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Biomedical subjects

H Lang

Publications and source records attributed to H Lang.

331 records · Page 19Linked to original sources
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[Porous hydroxylapatite ceramics with homologous osteoblasts from cell cultures for bone replacement].

In an animal model using 24 inbred Lewis rats the effect of the simultaneous implantation of in vitro cultivated osteoblasts and a porous hydroxyl apatite ceramic material on bone regenerations was studied. Bone tissue was harvested from 2 inbred rats and a cell line of osteoblasts was established. The osteoblasts were multiplied in cell cultures and after 3 passages inserted along with granular HA into monocortical femur defects in 24 rats of the same inbred line. After various times of observation the femurs were examined radiographically and bone growth was assessed histologically. No significant increase in bone growth rate was observed, although earlier studies in the same model showed a marked qualitative effect of reimplanted in vitro cultured osteoblasts on new bone formation. Considering that pre-incubation largely reduces the toxic effect of HA ceramics, the results of this study indicate that the activity of the implanted osteoblasts in the defect is prevented by the simultaneous implantation of replacement material and cells.

Animals↗
PubMedDate not supplied

[Bone regeneration following the implantation of osteoblasts from cell cultures].

An animal model utilizing 26 inbred rats was aimed at the question if the regeneration of bone tissue is enhanced by implantation of osteoblasts previously cultured in vitro. Bone tissue was harvested from inbred rats (Lewis) and a cell line of rat osteoblasts was established. The osteoblasts were cultured in vitro and multiplied in three passages. Characterization of the cells was by various methods. Monocortical defects were created in the distal portion of the rat femurs by drilling; and the cells, embedded in 25% bone gelatine, were reimplanted into these defects. After observation periods of varying lengths, the femurs were examined radiographically and bone growth in the defect area was evaluated histologically. The results of this study indicated that there was an initial effect of the reimplanted cultured osteoblasts on the bone growth pattern. At a later point in time this effect could no longer be demonstrated in the young, healthy rats.

Animals↗
PubMedDate not supplied

[Implantation of pyrolyzed calf bone along with osteoblasts cultured in vitro].

The regeneration of osseous tissues after simultaneous implantation of rat osteoblast-like cells cultured in vitro and pyrolyzed calf bone was investigated. In this study a cell line of rat osteoblast-like cells originating from explants of 2 inbred rats (Lewis) was established and cells were characterized. The osteoblast-like cells were cultured in vitro, embedded in bovine bone gelatine after 3 passages in culture and then implanted together with pyrolyzed calf bone in artificial femur defects of the inbred Lewis rats. After 10, 20, 40 and 80 days animals were sacrificed and the treated femora were taken for histological examination of bone growth. It was demonstrated that the cultured osteoblast-like cells showed an activity by forming extracortical bone, but had no measurable effect on bone growth pattern in the defects. Since our former animal study on reimplantation of osteoblast-like cells had revealed an effect on the cells on bone growth pattern, it is discussed whether the present lack of this effect can be explained by simultaneous implantation of xenogenic bone. In view of the importance of bone regeneration, autologous reimplantation of cultured bone cells deserves further investigation.

Animals↗
PubMedDate not supplied

[Does coping improve emotional adjustment? Results of a longitudinal study with bronchial carcinoma patients].

Coping research has been able to identify some maladaptive coping behaviours, but only little is known about favorable modes of adaptation. These findings have raised the issue of circularity, i.e. the question as to whether depressive ways of coping are actually the cause or rather the effect of emotional distress, whether those correlations reflect different aspects of psychological distress, or, even worse, confounded measures. Cross-sectional correlations are unable to solve this problem. The present study applies cross-lagged multiple regression analysis within a panel design. N = 120 newly diagnosed lung cancer patients who were admitted to a large tumor centre are included in the sample. Patients are assessed by self-reports and interviewer-ratings on measures of psychological adjustment (distress, hope) and coping (active confrontation, arguing with one's fate, consolation, distancing) at three times: Time 1 pre-treatment, Time 2 post-treatment, Time 3 recurrence. Results pertaining to the course of the measures of adjustment and coping are presented. Systematic deteriorating effects can be reveiled only at time 3. Stability coefficients prove to be lower than expected, especially with respect to the longer time interval. The cross-lagged pane 1 design allows testing alternative causal models. The time 1- time 2 results show, that arguing with one's fate may lead to future emotional distress, whereas hope is connected with reduced future arguing with one's fate. These short term findings are confirmed in the prediction of the time 3-data. In addition, consolation seems to have a negative impact on hope in the long term.(ABSTRACT TRUNCATED AT 250 WORDS)

Adaptation, Psychological↗