in 1023 co. group was delayed for 12 days comparing with the control group and the incidence of dysplasia and cancer in 1023 co. group was lower significantly than that of the control group (P < 0.01). CONCLUSION: The 1023 co. is effective in inhibiting tumor formation and blocking cancerous transformation. By orthogonal design we find the proper prescription is A2 B2 C2 D1.

Animals↗
PubMed1998

[Apoptosis of renal cell carcinoma and apoptotic related genes bcl-2 and bax].

OBJECTIVE: To explore the mechanism of renal cell carcinoma (RCC). METHOD: We detected the apoptotic status and the expression of apoptotic related gene bcl-2 and bax protein in 48 cases of RCC with TdT-mediated dUTP-biotin nick end labeling (TUNEL) and immunohistochemistry methods. RESULT: 22 cases showed low expression of bcl-2 protein with an average apoptotic index (AI) of 1.35 and 26 cases had high expression of bcl-2 with an AI of 1.04 (P < 0.05). 34 cases showed low expression of bax protein with average AI of 1.03 and 14 cases had high expression of bax protein with an average AI of 1.63 (P < 0.01). The stronger bcl-2 expression, the weaker bax expression and the less number of apoptotic cells. CONCLUSION: both bcl-2 and bax take part in the occurrence and progression of RCC.

Adolescent↗
PubMed1998

[Expression and significance of Fas/APO-1 and bcl-2 protein in renal cell carcinoma].

OBJECTIVE: To investigate the role of Fas/APO-1 and bcl-2 in occurrence and progression of renal cell carcinoma. METHOD: Immunohistochemistry method was used to detect the expression of Fas/APO-1 and bcl-2 protein in 35 cases of renal cell carcinoma tissues and 26 cases of normal renal tissues. RESULT: The expression rate of Fas/APO-1 protein in renal cell carcinoma tissues was 57.14%, significantly lower than that in normal renal tissues (84.62%) (P < 0.05), and the intensity of expression was also lower. The expression rate of bcl-2 protein reached 80.00%, much higher than that in normal renal tissues (53.85%) (P < 0.05). CONCLUSION: Both Fas/APO-1 and bcl-2 take part in the occurrence and progression of renal cell carcinoma.

Adult↗
PubMed1998

[Association of nm23-H1 with orthopedic oncological conditions].

OBJECTIVE: To explore the correlation between nm23-H1 gene and malignant and semi-malignant bone tumors. METHOD: Thirty surgical specimens were collected, including 8 osteosarcomas, 7 giant cell tumors, 4 chondrosarcomas, 2 Ewing's sarcomas, 2 malignant fibrohistocytomas, 1 neurofibrosarcoma, 1 schwannoma, and 5 metastatic cancers. The expression of nm23-H1 in these specimens was observed by in situ hybridization and immunohistochemical staining. RESULT: The expression of nm23-H1 in 4 cases of primary giant cell tumor (GCT) was high, but was low or absent in 3 recurrent ones. In 4 cases of chondrosarcomas, the expression was positive. Its expression was basically negative in Ewing's tumor, malignant fibrous histiocytoma and metastatic sarcomas. CONCLUSION: There is no association of nm23-H1 with osteosarcoma.

Adult↗
PubMed1998

[Corneal epithelium implantation under corneal flap after excimer laser in situ keratomileusis: a clinical pathological and cytological study and management].

OBJECTIVE: To study the clinical pathological and cytological characteristics of corneal epithelial cells implanted under the cap of laser in situ keratomileusis and the method of management. METHODS: For 4 eyes with epithelial cell implantation, the corneal cap was torn from temporal side to nasal, and the implanted material was removed from the flap and the stromal base. After washing between the interface, the cap was repositioned. RESULT: No epithelial implantation occurred again in these 4 cases after follow-up for 1 similar 12 months. The clinical pathological and cytological studies showed the material removed was degenerated and necrotic epithelial cells mixed with some unknown materials. CONCLUSION: Clinically, the material formed under the cap white and yellow in color should be removed as soon as it happens. The material consists mainly of non-structural material with necrotic and degenerated epithelial cells.

Cornea↗
PubMed1998

[Observation of anterior chamber angle with ultrasonic biomicroscopy after posterior chamber implantation of intraocular lens].

OBJECTIVE: To observe the changes of anterior chamber angle after extracapsular cataract extraction (ECCE) with posterior chamber implantation of intraocular lens (IOL) and discuss the associated factors. METHODS: The changes of anterior chamber angle in 31 cases (41 eyes) with senile cataract were observed with ultrasonic biomicroscopy after ECCE and posterior chamber implantation of IOL. RESULTS: (1) There were 6 eyes with peripheral anterior synechia of the iris, 12 eyes with abnormal pupillary configuration, which were related to the position of IOL haptics. (2) The IOL haptics fixed out of the capsular bag were not all fixed at the ciliary grooves. (3) After postoperative 3 months, the residual cortex of the lens still existed. Too much residual cortex in the eye caused postoperative peripheral iris bombe and narrowing of chamber angle. CONCLUSION: The changes of anterior chamber angle after posterior chamber implantation of IOL are related to the position of IOL haptics and much residual cortex must be avoided.

Adult↗
PubMed1998

[Effects of mitomycin C on haze after photorefractive keratectomy for myopia in rabbits].

OBJECTIVE: To investigate the effects of mitomycin C on haze after photorefractive keratectomy. METHODS: Forty-five rabbits underwent bilateral 193 nm excimer laser photorefractive keratectomy to correct 8 diopters of myopia. All eyes were allocated randomly to be treated with 0.008% mitomycin C during operation, or 0.1% dexamethasone after operation, or no medical treatment as control. Clinical and histopathologic examinations were made with slit-lamp microscope, specular microscope, light microscope and transmission electron microscope. RESULTS: At 4, 8 weeks after operation, the corneal haze was significantly less in mitomycin C group than that in the control group or in dexamethasone group, and the corneal haze was significantly less in dexamethasone group than that in the control group. At 1, 4, 8 weeks, the number of keratocyte in the anterior stroma of ablation area was significantly less in mitomycin C group than in the control group or in dexamethasone group. The difference between dexamethasone group and the control group was insignificant. The changes in time of reepithelialization, thickness of epithelium and keratocyte number of anterior stroma in ablation area were statistically insignificant among all groups. CONCLUSION: Mitomycin C can reduce corneal haze by inhibiting the proliferation of keratocyte and has no toxicity on cornea. Mitomycin C is more effective than dexamethasone on haze.

Animals↗
PubMed1998

[Determination of Cd, Cu, Pb, Hg by reversed-phase high performance liquid chromatography].

The chromatographic behaviors of Me(n+)-Dz (dithizone) have been studied with RP-HPLC. A method for the determination of Cd, Cu, Pb and Hg has been established. The chromatographic conditions were: column: Shim-pack CLC-ODS, 150 mm x 6.0 mm i.d.; mobile phase: V(methanol): V(water): V (chloroform) (containing volume fraction 1% triethylamine) = 80:12:8; flow rate: 1 mL/min; column temperature: 35 degrees C; detection wavelength: 254 nm. The linear ranges were from 0.01 mg/mL to 2.0 mg/mL with correlation coefficients of 0.9993-0.9998. The detection limits of Cd, Cu, Pb, Hg were from 2.4 micrograms/L to 5.0 micrograms/L. The RSDs were in the range from 1.8% to 9.7%, and the recoveries ranged from 94% to 103% (except Hg). The method has been applied to the analysis of hair.

Cadmium↗
PubMed1998

[Comparative observation on the efficacy of several test/probe systems for detecting schistosome circulating antigen].

AIM: To compare the efficacy of 5 test/probe systems for the detecting of schistosomal circulation antigen (SCA) in sera from infected rabbits. METHODS: Nine rabbits were infected with monosexual cercariae (either male or female), 7 rabbits were infected with bisexual cercariae as controls. Blood samples were collected periodically post infection (p.i). 15 rabbits were infected each with 250 bisexual cercaria and divided into 3 groups, 2 groups were treated with allyl thiourea (295-590 mg/kg) from 19 d or 46 d respectively, to inhibit their egg formation. One untreated group was used as control. Blood samples were collected weekly until 8 wk post infection. SCA detecting methods include (1) dot-ELISA/McAb anti-surface membrane antigen of adult worms (8SE4), (2) dot-ELISA/McAb anti-CCA (3D10), (3) Sandwich dot-ELISA/McAb anti-egg antigen (MG2), (4) Sandwich-ELISA/McAb anti-egg antigen (2H10), (5) Sandwich-ELISA/McAb anti-CAA (1B10). RESULTS: Using method (1) all of the 18 rabbits infected with monosexual cercariae were negative. Using method (2) only 1 rabbit harboured 133 male worms showed positive. Using method (5) no SCA were detected in sera from female cercariae-infected rabbits but 3 out of 9 male cercariae-infected rabbits showed positive reaction. SCA detecting results from rabbits treated with allyl thiourea: both method (1) and (3) showed negative in rabbits treated from 19 d (p.i.) but all gave positive reaction in 6-7 wk in rabbits treated from 46 d (p.i.). There were all negative detected by using method (4) and all positive at 6 wk (p.i.) detected by method (5) in rabbits of 3 groups whether treated or non-treated. CONCLUSION: Various test/probe systems have different efficacy in detecting SCA but (5) of the methods (1)(2) tests/probe systems could not detect SCA in rabbits with single female cercariae infection. From the egg formation inhibition study, the SCA detected by McAb 8SE4 dot-ELISA might be related to egg maturation. Whether the surface membrane antigen of adult worm has common antigenic component with egg antigen remains to be studied.

Animals↗
PubMed1998

[Histological changes of Cysticercus cellulosae under the action of proteinase of Omphalia lapidescens in vitro].

AIM: For the purpose of finding an anthelmintic with high efficacy, low toxicity and low cost, especially from the Chinese traditional medicines. METHODS: Forty Cysticercus cellulosae were separately incubated with proteinase from artificially fermented Omphalia lapidescens for 2 h, 4 h and 8 h, and then examined for histological changes by light microscopy and compared with those of proteinase from natural dry Omphalia lapidescens after similar treatment. RESULTS: Cysticercus cellulosae were morphologically and structurally impaired after exposure to the action of proteinase of Omphalia lapidescens, and the degree of impairment was proportionally paralleled to the duration of treatment. CONCLUSION: The anti-cysticercus effect of proteinase of Omphalia lapidescens and the homogeneity of proteinases from artificially fermented and natural Omphalia lapidescens were confirmed.

Animals↗
PubMed1998

[Observation on the infectivity of different densities of Plasmodium vivax to Anopheles sinensis].

AIM: To study the infectivity of different densities of P. vivax to An. sinensis. METHODS: Venous blood samples from 38 volunteers artificially infected with P. vivax of South-Yunnan collected at the time of initial parasitaemia, primary attack and relapse, were used to infect Anopheles sinensis by feeding on parasitaemia blood in vitro. The finding of oocysts in the mid-gut of the mosquito by microscope was taken as criteria of positive infection. RESULTS: P. vivax in the initial parasitaemia did not infect the mosquito. In the cases of primary attack, when the density of parasite was more than 100/microliter blood, the infectivity and intensity rose with the prolongation of the course of the disease and the increase in the density of the parasite. The infectivity of the P. vivax in the relapse cases was higher than in the primary attack cases, the mosquitoes could be infected at a parasite density of 1/microliter blood. The mosquitoes acquired a higher infection rate, a higher positive rate of mosquito mid-gut and a higher oocyst index in the primary attack cases whose parasite density were higher than 1,000/microliter blood and in the relapse cases whose parasite density were higher than 100/microliter blood. CONCLUSION: The P. vivax in the period of clinical attack was one of the most dangerous period of the spread of malaria. The cases of relapse were the more dangerous infective sources in malaria transmission.

Adult↗
PubMed1998

[Clinical pathology study of lingual carcinoma treatment with CDDP-AMS microcapsules through lingual artery].

11 patients with lingual carcinoma were treated by artery chemoembolization with 100 mg CDDP-AMS microcapsules (CDDP 13.6 mg) through lingual artery, and the average diameter of microcapsules was 56.3 microns. There was no obvious carcinoma cell or carcinoma tissue in pathologic specimens of the tissue after 4 weeks of treatment. After 5-6 weeks of chemoembolization, the clinical tumor ulcer disappeared, but it was difficult to judge the change of the tumor because of local fiber tissue. After 6 months, by observation of 4 patients whose tumors had not been excised, it was found that the infiltuation mass of primary carcinoma disappeared and tongue body became soft. It suggests that artery chemoembolization with CDDP-AMS microcapsules is an efficient way of lingual carcinoma treatment.

Adult↗