Biomedical subjects

H Li

Publications and source records attributed to H Li.

At least 1,153 records · Page 64Linked to original sources

PubMed1998

Ultrastructural evidence for vulnerability of hip region to fracture in the aged.

OBJECTIVE: To probe ultrastructurally into the mechanism why hip region is the predilected site of fracture in aged people, yet with femoral head exempted. METHODS: Proximal femoral ends were collected from 14 aged people, who had undergone endoprosthetic replacement for intra-capsular fracture of the femoral neck, and from 7 young men died of brain injury. These specimens were processed and subjected to scanning electron microscopic and transmission electron microscopic observation for bone resorption. RESULTS: As compared with the specimens from young men, there were three types of bone resorption in the proximal femoral ends of aged people, i.e. osteoclastic resorption and osteocytic resorption of the trabeculae of cancellous bone, and osteoclastic resorption of Haversian systems of the cortical bone. In the proximal femoral ends of the young men, the arch structures composed of trabeculae in the femoral head showed a round or roundish configuration, while those at the femoral head-neck junction revealed an oval profile and those in the femoral neck exhibited a narrow oval contour. In the proximal femoral ends of the aged people, the morphological characteristics of the trabecular arch remained unchanged despite overt bone resorption. CONCLUSIONS: Vulnerability of hip region to fracture in aged people is due to a decline in the structural strength of bone tissues of the proximal femoral ends resulting from osteoclastic and osteocytic resorption. The round and roundish trabecular arch of the femoral head can bear external forces from different directions, so that no fracture occurs here, while the oval and narrow oval arches at the head-neck junction and in the neck region fail to stand the horizontal external forces (abduction and adduction injuries), thereby fracture easily happens.

Adult↗

PubMed1998

[Analysis of spectral history of distort-product otoacoustic emissions in subjects of transient vertebrobasilar ischemic vertigo].

Analysis of the spectral history of distrot-product otoacoustic emissions (DPOAE) was performed to evaluate the cochlear function in subjects of vertebrobasilar transient ischemic vertigo (VBTIV). Thresholds of pure tone and acoustic reflex didn't increase while the amplitudes of DPOAE decreased, and the spectral history of DPOAE rippled dramastically. 36.8% of cases were found abnormal by the amplitudes and the spectral history of DPOAE. The results confirmed that those patients with mild disfunction of cochlea can be detected by the amplitude and spectral history of DPOAE, which may be useful to differentiate the region of VBTIV.

Aged↗

PubMed1998

[A survey of main allergic pollen in pollinosis in district of Wuhan].

In order to investigate the variety of the main allergic pollen of pollinosis in district of Wuhan city, A survey of airborne pollen was conducted with the nationally acknowledged standards and methods. While carrying out the survey, 200 pollinosis patients queried the main allergic pollen by their skin sensitivity test to identify the pollinosis occurrence time. The result showed that the airborne allergic pollen peak time each year occurs in two seasons: in spring from March to May and in autumn from August to October, in accord with the peak time of pollinosis in spring from April to June and in autumn from September to November. It revealed that the main resulting pollen in Wuhan during the spring-autumn, providing an important foundation for diagnose and treatment of pollinosis in Wuhan city.

Adult↗

PubMed1998

[The determinations of tumor necrosis factor and interleukin 6 in serum of patients with sudden sensorineural hearing loss].

Serum level of tumor necrosis factor(TNF alpha) and interleukin 6(IL-6) were determined in 32 patients with sudden sensorineural hearing loss (SSHL), 15 patients with progressive sensorineural hearing loss(PSHL) and 20 normal controls by using enzyme-linked immunosorbent assay. The results showed that plasma TNF alpha and IL-6 levels in patients with SSHL were higher than those in PSHL group and in normal control group (P < 0.05). The study suggested that the TNF alpha and IL-6 played an important role in the pathogenesis of SSHL.

Adolescent↗

PubMed1998

Hypercoagulable state and disseminated intravascular coagulation following an effective chemotherapy in tumor-bearing rats.

OBJECTIVE: To detect the changes in blood coagulation system and clarify the related mechanisms of chemotherapy-induced disseminated intravascular coagulation. METHODS: Changes in blood coagulation system and immunohistochemistry for fibrinogen were investigated in six groups of rats designed for different purposes. RESULTS: Decreased platelet count, prolonged prothrombin time and active partial thromboplastin time, elevated fibrinogen level, and decreased antithrombin were observed in the rats receiving a newly developed chemotherapy (NDC group), in which mitomycin C was administered intravenously together with angiotensin. Accumulation of fibrinogen and microthrombi in the blood vessels of multiple organs were also found in the NDC group by immunohistochemistry and histopathological examination. CONCLUSIONS: Rapid reduction of tumor mass induced by an effective chemotherapy could cause hypercoagulable state and disseminated intravascular coagulation.

Angiotensins↗

PubMed1998

[Microsurgical technique in the treatment of lumbar disc prolapse].

From 1983 to 1996, 354 cases of the lumbar intervertebral disc prolapse were treated by the microsurgical technique in our hospital. Among them, 330 cases were the simple prolapse of the lumbar intervertebral disc, the other 24 cases were complicated with the lateral recess stenosis. The application of the microsurgical technique has the advantage of its precision, shorter operative time, little surgical trauma, less bleeding, and quicker recovery. So the patients can usually get up and move around at an earlier time. Ninety-five percent of the patients acquired good results. It is suggested that the microsurgical technique is a relatively good operative method in the treatment of the lumbar intervertebral disc prolapse and the lateral recess stenosis.

Adolescent↗

PubMed1998

[Distribution of beta-thalassemia CD17 mutant(A-->T) in Shanxi, Gansu and Xinjiang along the "Silk Road"].

OBJECTIVE: To explore the distribution of beta-thalassemia CD17 mutant along the "Silk Road". METHOD: Polymerase chain reaction in combination with dot-blot hybridization of allele-specific oligonucleotide probes(PCR/ASO) was used. RESULTS: Twenty-four cases of beta-thalassemia with CD17 mutant(A-->T) including 3 cases in Shanxi, 20 cases in Gansu and 1 in Xinjiang, were found among 85 probands of beta-thalassemia. CONCLUSION: According to distribution characteristics of beta-thalassemia CD17 along the "Silk Road" of China, the CD17 mutant of beta-thalassemia might probably originate from the "Silk Road" in Longxi district of Gansu.

Adolescent↗

PubMed1998

[Study of hemoglobinopathy in Kashi district of Xinjiang].

OBJECTIVE: To study the hemoglobinopathy in Kashi district of Xinjiang. METHODS: The abnormal hemoglobin carriers were investigated with microelectrophoresis. The propositus of thalassemia were tested with two steps of screening technique. The structural analysis of abnormal hemoglobin was carried out with finger print analysis technique. Gene identification of thalassemia was performed with PCR/ASO technique. RESULTS: The incidence of hemoglobinopathy and thalassemia were 0.8% and 3.07%, respectively. Both were higher than the average level in the population of Xinjiang and the whole country. Two kinds of variant of HbJ Tashikuergan [alpha 19(AB1)Ala-->Glu] and HbD Punjab[P121(GH4)Glu-->Gln] were found in the structural analysis of 8 cases of abnormal hemoglobin. Four kinds of mutation: CD8(-AA),CD8/9(+G),CDs41/42(-TTCT) and IVS-I-5(G-->C) were revealed at the gene identification of 10 propositus of beta-thalassemia. HbJ Tashikuergan was firstly reported in the world, and CD8(-AA) and CDs8/9(+G) were firstly discovered in China. CONCLUSION: The types and distribution of abnormal hemoglobin and thalassemia in Kashi district were different from those in other areas of our country, and also unsimilar to our neighbour countries.

Adolescent↗

PubMed1998

[Zymographic analysis of MMPs in human carcinoma cell lines with different metastasis potential].

OBJECTIVE: To investigate the correlation between matrix metalloproteinases (MMPs) activities and metastatic potential of several groups of human carcinoma cells with different metastatic potential. METHODS: Several groups of human carcinoma cell lines (human lung carcinoma, human prostatic carcinoma and melanoma) with different metastatic potential were selected. By using cell culture, collection and concentration of conditioned media and zymographic analysis method the difference of MMPs production and activity among those cell lines were detected. RESULTS: The MMPs production capabilities of carcinoma cells rose following the increase of their invasive and metastatic potentials: that of PG is much higher than PAa's, and BE1 is higher also than CL3 and LH7. Advanced stage melanoma cell WM983a and WM451 product MMP-9, but primary stage cells don't. There are MMP-9 in the conditioned medium of prostatic carcinoma cell PC-3M, the metastatic clone of PC-3 which don't express MMP-9. CONCLUSION: The expression of MMPs especially MMP-9 of carcinoma cells is closely correlated to the metastatic and invasive potential.

Adenocarcinoma↗

PubMed1998

[Study on TGF beta 1, TGF beta 2, TGF beta 3 expression in the chick basilar papilla following gentamicin toxicity].

The beta-type transforming growth factors (TGF beta s) are secreted proteins, which play an important role in regulation of cell proliferation and differentiation in the embryonic inner ear. In order to probe into the effect of TGF beta s on the hair cell regeneration, expression of TGF beta 1, TGF beta 2 and TGF beta 3 proteins were examined by using immunohistochemistry in the chicken basilar papilla during hair cell regeneration following gentamicin ototoxicity. Ten-day-old chickens received daily subcutaneous injection of gentamicin sulfate 50 mg/kg of ten consecutive days. The animals were allowed to survive 1,3,7,14,21 and 28 days before sacrifice and preparation for examination of the expression of TGF beta 1, TGF beta 2 and TGF beta 3 proteins. Immunostaining results demonstrated that TGF beta 2 and TGF beta 3 proteins were observed in the damaged region of basilar papilla. TGF beta 2 and TGF beta 3 proteins positive cells were limited to the lumenal nuclear layer within the damaged region. TGF beta 1 protein positive cell was not found in our study. These results indicated that TGF beta 2 and TGF beta 3 proteins might play a role in regulating proliferation of the supporting cells immigrated into the lumenal nuclear layer during hair cell regeneration.

Animals↗

PubMed1998

[The role of nitric oxide and nitric oxide synthase in the pathogenesis of asthma].

OBJECTIVE: To investigate the role of nitric oxide (NO) and nitric oxide synthase (NOS) in the pathogenesis of asthma. METHOD: 52 guinea pigs were randomly divided into four groups of 13 each: (1) asthmatic group (Group A): Dunkin-Hartley guinea-pigs were injected celiacly with 1 ml of 10% ovalbumin (OA). After 14 days, the animals were inhaled with an aerosol of 1% OA for 40-60 seconds for 10 days every other day; (2) Corticosteroid prevention group (Group CT): As above, just before the animals were inhaled with an aerosol, 0.5 mg/kg dexamethasone were injected celiacly; (3) N-nitro-L arginine prevention group (Group L): As Group A, just before the animals were inhaled with an aerosol, 0.4 mg/kg LNNA were injected celiacly; (4) Controls (Group C): and nitrate (NO2.-/NO3.-) levels in plasma, nitrite bronchoalveolar lavage fluid (BALF) and lung tissues were examined. At the same time, inducible nitric oxide synthase (iNOS) and constitute nitric oxide synthase (cNOS) activity levels in the lung tissues were examined, and the changes of cNOS in the guinea pig asthma model lung tissues were observed using histochemical detection. RESULT: All groups had no significant alteration of NO2.-/NO3.- in the plasma (P > 0.05). Group A had increased amounts of NO2.-/NO3.- in the BALF and in the lung tissues compared with the other groups (BALF: Group A 10.2 +/- 1.3, Group CT 7.2 +/- 1.1, Group L 7.3 +/- 1.3, Group C 6.2 +/- 0.8 mumol/L respectively, all of P < 0.01; the lung tissues: Group A 0.89 +/- 0.07, Group CT 0.16 +/- 0.09, Group L 0.24 +/- 0.09, Group C 0.18 +/- 0.05 nmol/mg respectively, all of P < 0.01). Group A also showed increased amounts of iNOS levels in the lung tissues than the other groups (Group A 59 +/- 18, Group CT 10 +/- 5, Group L 12 +/- 7, Group C 10 +/- 5 pmol/mg respectively, all of P < 0.01). Group L showed decreased amounts of cNOS levels in the lung tissues than the Group C (0.8 +/- 0.4, 1.2 +/- 0.4 fmol/mg, P < 0.05). While there were no significant alterations in the other groups (P > 0.05). Elevation of iNOS in the lung tissues was correlated with NO2-/NO3- in the BALF and in the lung tissues (r = 0.714, 0.842, respectively, P < 0.05, 0.01 respectively). NADPHd was found to be a histochemical marker reflecting cNOS activity. It was found that there was no marked alteration of cNOS activity in the Group A, Group CT and Group C, but lower in the Group L. CONCLUSION: There is increased production of iNOS in asthmatic guinea pigs, the iNOS produced could cause increased production of NO, and probably cause cytotoxicity and mediate airway hyperresponsiveness. NO and NOS may play an important role in the pathogenesis of asthma.

Animals↗

PubMed1998

[Determination of Ondansetron in injection by high performance capillary zone electrophoresis].

Ondansetron is a kind of drug which is used for the treatment of nausea and vomiting induced by cytotoxic chemotherapy and radiotherapy. In this paper, a method to determine Ondansetron in injection by high performance capillary zone electrophoresis is discussed. The injection was directly introduced into the capillary employing 40 mmol/L phosphate at pH 4.03 as buffer. The capillary used was 75 microns in i.d. and 47 cm in length. UV detection was set at 254 nm. The calibration curve showed good linearity. The recovery of Ondansetron ranged from 95%-98% with a coefficient of variation of 1.05%(n = 4). This method is simple, rapid and reliable.

Antiemetics↗

PubMed1998

[Effect of trichosanthin of cell cycle and apoptosis of murine melanoma cells].

OBJECTIVE: To study the inhibitory effect of purified trichosanthin component on the proliferation of malignant melanoma. METHODS: The effect of purified trichosanthin component on the DNA synthesis, cell cycle and cell apoptosis of murine melanoma cells were detected by flowcytometry when cultured in vitro. RESULTS: The significant G0/G1 phase arrest was revealed by the increase of cells in G0/G1 phase and decrease of cells in S phase. The obvious apoptosis of melanoma cells was induced by purified trichosanthin component. G0/G1 phase arrest was highly correlated with apoptosis (r = 0.8705). CONCLUSIONS: The purified trichosanthin component can markedly inhibit melanoma cells by the suppression of DNA synthesis in S phase and cell mitosis as well as induction of cell apoptosis.

Animals↗

PubMed1998

[Long-term efficacy of plasma-derived hepatitis B vaccine among Chinese children--a 12-year follow-up].

OBJECTIVE: To evaluate protective efficacy of a plasma-derived hepatitis B vaccine during 12 years after vaccination. METHODS: A 12-year follow-up of 350 children who participated a randomized, double blind, placebo-controlled trial in 1982. RESULTS: During the 12 years after vaccination, more vaccinated children had anti-HBs of 10 S/N ratios or more than did the controls. At the 12 years 46.1% of the vaccinated and 33.9% of the controls retained anti-HBs levels of S/N ratios > or = 10 (P < 0.05). However, at 12 years median S/N ratio of anti-HBs of the vaccinated children with detectable anti-HBs was lower than that of the controls (15 vs 36, P < 0.0001). Protective efficacy against HBsAg carriage status at 5-10 years after vaccination varied from 83% to 100%. At 12 years, 14 chronic HBsAg carriers were identified among the controls, whereas only 1 case was noted in the vaccinated group (7.6% vs 0.6%, P < 0.01), account for an efficacy of 92%. CONCLUSIONS: Protective efficacy against chronic HBsAg carriage was satisfactory at 12 years after vaccination. No evidence was showed to support a booster of hepatitis B vaccine at this time.

Child, Preschool↗

PubMed1998

[Persistence of HB vaccine immune protection and response to hepatitis B booster immunization].

OBJECTIVE: To determine the persistence of immune protection of China-made, plasma-derived HBV vaccine in infants and the time table of booster immunization. METHODS: Serological effect of 7-year follow-up study and antibody response of an experimental study on booster were detected using radioimmunoassay. RESULTS: The protective anti-HBs positive rates of 1,018 children, who were vaccinated according to the regimen of three doses of 10 micrograms HBV vaccine, declined from 75.0% during the first two years to 48.2% at 7 years after the first dosages, however, the positive rates for HBsAg and anti-HBc always fluctuated at low frequency. 144 children aged 6 or 7 years old, who received the HBV vaccine in their infancy and were negative for both HBsAg and anti-HBc before booster, were selected from 1,018 children of the follow-up study and boosted with 1 microgram (intradermally) or 2 micrograms (hypodemically) HBV vaccine. Their anti-HBs GMT and anti-HBs positive rate were 190.6 mIU/ml and 89.6% at one month after booster, significantly higher than 14.7 mIU/ml and 54.9% before booster (P < 0.01), and declined back to 25.3 mIU/ml and 75.5% at 12th month; among 65 children with the anti-HBs negative before booster, 40 of them had the level of anti-HBs > or = 100 mIU/ml one month after booster, suggesting the retention of immunological memory in most of them. CONCLUSIONS: No need for revaccination against hepatitis B at 7th year after the initial immunization due to better persistence of immune protection of the vaccine.

Child↗

PubMed1998

[Rapid determination of active constituents in compound ibuprofen tablets by capillary zone electrophoresis].

A rapid capillary zone electrophoresis (CZE) method for the determination of ibuprofen and pseudoephedrine hydrochloride in compound ibuprofen tablets has been developed. CZE was performed in 0.025 mol/L phosphate buffer solution (pH 8.1). The two compounds were completely separated within 3 minutes, and quantified with a UV-detector at 210 nm by means of external standard method. Relative standard deviations of 2.9% for pseudoephedrine hydrochloride and 1.9% for ibuprofen were achieved for 11 determinations of the sample solution containing 9.5 mg/L pseudoephedrine hydrochloride and 66.7 mg/L ibuprofen, and recoveries of 103.1% and 97.6% were obtained respectively for the spiked pseudoephedrine hydrochloride and ibuprofen. The proposed method has been applied for the analysis of compound ibuprofen tablets, and the analytical results are in good agreement with those obtained by HPLC method.

Drug Compounding↗

PubMed1998

[Origin of nitric oxide synthase positive nerve fibers at zusanli area in rats].

OBJECTIVE: To find the origin of nitric oxide synthase (NOS) positive nerve fiber at the area around Zusanli point(St. 36). METHODS: The combined method of retrograde transport of horse radish peroxidase and nicotinamide adenosine dinucleotide phosphate diaphorase were adopted. RESULTS: Some peripheral processes of NOS positive neurons were distributed in the Zusanli point from the ganglia of L4 to S1, and some were projected from the lamina IX of L4 to S1 in spinal cord. CONCLUSION: The distributions of NOS positive nerve fibers in the, Zusanli area might be one of the morphological foundations of acupuncture effect of Zusanli point.

Acupuncture Points↗

PubMed1998

[Effect of Yangxue bushen tablet on ovarian function in animal model of Yang deficiency].

OBJECTIVE: To study the therapeutic mechanism of Yangxue Bushen tablet (tablet for nourishing blood and tonifying Kidney) on the Kidney deficiency type of functional amenorrhea and infrequent menstruation. METHODS: Applied with orimeten, models of Yang deficiency in female rabbits were made, ikaclomine was taken as the control drug. Group I was the group of animal model of Yang deficiency; Group II was the ikaclomine treatment group; Group III was the high dosage treatment group; Group IV was the low dosage group; Group V normal rabbit treated with distilled water. The functional effect of gonadal axis was evaluated by the changes of the signs of animal histomorphology of ovary and uterus, and the level of beta-endorphin. RESULTS: The animals of Yang deficiency in Group II, III and IV recovered as a whole, and the changes of different levels of folliculi, morphosis of endometria and deciduous vaginal epithelial cell in group II, III and IV were more obviously than those in group I and V (P < 0.05). The content of beta-endorphin in blood plasma in group I was 106.6 pg/ml, which was lower than those in the other groups (P < 0.05), and the contents of beta-endorphin in blood plasma, in group III, IV and II were similar to that in group V (P < 0.05). CONCLUSION: Yangxue Bushen tablet has the effect on regulating menstruation by regulating the function of gonadal axis, regulating and promoting the ovarian function.

Amenorrhea↗