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Biomedical subjects

H Li

Publications and source records attributed to H Li.

At least 955 records · Page 53Linked to original sources
PubMed1999

[One stage surgical repair for complex cranio-orbital injury].

OBJECTIVE: To approach clinical effect of early stage surgery on complex cranio-orbital injury. METHODS: Surgical procedures included removal of intracranial hematoma, contused and necrotic brain tissues, correction of cranio-orbital deformity, and repair of dure mater. RESULTS: Early evaluation was completed in 6 weeks, and late evaluation was made in 6 months after surgery according to the scale of Jennett and Bond's plastic surgical outcome and Glasgow outcome scale (GOS). Six weeks after surgery, GOS grade I was observed in 6 patients and GOS grade II in 3 patients. Excellent scale of Jennett and Bond was seen in 4 patients and good scale in 3 patients. Cerebrospinal leakage was effectively obstructed in 5 patients. CONCLUSIONS: Early stage repair for complex cranio-orbital injury may be conducive to improving neural function and decreasing intracranial infection.

Adult↗
PubMed1999

[The effect of sandostatin and 5-fluorouracil on apoptosis in breast cancer].

OBJECTIVE: To investigate the effect of 5-FU plus sandostatin on apoptosis in breast cancer. METHODS: Breast cancer samples from 16 patients were treated with 5-FU (10 microg/ml) and sandostatin (0.1 microg/ml) in vitro using the in situ terminal deoxynucleotidyl transferase assay (TDT) to detect apoptosis. The patients were female (mean age 54.4 years). In the 16 patients, 10 had positive estrogen receptor (ER) and positive progestogen receptor (PR), and the rest had negative ER and PR. RESULTS: 18 hours after addition of 5-FU, sandostatin and both, the apoptotic rate of tumor cells was (21.8 +/- 6.6)%, (14.3 +/- 8.8)%, and (26.7 +/- 6.9)% respectively. When samples were divided into 2 groups according to the sensitivity of ER and PR, the apoptotic rate of 5-FU plus sandostatin was (32.2 +/- 7.9)%, in positive ER and PR group, but (20.5 +/- 5.5)% in negative ER and PR group (P < 0.05). CONCLUSIONS: 5-FU plus sandostatin is more sensitive in the positive ER and PR group than in the negative ER and PR group. 5-FU plus sandostatin would be helpful in the biotherapy of breast cancer patients with positive ER and PR.

Adult↗
PubMed1999

[Expression and regulation of p16 in osteosarcoma].

OBJECTIVE: To study the expression and regulation of p16 and related factors in osteosarcoma. METHODS: The levels of p16 protein and related factors were explored by Western blot analysis in 12 cases of osteosarcoma tissues and in cells of different phases of osteosarcoma cell line OS732. The status of p16 protein in osteosarcoma cells was revealed by immunofluorescence analysis. RESULTS: p16 protein was detected in 10 of 12 osteosarcoma tissues. pRb was found in all of the specimens, but 8 of them were hyperphosphorylated. p16 protein was the least in G1 phase. Localized in cytoplasm during G1 phase, p16 protein gathered around nuclear envelope in S phase and entered the nucleus in G2 phase. CONCLUSIONS: The expression rate of p16 protein was 83.3% in osteosarcoma tissues. The expression of p16 protein was unable to inhibit the phosphorylation of pRb in most osteosarcomas; p16 protein was extremely low in G1 phase, presumably it was sufficient to inhibit the phosphorylation of pRb, causing abnormal proliferation of the cells.

Adolescent↗
PubMed1999

[Orbito-zygomatic approach for removal of tumors of the skull base].

OBJECTIVE: To evaluate the advantages and indications of orbito-zygomatic approach for removal of tumors of the skull base. METHODS: Fifteen patients with tumors of the skull base were operated on using obito-zygomatic approach. The advantages and indications of the approach were evaluated. RESULTS: In 7 cases of meningioma, total removal of the tumor was achieved in 6 cases, and subtotal removal in 1. In 4 cases of craniopharyngioma, the tumor were totally removed in 3 cases, and subtotally removed in 1. The tumor were totally removed in 2 cases of trigerminal neurilemoma and 1 case of germinoma. There was one operative death. All patients recovered except 3 who had minor extraocular parasis. CONCLUSIONS: This approach ensures additional space, and less cerebral retraction for easy access to skull base tumor, especially the large neoplasm of middle cranial base with or without orbital or posterior cranial base extension.

Adolescent↗
PubMed1999

[Studies on immunization strategies for hepatitis B in different endemic areas of China].

OBJECTIVE: To compare the cost-benefit of immunization with hepatitis B (HB) vaccine in different endemic areas of HB and to explore the optimal strategy for it. METHODS: Cost-benefit analysis and comprehensive weighted score analysis (CWSA) were used to screen the optimal immunization strategy for low, medium and heavy endemic areas of HB. RESULTS: Significant economic benefit was obtained in Long-An County, Guangxi Zhuang Autonomous Region, Shanghai and Jinan, Shandong Province from HB immunization programs for infants. The greatest benefit cost ratio (BCR) was provided by the low-dose immunization strategy, which was 49.91, 54.53 and 37.68, respectively for heavy, medium and low endemic areas of HB. The maximal net benefit could be obtained by the high-dose immunization strategy with better immune protective efficacy. CWSA showed that the low-dose immunization strategy was the optimal one for HB immunization. CONCLUSION: It is suggested that the low-dose immunization strategy be implemented in undeveloped areas to obtain a greater BCR, and the high-dose one be implemented in more developed areas to obtain more net benefit and lower prevalence of hepatitis B surface antigen carriage.

China↗
PubMed1999

[A study on relationship between hypertension and polymorphism of ACE gene in male Yi people in Liangshan Yi autonomous prefecture, Sichuan].

OBJECTIVE: To explore relationship between polymorphism of ACE gene and hypertension. METHODS: A cross-sectional study was conducted and PCR technique was used to analyze gene polymorphism. RESULTS: Genotypes DD, ID and II of ACE gene accounted for 13.0% and 9.7%, 50.9% and 48.4%, and 36.1% and 41.9%, respectively, in the normotensives and hypertensives. Frequencies of I and D alleles for ACE gene were 61.6% and 66.1%, and 38.4% and 33.9%, respectively, in the normotensives and hypertensives. There was no significant difference in distribution of II, ID and DD genotypes and I/D allele frequencies of ACE gene between the hypertensives and normotensives in male Yi people. CONCLUSION: Association between polymorphism of ACE gene and hypertension was uncertain.

Adult↗
PubMed1999

[Studies on male reproductive toxicity caused by hexavalent chromium].

OBJECTIVE: To study male reproductive toxicity caused by hexavalent chromium (Cr(VI +)). METHODS: Morphology of semen and spermatozoa was observed and chromium level in blood, chromium and zinc level in sperm plasma, leuteinizing hormone (LH) and follicle stimulating hormone (FSH) levels in serum were determined for male workers exposed to (Cr(VI +)) (CrO(3)X = 0.0195 mg/m(3)) with occupational epidemiologic investigation and laboratory analysis. RESULTS: For the exposed workers, their seminal counting was (52.21 +/- 45.51) x 10(9)/L, and zinc level in seminal plasma (4,811.85 +/- 1,401.88) micromol/L, significantly lower than those in controls (88.96 +/- 74.82) x 10(9)/L and (5 718.49 +/- 2 827.90) micromol/L, respectively, P < 0.05. Serum FSH was (7.34 +/- 6.88) IU/L in the exposed groups, significantly higher than that of controls (2.41 +/- 1.69) IU/L, P < 0.01. There were no significant difference in semen volume, semen liquefaction time, prevalence of teratospermia, serum Cr(VI +) and LH level in seminal plasma between exposed group and control group (P > 0.05). Prevalence of teratospermia was 0.24 +/- 0.09, and serum LH and FSH were (7.94 +/- 2.67) IU/L and FSH (9.33 +/- 6.47) IU/L, respectively, in workers exposed to high concentration of Cr(VI +) (CrO(3)X = 0.2351 mg/m(3)) significantly higher than those exposed to lower concentration of Cr(VI +) (CrO(3)X = 0.0172 mg/m(3)), with 0.17 +/- 0.06 of prevalence of teratospermia, (5.05 +/- 3.05) IU/L of LH, and (3.74 +/- 3.04) IU/L of FSH, respectively (P < 0.05). CONCLUSION: The results suggested that damage to convoluted seminiferous tubules epithelium, reduction of spermatozoa formation and increase in prevalence of teratospermia could be caused by exposure to certain concentration of Cr(VI +).

Adult↗
PubMed1999

[Effects of lead exposure on their brain-stem auditory evoked potential in children].

OBJECTIVE: To determine if low lead exposure can influence auditory development in children. METHODS: Children were divided into two groups, i.e., low (< 0.483 micromol/L) and high (>or= 0.483 micromol/L) blood lead levels with atomic spectrophotometry (AAS). Latencies and latent intervals between peak waves of their brain-stem auditory evoked potentials (BAEP) in the two groups were measured and compared. RESULTS: There was positive correlation between blood lead levels and BAEP in wave III at right ear, wave V at both right and left ears and latent intervals between peak waves I to V, with correlation coefficients of 0.2991, 0.2748, 0.3248, 0.2064 and 0.2412, respectively. There was significant difference in latencies of wave III at right ear, wave V at both right and left ears and latent intervals between peak waves I to V at both right and left ears and those between waves of III to V at right ear in the low blood lead group. Latencies and latent intervals between peak waves were longer in the high blood level group (with a mean of 0.78 micromol/L and a range of 0.58 to 1.42 micromol/L) than those in the low one (with a mean of 0.30 micromol/L and a range of 0.17 to 0.45 micromol/L). CONCLUSION: Elevated blood lead level could cause prolongation of latencies and latent intervals between peak waves of BAEP and slowdown of the nerve conduction velocity, and so auditory development was influenced.

Child↗
PubMed1999

[A preliminary study of loss of heterozygosity on chromosome 1p in primary hepatocellular carcinoma].

OBJECTIVE: Ten (10) loci on chromosome 1p were analyzed to detect LOH in 38 cases of hepatocellular carcinomas (HCC) in order to locate the deletion area and the possible deleted tumor suppressor gene (TSG) in HCC. METHODS: PCR based microsatellite instability analysis were used to detect LOH on chromosome 1p31 and 1p35-p36 in HCC. RESULTS: LOH were detected on chromosome 1p in 84.2% of HCC. High frequency of LOH (> 40%) were occurred at locus D1S186 on 1p31, locus D1S482 on 1p35 and loci D1S243, D1S160, D1S165 and D1S170 on 1p36. CONCLUSION: LOH occurred mostly on chromosome 1p31 and 1p35-p36 in HCC. There may be more than two TSGs which associated with development of HCC on chromosome 1p31 and 1p35-p36.

Adult↗
PubMed1999

[A preliminary report on two distinct tumor-suppressor regions on chromosome 1p36.2-p36.3 in human hepatocellular carcinomas].

OBJECTIVE: Both cytogenetic and molecular genetic analyses have unveiled non-random genomic alterations in the distal short arm of human chromosome 1 associated with a number of human malignancies including heptatocellular carcinoma (HCC). The aim of this investigation is to determine the precise region of deletion that may harbor the putative tumor suppressor genes in HCC. METHODS: For the study of the loss of heterozygosity (LOH), 38 cases of hepatitis B virus (HBV) associated HCC and their corresponding non-tumor liver tissues were detected with 43 microsatellite polymorphic markers particularly focusing on 1p. RESULTS: Twenty-eight of the 38 (74%) tumors showed LOH on at least one locus on 1p36.2-p36.3. Two distinct smallest common deleted regions (SCDRs) with different patterns of deletion were identified. The first SCDR is located on locus D1S2795 at 1p36.3, between loci D1S2145 and D1S2893. The second SCDR is located at 1p36.2, between loci D1S244 and D1S489. Both of the SCDRs have not been previously described in HCC. In addition, a region of possible homozygous deletion (HD) was also detected within the second SCDR between loci D1S1597 and D1S489 by comparative multiplex PCR. This is the first observation of a possible homozygous deletion on the distal short arm of chromosome 1 in HCC as well as in human tumors. CONCLUSIONS: The high-resolution deletion mapping of 1p36.2-p36.3 in HCC in this study confirmed the presence of two distinct regions of deletion. Our data strongly suggest the presence of at least two tumor suppressor regions on 1p36.2-p36.3 and play an important role in the pathogenesis of HBV associated HCC. These results also provide a basis for further studies directed at cloning potential tumor suppressor genes in these regions.

Carcinoma, Hepatocellular↗
PubMed1999

[The relationship between surgical staging, pathologic grading, operative type and postoperative recurrence in giant cell tumor of bone].

Sixty-eight surgical procedures of 58 patients with Giant Cell Tumor of Bone(GCT) were followed up over two years. The recurrence rate of GCT was Stage 1: 0 in 5, Stage 2: 15.4% in 39 and Stage 3: 37.5% in 24. It was significantly higher in Stage 3 than in Stage 2 (P < 0.05). The recurrence rate of GCT in Stage 2 was 30.8% in 13 intralesional curettage, and 0 in 17 wide or radical resection(P < 0.05). We conclude that the surgical staging of GCT has important value in predicting postoperative recurrence, in accurately evaluating the operative efforts and in guiding treatment, and that the operative type is a significant factor effecting on the recurrence of GCT.

Adolescent↗
PubMed1999

[Chemoembolization of primary hepatic carcinoma with portal vein tumor thrombus].

Forty cases of primary hepatic carcinoma(PHC) with portal vein tumor thrombus(PTT) underwent chemoembolization twice or more. The total effective rate was 67.5%. The survival rates of half, one, and three years were 75.0%, 12.5%, and 2.5% respectively after the treatment. The mean survival time was 9 months. The effective rate of the cases with the branch PTT was higher than other cases(P < 0.05). The influence caused by the average treatment interval had no significant difference(P > 0.05). It is suggested that (1) in case of controlling the applications, TAI + TAE is an effective therapy of PHC with PTT; (2) no matter where PTT is, an active management must be adopted; (3) the average treatment interval shall be decided according to conditions of the patients.

Adult↗
PubMed1999

[Study of mutagenesis of two procarcinogens in human amnion cells using a shuttle vector pWB1].

Using 2 x 10(-5) mol/ml beta-NF (beta-naphthoflavone) to induce FL cells to express cytochrome P450 isozymes at a reasonable level, we have studied the mutagenesis of two procarcinogens, aflatoxin B1 and benzo (alpha)pyrene, at four different doses. Except for their top doses, all other doses induced mutation in target gene SupF carried by plasmid pWB1 at the frequency of 10(-4), which is higher than the spontaneous mutation frequency (7.61 x 10(-6)). Aflatoxin B1 demonstrated normal effect-dose relationship. Meanwhile no mutant was detected in their correspondent negative controls. All the results suggest that shuttle vector pWB1 can be conveniently used for detection of procarcinogens and study of their mutagenesis mechanisms in human cells.

Aflatoxin B1↗
PubMed1999

[Establishment of a heterologous graft model for human breast infiltrating duct carcinoma in nude mice].

A heterologous graft model for human breast infiltrating duct carcinoma is reported in this paper. The grafts derived from an infiltration duct carcinoma of a patient's right breast and her metastatic lymphnode mass were transplanted into the breast pads of nude mice in 1996, and the carcinoma masses were found in breast pads 31 days later. By now, the grafts of carcinoma have been transplanted into nude mice for 15 passages with a full success in 59 mice and with the biological characteristics of the original breast carcinoma. This heterologous graft model was established for the first time in China and the results suggest it be a good model for further research of breast carcinoma.

Animals↗
PubMed1999

[Animal experiment of the ketamine effects on traumatic brain injury resulting from impact maxillofacial injury].

OBJECTIVE: To test the effects of ketamine (KET) on traumatic brain injury resulting from impact maxillofacial injury. METHODS: The rat models with middle serious traumatic brain injury were obtained by falling impaction at maxillofacial region of the rats, then the animals were treated with KET in the early stage, and the alteration of the neural function in those rats was observed at the same time. RESULTS: There was obvious difference between the rats in the KET-treating groups and the control group. It took about 2 days fewer than that of the control group for rats in the KET-treating group to recover. CONCLUSION: KET can shorten the recovery time of rats with brain injury resulting from impact maxillofacial injury.

Animals↗
PubMed1999

[Separation of alkylphenols in oil field water by micellar electrokinetic chromatography].

The abundance of alkylphenols in the system of water, oil and rock is of great geochemistry significance. The analysis of alkylphenols in oil field water using micellar electrokinetic chromatography with UV detection is described. The different variables that affect separation were studied and optimized. The results show that better separation was obtained with the borate concentration of 47.5 mmol/L, the SDS concentration of 70.0 mmol/L, pH 9.15, separation current of 100 microA, capillary temperature 20 degrees C. The organic additive(beta-CD) could partly improve separation. Water sample was introduced into the capillary by pressure of 5 kPa for several seconds. Detection wavelength was 200 nm and reference wavelength at 350 nm for all experiments. For the oil field water sample, the reproducibility of directly injected oil field water after filtration through a membrane filter of 0.45 micron pore size was far from good and it's concentrations of alkylphenols were too low to be detected. Therefore, a method of separation and enrichment by a column packed with tributyl phosphate(TBP) extraction resin was applied for analysis of TZ4 oil field water. Qualitative analysis show that there are phenol, o-cresol, m-cresol and p-cresol in this water sample and phenol is the main component and it's quantity is more than other alkylphenols. It's recoveries from the oil field water were 91.5%-95.3%. The results were satisfactory.

Chromatography, Micellar Electrokinetic Capillary↗