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Biomedical subjects

H Lenz

Publications and source records attributed to H Lenz.

At least 37 records · Page 2Linked to original sources
PubMed1988

[A laser surgery procedure in the treatment of tattooing with the argon ion laser].

A laser surgery procedure for treating tattoos using argon ion laser is described. The epithelial changes during continuous radiation are land marks for laser surgery procedure: 1. The coagulation zone, 2. its bubble-like rise, 3. its breaking, 4. the beginning of the carbonisation zone and 5. setting of a decreasing coagulation zone for smoothing the edges. From the very onset of the carbonisation zone radiation is discontinued and continued only in the periphery producing a decreasing coagulation zone. Punctiform and line-pattern laser application is employed; the untreated areas in between are treated in the following session. The average time between the sessions is 7 weeks and the average number of sessions for a tattoo is 3.1. The observation time is on an average 2 years and 1 month. 134 tattoos of 43 patients are treated by this technique. In 18.6% of the tattoos very good results are achieved, in 51.5% a good result, in 28.4% a satisfactory result and in 1.5% an unsatisfactory result. Hypopigmentations are seen in 19.4%, atrophic scars in 12.7%, hypertrophic scars in 14.9% and a rest of tattoos in 8.9%. Keloids are not seen. Using this argon laser technique for treating tattoos you will obtain better results with fewer side effects than in those studies which are accomplished treating tattoos with argon lasers and described in literature up to now.

Adolescent↗
PubMed1987

Cloning and nucleotide sequence of heavy- and light-chain cDNAs from a creatine-kinase-specific monoclonal antibody.

Determination of creatine kinase isoenzymes by inhibition assay is a useful tool for the diagnosis and monitoring of myocardial infarction. We have established several mouse hybridoma lines secreting monoclonal antibodies with creatine kinase M-subunit inhibitory capacity. One of the monoclonal antibodies (MAK33) inhibits creatine kinase-MM by 80% without influencing the activity of creatine kinase-MB. A combination of two monoclonal antibodies increased the inhibition of creatine kinase MM up to 99.4%. Poly(A) + RNA of hybridoma cells secreting MAK33 was isolated and used for cloning cDNA of both heavy and light chains of this antibody. Full-length cDNA clones were obtained by hybridization with gamma 1 and kappa constant region cDNA probes. The complete nucleotide sequences from the variable regions including signal peptide and part of the 5'-untranslated regions have been determined.

Amino Acid Sequence↗
PubMed1987

Reconstitution of functionally active antibody directed against creatine kinase from separately expressed heavy and light chains in non-lymphoid cells.

We report here for the first time reconstitution and secretion of functionally active antibody in non-lymphoid cells. Expression vectors for the light and the heavy chain of a monoclonal antibody directed against creatine kinase (EC 2.7.3.2) were introduced into COS and CHO Chinese hamster ovary dhfr- cells. Introduction of the expression vectors separately gave rise to immuno-reactive material in the culture supernatants, but only cotransfection of the expression plasmids resulted in secretion of protein with immuno-reactivity against antibodies directed against mouse heavy and light chains as well as specific antigen-binding affinity, as determined by enzyme-linked immunosorbent assay. Secreted kappa and gamma chains from reconstituted antibody were characterized by immunoadsorption and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. In COS cells, reconstituted antibody was transiently secreted; cotransfection of kappa and gamma chain expression plasmids with a dihydrofolate reductase (DHFR)-expression plasmid into CHO dhfr- cells gave rise to stable transformants secreting functionally active antibody.

Animals↗
PubMed1987

Denial of lineage: clinical investigation of 50 cases.

Fifty randomly selected Chinese schizophrenic patients with denial of lineage were investigated. This delusion concerns the generation of parents, sisters, but not the offspring. Twenty-four of them manifested delusion of high-ranking lineage or of distinguished leadership lineage. Denial of lineage, involving the delusion of high-ranking lineage and the delusion of leadership lineage, may occur at the onset or during the course of schizophrenia. The clinical characteristics of this delusion are described and its concept, diagnosis, differential diagnosis, course and also possible mechanism are discussed. One possible mechanism is of psychodynamical origin. Emotional conflicts resulting from dissatisfaction of the primary need of being loved from birth may contribute to the onset of the denial of lineage. The second possible mechanism is a sociocultural fact. Far Eastern culture is based on the clan whereas occidental culture is based on the self. Thus can observe the denial of lineage in the Far East, but in the occident we can experience instead the idea of surmounting our self so as to be god or now to experience the omnipotence in form of technical ideas, e.g. the rays.

Adult↗
PubMed1987

A monoclonal antibody that specifically inhibits human salivary alpha-amylase.

Our monoclonal antibody 88E8 specifically binds to and inhibits human salivary alpha-amylase (EC 3.2.1.1) and cross reacts negligibly with the pancreatic isoenzyme, inhibiting it by less than 1%, as compared with about 90% for the salivary isoenzyme. The antibody binds the S1 and S2 types of salivary alpha-amylase, but no pancreatic alpha-amylase isoenzyme forms. A pancreatic alpha-amylase assay involving 88E8 is under development, with alpha-glucosidase as auxiliary enzyme and p-nitrophenyl-maltoheptaoside as substrate; we give preliminary data on this assay. The assay has to be done by substrate start, because the antibody interacts very slowly with the enzyme in the presence of substrate. Assay results for pancreatic alpha-amylase correlate well with those for isoamylase assayed with use of an inhibitor from wheat-germ.

Antibodies, Monoclonal↗
PubMed1986

[Histologic changes in the epithelium of the respiratory mucosa of the lower turbinates following argon laser strip carbonization in vasomotor rhinitis].

The histological findings after argon-ion-laser-carbonization on the respiratory mucosa of the lower turbinates in vasomotor rhinitis are described directly after laser treatment and 6 years later. The laser surgical procedure without bleeding, the reduction of swelling of the lower turbinates, a slight diminishing of secretion, a significantly better nasal ventilation are demonstrated by the histological findings. The main characteristic histological findings are: a cuneiform laser lesion extending deep into the tissue to the venous plexus, four different typical tissue layers immediately after laser treatment, centrally the carbonization zone surrounded by a coagulation zone and next to this a honeycombed zone surrounded by a very small oedematous zone. Healing of the laser lesion is always complete. First of all, a squamous epithelium with subepithelial fibrosis and hypertrophy of the muscle cells in the venous plexus are seen. Half a year after laser carbonization metaplasia of the stratified squamous epithelium into pseudostratified cylindrical ciliated epithelium is seen. Occasionally, small islands of stratified squamous epithelium are visible.

Adolescent↗
PubMed1986

[Septumplasty. Histologic changes in resected and reimplanted autologous septal cartilage and bone following crush injury].

The histological findings of the crushed deviated septal cartilage and bone are described directly after reimplantation between the mucoperichondrium periostium in septoplasty, and after 11, 16, 24 and 26 months as well as after 6 and 6.5 years. Immediately after crushing, multiple fractures different in width and damage of single cartilage cells and bone cells are visible. The crushed reimplanted material shows partial absorption and partial regeneration especially near the fracture lines over a period of several years. Months and years after reimplantation, the following can be observed: vascularised connective tissue grows into the fractures, metaplastic bone production and a new growth of cartilage-like plates are visible at the crushed cartilage, primary and secondary bone production are visible at the crushed bone. Clinically, a stable plate of bone and cartilage is seen after septoplasty in the reimplantation zone. The importance of reimplantation of crushed material in septoplasty is emphasised.

Adult↗
PubMed1985

The black layer on the teeth of betel chewers: a light microscopic, microradiographic and electronmicroscopic study.

Ten teeth of betel chewing P'wo Karen, a hilltribe group of northern Thailand, were available for this morphologic study. The structural composition of the black layer on these teeth was studied by light microscopy, microradiography and by electronmicroscopy. Principally, the black layer on the teeth of betel chewers was identical with subgingival calculus which had been colored by specific polyphenols contained in the betel nut. However, a pellicle-like layer forming initially on enamel crystals was also observed. From the morphologic aspect of the black layer a specific anticariogenic property cannot be attested. Further studies on the chemical composition of this black layer may elucidate its probable anticariogenic and its carcinogenic properties relative to oral submucous fibrosis.

Areca↗
PubMed1985

Specific immunoassay of alpha-amylase isoenzymes in human serum.

A monoclonal antibody (66C7) was prepared that specifically binds human salivary amylase (EC 3.2.1.1); it cross reacts with human pancreatic amylase by less than 1%. Two procedures are described for determination of isoamylases in human serum with this antibody: an enzyme immunoassay for determining amylase of salivary origin, and a routine method in which this amylase is immunoprecipitated and the remaining (pancreatic) amylase activity is assayed. Results by the two methods correlate well.

Antibodies, Monoclonal↗
PubMed1985

[Nasal cycle using rhinomanometric measurements].

Observations on cyclical variations in the nasal resistance were made using active anterior rhinomanometry. About 80% of 40 healthy individuals showed a nasal cycle with a mean period of 2.5 h. Measurements were made during a 8 h at intervals of 30 min. Quantitative values for the variations of the flow volume V1.5 at 1.5 hPa are given and discussed.

Adolescent↗
PubMed1985

[Rhinomanometric measurements in obstructed nasal respiration before and following rhinosurgical interventions].

355 patients with nasal obstruction caused by different disease were treated by various nasal operations. Active anterior rhinomanometry was performed before and after operation, both before and after decongestion of the nasal mucosa. 254 patients were reassessed 6 to 9 months later. On average an increase of nasal airway was achieved from V 1.5 = 0.46 1/s before operation to 0.74 1/s after operation (before decongestion) and from V 1.5 = 0.55 1/s before operation to 0.83 1/s after operation (after decongestion of the nasal mucosa). The decongestion effect and the differences between the better worse nasal cavities showed a significant decrease after operation. Rhinomanometric evaluation of the various nasal operations is presented for patients with nasal blockade caused by various diseases.

Adolescent↗
PubMed1985

Comparison of different coefficients and units in rhinomanometry.

The total information of a rhinomanometric measurement is given by the simultaneous registration of flow and pressure during respiration. For characterization of such a measuring curve different methods and units are used. Since no normalized procedure exists up to now, it is difficult to compare measurements of different authors. In this work the relation between various procedures is discussed and methods are given for converting different quantities. The units of the International System are discussed.

Mathematics↗
PubMed1985

[Flattening of hypertrophic scars by punctiform argon ion laser coagulation in several sessions].

11 hypertrophic scars were treated by different argon ion laser techniques. The observation period is 0.5-3 years. Punctiform coagulation zones of 2-3 mm diameter, interspersed by untreated areas of scar tissue of 2 mm length, which are by lasered 6 weeks later in a second laser session, has proved most successful. Laser sessions are repeated in this manner until sufficient flattening of the hypertrophic scar is achieved. A low power density and very brief multiple interrupted radiation periods for controlling the thermal effect are useful. It is assumed that small superficial coagulation necroses produced by the argon ion laser will result in subsequent scar tissue being formed in other directions while decreasing the collagen formation, eventually resulting in flattening of the hypertrophic scar.

Adult↗
PubMed1985

[8 years' laser surgery of the inferior turbinates in vasomotor rhinopathy in the form of laser strip carbonization].

Between 1976 and 1984 more than 2000 patients with nasal obstruction due to vasomotor rhinitis were treated with the argon laser. The inferior turbinate was treated by laser-strip carbonisation over an area 3 to 5 cm long, 2 mm wide and 1 to 3 mm deep, surrounded by a 2 mm broad coagulation zone. 700 patients were invited to attend follow up and 411 turned up. The observation period was up to 5 years. 80% of the patients obtained a significantly improved or completely free nasal breathing. This result could be improved by 6% by further surgery. Advantages of the laser-strip carbonisation of the inferior turbinate are that it is a bloodless procedure which can be carried out accurately under vision, without nasal packing and it can be performed under local anesthesia without pain. Disadvantages are a longer healing period and temporary crust formation as in conchotomy, the high technical effort and cost of the laser. The end result after laser-strip carbonisation on the inferior turbinates was achieved after 1 1/2 to 2 years.

Humans↗
PubMed1985

[Active rhinomanometry with a minicomputer and printer].

A rhinomanometer is described which is coupled with a minicomputer and printer. The experimental results can be presented on a monitor and printed out via the printer. Besides plottings of rhinomanograms it is also possible to print out V1.5 values, the side difference, the decongestion effect, and other data of the patients in tabular form. Several measurements of the same patient, e. g. before and after decongestion, can be plotted and compared simultaneously. A nozzle system or a mask can be used. The advantages for the clinical application of this automatic computer system are described.

Adult↗
PubMed1984

[Endonasal surgical technic with the argon laser].

A unit consisting of an argon-ion-laser, a flexible beam guide system and a probe for endonasal lasersurgical applications is described. The instrument has been used successfully for several years. Endonasal lasersurgical techniques are described. Pathological tissue can be coagulated or carbonised in the form of a point or a line. In particular, lasersurgical techniques are described for surgery of the lower turbinates, for coagulation of Little's area in case of chronical nose bleeding, for coagulation or carbonisation of teleangiectasia in case of Morbus Rendu-Weber-Osler, for production of a nasoantral window, and for other applications.

Epistaxis↗
PubMed1984

[Tonsillectomy with a laser dissector. Preliminary report].

A so called laser-dissector with a flexible beam guide system for tonsillectomy is described. With this instrument the tissue is irradiated for a very short time resulting in a coagulation of the peritonsillar tissue with closure of the small vessels even before mechanical dissection is performed. A preliminary report of 10 patients is given. The main advantage is that the procedure could be performed without or with much less bleeding as in tonsillectomy without using vasoconstrictors.

Humans↗